(Circular) (Six-base) MAPSORT of: pF1KB3683.seq Check: 8634 from: 1 to: 6635 pF1KB3683 6635 bp With 182 enzymes: SgfI * June 20, 2008 10:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1559 1559 Size: 6635 AatII G_ACGT'C Cuts at: 1884 1884 Size: 6635 AccI GT'mk_AC Cuts at: 1537 1974 2510 2919 3673 1537 Size: 437 536 409 754 4499 Fragments arranged by size: 4499 754 536 437 409 Acc65I G'GTAC_C Cuts at: 3651 3651 Size: 6635 AclI AA'CG_TT Cuts at: 6475 6475 Size: 6635 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 335 385 511 1925 2508 4460 4892 5639 5907 Size: 50 126 1414 583 1952 432 747 268 Cuts at: 5907 335 Size: 1063 Fragments arranged by size: 1952 1414 1063 747 583 432 268 126 50 AflIII A'CryG_T Cuts at: 2605 3987 5091 6068 2605 Size: 1382 1104 977 3172 Fragments arranged by size: 3172 1382 1104 977 AgeI A'CCGG_T Cuts at: 5023 5023 Size: 6635 AhdI GACnn_n'nnGTC Cuts at: 3041 3041 Size: 6635 AleI CACnn'nnGTG Cuts at: 3290 3290 Size: 6635 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1196 1228 1196 Size: 32 6603 AlwNI CAG_nnn'CTG Cuts at: 3524 3940 5507 3524 Size: 416 1567 4652 Fragments arranged by size: 4652 1567 416 ApaLI G'TGCA_C Cuts at: 5405 5405 Size: 6635 ApoI r'AATT_y Cuts at: 377 560 786 1062 1335 1369 1563 1680 1735 Size: 183 226 276 273 34 194 117 55 Cuts at: 1735 1761 1772 2857 2908 3639 5988 6244 377 Size: 26 11 1085 51 731 2349 256 768 Fragments arranged by size: 2349 1085 768 731 276 273 256 226 194 183 117 55 51 34 26 11 AseI AT'TA_AT Cuts at: 20 668 20 Size: 648 5987 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6635 AvaI C'yCGr_G Cuts at: 3655 3655 Size: 6635 BamHI G'GATC_C Cuts at: 645 3660 645 Size: 3015 3620 BanI G'GyrC_C Cuts at: 844 3651 4065 4313 4348 844 Size: 2807 414 248 35 3131 Fragments arranged by size: 3131 2807 414 248 35 BanII G_rGCy'C Cuts at: 3649 4679 3649 Size: 1030 5605 BbeI G_GCGC'C Cuts at: 4069 4317 4069 Size: 248 6387 BbsI GAAGACnn'nnnn_ Cuts at: 293 2486 293 Size: 2193 4442 BciVI GTATCCnnnnn_n' Cuts at: 1198 4533 5294 6635 1198 Size: 3335 761 1341 1198 Fragments arranged by size: 3335 1341 1198 761 BclI T'GATC_A Cuts at: 1470 1916 2250 2577 4155 1470 Size: 446 334 327 1578 3950 Fragments arranged by size: 3950 1578 446 334 327 BglI GCCn_nnn'nGGC Cuts at: 1693 1693 Size: 6635 BglII A'GATC_T Cuts at: 3340 4150 6187 3340 Size: 810 2037 3788 Fragments arranged by size: 3788 2037 810 BlpI GC'TnA_GC Cuts at: 320 3747 320 Size: 3427 3208 Bme1580I G_kGCm'C Cuts at: 4260 4353 5409 4260 Size: 93 1056 5486 Fragments arranged by size: 5486 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1473 3402 3847 3928 4263 6407 1473 Size: 1929 445 81 335 2144 1701 Fragments arranged by size: 2144 1929 1701 445 335 81 BmtI G_CTAG'C Cuts at: 1594 1594 Size: 6635 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5002 5002 Size: 6635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1520 2126 2657 1520 Size: 606 531 5498 Fragments arranged by size: 5498 606 531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 802 3811 4690 5182 5480 802 Size: 3009 879 492 298 1957 Fragments arranged by size: 3009 1957 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1174 1639 6339 1174 Size: 465 4700 1470 Fragments arranged by size: 4700 1470 465 BsaAI yAC'GTr Cuts at: 464 2608 3988 464 Size: 2144 1380 3111 Fragments arranged by size: 3111 2144 1380 BsaBI GATnn'nnATC Cuts at: 6 193 614 1891 1945 3446 4149 6186 6 Size: 187 421 1277 54 1501 703 2037 455 Fragments arranged by size: 2037 1501 1277 703 455 421 187 54 BsaHI Gr'CG_yC Cuts at: 1881 4066 4314 5016 6512 1881 Size: 2185 248 702 1496 2004 Fragments arranged by size: 2185 2004 1496 702 248 BsaWI w'CCGG_w Cuts at: 3824 4345 5023 5297 5444 5855 3824 Size: 521 678 274 147 411 4604 Fragments arranged by size: 4604 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2684 2714 2684 Size: 30 6605 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 284 848 1094 284 Size: 564 246 5825 Fragments arranged by size: 5825 564 246 BseYI C'CCAG_C Cuts at: 1294 1823 4060 4818 5395 5891 1294 Size: 529 2237 758 577 496 2038 Fragments arranged by size: 2237 2038 758 577 529 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 3151 3151 Size: 6635 BsiEI CG_ry'CG Cuts at: 104 4177 4223 5431 104 Size: 4073 46 1208 1308 Fragments arranged by size: 4073 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 420 2755 3649 4427 4617 5409 420 Size: 2335 894 778 190 792 1646 Fragments arranged by size: 2335 1646 894 792 778 190 BsmI GAATG_Cn' Cuts at: 640 1309 3061 3520 3563 640 Size: 669 1752 459 43 3712 Fragments arranged by size: 3712 1752 669 459 43 BsmBI CGTCTCn'nnnn_ Cuts at: 1574 1872 2800 1574 Size: 298 928 5409 Fragments arranged by size: 5409 928 298 Bsp1286I G_dGCh'C Cuts at: 420 2755 3649 4260 4353 4427 4617 4679 5409 Size: 2335 894 611 93 74 190 62 730 Cuts at: 5409 420 Size: 1646 Fragments arranged by size: 2335 1646 894 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 5855 5855 Size: 6635 BspHI T'CATG_A Cuts at: 2492 5811 2492 Size: 3319 3316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1559 3685 3899 4201 4582 1559 Size: 2126 214 302 381 3612 Fragments arranged by size: 3612 2126 381 302 214 BsrBI CCG'CTC Cuts at: 50 4927 4981 6465 6630 50 Size: 4877 54 1484 165 55 Fragments arranged by size: 4877 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2141 2450 4547 2141 Size: 309 2097 4229 Fragments arranged by size: 4229 2097 309 BsrFI r'CCGG_y Cuts at: 4633 4814 5023 4633 Size: 181 209 6245 Fragments arranged by size: 6245 209 181 BsrGI T'GTAC_A Cuts at: 3191 3191 Size: 6635 BssSI C'ACGA_G Cuts at: 3290 3344 4906 5264 3290 Size: 54 1562 358 4661 Fragments arranged by size: 4661 1562 358 54 BstAPI GCAn_nnn'nTGC Cuts at: 1789 3524 4012 1789 Size: 1735 488 4412 Fragments arranged by size: 4412 1735 488 BstBI TT'CG_AA Cuts at: 4996 4996 Size: 6635 BstYI r'GATC_y Cuts at: 1 645 987 1287 1355 1711 1842 1946 2070 Size: 644 342 300 68 356 131 104 124 Cuts at: 2070 3311 3340 3441 3660 4127 4150 4485 4731 Size: 1241 29 101 219 467 23 335 246 Cuts at: 4731 5743 5829 5841 6187 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1241 1012 644 467 449 356 346 342 335 300 246 219 131 124 104 101 86 68 29 23 12 Bsu36I CC'TnA_GG Cuts at: 1867 1867 Size: 6635 BtgI C'CryG_G Cuts at: 2514 3329 3396 2514 Size: 815 67 5753 Fragments arranged by size: 5753 815 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4585 4766 4929 4585 Size: 181 163 6291 Fragments arranged by size: 6291 181 163 BtsI GCAGTG_nn' Cuts at: 3878 3878 Size: 6635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 341 376 3453 3488 341 Size: 35 3077 35 3488 Fragments arranged by size: 3488 3077 35 35 DraI TTT'AAA Cuts at: 1562 3634 1562 Size: 2072 4563 DraIII CAC_nnn'GTG Cuts at: 2194 2194 Size: 6635 DrdI GACnn_nn'nnGTC Cuts at: 1533 2391 4341 5199 1533 Size: 858 1950 858 2969 Fragments arranged by size: 2969 1950 858 858 EaeI y'GGCC_r Cuts at: 2078 2447 4220 4394 4785 4812 6157 2078 Size: 369 1773 174 391 27 1345 2556 Fragments arranged by size: 2556 1773 1345 391 369 174 27 EagI C'GGCC_G Cuts at: 4220 4220 Size: 6635 EarI CTCTTCn'nnn_ Cuts at: 847 1239 2011 4658 4868 847 Size: 392 772 2647 210 2614 Fragments arranged by size: 2647 2614 772 392 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4843 5153 5299 6445 4843 Size: 310 146 1146 5033 Fragments arranged by size: 5033 1146 310 146 EcoICRI GAG'CTC Cuts at: 3647 3647 Size: 6635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 335 385 511 1520 1925 2126 2508 2657 4460 Size: 50 126 1009 405 201 382 149 1803 Cuts at: 4460 4892 5639 5907 335 Size: 432 747 268 1063 Fragments arranged by size: 1803 1063 1009 747 432 405 382 268 201 149 126 50 EcoO109I rG'GnC_Cy Cuts at: 3186 3301 3500 3774 3186 Size: 115 199 274 6047 Fragments arranged by size: 6047 274 199 115 EcoRI G'AATT_C Cuts at: 786 3639 786 Size: 2853 3782 EcoRV GAT'ATC Cuts at: 3000 3000 Size: 6635 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 541 4654 541 Size: 4113 2522 FspI TGC'GCA Cuts at: 4416 4416 Size: 6635 HaeII r_GCGC'y Cuts at: 4069 4317 5339 6318 4069 Size: 248 1022 979 4386 Fragments arranged by size: 4386 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 522 554 757 789 1272 1304 2300 2332 3027 Size: 32 203 32 483 32 996 32 695 Cuts at: 3027 3059 3129 3161 3273 3305 3325 3357 4477 Size: 32 70 32 112 32 20 32 1120 Cuts at: 4477 4507 4509 4539 522 Size: 30 2 30 2618 Fragments arranged by size: 2618 1120 996 695 483 203 112 70 32 32 32 32 32 32 32 32 30 30 20 2 HincII GTy'rAC Cuts at: 581 2920 3126 3674 581 Size: 2339 206 548 3542 Fragments arranged by size: 3542 2339 548 206 HindIII A'AGCT_T Cuts at: 1028 1028 Size: 6635 HpaI GTT'AAC Cuts at: 3126 3126 Size: 6635 KasI G'GCGC_C Cuts at: 4065 4313 4065 Size: 248 6387 KpnI G_GTAC'C Cuts at: 3655 3655 Size: 6635 MfeI C'AATT_G Cuts at: 2032 2441 2032 Size: 409 6226 MluI A'CGCG_T Cuts at: 6068 6068 Size: 6635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 971 1689 1809 5306 5490 971 Size: 718 120 3497 184 2116 Fragments arranged by size: 3497 2116 718 184 120 MscI TGG'CCA Cuts at: 2080 2449 4396 2080 Size: 369 1947 4319 Fragments arranged by size: 4319 1947 369 MslI CAynn'nnrTG Cuts at: 329 919 1160 1897 3290 3509 3513 4624 4751 Size: 590 241 737 1393 219 4 1111 127 Cuts at: 4751 329 Size: 2213 Fragments arranged by size: 2213 1393 1111 737 590 241 219 127 4 MspA1I CmG'CkG Cuts at: 237 1302 2506 2516 3746 4060 4420 5433 5678 Size: 1065 1204 10 1230 314 360 1013 245 Cuts at: 5678 237 Size: 1194 Fragments arranged by size: 1230 1204 1194 1065 1013 360 314 245 10 NaeI GCC'GGC Cuts at: 4816 4816 Size: 6635 NarI GG'CG_CC Cuts at: 4066 4314 4066 Size: 248 6387 NcoI C'CATG_G Cuts at: 3329 3396 3329 Size: 67 6568 NdeI CA'TA_TG Cuts at: 3266 3510 3266 Size: 244 6391 NgoMIV G'CCGG_C Cuts at: 4814 4814 Size: 6635 NheI G'CTAG_C Cuts at: 1590 1590 Size: 6635 NspI r_CATG'y Cuts at: 185 328 825 2618 2635 3572 3688 4188 5095 Size: 143 497 1793 17 937 116 500 907 Cuts at: 5095 185 Size: 1725 Fragments arranged by size: 1793 1725 937 907 500 497 143 116 17 PciI A'CATG_T Cuts at: 5091 5091 Size: 6635 PflMI CCAn_nnn'nTGG Cuts at: 2899 2899 Size: 6635 PmeI GTTT'AAAC Cuts at: 3634 3634 Size: 6635 PmlI CAC'GTG Cuts at: 2608 2608 Size: 6635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5774 5806 5774 Size: 32 6603 PpuMI rG'GwC_Cy Cuts at: 3186 3301 3500 3186 Size: 115 199 6321 Fragments arranged by size: 6321 199 115 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3184 3216 3184 Size: 32 6603 PstI C_TGCA'G Cuts at: 1785 3682 4367 1785 Size: 1897 685 4053 Fragments arranged by size: 4053 1897 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6635 PvuII CAG'CTG Cuts at: 237 2506 4060 4420 237 Size: 2269 1554 360 2452 Fragments arranged by size: 2452 2269 1554 360 RsrII CG'GwC_CG Cuts at: 4830 4830 Size: 6635 SacI G_AGCT'C Cuts at: 3649 3649 Size: 6635 SacII CC_GC'GG Cuts at: 2517 2517 Size: 6635 SalI G'TCGA_C Cuts at: 2918 3672 2918 Size: 754 5881 SapI GCTCTTCn'nnn_ Cuts at: 4658 4868 4658 Size: 210 6425 SbfI CC_TGCA'GG Cuts at: 3682 3682 Size: 6635 ScaI AGT'ACT Cuts at: 807 6152 807 Size: 5345 1290 SfcI C'TryA_G Cuts at: 33 299 307 1664 1781 2058 2507 2781 3678 Size: 266 8 1357 117 277 449 274 897 Cuts at: 3678 4363 5356 5547 33 Size: 685 993 191 1121 Fragments arranged by size: 1357 1121 993 897 685 449 277 274 266 191 117 8 SfoI GGC'GCC Cuts at: 4067 4315 4067 Size: 248 6387 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6635 SmaI CCC'GGG Cuts at: 3657 3657 Size: 6635 SmlI C'TyrA_G Cuts at: 781 3790 4705 5197 5459 781 Size: 3009 915 492 262 1957 Fragments arranged by size: 3009 1957 915 492 262 SpeI A'CTAG_T Cuts at: 67 2800 67 Size: 2733 3902 SphI G_CATG'C Cuts at: 185 328 825 2635 3572 3688 4188 185 Size: 143 497 1810 937 116 500 2632 Fragments arranged by size: 2632 1810 937 500 497 143 116 SspI AAT'ATT Cuts at: 3230 3230 Size: 6635 StyI C'CwwG_G Cuts at: 2217 3329 3396 3769 4123 2217 Size: 1112 67 373 354 4729 Fragments arranged by size: 4729 1112 373 354 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 4218 4351 4810 5019 4218 Size: 133 459 209 5834 Fragments arranged by size: 5834 459 209 133 TatI w'GTAC_w Cuts at: 805 3191 6150 805 Size: 2386 2959 1290 Fragments arranged by size: 2959 2386 1290 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6152 6184 6152 Size: 32 6603 Tth111I GACn'n_nGTC Cuts at: 2924 4432 2924 Size: 1508 5127 XbaI T'CTAG_A Cuts at: 3666 3666 Size: 6635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2088 4130 2088 Size: 2042 4593 XmaI C'CCGG_G Cuts at: 3655 3655 Size: 6635 XmnI GAAnn'nnTTC Cuts at: 524 683 3059 524 Size: 159 2376 4100 Fragments arranged by size: 4100 2376 159 ZraI GAC'GTC Cuts at: 1882 1882 Size: 6635 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PciI PflMI PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflII AlfI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI Bpu10I BsiWI BssHII BstEII BstXI BstZ17I ClaI EcoNI FseI FspAI NotI NruI NsiI PacI PasI PfoI PshAI PsiI PspOMI PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI