Restriction map for pF1KB3683
 (Circular) (Six-base) MAPSORT of: pF1KB3683.seq  Check: 8634  from: 1  to: 6635



pF1KB3683  6635 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1559    1559 
   Size:       6635
 
AatII G_ACGT'C

Cuts at:   1884    1884 
   Size:       6635
 
AccI GT'mk_AC

Cuts at:   1537    1974    2510    2919    3673    1537 
   Size:        437     536     409     754    4499

  Fragments arranged by size: 

                4499     754     536     437     409
 
Acc65I G'GTAC_C

Cuts at:   3651    3651 
   Size:       6635
 
AclI AA'CG_TT

Cuts at:   6475    6475 
   Size:       6635
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     385     511    1925    2508    4460    4892    5639    5907 
   Size:         50     126    1414     583    1952     432     747     268

Cuts at:   5907     335 
   Size:       1063

  Fragments arranged by size: 

                1952    1414    1063     747     583     432     268     126
                  50
 
AflIII A'CryG_T

Cuts at:   2605    3987    5091    6068    2605 
   Size:       1382    1104     977    3172

  Fragments arranged by size: 

                3172    1382    1104     977
 
AgeI A'CCGG_T

Cuts at:   5023    5023 
   Size:       6635
 
AhdI GACnn_n'nnGTC

Cuts at:   3041    3041 
   Size:       6635
 
AleI CACnn'nnGTG

Cuts at:   3290    3290 
   Size:       6635
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1196    1228    1196 
   Size:         32    6603
 
AlwNI CAG_nnn'CTG

Cuts at:   3524    3940    5507    3524 
   Size:        416    1567    4652

  Fragments arranged by size: 

                4652    1567     416
 
ApaLI G'TGCA_C

Cuts at:   5405    5405 
   Size:       6635
 
ApoI r'AATT_y

Cuts at:    377     560     786    1062    1335    1369    1563    1680    1735 
   Size:        183     226     276     273      34     194     117      55

Cuts at:   1735    1761    1772    2857    2908    3639    5988    6244     377 
   Size:         26      11    1085      51     731    2349     256     768

  Fragments arranged by size: 

                2349    1085     768     731     276     273     256     226
                 194     183     117      55      51      34      26      11
            
 
AseI AT'TA_AT

Cuts at:     20     668      20 
   Size:        648    5987
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6635
 
AvaI C'yCGr_G

Cuts at:   3655    3655 
   Size:       6635
 
BamHI G'GATC_C

Cuts at:    645    3660     645 
   Size:       3015    3620
 
BanI G'GyrC_C

Cuts at:    844    3651    4065    4313    4348     844 
   Size:       2807     414     248      35    3131

  Fragments arranged by size: 

                3131    2807     414     248      35
 
BanII G_rGCy'C

Cuts at:   3649    4679    3649 
   Size:       1030    5605
 
BbeI G_GCGC'C

Cuts at:   4069    4317    4069 
   Size:        248    6387
 
BbsI GAAGACnn'nnnn_

Cuts at:    293    2486     293 
   Size:       2193    4442
 
BciVI GTATCCnnnnn_n'

Cuts at:   1198    4533    5294    6635    1198 
   Size:       3335     761    1341    1198

  Fragments arranged by size: 

                3335    1341    1198     761
 
BclI T'GATC_A

Cuts at:   1470    1916    2250    2577    4155    1470 
   Size:        446     334     327    1578    3950

  Fragments arranged by size: 

                3950    1578     446     334     327
 
BglI GCCn_nnn'nGGC

Cuts at:   1693    1693 
   Size:       6635
 
BglII A'GATC_T

Cuts at:   3340    4150    6187    3340 
   Size:        810    2037    3788

  Fragments arranged by size: 

                3788    2037     810
 
BlpI GC'TnA_GC

Cuts at:    320    3747     320 
   Size:       3427    3208
 
Bme1580I G_kGCm'C

Cuts at:   4260    4353    5409    4260 
   Size:         93    1056    5486

  Fragments arranged by size: 

                5486    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1473    3402    3847    3928    4263    6407    1473 
   Size:       1929     445      81     335    2144    1701

  Fragments arranged by size: 

                2144    1929    1701     445     335      81
 
BmtI G_CTAG'C

Cuts at:   1594    1594 
   Size:       6635
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5002    5002 
   Size:       6635
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1520    2126    2657    1520 
   Size:        606     531    5498

  Fragments arranged by size: 

                5498     606     531
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    802    3811    4690    5182    5480     802 
   Size:       3009     879     492     298    1957

  Fragments arranged by size: 

                3009    1957     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1174    1639    6339    1174 
   Size:        465    4700    1470

  Fragments arranged by size: 

                4700    1470     465
 
BsaAI yAC'GTr

Cuts at:    464    2608    3988     464 
   Size:       2144    1380    3111

  Fragments arranged by size: 

                3111    2144    1380
 
BsaBI GATnn'nnATC

Cuts at:      6     193     614    1891    1945    3446    4149    6186       6 
   Size:        187     421    1277      54    1501     703    2037     455

  Fragments arranged by size: 

                2037    1501    1277     703     455     421     187      54
            
 
BsaHI Gr'CG_yC

Cuts at:   1881    4066    4314    5016    6512    1881 
   Size:       2185     248     702    1496    2004

  Fragments arranged by size: 

                2185    2004    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   3824    4345    5023    5297    5444    5855    3824 
   Size:        521     678     274     147     411    4604

  Fragments arranged by size: 

                4604     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2684    2714    2684 
   Size:         30    6605
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    284     848    1094     284 
   Size:        564     246    5825

  Fragments arranged by size: 

                5825     564     246
 
BseYI C'CCAG_C

Cuts at:   1294    1823    4060    4818    5395    5891    1294 
   Size:        529    2237     758     577     496    2038

  Fragments arranged by size: 

                2237    2038     758     577     529     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   3151    3151 
   Size:       6635
 
BsiEI CG_ry'CG

Cuts at:    104    4177    4223    5431     104 
   Size:       4073      46    1208    1308

  Fragments arranged by size: 

                4073    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    420    2755    3649    4427    4617    5409     420 
   Size:       2335     894     778     190     792    1646

  Fragments arranged by size: 

                2335    1646     894     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    640    1309    3061    3520    3563     640 
   Size:        669    1752     459      43    3712

  Fragments arranged by size: 

                3712    1752     669     459      43
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1574    1872    2800    1574 
   Size:        298     928    5409

  Fragments arranged by size: 

                5409     928     298
 
Bsp1286I G_dGCh'C

Cuts at:    420    2755    3649    4260    4353    4427    4617    4679    5409 
   Size:       2335     894     611      93      74     190      62     730

Cuts at:   5409     420 
   Size:       1646

  Fragments arranged by size: 

                2335    1646     894     730     611     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   5855    5855 
   Size:       6635
 
BspHI T'CATG_A

Cuts at:   2492    5811    2492 
   Size:       3319    3316
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1559    3685    3899    4201    4582    1559 
   Size:       2126     214     302     381    3612

  Fragments arranged by size: 

                3612    2126     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4927    4981    6465    6630      50 
   Size:       4877      54    1484     165      55

  Fragments arranged by size: 

                4877    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2141    2450    4547    2141 
   Size:        309    2097    4229

  Fragments arranged by size: 

                4229    2097     309
 
BsrFI r'CCGG_y

Cuts at:   4633    4814    5023    4633 
   Size:        181     209    6245

  Fragments arranged by size: 

                6245     209     181
 
BsrGI T'GTAC_A

Cuts at:   3191    3191 
   Size:       6635
 
BssSI C'ACGA_G

Cuts at:   3290    3344    4906    5264    3290 
   Size:         54    1562     358    4661

  Fragments arranged by size: 

                4661    1562     358      54
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1789    3524    4012    1789 
   Size:       1735     488    4412

  Fragments arranged by size: 

                4412    1735     488
 
BstBI TT'CG_AA

Cuts at:   4996    4996 
   Size:       6635
 
BstYI r'GATC_y

Cuts at:      1     645     987    1287    1355    1711    1842    1946    2070 
   Size:        644     342     300      68     356     131     104     124

Cuts at:   2070    3311    3340    3441    3660    4127    4150    4485    4731 
   Size:       1241      29     101     219     467      23     335     246

Cuts at:   4731    5743    5829    5841    6187       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1241    1012     644     467     449     356     346     342
                 335     300     246     219     131     124     104     101
                  86      68      29      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1867    1867 
   Size:       6635
 
BtgI C'CryG_G

Cuts at:   2514    3329    3396    2514 
   Size:        815      67    5753

  Fragments arranged by size: 

                5753     815      67
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4585    4766    4929    4585 
   Size:        181     163    6291

  Fragments arranged by size: 

                6291     181     163
 
BtsI GCAGTG_nn'

Cuts at:   3878    3878 
   Size:       6635
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    341     376    3453    3488     341 
   Size:         35    3077      35    3488

  Fragments arranged by size: 

                3488    3077      35      35
 
DraI TTT'AAA

Cuts at:   1562    3634    1562 
   Size:       2072    4563
 
DraIII CAC_nnn'GTG

Cuts at:   2194    2194 
   Size:       6635
 
DrdI GACnn_nn'nnGTC

Cuts at:   1533    2391    4341    5199    1533 
   Size:        858    1950     858    2969

  Fragments arranged by size: 

                2969    1950     858     858
 
EaeI y'GGCC_r

Cuts at:   2078    2447    4220    4394    4785    4812    6157    2078 
   Size:        369    1773     174     391      27    1345    2556

  Fragments arranged by size: 

                2556    1773    1345     391     369     174      27
 
EagI C'GGCC_G

Cuts at:   4220    4220 
   Size:       6635
 
EarI CTCTTCn'nnn_

Cuts at:    847    1239    2011    4658    4868     847 
   Size:        392     772    2647     210    2614

  Fragments arranged by size: 

                2647    2614     772     392     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4843    5153    5299    6445    4843 
   Size:        310     146    1146    5033

  Fragments arranged by size: 

                5033    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   3647    3647 
   Size:       6635
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     385     511    1520    1925    2126    2508    2657    4460 
   Size:         50     126    1009     405     201     382     149    1803

Cuts at:   4460    4892    5639    5907     335 
   Size:        432     747     268    1063

  Fragments arranged by size: 

                1803    1063    1009     747     432     405     382     268
                 201     149     126      50
 
EcoO109I rG'GnC_Cy

Cuts at:   3186    3301    3500    3774    3186 
   Size:        115     199     274    6047

  Fragments arranged by size: 

                6047     274     199     115
 
EcoRI G'AATT_C

Cuts at:    786    3639     786 
   Size:       2853    3782
 
EcoRV GAT'ATC

Cuts at:   3000    3000 
   Size:       6635
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    541    4654     541 
   Size:       4113    2522
 
FspI TGC'GCA

Cuts at:   4416    4416 
   Size:       6635
 
HaeII r_GCGC'y

Cuts at:   4069    4317    5339    6318    4069 
   Size:        248    1022     979    4386

  Fragments arranged by size: 

                4386    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    522     554     757     789    1272    1304    2300    2332    3027 
   Size:         32     203      32     483      32     996      32     695

Cuts at:   3027    3059    3129    3161    3273    3305    3325    3357    4477 
   Size:         32      70      32     112      32      20      32    1120

Cuts at:   4477    4507    4509    4539     522 
   Size:         30       2      30    2618

  Fragments arranged by size: 

                2618    1120     996     695     483     203     112      70
                  32      32      32      32      32      32      32      32
                  30      30      20       2
 
HincII GTy'rAC

Cuts at:    581    2920    3126    3674     581 
   Size:       2339     206     548    3542

  Fragments arranged by size: 

                3542    2339     548     206
 
HindIII A'AGCT_T

Cuts at:   1028    1028 
   Size:       6635
 
HpaI GTT'AAC

Cuts at:   3126    3126 
   Size:       6635
 
KasI G'GCGC_C

Cuts at:   4065    4313    4065 
   Size:        248    6387
 
KpnI G_GTAC'C

Cuts at:   3655    3655 
   Size:       6635
 
MfeI C'AATT_G

Cuts at:   2032    2441    2032 
   Size:        409    6226
 
MluI A'CGCG_T

Cuts at:   6068    6068 
   Size:       6635
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    971    1689    1809    5306    5490     971 
   Size:        718     120    3497     184    2116

  Fragments arranged by size: 

                3497    2116     718     184     120
 
MscI TGG'CCA

Cuts at:   2080    2449    4396    2080 
   Size:        369    1947    4319

  Fragments arranged by size: 

                4319    1947     369
 
MslI CAynn'nnrTG

Cuts at:    329     919    1160    1897    3290    3509    3513    4624    4751 
   Size:        590     241     737    1393     219       4    1111     127

Cuts at:   4751     329 
   Size:       2213

  Fragments arranged by size: 

                2213    1393    1111     737     590     241     219     127
                   4
 
MspA1I CmG'CkG

Cuts at:    237    1302    2506    2516    3746    4060    4420    5433    5678 
   Size:       1065    1204      10    1230     314     360    1013     245

Cuts at:   5678     237 
   Size:       1194

  Fragments arranged by size: 

                1230    1204    1194    1065    1013     360     314     245
                  10
 
NaeI GCC'GGC

Cuts at:   4816    4816 
   Size:       6635
 
NarI GG'CG_CC

Cuts at:   4066    4314    4066 
   Size:        248    6387
 
NcoI C'CATG_G

Cuts at:   3329    3396    3329 
   Size:         67    6568
 
NdeI CA'TA_TG

Cuts at:   3266    3510    3266 
   Size:        244    6391
 
NgoMIV G'CCGG_C

Cuts at:   4814    4814 
   Size:       6635
 
NheI G'CTAG_C

Cuts at:   1590    1590 
   Size:       6635
 
NspI r_CATG'y

Cuts at:    185     328     825    2618    2635    3572    3688    4188    5095 
   Size:        143     497    1793      17     937     116     500     907

Cuts at:   5095     185 
   Size:       1725

  Fragments arranged by size: 

                1793    1725     937     907     500     497     143     116
                  17
 
PciI A'CATG_T

Cuts at:   5091    5091 
   Size:       6635
 
PflMI CCAn_nnn'nTGG

Cuts at:   2899    2899 
   Size:       6635
 
PmeI GTTT'AAAC

Cuts at:   3634    3634 
   Size:       6635
 
PmlI CAC'GTG

Cuts at:   2608    2608 
   Size:       6635
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5774    5806    5774 
   Size:         32    6603
 
PpuMI rG'GwC_Cy

Cuts at:   3186    3301    3500    3186 
   Size:        115     199    6321

  Fragments arranged by size: 

                6321     199     115
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3184    3216    3184 
   Size:         32    6603
 
PstI C_TGCA'G

Cuts at:   1785    3682    4367    1785 
   Size:       1897     685    4053

  Fragments arranged by size: 

                4053    1897     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6635
 
PvuII CAG'CTG

Cuts at:    237    2506    4060    4420     237 
   Size:       2269    1554     360    2452

  Fragments arranged by size: 

                2452    2269    1554     360
 
RsrII CG'GwC_CG

Cuts at:   4830    4830 
   Size:       6635
 
SacI G_AGCT'C

Cuts at:   3649    3649 
   Size:       6635
 
SacII CC_GC'GG

Cuts at:   2517    2517 
   Size:       6635
 
SalI G'TCGA_C

Cuts at:   2918    3672    2918 
   Size:        754    5881
 
SapI GCTCTTCn'nnn_

Cuts at:   4658    4868    4658 
   Size:        210    6425
 
SbfI CC_TGCA'GG

Cuts at:   3682    3682 
   Size:       6635
 
ScaI AGT'ACT

Cuts at:    807    6152     807 
   Size:       5345    1290
 
SfcI C'TryA_G

Cuts at:     33     299     307    1664    1781    2058    2507    2781    3678 
   Size:        266       8    1357     117     277     449     274     897

Cuts at:   3678    4363    5356    5547      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1357    1121     993     897     685     449     277     274
                 266     191     117       8
 
SfoI GGC'GCC

Cuts at:   4067    4315    4067 
   Size:        248    6387
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6635
 
SmaI CCC'GGG

Cuts at:   3657    3657 
   Size:       6635
 
SmlI C'TyrA_G

Cuts at:    781    3790    4705    5197    5459     781 
   Size:       3009     915     492     262    1957

  Fragments arranged by size: 

                3009    1957     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67    2800      67 
   Size:       2733    3902
 
SphI G_CATG'C

Cuts at:    185     328     825    2635    3572    3688    4188     185 
   Size:        143     497    1810     937     116     500    2632

  Fragments arranged by size: 

                2632    1810     937     500     497     143     116
 
SspI AAT'ATT

Cuts at:   3230    3230 
   Size:       6635
 
StyI C'CwwG_G

Cuts at:   2217    3329    3396    3769    4123    2217 
   Size:       1112      67     373     354    4729

  Fragments arranged by size: 

                4729    1112     373     354      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   4218    4351    4810    5019    4218 
   Size:        133     459     209    5834

  Fragments arranged by size: 

                5834     459     209     133
 
TatI w'GTAC_w

Cuts at:    805    3191    6150     805 
   Size:       2386    2959    1290

  Fragments arranged by size: 

                2959    2386    1290
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6152    6184    6152 
   Size:         32    6603
 
Tth111I GACn'n_nGTC

Cuts at:   2924    4432    2924 
   Size:       1508    5127
 
XbaI T'CTAG_A

Cuts at:   3666    3666 
   Size:       6635
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2088    4130    2088 
   Size:       2042    4593
 
XmaI C'CCGG_G

Cuts at:   3655    3655 
   Size:       6635
 
XmnI GAAnn'nnTTC

Cuts at:    524     683    3059     524 
   Size:        159    2376    4100

  Fragments arranged by size: 

                4100    2376     159
 
ZraI GAC'GTC

Cuts at:   1882    1882 
   Size:       6635
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NspI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AlfI     ApaI     AscI    AvrII     BaeI    BbvCI
     BcgI    BfrBI    BmgBI   Bpu10I    BsiWI   BssHII   BstEII    BstXI
  BstZ17I     ClaI    EcoNI     FseI    FspAI     NotI     NruI     NsiI
     PacI     PasI     PfoI    PshAI     PsiI   PspOMI    PspXI    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI
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