Restriction map for
pF1KB3684
(Circular) (Six-base) MAPSORT of: pF1KB3684.seq Check: 2294 from: 1 to: 4904
pF1KB3684 4904 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1003 1583 1003
Size: 580 4324
AccI GT'mk_AC
Cuts at: 1111 1942 1111
Size: 831 4073
Acc65I G'GTAC_C
Cuts at: 1920 1920
Size: 4904
AclI AA'CG_TT
Cuts at: 4744 4744
Size: 4904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 772 1655 2729 3161 3908 4176 215
Size: 557 883 1074 432 747 268 943
Fragments arranged by size:
1074 943 883 747 557 432 268
AflIII A'CryG_T
Cuts at: 939 2256 3360 4337 939
Size: 1317 1104 977 1506
Fragments arranged by size:
1506 1317 1104 977
AgeI A'CCGG_T
Cuts at: 3292 3292
Size: 4904
AhdI GACnn_n'nnGTC
Cuts at: 525 525
Size: 4904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1246 1278 1246
Size: 32 4872
AlwNI CAG_nnn'CTG
Cuts at: 2209 3776 2209
Size: 1567 3337
ApaI G_GGCC'C
Cuts at: 513 1374 1873 513
Size: 861 499 3544
Fragments arranged by size:
3544 861 499
ApaLI G'TGCA_C
Cuts at: 3674 3674
Size: 4904
ApoI r'AATT_y
Cuts at: 1908 4257 4513 1908
Size: 2349 256 2299
Fragments arranged by size:
2349 2299 256
AseI AT'TA_AT
Cuts at: 20 374 20
Size: 354 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
AvaI C'yCGr_G
Cuts at: 1924 1924
Size: 4904
BamHI G'GATC_C
Cuts at: 1929 1929
Size: 4904
BanI G'GyrC_C
Cuts at: 452 721 1623 1920 2334 2582 2617 452
Size: 269 902 297 414 248 35 2739
Fragments arranged by size:
2739 902 414 297 269 248 35
BanII G_rGCy'C
Cuts at: 322 513 1313 1374 1565 1873 1918 2948 322
Size: 191 800 61 191 308 45 1030 2278
Fragments arranged by size:
2278 1030 800 308 191 191 61 45
BbeI G_GCGC'C
Cuts at: 456 2338 2586 456
Size: 1882 248 2774
Fragments arranged by size:
2774 1882 248
BbsI GAAGACnn'nnnn_
Cuts at: 1340 1340
Size: 4904
BciVI GTATCCnnnnn_n'
Cuts at: 2802 3563 4904 2802
Size: 761 1341 2802
Fragments arranged by size:
2802 1341 761
BclI T'GATC_A
Cuts at: 2424 2424
Size: 4904
BfrBI ATG'CAT
Cuts at: 1755 1755
Size: 4904
BglI GCCn_nnn'nGGC
Cuts at: 631 1301 631
Size: 670 4234
BglII A'GATC_T
Cuts at: 1616 2419 4456 1616
Size: 803 2037 2064
Fragments arranged by size:
2064 2037 803
BlpI GC'TnA_GC
Cuts at: 2016 2016
Size: 4904
Bme1580I G_kGCm'C
Cuts at: 513 609 879 1374 1481 1626 1873 2529 2622
Size: 96 270 495 107 145 247 656 93
Cuts at: 2622 3678 513
Size: 1056 1739
Fragments arranged by size:
1739 1056 656 495 270 247 145 107
96 93
BmrI ACTGGGnnnn_n'
Cuts at: 269 531 1368 2116 2197 2532 4676 269
Size: 262 837 748 81 335 2144 497
Fragments arranged by size:
2144 837 748 497 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 231 3271 231
Size: 3040 1864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 209
Size: 4904
Bpu10I CC'TnA_GC
Cuts at: 321 321
Size: 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1210 1579 2080 2959 3451 3749 694
Size: 516 369 501 879 492 298 1849
Fragments arranged by size:
1849 879 516 501 492 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 4608 4608
Size: 4904
BsaAI yAC'GTr
Cuts at: 2257 2257
Size: 4904
BsaBI GATnn'nnATC
Cuts at: 6 383 2418 4455 6
Size: 377 2035 2037 455
Fragments arranged by size:
2037 2035 455 377
BsaHI Gr'CG_yC
Cuts at: 453 1424 1773 2335 2583 3285 4781 453
Size: 971 349 562 248 702 1496 576
Fragments arranged by size:
1496 971 702 576 562 349 248
BsaWI w'CCGG_w
Cuts at: 2093 2614 3292 3566 3713 4124 2093
Size: 521 678 274 147 411 2873
Fragments arranged by size:
2873 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1532 1562 1532
Size: 30 4874
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 433 445 1344 1833 1876 1879 193
Size: 240 12 899 489 43 3 3218
Fragments arranged by size:
3218 899 489 240 43 12 3
BseYI C'CCAG_C
Cuts at: 594 947 1059 1837 2329 3087 3664 4160 594
Size: 353 112 778 492 758 577 496 1338
Fragments arranged by size:
1338 778 758 577 496 492 353 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 688 1903 667
Size: 21 1215 3668
Fragments arranged by size:
3668 1215 21
BsiEI CG_ry'CG
Cuts at: 104 2446 2492 3700 104
Size: 2342 46 1208 1308
Fragments arranged by size:
2342 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 322 1313 1918 2696 2886 3678 322
Size: 991 605 778 190 792 1548
Fragments arranged by size:
1548 991 792 778 605 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1764 1764
Size: 4904
Bsp1286I G_dGCh'C
Cuts at: 322 513 609 879 1313 1374 1481 1565 1626
Size: 191 96 270 434 61 107 84 61
Cuts at: 1626 1873 1918 2529 2622 2696 2886 2948 3678
Size: 247 45 611 93 74 190 62 730
Cuts at: 3678 322
Size: 1548
Fragments arranged by size:
1548 730 611 434 270 247 191 190
107 96 93 84 74 62 61 61
45
BspEI T'CCGG_A
Cuts at: 4124 4124
Size: 4904
BspHI T'CATG_A
Cuts at: 4080 4080
Size: 4904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 879 1003 1583 1954 2168 2470 2851 879
Size: 124 580 371 214 302 381 2932
Fragments arranged by size:
2932 580 381 371 302 214 124
BsrBI CCG'CTC
Cuts at: 50 401 507 588 1463 1692 3196 3250 4734
Size: 351 106 81 875 229 1504 54 1484
Cuts at: 4734 4899 50
Size: 165 55
Fragments arranged by size:
1504 1484 875 351 229 165 106 81
55 54
BsrDI GCAATG_nn'
Cuts at: 2816 2816
Size: 4904
BsrFI r'CCGG_y
Cuts at: 1781 2902 3083 3292 1781
Size: 1121 181 209 3393
Fragments arranged by size:
3393 1121 209 181
BsrGI T'GTAC_A
Cuts at: 459 1289 459
Size: 830 4074
BssSI C'ACGA_G
Cuts at: 3175 3533 3175
Size: 358 4546
BstAPI GCAn_nnn'nTGC
Cuts at: 1890 2281 1890
Size: 391 4513
BstBI TT'CG_AA
Cuts at: 3265 3265
Size: 4904
BstYI r'GATC_y
Cuts at: 1 1616 1929 2396 2419 2754 3000 4012 4098
Size: 1615 313 467 23 335 246 1012 86
Cuts at: 4098 4110 4456 1
Size: 12 346 449
Fragments arranged by size:
1615 1012 467 449 346 335 313 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1112 1112
Size: 4904
Bsu36I CC'TnA_GG
Cuts at: 216 216
Size: 4904
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 758 2854 3035 3198 758
Size: 2096 181 163 2464
Fragments arranged by size:
2464 2096 181 163
BtsI GCAGTG_nn'
Cuts at: 1157 1891 2147 1157
Size: 734 256 3914
Fragments arranged by size:
3914 734 256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 923 958 923
Size: 35 4869
DraI TTT'AAA
Cuts at: 1903 1903
Size: 4904
DraIII CAC_nnn'GTG
Cuts at: 920 1251 920
Size: 331 4573
DrdI GACnn_nn'nnGTC
Cuts at: 2610 3468 2610
Size: 858 4046
EaeI y'GGCC_r
Cuts at: 731 807 866 1316 2489 2663 3054 3081 4426
Size: 76 59 450 1173 174 391 27 1345
Cuts at: 4426 731
Size: 1209
Fragments arranged by size:
1345 1209 1173 450 391 174 76 59
27
EagI C'GGCC_G
Cuts at: 2489 2489
Size: 4904
EarI CTCTTCn'nnn_
Cuts at: 191 404 410 422 434 1351 2927 3137 191
Size: 213 6 12 12 917 1576 210 1958
Fragments arranged by size:
1958 1576 917 213 210 12 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 646 3112 3422 3568 4714 646
Size: 2466 310 146 1146 836
Fragments arranged by size:
2466 1146 836 310 146
EcoICRI GAG'CTC
Cuts at: 320 1311 1916 320
Size: 991 605 3308
Fragments arranged by size:
3308 991 605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 215 772 1655 2729 3161 3908 4176 209
Size: 6 557 883 1074 432 747 268 937
Fragments arranged by size:
1074 937 883 747 557 432 268 6
EcoO109I rG'GnC_Cy
Cuts at: 510 1370 1869 1870 2043 510
Size: 860 499 1 173 3371
Fragments arranged by size:
3371 860 499 173 1
EcoRI G'AATT_C
Cuts at: 1908 1908
Size: 4904
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 4904
FspI TGC'GCA
Cuts at: 2685 2685
Size: 4904
HaeII r_GCGC'y
Cuts at: 456 1788 2338 2586 3608 4587 456
Size: 1332 550 248 1022 979 773
Fragments arranged by size:
1332 1022 979 773 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 322 354 475 507 511 543 1486
Size: 32 121 32 121 32 4 32 943
Cuts at: 1486 1518 1761 1793 1807 1839 2746 2776 2778
Size: 32 243 32 14 32 907 30 2
Cuts at: 2778 2808 169
Size: 30 2265
Fragments arranged by size:
2265 943 907 243 121 121 32 32
32 32 32 32 32 30 30 14
4 2
HincII GTy'rAC
Cuts at: 1943 1943
Size: 4904
HindIII A'AGCT_T
Cuts at: 1637 1637
Size: 4904
KasI G'GCGC_C
Cuts at: 452 2334 2582 452
Size: 1882 248 2774
Fragments arranged by size:
2774 1882 248
KpnI G_GTAC'C
Cuts at: 1924 1924
Size: 4904
MluI A'CGCG_T
Cuts at: 4337 4337
Size: 4904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3575 3759 3575
Size: 184 4720
MscI TGG'CCA
Cuts at: 733 809 868 1318 2665 733
Size: 76 59 450 1347 2972
Fragments arranged by size:
2972 1347 450 76 59
MslI CAynn'nnrTG
Cuts at: 226 1364 2893 3020 226
Size: 1138 1529 127 2110
Fragments arranged by size:
2110 1529 1138 127
MspA1I CmG'CkG
Cuts at: 1633 2015 2329 2689 3702 3947 1633
Size: 382 314 360 1013 245 2590
Fragments arranged by size:
2590 1013 382 360 314 245
NaeI GCC'GGC
Cuts at: 3085 3085
Size: 4904
NarI GG'CG_CC
Cuts at: 453 2335 2583 453
Size: 1882 248 2774
Fragments arranged by size:
2774 1882 248
NgoMIV G'CCGG_C
Cuts at: 3083 3083
Size: 4904
NsiI A_TGCA'T
Cuts at: 1757 1757
Size: 4904
NspI r_CATG'y
Cuts at: 1740 1893 1957 2457 3364 1740
Size: 153 64 500 907 3280
Fragments arranged by size:
3280 907 500 153 64
PciI A'CATG_T
Cuts at: 3360 3360
Size: 4904
PflMI CCAn_nnn'nTGG
Cuts at: 972 972
Size: 4904
PmeI GTTT'AAAC
Cuts at: 1903 1903
Size: 4904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 4043
Size: 32 4872
PshAI GACnn'nnGTC
Cuts at: 359 359
Size: 4904
PspOMI G'GGCC_C
Cuts at: 509 1370 1869 509
Size: 861 499 3544
Fragments arranged by size:
3544 861 499
PstI C_TGCA'G
Cuts at: 665 1014 1951 2636 665
Size: 349 937 685 2933
Fragments arranged by size:
2933 937 685 349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4904
PvuII CAG'CTG
Cuts at: 1633 2329 2689 1633
Size: 696 360 3848
Fragments arranged by size:
3848 696 360
RsrII CG'GwC_CG
Cuts at: 3099 3099
Size: 4904
SacI G_AGCT'C
Cuts at: 322 1313 1918 322
Size: 991 605 3308
Fragments arranged by size:
3308 991 605
SalI G'TCGA_C
Cuts at: 1941 1941
Size: 4904
SapI GCTCTTCn'nnn_
Cuts at: 434 2927 3137 434
Size: 2493 210 2201
Fragments arranged by size:
2493 2201 210
SbfI CC_TGCA'GG
Cuts at: 1951 1951
Size: 4904
ScaI AGT'ACT
Cuts at: 791 1223 4421 791
Size: 432 3198 1274
Fragments arranged by size:
3198 1274 432
SexAI A'CCwGG_T
Cuts at: 1247 1247
Size: 4904
SfcI C'TryA_G
Cuts at: 33 661 1010 1947 2632 3625 3816 33
Size: 628 349 937 685 993 191 1121
Fragments arranged by size:
1121 993 937 685 628 349 191
SfoI GGC'GCC
Cuts at: 454 2336 2584 454
Size: 1882 248 2774
Fragments arranged by size:
2774 1882 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
SgrAI Cr'CCGG_yG
Cuts at: 1781 1781
Size: 4904
SmaI CCC'GGG
Cuts at: 1926 1926
Size: 4904
SmlI C'TyrA_G
Cuts at: 673 1225 1558 2059 2974 3466 3728 673
Size: 552 333 501 915 492 262 1849
Fragments arranged by size:
1849 915 552 501 492 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4904
SphI G_CATG'C
Cuts at: 1893 1957 2457 1893
Size: 64 500 4340
Fragments arranged by size:
4340 500 64
StuI AGG'CCT
Cuts at: 235 625 235
Size: 390 4514
StyI C'CwwG_G
Cuts at: 2038 2392 2038
Size: 354 4550
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 513 1144 1641 2487 2620 3079 3288 513
Size: 631 497 846 133 459 209 2129
Fragments arranged by size:
2129 846 631 497 459 209 133
TatI w'GTAC_w
Cuts at: 459 789 1221 1289 4419 459
Size: 330 432 68 3130 944
Fragments arranged by size:
3130 944 432 330 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4421 4453 4421
Size: 32 4872
Tth111I GACn'n_nGTC
Cuts at: 2701 2701
Size: 4904
XbaI T'CTAG_A
Cuts at: 1935 1935
Size: 4904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2399 2399
Size: 4904
XmaI C'CCGG_G
Cuts at: 1924 1924
Size: 4904
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AscI AvrII BaeI
BbvCI BcgI BmgBI BmtI BsiWI BsmI BssHII BstEII
BstXI BtgI ClaI EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PsiI PspXI PsrI SacII SanDI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI