Restriction map for
pF1KB3692
(Circular) (Six-base) MAPSORT of: pF1KB3692.seq Check: 4056 from: 1 to: 3497
pF1KB3692 3497 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 332 332
Size: 3497
AccI GT'mk_AC
Cuts at: 535 535
Size: 3497
Acc65I G'GTAC_C
Cuts at: 513 513
Size: 3497
AclI AA'CG_TT
Cuts at: 3337 3337
Size: 3497
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1754 2501 2769 1322
Size: 432 747 268 2050
Fragments arranged by size:
2050 747 432 268
AflIII A'CryG_T
Cuts at: 849 1953 2930 849
Size: 1104 977 1416
Fragments arranged by size:
1416 1104 977
AgeI A'CCGG_T
Cuts at: 1885 1885
Size: 3497
AlwNI CAG_nnn'CTG
Cuts at: 802 2369 802
Size: 1567 1930
ApaLI G'TGCA_C
Cuts at: 2267 2267
Size: 3497
ApoI r'AATT_y
Cuts at: 501 2850 3106 501
Size: 2349 256 892
Fragments arranged by size:
2349 892 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3497
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
AvaI C'yCGr_G
Cuts at: 517 517
Size: 3497
BamHI G'GATC_C
Cuts at: 522 522
Size: 3497
BanI G'GyrC_C
Cuts at: 513 927 1175 1210 513
Size: 414 248 35 2800
Fragments arranged by size:
2800 414 248 35
BanII G_rGCy'C
Cuts at: 511 1541 511
Size: 1030 2467
BbeI G_GCGC'C
Cuts at: 931 1179 931
Size: 248 3249
BbsI GAAGACnn'nnnn_
Cuts at: 153 153
Size: 3497
BciVI GTATCCnnnnn_n'
Cuts at: 1395 2156 3497 1395
Size: 761 1341 1395
Fragments arranged by size:
1395 1341 761
BclI T'GATC_A
Cuts at: 1017 1017
Size: 3497
BglII A'GATC_T
Cuts at: 1012 3049 1012
Size: 2037 1460
BlpI GC'TnA_GC
Cuts at: 609 609
Size: 3497
Bme1580I G_kGCm'C
Cuts at: 1122 1215 2271 1122
Size: 93 1056 2348
Fragments arranged by size:
2348 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 709 790 1125 3269 709
Size: 81 335 2144 937
Fragments arranged by size:
2144 937 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 498
Size: 3497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 673 1552 2044 2342 363
Size: 310 879 492 298 1518
Fragments arranged by size:
1518 879 492 310 298
BsaI GGTCTCn'nnnn_
Cuts at: 3201 3201
Size: 3497
BsaAI yAC'GTr
Cuts at: 850 850
Size: 3497
BsaBI GATnn'nnATC
Cuts at: 6 1011 3048 6
Size: 1005 2037 455
Fragments arranged by size:
2037 1005 455
BsaHI Gr'CG_yC
Cuts at: 329 928 1176 1878 3374 329
Size: 599 248 702 1496 452
Fragments arranged by size:
1496 702 599 452 248
BsaWI w'CCGG_w
Cuts at: 686 1207 1885 2159 2306 2717 686
Size: 521 678 274 147 411 1466
Fragments arranged by size:
1466 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 113 143 113
Size: 30 3467
BseYI C'CCAG_C
Cuts at: 274 922 1680 2257 2753 274
Size: 648 758 577 496 1018
Fragments arranged by size:
1018 758 648 577 496
BsiEI CG_ry'CG
Cuts at: 104 1039 1085 2293 104
Size: 935 46 1208 1308
Fragments arranged by size:
1308 1208 935 46
BsiHKAI G_wGCw'C
Cuts at: 511 1289 1479 2271 511
Size: 778 190 792 1737
Fragments arranged by size:
1737 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 511 1122 1215 1289 1479 1541 2271 511
Size: 611 93 74 190 62 730 1737
Fragments arranged by size:
1737 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2717 2717
Size: 3497
BspHI T'CATG_A
Cuts at: 2673 2673
Size: 3497
BspMI ACCTGCnnnn'nnnn_
Cuts at: 547 761 1063 1444 547
Size: 214 302 381 2600
Fragments arranged by size:
2600 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1789 1843 3327 3492 50
Size: 1739 54 1484 165 55
Fragments arranged by size:
1739 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 312 1409 312
Size: 1097 2400
BsrFI r'CCGG_y
Cuts at: 1495 1676 1885 1495
Size: 181 209 3107
Fragments arranged by size:
3107 209 181
BssSI C'ACGA_G
Cuts at: 1768 2126 1768
Size: 358 3139
BstAPI GCAn_nnn'nTGC
Cuts at: 874 874
Size: 3497
BstBI TT'CG_AA
Cuts at: 1858 1858
Size: 3497
BstYI r'GATC_y
Cuts at: 1 522 989 1012 1347 1593 2605 2691 2703
Size: 521 467 23 335 246 1012 86 12
Cuts at: 2703 3049 1
Size: 346 449
Fragments arranged by size:
1012 521 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1447 1628 1791 1447
Size: 181 163 3153
Fragments arranged by size:
3153 181 163
BtsI GCAGTG_nn'
Cuts at: 193 740 193
Size: 547 2950
DraI TTT'AAA
Cuts at: 496 496
Size: 3497
DrdI GACnn_nn'nnGTC
Cuts at: 1203 2061 1203
Size: 858 2639
EaeI y'GGCC_r
Cuts at: 1082 1256 1647 1674 3019 1082
Size: 174 391 27 1345 1560
Fragments arranged by size:
1560 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1082 1082
Size: 3497
EarI CTCTTCn'nnn_
Cuts at: 1520 1730 1520
Size: 210 3287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1705 2015 2161 3307 1705
Size: 310 146 1146 1895
Fragments arranged by size:
1895 1146 310 146
EcoICRI GAG'CTC
Cuts at: 509 509
Size: 3497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 1322 1754 2501 2769 498
Size: 824 432 747 268 1226
Fragments arranged by size:
1226 824 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 381 636 381
Size: 255 3242
EcoRI G'AATT_C
Cuts at: 501 501
Size: 3497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1516 1516
Size: 3497
FspI TGC'GCA
Cuts at: 1278 1278
Size: 3497
HaeII r_GCGC'y
Cuts at: 931 1179 2201 3180 931
Size: 248 1022 979 1248
Fragments arranged by size:
1248 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 471 503 1339 1369 1371 1401 113
Size: 32 326 32 836 30 2 30 2209
Fragments arranged by size:
2209 836 326 32 32 30 30 2
HincII GTy'rAC
Cuts at: 536 536
Size: 3497
KasI G'GCGC_C
Cuts at: 927 1175 927
Size: 248 3249
KpnI G_GTAC'C
Cuts at: 517 517
Size: 3497
MfeI C'AATT_G
Cuts at: 234 234
Size: 3497
MluI A'CGCG_T
Cuts at: 2930 2930
Size: 3497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 280 2168 2352 280
Size: 1888 184 1425
Fragments arranged by size:
1888 1425 184
MscI TGG'CCA
Cuts at: 1258 1258
Size: 3497
MslI CAynn'nnrTG
Cuts at: 1486 1613 1486
Size: 127 3370
MspA1I CmG'CkG
Cuts at: 608 922 1282 2295 2540 608
Size: 314 360 1013 245 1565
Fragments arranged by size:
1565 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1678 1678
Size: 3497
NarI GG'CG_CC
Cuts at: 928 1176 928
Size: 248 3249
NcoI C'CATG_G
Cuts at: 107 107
Size: 3497
NgoMIV G'CCGG_C
Cuts at: 1676 1676
Size: 3497
NspI r_CATG'y
Cuts at: 550 1050 1957 550
Size: 500 907 2090
Fragments arranged by size:
2090 907 500
PciI A'CATG_T
Cuts at: 1953 1953
Size: 3497
PmeI GTTT'AAAC
Cuts at: 496 496
Size: 3497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2636 2668 2636
Size: 32 3465
PpuMI rG'GwC_Cy
Cuts at: 381 381
Size: 3497
PstI C_TGCA'G
Cuts at: 188 544 1229 188
Size: 356 685 2456
Fragments arranged by size:
2456 685 356
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3497
PvuII CAG'CTG
Cuts at: 922 1282 922
Size: 360 3137
RsrII CG'GwC_CG
Cuts at: 1692 1692
Size: 3497
SacI G_AGCT'C
Cuts at: 511 511
Size: 3497
SalI G'TCGA_C
Cuts at: 534 534
Size: 3497
SapI GCTCTTCn'nnn_
Cuts at: 1520 1730 1520
Size: 210 3287
SbfI CC_TGCA'GG
Cuts at: 544 544
Size: 3497
ScaI AGT'ACT
Cuts at: 3014 3014
Size: 3497
SfcI C'TryA_G
Cuts at: 33 184 540 1225 2218 2409 33
Size: 151 356 685 993 191 1121
Fragments arranged by size:
1121 993 685 356 191 151
SfoI GGC'GCC
Cuts at: 929 1177 929
Size: 248 3249
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
SmaI CCC'GGG
Cuts at: 519 519
Size: 3497
SmlI C'TyrA_G
Cuts at: 342 652 1567 2059 2321 342
Size: 310 915 492 262 1518
Fragments arranged by size:
1518 915 492 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3497
SphI G_CATG'C
Cuts at: 550 1050 550
Size: 500 2997
StyI C'CwwG_G
Cuts at: 107 117 631 985 107
Size: 10 514 354 2619
Fragments arranged by size:
2619 514 354 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1080 1213 1672 1881 1080
Size: 133 459 209 2696
Fragments arranged by size:
2696 459 209 133
TatI w'GTAC_w
Cuts at: 3012 3012
Size: 3497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3465
Tth111I GACn'n_nGTC
Cuts at: 1294 1294
Size: 3497
XbaI T'CTAG_A
Cuts at: 528 528
Size: 3497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 992 992
Size: 3497
XmaI C'CCGG_G
Cuts at: 517 517
Size: 3497
ZraI GAC'GTC
Cuts at: 330 330
Size: 3497
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI