Restriction map for
pF1KB3698
(Circular) (Six-base) MAPSORT of: pF1KB3698.seq Check: 6159 from: 1 to: 4238
pF1KB3698 4238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 707 707
Size: 4238
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1083 1195 2063 2495 3242 3510 1083
Size: 112 868 432 747 268 1811
Fragments arranged by size:
1811 868 747 432 268 112
AflIII A'CryG_T
Cuts at: 527 1590 2694 3671 527
Size: 1063 1104 977 1094
Fragments arranged by size:
1104 1094 1063 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AleI CACnn'nnGTG
Cuts at: 676 691 676
Size: 15 4223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1201 1233 1201
Size: 32 4206
AlwNI CAG_nnn'CTG
Cuts at: 1543 3110 1543
Size: 1567 2671
ApaLI G'TGCA_C
Cuts at: 495 694 949 3008 495
Size: 199 255 2059 1725
Fragments arranged by size:
2059 1725 255 199
ApoI r'AATT_y
Cuts at: 1137 1242 3591 3847 1137
Size: 105 2349 256 1528
Fragments arranged by size:
2349 1528 256 105
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 128 193 757 1087 1258 128
Size: 65 564 330 171 3108
Fragments arranged by size:
3108 564 330 171 65
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 157 223 678 845 1254 1668 1916 1951 157
Size: 66 455 167 409 414 248 35 2444
Fragments arranged by size:
2444 455 414 409 248 167 66 35
BanII G_rGCy'C
Cuts at: 200 254 1252 2282 200
Size: 54 998 1030 2156
Fragments arranged by size:
2156 1030 998 54
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbsI GAAGACnn'nnnn_
Cuts at: 534 900 930 534
Size: 366 30 3842
Fragments arranged by size:
3842 366 30
BbvCI CC'TCA_GC
Cuts at: 1111 1111
Size: 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 873 907 873
Size: 34 4204
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglI GCCn_nnn'nGGC
Cuts at: 867 1177 867
Size: 310 3928
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 634 1350 634
Size: 716 3522
Bme1580I G_kGCm'C
Cuts at: 226 499 683 698 785 953 1863 1956 3012
Size: 273 184 15 87 168 910 93 1056
Cuts at: 3012 226
Size: 1452
Fragments arranged by size:
1452 1056 910 273 184 168 93 87
15
BmrI ACTGGGnnnn_n'
Cuts at: 1450 1531 1866 4010 1450
Size: 81 335 2144 1678
Fragments arranged by size:
2144 1678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 163
Size: 4238
Bpu10I CC'TnA_GC
Cuts at: 1111 1111
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1087 3942 1087
Size: 2855 1383
BsaAI yAC'GTr
Cuts at: 273 1591 273
Size: 1318 2920
BsaBI GATnn'nnATC
Cuts at: 6 382 1752 3789 6
Size: 376 1370 2037 455
Fragments arranged by size:
2037 1370 455 376
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 202 736 766 172
Size: 30 534 30 3644
Fragments arranged by size:
3644 534 30 30
BseYI C'CCAG_C
Cuts at: 768 1663 2421 2998 3494 768
Size: 895 758 577 496 1512
Fragments arranged by size:
1512 895 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 769 448
Size: 321 3917
BsiEI CG_ry'CG
Cuts at: 104 570 1780 1826 3034 104
Size: 466 1210 46 1208 1308
Fragments arranged by size:
1308 1210 1208 466 46
BsiHKAI G_wGCw'C
Cuts at: 200 254 263 499 698 953 1252 2030 2220
Size: 54 9 236 199 255 299 778 190
Cuts at: 2220 3012 200
Size: 792 1426
Fragments arranged by size:
1426 792 778 299 255 236 199 190
54 9
Bsp1286I G_dGCh'C
Cuts at: 200 226 254 263 499 683 698 785 953
Size: 26 28 9 236 184 15 87 168
Cuts at: 953 1252 1863 1956 2030 2220 2282 3012 200
Size: 299 611 93 74 190 62 730 1426
Fragments arranged by size:
1426 730 611 299 236 190 184 168
93 87 74 62 28 26 15 9
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 470 510 707 1288 1502 1804 2185 470
Size: 40 197 581 214 302 381 2523
Fragments arranged by size:
2523 581 381 302 214 197 40
BsrBI CCG'CTC
Cuts at: 50 216 471 982 2530 2584 4068 4233 50
Size: 166 255 511 1548 54 1484 165 55
Fragments arranged by size:
1548 1484 511 255 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2150 2150
Size: 4238
BsrFI r'CCGG_y
Cuts at: 842 1143 2236 2417 2626 842
Size: 301 1093 181 209 2454
Fragments arranged by size:
2454 1093 301 209 181
BsrGI T'GTAC_A
Cuts at: 530 1118 530
Size: 588 3650
BssSI C'ACGA_G
Cuts at: 291 466 2509 2867 291
Size: 175 2043 358 1662
Fragments arranged by size:
2043 1662 358 175
BstAPI GCAn_nnn'nTGC
Cuts at: 957 1615 957
Size: 658 3580
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstYI r'GATC_y
Cuts at: 1 1263 1730 1753 2088 2334 3346 3432 3444
Size: 1262 467 23 335 246 1012 86 12
Cuts at: 3444 3790 1
Size: 346 449
Fragments arranged by size:
1262 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 849 1229 107
Size: 742 380 3116
Fragments arranged by size:
3116 742 380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 468 561 2188 2369 2532 468
Size: 93 1627 181 163 2174
Fragments arranged by size:
2174 1627 181 163 93
BtsI GCAGTG_nn'
Cuts at: 757 1230 1481 757
Size: 473 251 3514
Fragments arranged by size:
3514 473 251
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 487 1944 2802 487
Size: 1457 858 1923
Fragments arranged by size:
1923 1457 858
EaeI y'GGCC_r
Cuts at: 233 434 584 1823 1997 2388 2415 3760 233
Size: 201 150 1239 174 391 27 1345 711
Fragments arranged by size:
1345 1239 711 391 201 174 150 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 319 517 2261 2471 319
Size: 198 1744 210 2086
Fragments arranged by size:
2086 1744 210 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 433 991 2446 2756 2902 4048 433
Size: 558 1455 310 146 1146 623
Fragments arranged by size:
1455 1146 623 558 310 146
EcoICRI GAG'CTC
Cuts at: 198 252 1250 198
Size: 54 998 3186
Fragments arranged by size:
3186 998 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1083 1195 2063 2495 3242 3510 163
Size: 920 112 868 432 747 268 891
Fragments arranged by size:
920 891 868 747 432 268 112
EcoNI CCTnn'n_nnAGG
Cuts at: 448 448
Size: 4238
EcoO109I rG'GnC_Cy
Cuts at: 1377 1377
Size: 4238
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 880 1672 1920 2942 3921 880
Size: 792 248 1022 979 1197
Fragments arranged by size:
1197 1022 979 792 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2080 2110 2112 2142 2080
Size: 30 2 30 4176
Fragments arranged by size:
4176 30 30 2
HincII GTy'rAC
Cuts at: 525 1035 1277 525
Size: 510 242 3486
Fragments arranged by size:
3486 510 242
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1076 1187 2909 3093 1076
Size: 111 1722 184 2221
Fragments arranged by size:
2221 1722 184 111
MscI TGG'CCA
Cuts at: 235 586 1999 235
Size: 351 1413 2474
Fragments arranged by size:
2474 1413 351
MslI CAynn'nnrTG
Cuts at: 112 430 451 502 556 652 676 691 946
Size: 318 21 51 54 96 24 15 255
Cuts at: 946 2227 2354 112
Size: 1281 127 1996
Fragments arranged by size:
1996 1281 318 255 127 96 54 51
24 21 15
MspA1I CmG'CkG
Cuts at: 963 1058 1349 1663 2023 3036 3281 963
Size: 95 291 314 360 1013 245 1920
Fragments arranged by size:
1920 1013 360 314 291 245 95
NaeI GCC'GGC
Cuts at: 1145 2419 1145
Size: 1274 2964
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NcoI C'CATG_G
Cuts at: 107 107
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 1143 2417 1143
Size: 1274 2964
NspI r_CATG'y
Cuts at: 531 1291 1791 2698 531
Size: 760 500 907 2071
Fragments arranged by size:
2071 907 760 500
PciI A'CATG_T
Cuts at: 527 2694 527
Size: 2167 2071
PfoI T'CCnGG_A
Cuts at: 740 740
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PspXI vC'TCGA_Gb
Cuts at: 757 757
Size: 4238
PstI C_TGCA'G
Cuts at: 521 604 704 962 1285 1970 521
Size: 83 100 258 323 685 2789
Fragments arranged by size:
2789 685 323 258 100 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1058 1663 2023 1058
Size: 605 360 3273
Fragments arranged by size:
3273 605 360
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 200 254 1252 200
Size: 54 998 3186
Fragments arranged by size:
3186 998 54
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 517 2261 2471 517
Size: 1744 210 2284
Fragments arranged by size:
2284 1744 210
SbfI CC_TGCA'GG
Cuts at: 521 1285 521
Size: 764 3474
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 517 600 700 958 1077 1281 1966 2959
Size: 484 83 100 258 119 204 685 993
Cuts at: 2959 3150 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 484 258 204 191 119
100 83
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 130 1260 130
Size: 1130 3108
SmlI C'TyrA_G
Cuts at: 757 1393 2308 2800 3062 757
Size: 636 915 492 262 1933
Fragments arranged by size:
1933 915 636 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StuI AGG'CCT
Cuts at: 718 718
Size: 4238
StyI C'CwwG_G
Cuts at: 107 1372 1726 107
Size: 1265 354 2619
Fragments arranged by size:
2619 1265 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 1954 2413 2622 1821
Size: 133 459 209 3437
Fragments arranged by size:
3437 459 209 133
TatI w'GTAC_w
Cuts at: 395 530 1118 3753 395
Size: 135 588 2635 880
Fragments arranged by size:
2635 880 588 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 736 768 3755 3787 736
Size: 32 2987 32 1187
Fragments arranged by size:
2987 1187 32 32
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 1733 327
Size: 1406 2832
XhoI C'TCGA_G
Cuts at: 757 757
Size: 4238
XmaI C'CCGG_G
Cuts at: 128 1258 128
Size: 1130 3108
XmnI GAAnn'nnTTC
Cuts at: 937 1156 937
Size: 219 4019
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BseRI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI