Restriction map for
pF1KB3700
(Circular) (Six-base) MAPSORT of: pF1KB3700.seq Check: 1573 from: 1 to: 5033
pF1KB3700 5033 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1633 1633
Size: 5033
AccI GT'mk_AC
Cuts at: 1342 2071 1342
Size: 729 4304
Acc65I G'GTAC_C
Cuts at: 2049 2049
Size: 5033
AclI AA'CG_TT
Cuts at: 4873 4873
Size: 5033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 2858 3290 3643 4305 1888
Size: 970 432 353 662 2616
Fragments arranged by size:
2616 970 662 432 353
AflIII A'CryG_T
Cuts at: 1094 1671 2385 4185 4466 1094
Size: 577 714 1800 281 1661
Fragments arranged by size:
1800 1661 714 577 281
AleI CACnn'nnGTG
Cuts at: 880 1576 880
Size: 696 4337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 5033
AlwNI CAG_nnn'CTG
Cuts at: 636 820 1926 1984 2338 3776 636
Size: 184 1106 58 354 1438 1893
Fragments arranged by size:
1893 1438 1106 354 184 58
ApaI G_GGCC'C
Cuts at: 248 989 248
Size: 741 4292
ApaLI G'TGCA_C
Cuts at: 769 1891 1993 3871 769
Size: 1122 102 1878 1931
Fragments arranged by size:
1931 1878 1122 102
ApoI r'AATT_y
Cuts at: 1876 2037 4386 4642 1876
Size: 161 2349 256 2267
Fragments arranged by size:
2349 2267 256 161
AseI AT'TA_AT
Cuts at: 20 20
Size: 5033
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
AvaI C'yCGr_G
Cuts at: 383 1498 2053 383
Size: 1115 555 3363
Fragments arranged by size:
3363 1115 555
BamHI G'GATC_C
Cuts at: 2058 2058
Size: 5033
BanI G'GyrC_C
Cuts at: 1189 1774 2049 2463 2711 2746 1189
Size: 585 275 414 248 35 3476
Fragments arranged by size:
3476 585 414 275 248 35
BanII G_rGCy'C
Cuts at: 248 695 989 1616 1636 2047 3077 248
Size: 447 294 627 20 411 1030 2204
Fragments arranged by size:
2204 1030 627 447 411 294 20
BbeI G_GCGC'C
Cuts at: 1193 2467 2715 1193
Size: 1274 248 3511
Fragments arranged by size:
3511 1274 248
BbsI GAAGACnn'nnnn_
Cuts at: 1470 1560 1470
Size: 90 4943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 350 384 350
Size: 34 4999
BciVI GTATCCnnnnn_n'
Cuts at: 2931 3987 5033 2931
Size: 1056 1046 2931
Fragments arranged by size:
2931 1056 1046
BclI T'GATC_A
Cuts at: 1133 1727 2553 1133
Size: 594 826 3613
Fragments arranged by size:
3613 826 594
BglI GCCn_nnn'nGGC
Cuts at: 341 616 782 838 1355 1547 341
Size: 275 166 56 517 192 3827
Fragments arranged by size:
3827 517 275 192 166 56
BglII A'GATC_T
Cuts at: 2548 4585 2548
Size: 2037 2996
BlpI GC'TnA_GC
Cuts at: 1825 2145 1825
Size: 320 4713
Bme1580I G_kGCm'C
Cuts at: 238 248 773 989 1583 1777 1895 1997 2658
Size: 10 525 216 594 194 118 102 661
Cuts at: 2658 2751 3875 238
Size: 93 1124 1396
Fragments arranged by size:
1396 1124 661 594 525 216 194 118
102 93 10
BmgBI CAC'GTC
Cuts at: 507 1095 1166 507
Size: 588 71 4374
Fragments arranged by size:
4374 588 71
BmrI ACTGGGnnnn_n'
Cuts at: 2245 2326 2661 4805 2245
Size: 81 335 2144 2473
Fragments arranged by size:
2473 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 5033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1011 1650 1903 1011
Size: 639 253 4141
Fragments arranged by size:
4141 639 253
Bpu10I CC'TnA_GC
Cuts at: 1150 1150
Size: 5033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1678 2209 3088 3802 4100 1678
Size: 531 879 714 298 2611
Fragments arranged by size:
2611 879 714 531 298
BsaI GGTCTCn'nnnn_
Cuts at: 4737 4737
Size: 5033
BsaAI yAC'GTr
Cuts at: 2386 2386
Size: 5033
BsaBI GATnn'nnATC
Cuts at: 6 1318 2547 4584 6
Size: 1312 1229 2037 455
Fragments arranged by size:
2037 1312 1229 455
BsaHI Gr'CG_yC
Cuts at: 1190 2464 2712 3414 4910 1190
Size: 1274 248 702 1496 1313
Fragments arranged by size:
1496 1313 1274 702 248
BsaWI w'CCGG_w
Cuts at: 2222 2743 3421 3832 3979 4253 2222
Size: 521 678 411 147 274 3002
Fragments arranged by size:
3002 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 379 409 544 550 574 580 1736 1766 379
Size: 30 135 6 24 6 1156 30 3646
Fragments arranged by size:
3646 1156 135 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1021 1186 1417 1021
Size: 165 231 4637
Fragments arranged by size:
4637 231 165
BseYI C'CCAG_C
Cuts at: 1652 1966 2458 3216 3881 4289 1652
Size: 314 492 758 665 408 2396
Fragments arranged by size:
2396 758 665 492 408 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1078 1786 310
Size: 768 708 3557
Fragments arranged by size:
3557 768 708
BsiEI CG_ry'CG
Cuts at: 104 2575 2621 3851 104
Size: 2471 46 1230 1286
Fragments arranged by size:
2471 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 185 773 1636 1895 1997 2047 2825 3015 3875
Size: 588 863 259 102 50 778 190 860
Cuts at: 3875 185
Size: 1343
Fragments arranged by size:
1343 863 860 778 588 259 190 102
50
Bsp1286I G_dGCh'C
Cuts at: 185 238 248 695 773 989 1583 1616 1636
Size: 53 10 447 78 216 594 33 20
Cuts at: 1636 1777 1895 1997 2047 2658 2751 2825 3015
Size: 141 118 102 50 611 93 74 190
Cuts at: 3015 3077 3875 185
Size: 62 798 1343
Fragments arranged by size:
1343 798 611 594 447 216 190 141
118 102 93 78 74 62 53 50
33 20 10
BspHI T'CATG_A
Cuts at: 3465 3465
Size: 5033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 1633 1982 2083 2297 2599 2980 332
Size: 1301 349 101 214 302 381 2385
Fragments arranged by size:
2385 1301 381 349 302 214 101
BsrBI CCG'CTC
Cuts at: 50 972 1035 1757 3325 3379 4863 5028 50
Size: 922 63 722 1568 54 1484 165 55
Fragments arranged by size:
1568 1484 922 722 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 579 2945 579
Size: 2366 2667
BsrFI r'CCGG_y
Cuts at: 167 521 1100 1447 1958 3031 3212 167
Size: 354 579 347 511 1073 181 1988
Fragments arranged by size:
1988 1073 579 511 354 347 181
BsrGI T'GTAC_A
Cuts at: 1842 1842
Size: 5033
BssSI C'ACGA_G
Cuts at: 114 3304 4012 114
Size: 3190 708 1135
Fragments arranged by size:
3190 1135 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1431 2410 1431
Size: 979 4054
BstBI TT'CG_AA
Cuts at: 1283 3394 1283
Size: 2111 2922
BstEII G'GTnAC_C
Cuts at: 1659 1659
Size: 5033
BstYI r'GATC_y
Cuts at: 1 2058 2525 2548 2883 3129 3435 3447 3533
Size: 2057 467 23 335 246 306 12 86
Cuts at: 3533 4585 1
Size: 1052 449
Fragments arranged by size:
2057 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 154 154
Size: 5033
BtgI C'CryG_G
Cuts at: 903 1224 903
Size: 321 4712
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2983 3164 3327 2983
Size: 181 163 4689
Fragments arranged by size:
4689 181 163
BtsI GCAGTG_nn'
Cuts at: 815 2276 815
Size: 1461 3572
ClaI AT'CG_AT
Cuts at: 515 515
Size: 5033
DraI TTT'AAA
Cuts at: 2032 2032
Size: 5033
DraIII CAC_nnn'GTG
Cuts at: 1863 1863
Size: 5033
DrdI GACnn_nn'nnGTC
Cuts at: 2739 4083 2739
Size: 1344 3689
EaeI y'GGCC_r
Cuts at: 1330 1373 1988 2618 2792 3183 3210 4555 1330
Size: 43 615 630 174 391 27 1345 1808
Fragments arranged by size:
1808 1345 630 615 391 174 43 27
EagI C'GGCC_G
Cuts at: 2618 2618
Size: 5033
EarI CTCTTCn'nnn_
Cuts at: 190 205 3056 3266 190
Size: 15 2851 210 1957
Fragments arranged by size:
2851 1957 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 937 3241 3983 4129 4843 937
Size: 2304 742 146 714 1127
Fragments arranged by size:
2304 1127 742 714 146
EcoICRI GAG'CTC
Cuts at: 1634 2045 1634
Size: 411 4622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1011 1650 1888 1903 2858 3290 3643 4305 1011
Size: 639 238 15 955 432 353 662 1739
Fragments arranged by size:
1739 955 662 639 432 353 238 15
EcoNI CCTnn'n_nnAGG
Cuts at: 328 706 853 328
Size: 378 147 4508
Fragments arranged by size:
4508 378 147
EcoO109I rG'GnC_Cy
Cuts at: 192 244 285 435 633 986 1209 1923 2172
Size: 52 41 150 198 353 223 714 249
Cuts at: 2172 192
Size: 3053
Fragments arranged by size:
3053 714 353 249 223 198 150 52
41
EcoRI G'AATT_C
Cuts at: 2037 2037
Size: 5033
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 201 1272 3052 201
Size: 1071 1780 2182
Fragments arranged by size:
2182 1780 1071
FspI TGC'GCA
Cuts at: 886 955 1486 2814 886
Size: 69 531 1328 3105
Fragments arranged by size:
3105 1328 531 69
HaeII r_GCGC'y
Cuts at: 489 1193 2467 2715 3945 4716 489
Size: 704 1274 248 1230 771 806
Fragments arranged by size:
1274 1230 806 771 704 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1599 1736 1768 2875 2905 2907 2937 1567
Size: 32 137 32 1107 30 2 30 3663
Fragments arranged by size:
3663 1107 137 32 32 30 30 2
HincII GTy'rAC
Cuts at: 537 2072 537
Size: 1535 3498
KasI G'GCGC_C
Cuts at: 1189 2463 2711 1189
Size: 1274 248 3511
Fragments arranged by size:
3511 1274 248
KpnI G_GTAC'C
Cuts at: 2053 2053
Size: 5033
MluI A'CGCG_T
Cuts at: 4466 4466
Size: 5033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 587 917 1610 3792 3976 587
Size: 330 693 2182 184 1644
Fragments arranged by size:
2182 1644 693 330 184
MscI TGG'CCA
Cuts at: 1375 2794 1375
Size: 1419 3614
MslI CAynn'nnrTG
Cuts at: 880 1576 3022 3149 880
Size: 696 1446 127 2764
Fragments arranged by size:
2764 1446 696 127
MspA1I CmG'CkG
Cuts at: 820 1226 1329 1656 2144 2458 2818 3602 3847
Size: 406 103 327 488 314 360 784 245
Cuts at: 3847 820
Size: 2006
Fragments arranged by size:
2006 784 488 406 360 327 314 245
103
NaeI GCC'GGC
Cuts at: 169 1449 1960 3214 169
Size: 1280 511 1254 1988
Fragments arranged by size:
1988 1280 1254 511
NarI GG'CG_CC
Cuts at: 1190 2464 2712 1190
Size: 1274 248 3511
Fragments arranged by size:
3511 1274 248
NgoMIV G'CCGG_C
Cuts at: 167 1447 1958 3212 167
Size: 1280 511 1254 1988
Fragments arranged by size:
1988 1280 1254 511
NspI r_CATG'y
Cuts at: 762 1425 1675 2086 2586 4189 762
Size: 663 250 411 500 1603 1606
Fragments arranged by size:
1606 1603 663 500 411 250
PasI CC'CwG_GG
Cuts at: 962 962
Size: 5033
PciI A'CATG_T
Cuts at: 1671 4185 1671
Size: 2514 2519
PflMI CCAn_nnn'nTGG
Cuts at: 636 636
Size: 5033
PfoI T'CCnGG_A
Cuts at: 1943 1943
Size: 5033
PmeI GTTT'AAAC
Cuts at: 2032 2032
Size: 5033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 5001
PpuMI rG'GwC_Cy
Cuts at: 192 285 435 1209 192
Size: 93 150 774 4016
Fragments arranged by size:
4016 774 150 93
PspOMI G'GGCC_C
Cuts at: 244 985 244
Size: 741 4292
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1258 1290 1399 1431 1258
Size: 32 109 32 4860
Fragments arranged by size:
4860 109 32 32
PstI C_TGCA'G
Cuts at: 1436 2080 2765 1436
Size: 644 685 3704
Fragments arranged by size:
3704 685 644
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5033
PvuII CAG'CTG
Cuts at: 820 1656 2458 2818 820
Size: 836 802 360 3035
Fragments arranged by size:
3035 836 802 360
RsrII CG'GwC_CG
Cuts at: 3228 3228
Size: 5033
SacI G_AGCT'C
Cuts at: 1636 2047 1636
Size: 411 4622
SacII CC_GC'GG
Cuts at: 1227 1227
Size: 5033
SalI G'TCGA_C
Cuts at: 2070 2070
Size: 5033
SanDI GG'GwC_CC
Cuts at: 435 435
Size: 5033
SapI GCTCTTCn'nnn_
Cuts at: 190 3056 3266 190
Size: 2866 210 1957
Fragments arranged by size:
2866 1957 210
SbfI CC_TGCA'GG
Cuts at: 2080 2080
Size: 5033
ScaI AGT'ACT
Cuts at: 4550 4550
Size: 5033
SfcI C'TryA_G
Cuts at: 33 1432 2076 2761 3729 3920 33
Size: 1399 644 685 968 191 1146
Fragments arranged by size:
1399 1146 968 685 644 191
SfoI GGC'GCC
Cuts at: 1191 2465 2713 1191
Size: 1274 248 3511
Fragments arranged by size:
3511 1274 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
SmaI CCC'GGG
Cuts at: 385 2055 385
Size: 1670 3363
SmlI C'TyrA_G
Cuts at: 1693 2188 3103 3817 4079 1693
Size: 495 915 714 262 2647
Fragments arranged by size:
2647 915 714 495 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5033
SphI G_CATG'C
Cuts at: 1425 2086 2586 1425
Size: 661 500 3872
Fragments arranged by size:
3872 661 500
StuI AGG'CCT
Cuts at: 604 604
Size: 5033
StyI C'CwwG_G
Cuts at: 269 2167 2521 269
Size: 1898 354 2781
Fragments arranged by size:
2781 1898 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 489 721 1036 1964 2616 2749 3208 3417 489
Size: 232 315 928 652 133 459 209 2105
Fragments arranged by size:
2105 928 652 459 315 232 209 133
TatI w'GTAC_w
Cuts at: 1274 1790 1842 4548 1274
Size: 516 52 2706 1759
Fragments arranged by size:
2706 1759 516 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 550 582 4550 4582 379
Size: 32 139 32 3968 32 830
Fragments arranged by size:
3968 830 139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 508 1750 2830 508
Size: 1242 1080 2711
Fragments arranged by size:
2711 1242 1080
XbaI T'CTAG_A
Cuts at: 2064 2064
Size: 5033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 637 1625 2528 637
Size: 988 903 3142
Fragments arranged by size:
3142 988 903
XmaI C'CCGG_G
Cuts at: 383 2053 383
Size: 1670 3363
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmI BsmBI BspEI
BssHII BstXI BstZ17I CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI