Restriction map for
pF1KB3701
(Circular) (Six-base) MAPSORT of: pF1KB3701.seq Check: 5090 from: 1 to: 6647
pF1KB3701 6647 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1086 1086
Size: 6647
AatII G_ACGT'C
Cuts at: 1949 1949
Size: 6647
AccI GT'mk_AC
Cuts at: 1133 3092 3685 1133
Size: 1959 593 4095
Fragments arranged by size:
4095 1959 593
Acc65I G'GTAC_C
Cuts at: 3663 3663
Size: 6647
AclI AA'CG_TT
Cuts at: 6487 6487
Size: 6647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1800 1884 1896 2368 3031 4472 4904 5257
Size: 1667 84 12 472 663 1441 432 353
Cuts at: 5257 5919 133
Size: 662 861
Fragments arranged by size:
1667 1441 861 663 662 472 432 353
84 12
AflIII A'CryG_T
Cuts at: 2429 2687 3999 5799 6080 2429
Size: 258 1312 1800 281 2996
Fragments arranged by size:
2996 1800 1312 281 258
AleI CACnn'nnGTG
Cuts at: 1849 2581 1849
Size: 732 5915
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3295 3295
Size: 6647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1528 1560 1528
Size: 32 6615
AlwNI CAG_nnn'CTG
Cuts at: 1521 1531 1836 2031 2808 3075 3516 3952 5390
Size: 10 305 195 777 267 441 436 1438
Cuts at: 5390 1521
Size: 2778
Fragments arranged by size:
2778 1438 777 441 436 305 267 195
10
ApaI G_GGCC'C
Cuts at: 1446 1599 2544 1446
Size: 153 945 5549
Fragments arranged by size:
5549 945 153
ApaLI G'TGCA_C
Cuts at: 448 727 5485 448
Size: 279 4758 1610
Fragments arranged by size:
4758 1610 279
ApoI r'AATT_y
Cuts at: 3651 6000 6256 3651
Size: 2349 256 4042
Fragments arranged by size:
4042 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6647
AvaI C'yCGr_G
Cuts at: 572 761 1769 1788 2630 3667 572
Size: 189 1008 19 842 1037 3552
Fragments arranged by size:
3552 1037 1008 842 189 19
BamHI G'GATC_C
Cuts at: 3672 3672
Size: 6647
BanI G'GyrC_C
Cuts at: 197 917 1030 2308 2583 3447 3663 4077 4325
Size: 720 113 1278 275 864 216 414 248
Cuts at: 4325 4360 197
Size: 35 2484
Fragments arranged by size:
2484 1278 864 720 414 275 248 216
113 35
BanII G_rGCy'C
Cuts at: 1446 1599 1997 2006 2544 2631 3347 3661 4691
Size: 153 398 9 538 87 716 314 1030
Cuts at: 4691 1446
Size: 3402
Fragments arranged by size:
3402 1030 716 538 398 314 153 87
9
BbeI G_GCGC'C
Cuts at: 201 3451 4081 4329 201
Size: 3250 630 248 2519
Fragments arranged by size:
3250 2519 630 248
BbsI GAAGACnn'nnnn_
Cuts at: 1480 2076 2118 3327 1480
Size: 596 42 1209 4800
Fragments arranged by size:
4800 1209 596 42
BbvCI CC'TCA_GC
Cuts at: 1163 1163
Size: 6647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3139 3173 90
Size: 34 3015 34 3564
Fragments arranged by size:
3564 3015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4545 5601 6647 4545
Size: 1056 1046 4545
Fragments arranged by size:
4545 1056 1046
BclI T'GATC_A
Cuts at: 4167 4167
Size: 6647
BglI GCCn_nnn'nGGC
Cuts at: 2458 2548 3499 2458
Size: 90 951 5606
Fragments arranged by size:
5606 951 90
BglII A'GATC_T
Cuts at: 851 4162 6199 851
Size: 3311 2037 1299
Fragments arranged by size:
3311 2037 1299
BlpI GC'TnA_GC
Cuts at: 3759 3759
Size: 6647
Bme1580I G_kGCm'C
Cuts at: 452 731 1446 1599 1706 1975 2469 2544 2588
Size: 279 715 153 107 269 494 75 44
Cuts at: 2588 4272 4365 5489 452
Size: 1684 93 1124 1610
Fragments arranged by size:
1684 1610 1124 715 494 279 269 153
107 93 75 44
BmgBI CAC'GTC
Cuts at: 632 2235 2432 2688 3429 632
Size: 1603 197 256 741 3850
Fragments arranged by size:
3850 1603 741 256 197
BmrI ACTGGGnnnn_n'
Cuts at: 1536 1935 2628 2856 3859 3940 4275 6419 1536
Size: 399 693 228 1003 81 335 2144 1764
Fragments arranged by size:
2144 1764 1003 693 399 335 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1101 3067 5014 1101
Size: 1966 1947 2734
Fragments arranged by size:
2734 1966 1947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 766 2495 3062 3097 3283 3652 223
Size: 543 1729 567 35 186 369 3218
Fragments arranged by size:
3218 1729 567 543 369 186 35
Bpu10I CC'TnA_GC
Cuts at: 1163 2906 1163
Size: 1743 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4702 5416 5714 3823
Size: 879 714 298 4756
Fragments arranged by size:
4756 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6351 6351
Size: 6647
BsaAI yAC'GTr
Cuts at: 1158 4000 1158
Size: 2842 3805
BsaBI GATnn'nnATC
Cuts at: 6 253 3472 4161 6198 6
Size: 247 3219 689 2037 455
Fragments arranged by size:
3219 2037 689 455 247
BsaHI Gr'CG_yC
Cuts at: 198 1823 1946 2830 3115 3448 4078 4326 5028
Size: 1625 123 884 285 333 630 248 702
Cuts at: 5028 6524 198
Size: 1496 321
Fragments arranged by size:
1625 1496 884 702 630 333 321 285
248 123
BsaWI w'CCGG_w
Cuts at: 515 1298 3836 4357 5035 5446 5593 5867 515
Size: 783 2538 521 678 411 147 274 1295
Fragments arranged by size:
2538 1295 783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 548 578 640 670 1360 1390 2189 2219 2468
Size: 30 62 30 690 30 799 30 249
Cuts at: 2468 2498 2584 2614 548
Size: 30 86 30 4581
Fragments arranged by size:
4581 799 690 249 86 62 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 754 763 1132 1946 2826 3121 3259 754
Size: 9 369 814 880 295 138 4142
Fragments arranged by size:
4142 880 814 369 295 138 9
BseYI C'CCAG_C
Cuts at: 282 1672 2169 2245 2530 2808 4072 4830 5495
Size: 1390 497 76 285 278 1264 758 665
Cuts at: 5495 5903 282
Size: 408 1026
Fragments arranged by size:
1390 1264 1026 758 665 497 408 285
278 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 382 406 865 868 1568 2346 2395 2640
Size: 124 24 459 3 700 778 49 245
Cuts at: 2640 2713 2893 3265 258
Size: 73 180 372 3640
Fragments arranged by size:
3640 778 700 459 372 245 180 124
73 49 24 3
BsiEI CG_ry'CG
Cuts at: 104 369 1284 1424 4189 4235 5465 104
Size: 265 915 140 2765 46 1230 1286
Fragments arranged by size:
2765 1286 1230 915 265 140 46
BsiHKAI G_wGCw'C
Cuts at: 452 731 1106 1505 1997 2006 3131 3347 3527
Size: 279 375 399 492 9 1125 216 180
Cuts at: 3527 3661 4439 4629 5489 452
Size: 134 778 190 860 1610
Fragments arranged by size:
1610 1125 860 778 492 399 375 279
216 190 180 134 9
Bsp1286I G_dGCh'C
Cuts at: 452 731 1106 1446 1505 1599 1706 1975 1997
Size: 279 375 340 59 94 107 269 22
Cuts at: 1997 2006 2469 2544 2588 2631 3131 3347 3527
Size: 9 463 75 44 43 500 216 180
Cuts at: 3527 3661 4272 4365 4439 4629 4691 5489 452
Size: 134 611 93 74 190 62 798 1610
Fragments arranged by size:
1610 798 611 500 463 375 340 279
269 216 190 180 134 107 94 93
75 74 62 59 44 43 22 9
BspEI T'CCGG_A
Cuts at: 1298 1298
Size: 6647
BspHI T'CATG_A
Cuts at: 5079 5079
Size: 6647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 1086 2735 3026 3321 3697 3911 4213 4594
Size: 682 1649 291 295 376 214 302 381
Cuts at: 4594 404
Size: 2457
Fragments arranged by size:
2457 1649 682 381 376 302 295 291
214
BsrBI CCG'CTC
Cuts at: 50 481 1304 2933 3267 4939 4993 6477 6642
Size: 431 823 1629 334 1672 54 1484 165
Cuts at: 6642 50
Size: 55
Fragments arranged by size:
1672 1629 1484 823 431 334 165 55
54
BsrDI GCAATG_nn'
Cuts at: 584 1394 4559 584
Size: 810 3165 2672
Fragments arranged by size:
3165 2672 810
BsrFI r'CCGG_y
Cuts at: 335 1279 3095 3319 4645 4826 335
Size: 944 1816 224 1326 181 2156
Fragments arranged by size:
2156 1816 1326 944 224 181
BssHII G'CGCG_C
Cuts at: 1258 1258
Size: 6647
BssSI C'ACGA_G
Cuts at: 649 1847 4918 5626 649
Size: 1198 3071 708 1670
Fragments arranged by size:
3071 1670 1198 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1500 2191 4024 1500
Size: 691 1833 4123
Fragments arranged by size:
4123 1833 691
BstBI TT'CG_AA
Cuts at: 5008 5008
Size: 6647
BstEII G'GTnAC_C
Cuts at: 1239 3552 1239
Size: 2313 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 2052 2847 3540 2052
Size: 795 693 5159
Fragments arranged by size:
5159 795 693
BstYI r'GATC_y
Cuts at: 1 851 1151 2702 3063 3672 4139 4162 4497
Size: 850 300 1551 361 609 467 23 335
Cuts at: 4497 4743 5049 5061 5147 6199 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1551 1052 850 609 467 449 361 335
306 300 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 139 1757 139
Size: 1618 5029
BtgI C'CryG_G
Cuts at: 129 369 832 1034 1401 1418 1679 2354 2588
Size: 240 463 202 367 17 261 675 234
Cuts at: 2588 2936 2973 3563 129
Size: 348 37 590 3213
Fragments arranged by size:
3213 675 590 463 367 348 261 240
234 202 37 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 564 1467 4597 4778 4941 564
Size: 903 3130 181 163 2270
Fragments arranged by size:
3130 2270 903 181 163
BtsI GCAGTG_nn'
Cuts at: 452 1706 1834 2263 2407 3176 3890 452
Size: 1254 128 429 144 769 714 3209
Fragments arranged by size:
3209 1254 769 714 429 144 128
DraI TTT'AAA
Cuts at: 3646 3646
Size: 6647
DrdI GACnn_nn'nnGTC
Cuts at: 1690 2080 4353 5697 1690
Size: 390 2273 1344 2640
Fragments arranged by size:
2640 2273 1344 390
EaeI y'GGCC_r
Cuts at: 233 366 422 782 1281 1421 1620 1841 2102
Size: 133 56 360 499 140 199 221 261
Cuts at: 2102 2237 2285 2336 2660 3296 3321 3500 4232
Size: 135 48 51 324 636 25 179 732
Cuts at: 4232 4406 4797 4824 6169 233
Size: 174 391 27 1345 711
Fragments arranged by size:
1345 732 711 636 499 391 360 324
261 221 199 179 174 140 135 133
56 51 48 27 25
EagI C'GGCC_G
Cuts at: 366 1281 1421 4232 366
Size: 915 140 2811 2781
Fragments arranged by size:
2811 2781 915 140
EarI CTCTTCn'nnn_
Cuts at: 1915 2285 2909 3223 4670 4880 1915
Size: 370 624 314 1447 210 3682
Fragments arranged by size:
3682 1447 624 370 314 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 1312 1329 2926 4855 5597 5743 6457 145
Size: 1167 17 1597 1929 742 146 714 335
Fragments arranged by size:
1929 1597 1167 742 714 335 146 17
EcoICRI GAG'CTC
Cuts at: 1995 2004 3345 3659 1995
Size: 9 1341 314 4983
Fragments arranged by size:
4983 1341 314 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 223 766 1800 1884 1896 2368 2495 3031
Size: 90 543 1034 84 12 472 127 536
Cuts at: 3031 3062 3097 3283 3652 4472 4904 5257 5919
Size: 31 35 186 369 820 432 353 662
Cuts at: 5919 133
Size: 861
Fragments arranged by size:
1034 861 820 662 543 536 472 432
369 353 186 127 90 84 35 31
12
EcoNI CCTnn'n_nnAGG
Cuts at: 640 1195 640
Size: 555 6092
EcoO109I rG'GnC_Cy
Cuts at: 135 1251 1442 1443 1753 2540 2541 2853 3015
Size: 1116 191 1 310 787 1 312 162
Cuts at: 3015 3229 3417 3786 135
Size: 214 188 369 2996
Fragments arranged by size:
2996 1116 787 369 312 310 214 191
188 162 1 1
EcoRI G'AATT_C
Cuts at: 3651 3651
Size: 6647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1086 4666 1086
Size: 3580 3067
FseI GG_CCGG'CC
Cuts at: 3323 3323
Size: 6647
FspI TGC'GCA
Cuts at: 1855 4428 1855
Size: 2573 4074
FspAI rTGC'GCAy
Cuts at: 1855 1855
Size: 6647
HaeII r_GCGC'y
Cuts at: 201 805 3451 4081 4329 5559 6330 201
Size: 604 2646 630 248 1230 771 518
Fragments arranged by size:
2646 1230 771 630 604 518 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1343 1375 2494 2526 3115 3147 3221 3253 4489
Size: 32 1119 32 589 32 74 32 1236
Cuts at: 4489 4519 4521 4551 1343
Size: 30 2 30 3439
Fragments arranged by size:
3439 1236 1119 589 74 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1083 3686 1083
Size: 2603 4044
KasI G'GCGC_C
Cuts at: 197 3447 4077 4325 197
Size: 3250 630 248 2519
Fragments arranged by size:
3250 2519 630 248
KpnI G_GTAC'C
Cuts at: 3667 3667
Size: 6647
MluI A'CGCG_T
Cuts at: 6080 6080
Size: 6647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 683 1666 5406 5590 683
Size: 983 3740 184 1740
Fragments arranged by size:
3740 1740 983 184
MscI TGG'CCA
Cuts at: 235 424 1843 2104 3502 4408 235
Size: 189 1419 261 1398 906 2474
Fragments arranged by size:
2474 1419 1398 906 261 189
MslI CAynn'nnrTG
Cuts at: 259 418 721 844 1291 1432 1849 2581 2824
Size: 159 303 123 447 141 417 732 243
Cuts at: 2824 3478 3538 4636 4763 259
Size: 654 60 1098 127 2143
Fragments arranged by size:
2143 1098 732 654 447 417 303 243
159 141 127 123 60
MspA1I CmG'CkG
Cuts at: 178 371 912 1575 2500 3357 3758 4072 4432
Size: 193 541 663 925 857 401 314 360
Cuts at: 4432 5216 5461 178
Size: 784 245 1364
Fragments arranged by size:
1364 925 857 784 663 541 401 360
314 245 193
NaeI GCC'GGC
Cuts at: 3321 4828 3321
Size: 1507 5140
NarI GG'CG_CC
Cuts at: 198 3448 4078 4326 198
Size: 3250 630 248 2519
Fragments arranged by size:
3250 2519 630 248
NcoI C'CATG_G
Cuts at: 129 2588 2973 3563 129
Size: 2459 385 590 3213
Fragments arranged by size:
3213 2459 590 385
NgoMIV G'CCGG_C
Cuts at: 3319 4826 3319
Size: 1507 5140
NspI r_CATG'y
Cuts at: 2166 3700 4200 5803 2166
Size: 1534 500 1603 3010
Fragments arranged by size:
3010 1603 1534 500
PasI CC'CwG_GG
Cuts at: 2707 2707
Size: 6647
PciI A'CATG_T
Cuts at: 5799 5799
Size: 6647
PflMI CCAn_nnn'nTGG
Cuts at: 927 1629 2748 2808 2868 927
Size: 702 1119 60 60 4706
Fragments arranged by size:
4706 1119 702 60 60
PfoI T'CCnGG_A
Cuts at: 1997 2384 3629 1997
Size: 387 1245 5015
Fragments arranged by size:
5015 1245 387
PmeI GTTT'AAAC
Cuts at: 3646 3646
Size: 6647
PmlI CAC'GTG
Cuts at: 1158 1158
Size: 6647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 5093
Size: 32 6615
PpuMI rG'GwC_Cy
Cuts at: 1251 2853 3015 3229 1251
Size: 1602 162 214 4669
Fragments arranged by size:
4669 1602 214 162
PspOMI G'GGCC_C
Cuts at: 1442 1595 2540 1442
Size: 153 945 5549
Fragments arranged by size:
5549 945 153
PspXI vC'TCGA_Gb
Cuts at: 1769 1769
Size: 6647
PstI C_TGCA'G
Cuts at: 1097 1496 1701 1991 2270 2954 3071 3227 3694
Size: 399 205 290 279 684 117 156 467
Cuts at: 3694 4379 1097
Size: 685 3365
Fragments arranged by size:
3365 685 684 467 399 290 279 205
156 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6647
PvuII CAG'CTG
Cuts at: 1575 2500 3357 4072 4432 1575
Size: 925 857 715 360 3790
Fragments arranged by size:
3790 925 857 715 360
RsrII CG'GwC_CG
Cuts at: 4842 4842
Size: 6647
SacI G_AGCT'C
Cuts at: 1997 2006 3347 3661 1997
Size: 9 1341 314 4983
Fragments arranged by size:
4983 1341 314 9
SacII CC_GC'GG
Cuts at: 372 372
Size: 6647
SalI G'TCGA_C
Cuts at: 3684 3684
Size: 6647
SanDI GG'GwC_CC
Cuts at: 3229 3229
Size: 6647
SapI GCTCTTCn'nnn_
Cuts at: 1915 2285 2909 4670 4880 1915
Size: 370 624 1761 210 3682
Fragments arranged by size:
3682 1761 624 370 210
SbfI CC_TGCA'GG
Cuts at: 1097 2954 3227 3694 1097
Size: 1857 273 467 4050
Fragments arranged by size:
4050 1857 467 273
ScaI AGT'ACT
Cuts at: 2260 2272 2617 6164 2260
Size: 12 345 3547 2743
Fragments arranged by size:
3547 2743 345 12
SexAI A'CCwGG_T
Cuts at: 1136 1136
Size: 6647
SfcI C'TryA_G
Cuts at: 33 1093 1492 1697 1987 2266 2607 2950 3067
Size: 1060 399 205 290 279 341 343 117
Cuts at: 3067 3223 3690 4375 5343 5534 33
Size: 156 467 685 968 191 1146
Fragments arranged by size:
1146 1060 968 685 467 399 343 341
290 279 205 191 156 117
SfiI GGCCn_nnn'nGGCC
Cuts at: 2548 3499 2548
Size: 951 5696
SfoI GGC'GCC
Cuts at: 199 3449 4079 4327 199
Size: 3250 630 248 2519
Fragments arranged by size:
3250 2519 630 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6647
SmaI CCC'GGG
Cuts at: 574 763 1790 3669 574
Size: 189 1027 1879 3552
Fragments arranged by size:
3552 1879 1027 189
SmlI C'TyrA_G
Cuts at: 1769 3802 4717 5431 5693 1769
Size: 2033 915 714 262 2723
Fragments arranged by size:
2723 2033 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6647
SphI G_CATG'C
Cuts at: 3700 4200 3700
Size: 500 6147
SrfI GCCC'GGGC
Cuts at: 574 574
Size: 6647
StuI AGG'CCT
Cuts at: 391 496 2785 391
Size: 105 2289 4253
Fragments arranged by size:
4253 2289 105
StyI C'CwwG_G
Cuts at: 129 425 1347 2588 2973 3022 3413 3494 3503
Size: 296 922 1241 385 49 391 81 9
Cuts at: 3503 3563 3596 3781 4135 129
Size: 60 33 185 354 2641
Fragments arranged by size:
2641 1241 922 391 385 354 296 185
81 60 49 33 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1555 1873 2326 2437 3397 3576 4230 4363 4822
Size: 318 453 111 960 179 654 133 459
Cuts at: 4822 5031 1555
Size: 209 3171
Fragments arranged by size:
3171 960 654 459 453 318 209 179
133 111
TatI w'GTAC_w
Cuts at: 2258 2270 2615 2717 6162 2258
Size: 12 345 102 3445 2743
Fragments arranged by size:
3445 2743 345 102 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 548 580 640 672 1360 1392 1638 1670 2189
Size: 32 60 32 688 32 246 32 519
Cuts at: 2189 2221 2399 2431 2468 2500 6164 6196 548
Size: 32 178 32 37 32 3664 32 999
Fragments arranged by size:
3664 999 688 519 246 178 60 37
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1138 4444 1138
Size: 3306 3341
XbaI T'CTAG_A
Cuts at: 3678 3678
Size: 6647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2166 2805 4142 2166
Size: 639 1337 4671
Fragments arranged by size:
4671 1337 639
XhoI C'TCGA_G
Cuts at: 1769 1769
Size: 6647
XmaI C'CCGG_G
Cuts at: 572 761 1788 3667 572
Size: 189 1027 1879 3552
Fragments arranged by size:
3552 1879 1027 189
XmnI GAAnn'nnTTC
Cuts at: 2993 2993
Size: 6647
ZraI GAC'GTC
Cuts at: 1947 1947
Size: 6647
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BstZ17I ClaI CspCI
DraIII EcoRV HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PsrI SgrAI SnaBI
SspI SwaI