Restriction map for
pF1KB3702
(Circular) (Six-base) MAPSORT of: pF1KB3702.seq Check: 8842 from: 1 to: 6227
pF1KB3702 6227 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 310 310
Size: 6227
AccI GT'mk_AC
Cuts at: 1319 1672 2967 3265 1319
Size: 353 1295 298 4281
Fragments arranged by size:
4281 1295 353 298
Acc65I G'GTAC_C
Cuts at: 3243 3243
Size: 6227
AclI AA'CG_TT
Cuts at: 6067 6067
Size: 6227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4052 4484 4837 5499 4052
Size: 432 353 662 4780
Fragments arranged by size:
4780 662 432 353
AflII C'TTAA_G
Cuts at: 3049 3049
Size: 6227
AflIII A'CryG_T
Cuts at: 1322 3027 3579 5379 5660 1322
Size: 1705 552 1800 281 1889
Fragments arranged by size:
1889 1800 1705 552 281
AleI CACnn'nnGTG
Cuts at: 992 992
Size: 6227
AlwNI CAG_nnn'CTG
Cuts at: 583 790 2887 3532 4970 583
Size: 207 2097 645 1438 1840
Fragments arranged by size:
2097 1840 1438 645 207
ApaI G_GGCC'C
Cuts at: 2637 2637
Size: 6227
ApaLI G'TGCA_C
Cuts at: 5065 5065
Size: 6227
ApoI r'AATT_y
Cuts at: 495 822 1860 2397 2422 3231 5580 5836 495
Size: 327 1038 537 25 809 2349 256 886
Fragments arranged by size:
2349 1038 886 809 537 327 256 25
AseI AT'TA_AT
Cuts at: 20 2687 20
Size: 2667 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6227
AvaI C'yCGr_G
Cuts at: 3247 3247
Size: 6227
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1384 1417 1384
Size: 33 6194
BamHI G'GATC_C
Cuts at: 3252 3252
Size: 6227
BanI G'GyrC_C
Cuts at: 138 1257 3059 3243 3657 3905 3940 138
Size: 1119 1802 184 414 248 35 2425
Fragments arranged by size:
2425 1802 1119 414 248 184 35
BanII G_rGCy'C
Cuts at: 1654 2414 2637 3241 4271 1654
Size: 760 223 604 1030 3610
Fragments arranged by size:
3610 1030 760 604 223
BbeI G_GCGC'C
Cuts at: 3063 3661 3909 3063
Size: 598 248 5381
Fragments arranged by size:
5381 598 248
BbsI GAAGACnn'nnnn_
Cuts at: 646 1556 1772 2096 646
Size: 910 216 324 4777
Fragments arranged by size:
4777 910 324 216
BbvCI CC'TCA_GC
Cuts at: 1598 1598
Size: 6227
BciVI GTATCCnnnnn_n'
Cuts at: 613 4125 5181 6227 613
Size: 3512 1056 1046 613
Fragments arranged by size:
3512 1056 1046 613
BclI T'GATC_A
Cuts at: 3747 3747
Size: 6227
BglII A'GATC_T
Cuts at: 2330 3742 5779 2330
Size: 1412 2037 2778
Fragments arranged by size:
2778 2037 1412
BlpI GC'TnA_GC
Cuts at: 3339 3339
Size: 6227
Bme1580I G_kGCm'C
Cuts at: 143 2637 3852 3945 5069 143
Size: 2494 1215 93 1124 1301
Fragments arranged by size:
2494 1301 1215 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1008 3439 3520 3855 5999 1008
Size: 2431 81 335 2144 1236
Fragments arranged by size:
2431 2144 1236 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4594 4594
Size: 6227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 2486
Size: 6227
Bpu10I CC'TnA_GC
Cuts at: 1598 1598
Size: 6227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1308 1519 2122 3403 4282 4996 5294 1308
Size: 211 603 1281 879 714 298 2241
Fragments arranged by size:
2241 1281 879 714 603 298 211
BsaI GGTCTCn'nnnn_
Cuts at: 368 632 1476 2361 5931 368
Size: 264 844 885 3570 664
Fragments arranged by size:
3570 885 844 664 264
BsaAI yAC'GTr
Cuts at: 3580 3580
Size: 6227
BsaBI GATnn'nnATC
Cuts at: 6 224 2156 3741 5778 6
Size: 218 1932 1585 2037 455
Fragments arranged by size:
2037 1932 1585 455 218
BsaHI Gr'CG_yC
Cuts at: 3060 3658 3906 4608 6104 3060
Size: 598 248 702 1496 3183
Fragments arranged by size:
3183 1496 702 598 248
BsaWI w'CCGG_w
Cuts at: 3416 3937 4615 5026 5173 5447 3416
Size: 521 678 411 147 274 4196
Fragments arranged by size:
4196 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 402 405 432 435 402
Size: 3 27 3 6194
Fragments arranged by size:
6194 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 410 1085 1743 410
Size: 675 658 4894
Fragments arranged by size:
4894 675 658
BseYI C'CCAG_C
Cuts at: 1903 3652 4410 5075 5483 1903
Size: 1749 758 665 408 2647
Fragments arranged by size:
2647 1749 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 1571
Size: 6227
BsiEI CG_ry'CG
Cuts at: 104 3769 3815 5045 104
Size: 3665 46 1230 1286
Fragments arranged by size:
3665 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2171 2913 3241 4019 4209 5069 2171
Size: 742 328 778 190 860 3329
Fragments arranged by size:
3329 860 778 742 328 190
BsmI GAATG_Cn'
Cuts at: 1815 1815
Size: 6227
BsmBI CGTCTCn'nnnn_
Cuts at: 2715 2715
Size: 6227
Bsp1286I G_dGCh'C
Cuts at: 143 1654 2171 2414 2637 2913 3241 3852 3945
Size: 1511 517 243 223 276 328 611 93
Cuts at: 3945 4019 4209 4271 5069 143
Size: 74 190 62 798 1301
Fragments arranged by size:
1511 1301 798 611 517 328 276 243
223 190 93 74 62
BspHI T'CATG_A
Cuts at: 264 4659 264
Size: 4395 1832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 1095 1446 2527 3277 3491 3793 4174 310
Size: 785 351 1081 750 214 302 381 2363
Fragments arranged by size:
2363 1081 785 750 381 351 302 214
BsrBI CCG'CTC
Cuts at: 50 4519 4573 6057 6222 50
Size: 4469 54 1484 165 55
Fragments arranged by size:
4469 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 563 875 4139 563
Size: 312 3264 2651
Fragments arranged by size:
3264 2651 312
BsrFI r'CCGG_y
Cuts at: 4225 4406 4225
Size: 181 6046
BsrGI T'GTAC_A
Cuts at: 1366 1366
Size: 6227
BssSI C'ACGA_G
Cuts at: 1067 1462 4498 5206 1067
Size: 395 3036 708 2088
Fragments arranged by size:
3036 2088 708 395
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 3604 1594
Size: 2010 4217
BstBI TT'CG_AA
Cuts at: 4588 4588
Size: 6227
BstXI CCAn_nnnn'nTGG
Cuts at: 165 1502 165
Size: 1337 4890
BstYI r'GATC_y
Cuts at: 1 219 375 961 2330 2883 2931 3252 3719
Size: 218 156 586 1369 553 48 321 467
Cuts at: 3719 3742 4077 4323 4629 4641 4727 5779 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1369 1052 586 553 467 449 335 321
306 246 218 156 86 48 23 12
BstZ17I GTA'TAC
Cuts at: 2968 2968
Size: 6227
BtgI C'CryG_G
Cuts at: 151 3188 151
Size: 3037 3190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4177 4358 4521 4177
Size: 181 163 5883
Fragments arranged by size:
5883 181 163
BtsI GCAGTG_nn'
Cuts at: 173 908 1529 3149 3176 3470 173
Size: 735 621 1620 27 294 2930
Fragments arranged by size:
2930 1620 735 621 294 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2022 2057 2022
Size: 35 6192
DraI TTT'AAA
Cuts at: 216 1109 3226 216
Size: 893 2117 3217
Fragments arranged by size:
3217 2117 893
DraIII CAC_nnn'GTG
Cuts at: 1594 2863 1594
Size: 1269 4958
DrdI GACnn_nn'nnGTC
Cuts at: 3103 3933 5277 3103
Size: 830 1344 4053
Fragments arranged by size:
4053 1344 830
EaeI y'GGCC_r
Cuts at: 735 2292 2604 2739 2785 2799 3812 3986 4377
Size: 1557 312 135 46 14 1013 174 391
Cuts at: 4377 4404 5749 735
Size: 27 1345 1213
Fragments arranged by size:
1557 1345 1213 1013 391 312 174 135
46 27 14
EagI C'GGCC_G
Cuts at: 3812 3812
Size: 6227
EarI CTCTTCn'nnn_
Cuts at: 1381 2901 4250 4460 1381
Size: 1520 1349 210 3148
Fragments arranged by size:
3148 1520 1349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4435 5177 5323 6037 4435
Size: 742 146 714 4625
Fragments arranged by size:
4625 742 714 146
EcoICRI GAG'CTC
Cuts at: 3239 3239
Size: 6227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 4052 4484 4837 5499 2486
Size: 1566 432 353 662 3214
Fragments arranged by size:
3214 1566 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 2633 3366 2633
Size: 733 5494
EcoRI G'AATT_C
Cuts at: 3231 3231
Size: 6227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 4246 2869
Size: 1377 4850
FspI TGC'GCA
Cuts at: 4008 4008
Size: 6227
HaeII r_GCGC'y
Cuts at: 3063 3661 3909 5139 5910 3063
Size: 598 248 1230 771 3380
Fragments arranged by size:
3380 1230 771 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1285 1317 2322 2354 2983 3015 4069 4099 4101
Size: 32 1005 32 629 32 1054 30 2
Cuts at: 4101 4131 1285
Size: 30 3381
Fragments arranged by size:
3381 1054 1005 629 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 483 632 2556 2984 3108 3266 483
Size: 149 1924 428 124 158 3444
Fragments arranged by size:
3444 1924 428 158 149 124
HpaI GTT'AAC
Cuts at: 2556 2556
Size: 6227
KasI G'GCGC_C
Cuts at: 3059 3657 3905 3059
Size: 598 248 5381
Fragments arranged by size:
5381 598 248
KpnI G_GTAC'C
Cuts at: 3247 3247
Size: 6227
MfeI C'AATT_G
Cuts at: 2051 2051
Size: 6227
MluI A'CGCG_T
Cuts at: 5660 5660
Size: 6227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 704 873 1407 4986 5170 704
Size: 169 534 3579 184 1761
Fragments arranged by size:
3579 1761 534 184 169
MscI TGG'CCA
Cuts at: 2294 2606 2741 3988 2294
Size: 312 135 1247 4533
Fragments arranged by size:
4533 1247 312 135
MslI CAynn'nnrTG
Cuts at: 269 992 4216 4343 269
Size: 723 3224 127 2153
Fragments arranged by size:
3224 2153 723 127
MspA1I CmG'CkG
Cuts at: 126 2079 3338 3652 4012 4796 5041 126
Size: 1953 1259 314 360 784 245 1312
Fragments arranged by size:
1953 1312 1259 784 360 314 245
NaeI GCC'GGC
Cuts at: 4408 4408
Size: 6227
NarI GG'CG_CC
Cuts at: 3060 3658 3906 3060
Size: 598 248 5381
Fragments arranged by size:
5381 598 248
NcoI C'CATG_G
Cuts at: 151 3188 151
Size: 3037 3190
NgoMIV G'CCGG_C
Cuts at: 4406 4406
Size: 6227
NspI r_CATG'y
Cuts at: 1326 2303 3280 3780 5383 1326
Size: 977 977 500 1603 2170
Fragments arranged by size:
2170 1603 977 977 500
PasI CC'CwG_GG
Cuts at: 1204 1381 1204
Size: 177 6050
PciI A'CATG_T
Cuts at: 1322 5379 1322
Size: 4057 2170
PflMI CCAn_nnn'nTGG
Cuts at: 1726 1978 1726
Size: 252 5975
PfoI T'CCnGG_A
Cuts at: 2951 2951
Size: 6227
PmeI GTTT'AAAC
Cuts at: 3226 3226
Size: 6227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 4673
Size: 32 6195
PsiI TTA'TAA
Cuts at: 1082 1082
Size: 6227
PspOMI G'GGCC_C
Cuts at: 2633 2633
Size: 6227
PstI C_TGCA'G
Cuts at: 125 915 3274 3959 125
Size: 790 2359 685 2393
Fragments arranged by size:
2393 2359 790 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6227
PvuII CAG'CTG
Cuts at: 2079 3652 4012 2079
Size: 1573 360 4294
Fragments arranged by size:
4294 1573 360
RsrII CG'GwC_CG
Cuts at: 4422 4422
Size: 6227
SacI G_AGCT'C
Cuts at: 3241 3241
Size: 6227
SalI G'TCGA_C
Cuts at: 3264 3264
Size: 6227
SapI GCTCTTCn'nnn_
Cuts at: 2901 4250 4460 2901
Size: 1349 210 4668
Fragments arranged by size:
4668 1349 210
SbfI CC_TGCA'GG
Cuts at: 3274 3274
Size: 6227
ScaI AGT'ACT
Cuts at: 949 1977 2211 2597 5744 949
Size: 1028 234 386 3147 1432
Fragments arranged by size:
3147 1432 1028 386 234
SfcI C'TryA_G
Cuts at: 33 121 911 1841 3270 3955 4923 5114 33
Size: 88 790 930 1429 685 968 191 1146
Fragments arranged by size:
1429 1146 968 930 790 685 191 88
SfoI GGC'GCC
Cuts at: 3061 3659 3907 3061
Size: 598 248 5381
Fragments arranged by size:
5381 598 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6227
SmaI CCC'GGG
Cuts at: 3249 3249
Size: 6227
SmlI C'TyrA_G
Cuts at: 1287 1534 2137 3049 3382 4297 5011 5273 1287
Size: 247 603 912 333 915 714 262 2241
Fragments arranged by size:
2241 915 912 714 603 333 262 247
SpeI A'CTAG_T
Cuts at: 67 2212 2374 67
Size: 2145 162 3920
Fragments arranged by size:
3920 2145 162
SphI G_CATG'C
Cuts at: 3280 3780 3280
Size: 500 5727
SspI AAT'ATT
Cuts at: 281 931 1803 2685 281
Size: 650 872 882 3823
Fragments arranged by size:
3823 882 872 650
StuI AGG'CCT
Cuts at: 2919 2919
Size: 6227
StyI C'CwwG_G
Cuts at: 151 237 770 3188 3361 3715 151
Size: 86 533 2418 173 354 2663
Fragments arranged by size:
2663 2418 533 354 173 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3810 3943 4402 4611 3810
Size: 133 459 209 5426
Fragments arranged by size:
5426 459 209 133
TatI w'GTAC_w
Cuts at: 947 1366 1975 2209 2595 5742 947
Size: 419 609 234 386 3147 1432
Fragments arranged by size:
3147 1432 609 419 386 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5744 5776 5744
Size: 32 6195
Tth111I GACn'n_nGTC
Cuts at: 4024 4024
Size: 6227
XbaI T'CTAG_A
Cuts at: 705 3258 705
Size: 2553 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2529 2584 2949 3090 3722 2529
Size: 55 365 141 632 5034
Fragments arranged by size:
5034 632 365 141 55
XmaI C'CCGG_G
Cuts at: 3247 3247
Size: 6227
XmnI GAAnn'nnTTC
Cuts at: 2753 2808 2753
Size: 55 6172
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AlfI AloI AscI AvrII
BcgI BfrBI BglI BmgBI BmtI BsiWI BspEI BssHII
BstEII Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI