Restriction map for
pF1KB3707
(Circular) (Six-base) MAPSORT of: pF1KB3707.seq Check: 1849 from: 1 to: 6110
pF1KB3707 6110 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1233 1953 1233
Size: 720 5390
AccI GT'mk_AC
Cuts at: 1433 3148 1433
Size: 1715 4395
Acc65I G'GTAC_C
Cuts at: 1870 3126 1870
Size: 1256 4854
AclI AA'CG_TT
Cuts at: 2674 5950 2674
Size: 3276 2834
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1318 1513 1643 2260 2972 3935 4367 4720
Size: 707 195 130 617 712 963 432 353
Cuts at: 4720 5382 611
Size: 662 1339
Fragments arranged by size:
1339 963 712 707 662 617 432 353
195 130
AflIII A'CryG_T
Cuts at: 3462 5262 5543 3462
Size: 1800 281 4029
Fragments arranged by size:
4029 1800 281
AgeI A'CCGG_T
Cuts at: 312 312
Size: 6110
AhdI GACnn_n'nnGTC
Cuts at: 1710 1710
Size: 6110
AleI CACnn'nnGTG
Cuts at: 832 2479 2659 832
Size: 1647 180 4283
Fragments arranged by size:
4283 1647 180
AlwNI CAG_nnn'CTG
Cuts at: 1653 2128 2820 2977 3049 3415 4853 1653
Size: 475 692 157 72 366 1438 2910
Fragments arranged by size:
2910 1438 692 475 366 157 72
ApaI G_GGCC'C
Cuts at: 1532 2625 1532
Size: 1093 5017
ApaLI G'TGCA_C
Cuts at: 508 4948 508
Size: 4440 1670
ApoI r'AATT_y
Cuts at: 1147 3114 5463 5719 1147
Size: 1967 2349 256 1538
Fragments arranged by size:
2349 1967 1538 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6110
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6110
AvaI C'yCGr_G
Cuts at: 1268 2377 2879 3130 1268
Size: 1109 502 251 4248
Fragments arranged by size:
4248 1109 502 251
BamHI G'GATC_C
Cuts at: 2995 3135 2995
Size: 140 5970
BanI G'GyrC_C
Cuts at: 1182 1870 3126 3540 3788 3823 1182
Size: 688 1256 414 248 35 3469
Fragments arranged by size:
3469 1256 688 414 248 35
BanII G_rGCy'C
Cuts at: 811 938 1532 1930 1973 2625 2966 3124 4154
Size: 127 594 398 43 652 341 158 1030
Cuts at: 4154 811
Size: 2767
Fragments arranged by size:
2767 1030 652 594 398 341 158 127
43
BbeI G_GCGC'C
Cuts at: 3544 3792 3544
Size: 248 5862
BbsI GAAGACnn'nnnn_
Cuts at: 633 744 859 633
Size: 111 115 5884
Fragments arranged by size:
5884 115 111
BbvCI CC'TCA_GC
Cuts at: 739 1985 2509 739
Size: 1246 524 4340
Fragments arranged by size:
4340 1246 524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 664 698 664
Size: 34 6076
BciVI GTATCCnnnnn_n'
Cuts at: 1709 4008 5064 6110 1709
Size: 2299 1056 1046 1709
Fragments arranged by size:
2299 1709 1056 1046
BclI T'GATC_A
Cuts at: 626 3630 626
Size: 3004 3106
BglI GCCn_nnn'nGGC
Cuts at: 141 163 1360 2568 2731 141
Size: 22 1197 1208 163 3520
Fragments arranged by size:
3520 1208 1197 163 22
BglII A'GATC_T
Cuts at: 1626 2417 2648 3625 5662 1626
Size: 791 231 977 2037 2074
Fragments arranged by size:
2074 2037 977 791 231
BlpI GC'TnA_GC
Cuts at: 1384 2431 2504 2735 3222 1384
Size: 1047 73 231 487 4272
Fragments arranged by size:
4272 1047 487 231 73
Bme1580I G_kGCm'C
Cuts at: 272 512 1532 2625 3735 3828 4952 272
Size: 240 1020 1093 1110 93 1124 1430
Fragments arranged by size:
1430 1124 1110 1093 1020 240 93
BmrI ACTGGGnnnn_n'
Cuts at: 1987 3322 3403 3738 5882 1987
Size: 1335 81 335 2144 2215
Fragments arranged by size:
2215 2144 1335 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2022 2436 4477 2022
Size: 414 2041 3655
Fragments arranged by size:
3655 2041 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 946 1192 2126 2320 2473 2921 3059 284
Size: 662 246 934 194 153 448 138 3335
Fragments arranged by size:
3335 934 662 448 246 194 153 138
Bpu10I CC'TnA_GC
Cuts at: 739 810 1985 2509 739
Size: 71 1175 524 4340
Fragments arranged by size:
4340 1175 524 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1957 2599 3286 4165 4879 5177 1957
Size: 642 687 879 714 298 2890
Fragments arranged by size:
2890 879 714 687 642 298
BsaI GGTCTCn'nnnn_
Cuts at: 2288 2347 2689 5814 2288
Size: 59 342 3125 2584
Fragments arranged by size:
3125 2584 342 59
BsaAI yAC'GTr
Cuts at: 3463 3463
Size: 6110
BsaBI GATnn'nnATC
Cuts at: 6 3624 5661 6
Size: 3618 2037 455
Fragments arranged by size:
3618 2037 455
BsaHI Gr'CG_yC
Cuts at: 2135 3541 3789 4491 5987 2135
Size: 1406 248 702 1496 2258
Fragments arranged by size:
2258 1496 1406 702 248
BsaWI w'CCGG_w
Cuts at: 312 3299 3820 4498 4909 5056 5330 312
Size: 2987 521 678 411 147 274 1092
Fragments arranged by size:
2987 1092 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 153 1168 1330 1483 1486 1936 2212 2416 2428
Size: 1015 162 153 3 450 276 204 12
Cuts at: 2428 2560 2581 153
Size: 132 21 3682
Fragments arranged by size:
3682 1015 450 276 204 162 153 132
21 12 3
BseYI C'CCAG_C
Cuts at: 865 958 1320 3535 4293 4958 5366 865
Size: 93 362 2215 758 665 408 1609
Fragments arranged by size:
2215 1609 758 665 408 362 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 1184 2287 2833 380
Size: 804 1103 546 3657
Fragments arranged by size:
3657 1103 804 546
BsiEI CG_ry'CG
Cuts at: 104 3652 3698 4928 104
Size: 3548 46 1230 1286
Fragments arranged by size:
3548 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 173 476 512 938 1760 1973 3124 3902 4092
Size: 303 36 426 822 213 1151 778 190
Cuts at: 4092 4952 173
Size: 860 1331
Fragments arranged by size:
1331 1151 860 822 778 426 303 213
190 36
BsmI GAATG_Cn'
Cuts at: 2718 2718
Size: 6110
Bsp1286I G_dGCh'C
Cuts at: 173 272 476 512 811 938 1532 1760 1930
Size: 99 204 36 299 127 594 228 170
Cuts at: 1930 1973 2625 2966 3124 3735 3828 3902 4092
Size: 43 652 341 158 611 93 74 190
Cuts at: 4092 4154 4952 173
Size: 62 798 1331
Fragments arranged by size:
1331 798 652 611 594 341 299 228
204 190 170 158 127 99 93 74
62 43 36
BspHI T'CATG_A
Cuts at: 4542 4542
Size: 6110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1233 1953 2237 3160 3374 3676 4057 1233
Size: 720 284 923 214 302 381 3286
Fragments arranged by size:
3286 923 720 381 302 284 214
BsrBI CCG'CTC
Cuts at: 50 1179 4402 4456 5940 6105 50
Size: 1129 3223 54 1484 165 55
Fragments arranged by size:
3223 1484 1129 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4022 4022
Size: 6110
BsrFI r'CCGG_y
Cuts at: 312 4108 4289 312
Size: 3796 181 2133
Fragments arranged by size:
3796 2133 181
BsrGI T'GTAC_A
Cuts at: 1194 1194
Size: 6110
BssSI C'ACGA_G
Cuts at: 475 1139 1455 4381 5089 475
Size: 664 316 2926 708 1496
Fragments arranged by size:
2926 1496 708 664 316
BstAPI GCAn_nnn'nTGC
Cuts at: 507 824 2820 3487 507
Size: 317 1996 667 3130
Fragments arranged by size:
3130 1996 667 317
BstBI TT'CG_AA
Cuts at: 4471 4471
Size: 6110
BstXI CCAn_nnnn'nTGG
Cuts at: 246 1446 2334 2451 246
Size: 1200 888 117 3905
Fragments arranged by size:
3905 1200 888 117
BstYI r'GATC_y
Cuts at: 1 887 1626 2417 2648 2995 3135 3602 3625
Size: 886 739 791 231 347 140 467 23
Cuts at: 3625 3960 4206 4512 4524 4610 5662 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 886 791 739 467 449 347 335
306 246 231 140 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 545 2030 2048 2129 2861 545
Size: 1485 18 81 732 3794
Fragments arranged by size:
3794 1485 732 81 18
BtgI C'CryG_G
Cuts at: 107 272 1022 1304 1355 107
Size: 165 750 282 51 4862
Fragments arranged by size:
4862 750 282 165 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4060 4241 4404 4060
Size: 181 163 5766
Fragments arranged by size:
5766 181 163
BtsI GCAGTG_nn'
Cuts at: 215 237 629 731 776 1370 1433 3353 215
Size: 22 392 102 45 594 63 1920 2972
Fragments arranged by size:
2972 1920 594 392 102 63 45 22
DraI TTT'AAA
Cuts at: 1398 3109 1398
Size: 1711 4399
DraIII CAC_nnn'GTG
Cuts at: 196 196
Size: 6110
DrdI GACnn_nn'nnGTC
Cuts at: 3816 5160 3816
Size: 1344 4766
EaeI y'GGCC_r
Cuts at: 248 1025 2723 3695 3869 4260 4287 5632 248
Size: 777 1698 972 174 391 27 1345 726
Fragments arranged by size:
1698 1345 972 777 726 391 174 27
EagI C'GGCC_G
Cuts at: 3695 3695
Size: 6110
EarI CTCTTCn'nnn_
Cuts at: 1519 1808 1913 4133 4343 1519
Size: 289 105 2220 210 3286
Fragments arranged by size:
3286 2220 289 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 745 4318 5060 5206 5920 745
Size: 3573 742 146 714 935
Fragments arranged by size:
3573 935 742 714 146
EcoICRI GAG'CTC
Cuts at: 936 1971 3122 936
Size: 1035 1151 3924
Fragments arranged by size:
3924 1151 1035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 611 946 1192 1318 1513 1643 2126 2260
Size: 327 335 246 126 195 130 483 134
Cuts at: 2260 2320 2473 2921 2972 3059 3935 4367 4720
Size: 60 153 448 51 87 876 432 353
Cuts at: 4720 5382 284
Size: 662 1012
Fragments arranged by size:
1012 876 662 483 448 432 353 335
327 246 195 153 134 130 126 87
60 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2939 2939
Size: 6110
EcoO109I rG'GnC_Cy
Cuts at: 921 1203 1272 1528 1529 1618 1989 2125 2621
Size: 282 69 256 1 89 371 136 496
Cuts at: 2621 2876 3034 3249 921
Size: 255 158 215 3782
Fragments arranged by size:
3782 496 371 282 256 255 215 158
136 89 69 1
EcoRI G'AATT_C
Cuts at: 3114 3114
Size: 6110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1749 4129 1749
Size: 2380 3730
FspI TGC'GCA
Cuts at: 3891 3891
Size: 6110
HaeII r_GCGC'y
Cuts at: 663 2438 3544 3792 5022 5793 663
Size: 1775 1106 248 1230 771 980
Fragments arranged by size:
1775 1230 1106 980 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 366 398 793 825 1696 1728 2980 3012 3952
Size: 32 395 32 871 32 1252 32 940
Cuts at: 3952 3982 3984 4014 366
Size: 30 2 30 2462
Fragments arranged by size:
2462 1252 940 871 395 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3149 3149
Size: 6110
KasI G'GCGC_C
Cuts at: 3540 3788 3540
Size: 248 5862
KpnI G_GTAC'C
Cuts at: 1874 3130 1874
Size: 1256 4854
MluI A'CGCG_T
Cuts at: 5543 5543
Size: 6110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2448 4869 5053 2448
Size: 2421 184 3505
Fragments arranged by size:
3505 2421 184
MscI TGG'CCA
Cuts at: 250 1027 3871 250
Size: 777 2844 2489
Fragments arranged by size:
2844 2489 777
MslI CAynn'nnrTG
Cuts at: 244 832 1444 2479 2482 2659 4099 4226 244
Size: 588 612 1035 3 177 1440 127 2128
Fragments arranged by size:
2128 1440 1035 612 588 177 127 3
MspA1I CmG'CkG
Cuts at: 531 882 1378 3221 3535 3895 4679 4924 531
Size: 351 496 1843 314 360 784 245 1717
Fragments arranged by size:
1843 1717 784 496 360 351 314 245
NaeI GCC'GGC
Cuts at: 4291 4291
Size: 6110
NarI GG'CG_CC
Cuts at: 3541 3789 3541
Size: 248 5862
NcoI C'CATG_G
Cuts at: 107 272 1022 1355 107
Size: 165 750 333 4862
Fragments arranged by size:
4862 750 333 165
NgoMIV G'CCGG_C
Cuts at: 4289 4289
Size: 6110
NspI r_CATG'y
Cuts at: 2610 3163 3663 5266 2610
Size: 553 500 1603 3454
Fragments arranged by size:
3454 1603 553 500
PasI CC'CwG_GG
Cuts at: 915 915
Size: 6110
PciI A'CATG_T
Cuts at: 5262 5262
Size: 6110
PfoI T'CCnGG_A
Cuts at: 467 1910 3036 467
Size: 1443 1126 3541
Fragments arranged by size:
3541 1443 1126
PmeI GTTT'AAAC
Cuts at: 3109 3109
Size: 6110
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 4556
Size: 32 6078
PpuMI rG'GwC_Cy
Cuts at: 1272 2876 3034 1272
Size: 1604 158 4348
Fragments arranged by size:
4348 1604 158
PspOMI G'GGCC_C
Cuts at: 1528 2621 1528
Size: 1093 5017
PstI C_TGCA'G
Cuts at: 536 677 710 887 1935 3157 3842 536
Size: 141 33 177 1048 1222 685 2804
Fragments arranged by size:
2804 1222 1048 685 177 141 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6110
PvuII CAG'CTG
Cuts at: 531 882 1378 3535 3895 531
Size: 351 496 2157 360 2746
Fragments arranged by size:
2746 2157 496 360 351
RsrII CG'GwC_CG
Cuts at: 4305 4305
Size: 6110
SacI G_AGCT'C
Cuts at: 938 1973 3124 938
Size: 1035 1151 3924
Fragments arranged by size:
3924 1151 1035
SalI G'TCGA_C
Cuts at: 3147 3147
Size: 6110
SanDI GG'GwC_CC
Cuts at: 1272 2876 1272
Size: 1604 4506
SapI GCTCTTCn'nnn_
Cuts at: 4133 4343 4133
Size: 210 5900
SbfI CC_TGCA'GG
Cuts at: 3157 3157
Size: 6110
ScaI AGT'ACT
Cuts at: 5627 5627
Size: 6110
SfcI C'TryA_G
Cuts at: 33 532 673 706 883 1566 1931 3153 3838
Size: 499 141 33 177 683 365 1222 685
Cuts at: 3838 4806 4997 33
Size: 968 191 1146
Fragments arranged by size:
1222 1146 968 685 683 499 365 191
177 141 33
SfoI GGC'GCC
Cuts at: 3542 3790 3542
Size: 248 5862
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6110
SgrAI Cr'CCGG_yG
Cuts at: 312 312
Size: 6110
SmaI CCC'GGG
Cuts at: 2881 3132 2881
Size: 251 5859
SmlI C'TyrA_G
Cuts at: 1972 2614 3265 4180 4894 5156 1972
Size: 642 651 915 714 262 2926
Fragments arranged by size:
2926 915 714 651 642 262
SpeI A'CTAG_T
Cuts at: 67 3102 67
Size: 3035 3075
SphI G_CATG'C
Cuts at: 3163 3663 3163
Size: 500 5610
StuI AGG'CCT
Cuts at: 1103 1103
Size: 6110
StyI C'CwwG_G
Cuts at: 107 158 272 1022 1355 1715 1886 2523 3244
Size: 51 114 750 333 360 171 637 721
Cuts at: 3244 3598 107
Size: 354 2619
Fragments arranged by size:
2619 750 721 637 360 354 333 171
114 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1246 1669 2819 3693 3826 4285 4494 1246
Size: 423 1150 874 133 459 209 2862
Fragments arranged by size:
2862 1150 874 459 423 209 133
TatI w'GTAC_w
Cuts at: 1194 5625 1194
Size: 4431 1679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5627 5659 5627
Size: 32 6078
Tth111I GACn'n_nGTC
Cuts at: 3907 3907
Size: 6110
XbaI T'CTAG_A
Cuts at: 1764 3141 1764
Size: 1377 4733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3605 3605
Size: 6110
XmaI C'CCGG_G
Cuts at: 2879 3130 2879
Size: 251 5859
XmnI GAAnn'nnTTC
Cuts at: 1628 1628
Size: 6110
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BspEI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SnaBI SrfI SspI SwaI XhoI ZraI