Restriction map for
pF1KB3719
(Circular) (Six-base) MAPSORT of: pF1KB3719.seq Check: 9538 from: 1 to: 5711
pF1KB3719 5711 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 605 2075 605
Size: 1470 4241
AatII G_ACGT'C
Cuts at: 1646 1646
Size: 5711
AccI GT'mk_AC
Cuts at: 2415 2749 2415
Size: 334 5377
Acc65I G'GTAC_C
Cuts at: 1673 2727 1673
Size: 1054 4657
AclI AA'CG_TT
Cuts at: 477 5551 477
Size: 5074 637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 951 1252 1602 3536 3968 4715 4983 528
Size: 423 301 350 1934 432 747 268 1256
Fragments arranged by size:
1934 1256 747 432 423 350 301 268
AflIII A'CryG_T
Cuts at: 3063 4167 5144 3063
Size: 1104 977 3630
Fragments arranged by size:
3630 1104 977
AgeI A'CCGG_T
Cuts at: 4099 4099
Size: 5711
AleI CACnn'nnGTG
Cuts at: 1333 2110 1333
Size: 777 4934
AlwNI CAG_nnn'CTG
Cuts at: 804 974 1311 1722 2385 3016 4583 804
Size: 170 337 411 663 631 1567 1932
Fragments arranged by size:
1932 1567 663 631 411 337 170
ApaI G_GGCC'C
Cuts at: 149 2136 2162 2699 149
Size: 1987 26 537 3161
Fragments arranged by size:
3161 1987 537 26
ApaLI G'TGCA_C
Cuts at: 4481 4481
Size: 5711
ApoI r'AATT_y
Cuts at: 642 1297 2715 5064 5320 642
Size: 655 1418 2349 256 1033
Fragments arranged by size:
2349 1418 1033 655 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5711
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
AvaI C'yCGr_G
Cuts at: 141 189 433 1791 2075 2232 2275 2521 2596
Size: 48 244 1358 284 157 43 246 75
Cuts at: 2596 2661 2731 141
Size: 65 70 3121
Fragments arranged by size:
3121 1358 284 246 244 157 75 70
65 48 43
BamHI G'GATC_C
Cuts at: 2736 2736
Size: 5711
BanI G'GyrC_C
Cuts at: 196 1494 1597 1665 1673 1968 2173 2727 3141
Size: 1298 103 68 8 295 205 554 414
Cuts at: 3141 3389 3424 196
Size: 248 35 2483
Fragments arranged by size:
2483 1298 554 414 295 248 205 103
68 35 8
BanII G_rGCy'C
Cuts at: 149 703 1111 1792 2136 2162 2509 2699 2725
Size: 554 408 681 344 26 347 190 26
Cuts at: 2725 3755 149
Size: 1030 2105
Fragments arranged by size:
2105 1030 681 554 408 347 344 190
26 26
BbeI G_GCGC'C
Cuts at: 200 1498 1669 1972 3145 3393 200
Size: 1298 171 303 1173 248 2518
Fragments arranged by size:
2518 1298 1173 303 248 171
BciVI GTATCCnnnnn_n'
Cuts at: 3609 4370 5711 3609
Size: 761 1341 3609
Fragments arranged by size:
3609 1341 761
BclI T'GATC_A
Cuts at: 3231 3231
Size: 5711
BfrBI ATG'CAT
Cuts at: 695 695
Size: 5711
BglI GCCn_nnn'nGGC
Cuts at: 112 1503 1796 2005 2140 112
Size: 1391 293 209 135 3683
Fragments arranged by size:
3683 1391 293 209 135
BglII A'GATC_T
Cuts at: 3226 5263 3226
Size: 2037 3674
BlpI GC'TnA_GC
Cuts at: 2823 2823
Size: 5711
Bme1580I G_kGCm'C
Cuts at: 149 2136 2162 2699 3336 3429 4485 149
Size: 1987 26 537 637 93 1056 1375
Fragments arranged by size:
1987 1375 1056 637 537 93 26
BmgBI CAC'GTC
Cuts at: 1287 2337 1287
Size: 1050 4661
BmrI ACTGGGnnnn_n'
Cuts at: 1299 2693 2923 3004 3339 5483 1299
Size: 1394 230 81 335 2144 1527
Fragments arranged by size:
2144 1527 1394 335 230 81
BmtI G_CTAG'C
Cuts at: 1150 1150
Size: 5711
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1443 2007 4078 1443
Size: 564 2071 3076
Fragments arranged by size:
3076 2071 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1019 1161 1744 2484 2608 2626 763
Size: 256 142 583 740 124 18 3848
Fragments arranged by size:
3848 740 583 256 142 124 18
Bpu10I CC'TnA_GC
Cuts at: 702 702
Size: 5711
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2887 3766 4258 4556 2887
Size: 879 492 298 4042
Fragments arranged by size:
4042 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 365 730 5415 365
Size: 365 4685 661
Fragments arranged by size:
4685 661 365
BsaAI yAC'GTr
Cuts at: 1281 1605 3064 1281
Size: 324 1459 3928
Fragments arranged by size:
3928 1459 324
BsaBI GATnn'nnATC
Cuts at: 6 3225 5262 6
Size: 3219 2037 455
Fragments arranged by size:
3219 2037 455
BsaHI Gr'CG_yC
Cuts at: 197 416 1495 1643 1666 1969 2311 3142 3390
Size: 219 1079 148 23 303 342 831 248
Cuts at: 3390 4092 5588 197
Size: 702 1496 320
Fragments arranged by size:
1496 1079 831 702 342 320 303 248
219 148 23
BsaWI w'CCGG_w
Cuts at: 1047 2900 3421 4099 4373 4520 4931 1047
Size: 1853 521 678 274 147 411 1827
Fragments arranged by size:
1853 1827 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2030 2060 2030
Size: 30 5681
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 309 1711 1942 2051 2140 2470 2473 2563 2572
Size: 1402 231 109 89 330 3 90 9
Cuts at: 2572 2631 309
Size: 59 3389
Fragments arranged by size:
3389 1402 330 231 109 90 89 59
9 3
BseYI C'CCAG_C
Cuts at: 153 239 1150 2223 2239 2666 3136 3894 4471
Size: 86 911 1073 16 427 470 758 577
Cuts at: 4471 4967 153
Size: 496 897
Fragments arranged by size:
1073 911 897 758 577 496 470 427
86 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 331 461 970 2497 2723 240
Size: 91 130 509 1527 226 3228
Fragments arranged by size:
3228 1527 509 226 130 91
BsiEI CG_ry'CG
Cuts at: 104 3253 3299 4507 104
Size: 3149 46 1208 1308
Fragments arranged by size:
3149 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 668 703 851 1450 2117 2348 2509 2725 3503
Size: 35 148 599 667 231 161 216 778
Cuts at: 3503 3693 4485 668
Size: 190 792 1894
Fragments arranged by size:
1894 792 778 667 599 231 216 190
161 148 35
BsmBI CGTCTCn'nnnn_
Cuts at: 2318 2318
Size: 5711
Bsp1286I G_dGCh'C
Cuts at: 149 668 703 851 1111 1450 1792 2117 2136
Size: 519 35 148 260 339 342 325 19
Cuts at: 2136 2162 2348 2509 2699 2725 3336 3429 3503
Size: 26 186 161 190 26 611 93 74
Cuts at: 3503 3693 3755 4485 149
Size: 190 62 730 1375
Fragments arranged by size:
1375 730 611 519 342 339 325 260
190 190 186 161 148 93 74 62
35 26 26 19
BspEI T'CCGG_A
Cuts at: 1047 4931 1047
Size: 3884 1827
BspHI T'CATG_A
Cuts at: 1382 4887 1382
Size: 3505 2206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 283 341 605 873 1355 1790 1830 2075 2761
Size: 58 264 268 482 435 40 245 686
Cuts at: 2761 2975 3277 3658 283
Size: 214 302 381 2336
Fragments arranged by size:
2336 686 482 435 381 302 268 264
245 214 58 40
BsrBI CCG'CTC
Cuts at: 50 1680 4003 4057 5541 5706 50
Size: 1630 2323 54 1484 165 55
Fragments arranged by size:
2323 1630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3623 3623
Size: 5711
BsrFI r'CCGG_y
Cuts at: 1951 2577 3709 3890 4099 1951
Size: 626 1132 181 209 3563
Fragments arranged by size:
3563 1132 626 209 181
BssHII G'CGCG_C
Cuts at: 1461 1461
Size: 5711
BssSI C'ACGA_G
Cuts at: 3982 4340 3982
Size: 358 5353
BstAPI GCAn_nnn'nTGC
Cuts at: 2475 3088 2475
Size: 613 5098
BstBI TT'CG_AA
Cuts at: 1862 4072 1862
Size: 2210 3501
BstEII G'GTnAC_C
Cuts at: 582 681 582
Size: 99 5612
BstXI CCAn_nnnn'nTGG
Cuts at: 1101 1101
Size: 5711
BstYI r'GATC_y
Cuts at: 1 338 800 953 2736 3203 3226 3561 3807
Size: 337 462 153 1783 467 23 335 246
Cuts at: 3807 4819 4905 4917 5263 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1783 1012 467 462 449 346 337 335
246 153 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2614 2614
Size: 5711
BtgI C'CryG_G
Cuts at: 107 383 731 1452 1518 1529 1669 2144 2267
Size: 276 348 721 66 11 140 475 123
Cuts at: 2267 2306 2423 107
Size: 39 117 3395
Fragments arranged by size:
3395 721 475 348 276 140 123 117
66 39 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3661 3842 4005 3661
Size: 181 163 5367
Fragments arranged by size:
5367 181 163
BtsI GCAGTG_nn'
Cuts at: 403 1544 1933 2051 2954 403
Size: 1141 389 118 903 3160
Fragments arranged by size:
3160 1141 903 389 118
DraI TTT'AAA
Cuts at: 2710 2710
Size: 5711
DraIII CAC_nnn'GTG
Cuts at: 555 776 948 555
Size: 221 172 5318
Fragments arranged by size:
5318 221 172
DrdI GACnn_nn'nnGTC
Cuts at: 1642 1840 2020 3417 4275 1642
Size: 198 180 1397 858 3078
Fragments arranged by size:
3078 1397 858 198 180
EaeI y'GGCC_r
Cuts at: 2006 2141 3296 3470 3861 3888 5233 2006
Size: 135 1155 174 391 27 1345 2484
Fragments arranged by size:
2484 1345 1155 391 174 135 27
EagI C'GGCC_G
Cuts at: 3296 3296
Size: 5711
EarI CTCTTCn'nnn_
Cuts at: 875 1349 1910 2263 2377 2540 2549 3734 3944
Size: 474 561 353 114 163 9 1185 210
Cuts at: 3944 875
Size: 2642
Fragments arranged by size:
2642 1185 561 474 353 210 163 114
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2632 3919 4229 4375 5521 2632
Size: 1287 310 146 1146 2822
Fragments arranged by size:
2822 1287 1146 310 146
EcoICRI GAG'CTC
Cuts at: 701 2507 2723 701
Size: 1806 216 3689
Fragments arranged by size:
3689 1806 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 763 951 1019 1161 1252 1602 1744 2484
Size: 235 188 68 142 91 350 142 740
Cuts at: 2484 2608 2626 3536 3968 4715 4983 528
Size: 124 18 910 432 747 268 1256
Fragments arranged by size:
1256 910 747 740 432 350 268 235
188 142 142 124 91 68 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2329 2329
Size: 5711
EcoO109I rG'GnC_Cy
Cuts at: 145 146 330 621 717 1536 1719 2132 2133
Size: 1 184 291 96 819 183 413 1
Cuts at: 2133 2159 2565 2601 2695 2850 145
Size: 26 406 36 94 155 3006
Fragments arranged by size:
3006 819 413 406 291 184 183 155
96 94 36 26 1 1
EcoRI G'AATT_C
Cuts at: 2715 2715
Size: 5711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3730 3730
Size: 5711
FspI TGC'GCA
Cuts at: 3492 3492
Size: 5711
HaeII r_GCGC'y
Cuts at: 200 229 1480 1498 1669 1972 2092 2240 3145
Size: 29 1251 18 171 303 120 148 905
Cuts at: 3145 3393 4415 5394 200
Size: 248 1022 979 517
Fragments arranged by size:
1251 1022 979 905 517 303 248 171
148 120 29 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 487 519 887 919 1888 1920 3553
Size: 32 170 32 368 32 969 32 1633
Cuts at: 3553 3583 3585 3615 285
Size: 30 2 30 2381
Fragments arranged by size:
2381 1633 969 368 170 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 561 2750 561
Size: 2189 3522
KasI G'GCGC_C
Cuts at: 196 1494 1665 1968 3141 3389 196
Size: 1298 171 303 1173 248 2518
Fragments arranged by size:
2518 1298 1173 303 248 171
KpnI G_GTAC'C
Cuts at: 1677 2731 1677
Size: 1054 4657
MluI A'CGCG_T
Cuts at: 5144 5144
Size: 5711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 4382 4566 923
Size: 3459 184 2068
Fragments arranged by size:
3459 2068 184
MscI TGG'CCA
Cuts at: 2008 3472 2008
Size: 1464 4247
MslI CAynn'nnrTG
Cuts at: 730 1333 1558 2110 2305 2536 3700 3827 730
Size: 603 225 552 195 231 1164 127 2614
Fragments arranged by size:
2614 1164 603 552 231 225 195 127
MspA1I CmG'CkG
Cuts at: 136 290 345 462 756 974 1230 1454 1631
Size: 154 55 117 294 218 256 224 177
Cuts at: 1631 1671 1847 2019 2146 2220 2466 2628 2634
Size: 40 176 172 127 74 246 162 6
Cuts at: 2634 2649 2822 3136 3496 4509 4754 136
Size: 15 173 314 360 1013 245 1093
Fragments arranged by size:
1093 1013 360 314 294 256 246 245
224 218 177 176 173 172 162 154
127 117 74 55 40 15 6
NaeI GCC'GGC
Cuts at: 2579 3892 2579
Size: 1313 4398
NarI GG'CG_CC
Cuts at: 197 1495 1666 1969 3142 3390 197
Size: 1298 171 303 1173 248 2518
Fragments arranged by size:
2518 1298 1173 303 248 171
NcoI C'CATG_G
Cuts at: 107 2306 2423 107
Size: 2199 117 3395
Fragments arranged by size:
3395 2199 117
NgoMIV G'CCGG_C
Cuts at: 2577 3890 2577
Size: 1313 4398
NheI G'CTAG_C
Cuts at: 1146 1146
Size: 5711
NsiI A_TGCA'T
Cuts at: 697 697
Size: 5711
NspI r_CATG'y
Cuts at: 855 1059 1749 2764 3264 4171 855
Size: 204 690 1015 500 907 2395
Fragments arranged by size:
2395 1015 907 690 500 204
PasI CC'CwG_GG
Cuts at: 2400 2569 2400
Size: 169 5542
PciI A'CATG_T
Cuts at: 4167 4167
Size: 5711
PflMI CCAn_nnn'nTGG
Cuts at: 555 1722 2109 555
Size: 1167 387 4157
Fragments arranged by size:
4157 1167 387
PfoI T'CCnGG_A
Cuts at: 212 1568 1715 1829 1944 2500 212
Size: 1356 147 114 115 556 3423
Fragments arranged by size:
3423 1356 556 147 115 114
PmeI GTTT'AAAC
Cuts at: 2710 2710
Size: 5711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1864 1896 4850 4882 1864
Size: 32 2954 32 2693
Fragments arranged by size:
2954 2693 32 32
PpuMI rG'GwC_Cy
Cuts at: 330 621 717 1536 1719 2565 2601 330
Size: 291 96 819 183 846 36 3440
Fragments arranged by size:
3440 846 819 291 183 96 36
PspOMI G'GGCC_C
Cuts at: 145 2132 2158 2695 145
Size: 1987 26 537 3161
Fragments arranged by size:
3161 1987 537 26
PspXI vC'TCGA_Gb
Cuts at: 433 433
Size: 5711
PstI C_TGCA'G
Cuts at: 338 410 461 1551 2072 2413 2654 2758 3443
Size: 72 51 1090 521 341 241 104 685
Cuts at: 3443 338
Size: 2606
Fragments arranged by size:
2606 1090 685 521 341 241 104 72
51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5711
PvuII CAG'CTG
Cuts at: 290 345 462 756 974 1631 2634 2649 3136
Size: 55 117 294 218 657 1003 15 487
Cuts at: 3136 3496 290
Size: 360 2505
Fragments arranged by size:
2505 1003 657 487 360 294 218 117
55 15
RsrII CG'GwC_CG
Cuts at: 3906 3906
Size: 5711
SacI G_AGCT'C
Cuts at: 703 2509 2725 703
Size: 1806 216 3689
Fragments arranged by size:
3689 1806 216
SacII CC_GC'GG
Cuts at: 1455 1672 2147 1455
Size: 217 475 5019
Fragments arranged by size:
5019 475 217
SalI G'TCGA_C
Cuts at: 2748 2748
Size: 5711
SanDI GG'GwC_CC
Cuts at: 621 717 621
Size: 96 5615
SapI GCTCTTCn'nnn_
Cuts at: 1349 2377 3734 3944 1349
Size: 1028 1357 210 3116
Fragments arranged by size:
3116 1357 1028 210
SbfI CC_TGCA'GG
Cuts at: 2758 2758
Size: 5711
ScaI AGT'ACT
Cuts at: 5228 5228
Size: 5711
SfcI C'TryA_G
Cuts at: 33 334 406 457 1547 2068 2409 2650 2754
Size: 301 72 51 1090 521 341 241 104
Cuts at: 2754 3439 4432 4623 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1090 993 685 521 341 301 241
191 104 72 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 2005 2140 2005
Size: 135 5576
SfoI GGC'GCC
Cuts at: 198 1496 1667 1970 3143 3391 198
Size: 1298 171 303 1173 248 2518
Fragments arranged by size:
2518 1298 1173 303 248 171
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
SmaI CCC'GGG
Cuts at: 143 191 1793 2077 2234 2733 143
Size: 48 1602 284 157 499 3121
Fragments arranged by size:
3121 1602 499 284 157 48
SmlI C'TyrA_G
Cuts at: 433 2866 3781 4273 4535 433
Size: 2433 915 492 262 1609
Fragments arranged by size:
2433 1609 915 492 262
SnaBI TAC'GTA
Cuts at: 1605 1605
Size: 5711
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5711
SphI G_CATG'C
Cuts at: 1749 2764 3264 1749
Size: 1015 500 4196
Fragments arranged by size:
4196 1015 500
SrfI GCCC'GGGC
Cuts at: 1793 2234 1793
Size: 441 5270
StuI AGG'CCT
Cuts at: 1999 2212 1999
Size: 213 5498
StyI C'CwwG_G
Cuts at: 107 2289 2306 2423 2845 3199 107
Size: 2182 17 117 422 354 2619
Fragments arranged by size:
2619 2182 422 354 117 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 376 1056 1380 3294 3427 3886 4095 376
Size: 680 324 1914 133 459 209 1992
Fragments arranged by size:
1992 1914 680 459 324 209 133
TatI w'GTAC_w
Cuts at: 1070 1703 5226 1070
Size: 633 3523 1555
Fragments arranged by size:
3523 1555 633
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 880 912 1514 1546 2030 2062 5228 5260 880
Size: 32 602 32 484 32 3166 32 1331
Fragments arranged by size:
3166 1331 602 484 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1288 3508 1288
Size: 2220 3491
XbaI T'CTAG_A
Cuts at: 2742 2742
Size: 5711
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 729 1101 2016 3206 729
Size: 372 915 1190 3234
Fragments arranged by size:
3234 1190 915 372
XhoI C'TCGA_G
Cuts at: 433 433
Size: 5711
XmaI C'CCGG_G
Cuts at: 141 189 1791 2075 2232 2731 141
Size: 48 1602 284 157 499 3121
Fragments arranged by size:
3121 1602 499 284 157 48
XmnI GAAnn'nnTTC
Cuts at: 1043 1043
Size: 5711
ZraI GAC'GTC
Cuts at: 1644 1644
Size: 5711
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BsiWI BsmI BsrGI BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI PacI PmlI PshAI PsiI PsrI SexAI
SgrAI SspI SwaI