Restriction map for
pF1KB3720
(Circular) (Six-base) MAPSORT of: pF1KB3720.seq Check: 1861 from: 1 to: 4085
pF1KB3720 4085 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1123 1123
Size: 4085
Acc65I G'GTAC_C
Cuts at: 1101 1101
Size: 4085
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1910 2342 2695 3357 1910
Size: 432 353 662 2638
Fragments arranged by size:
2638 662 432 353
AflIII A'CryG_T
Cuts at: 1437 3237 3518 1437
Size: 1800 281 2004
Fragments arranged by size:
2004 1800 281
AleI CACnn'nnGTG
Cuts at: 272 853 272
Size: 581 3504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 278 278
Size: 4085
AlwNI CAG_nnn'CTG
Cuts at: 666 1390 2828 666
Size: 724 1438 1923
Fragments arranged by size:
1923 1438 724
ApaI G_GGCC'C
Cuts at: 1050 1050
Size: 4085
ApaLI G'TGCA_C
Cuts at: 2923 2923
Size: 4085
ApoI r'AATT_y
Cuts at: 484 1089 3438 3694 484
Size: 605 2349 256 875
Fragments arranged by size:
2349 875 605 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 743 1105 743
Size: 362 3723
AvrII C'CTAG_G
Cuts at: 1066 1066
Size: 4085
BamHI G'GATC_C
Cuts at: 1110 1110
Size: 4085
BanI G'GyrC_C
Cuts at: 847 950 1101 1515 1763 1798 847
Size: 103 151 414 248 35 3134
Fragments arranged by size:
3134 414 248 151 103 35
BanII G_rGCy'C
Cuts at: 985 1050 1099 2129 985
Size: 65 49 1030 2941
Fragments arranged by size:
2941 1030 65 49
BbeI G_GCGC'C
Cuts at: 1519 1767 1519
Size: 248 3837
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4051
BciVI GTATCCnnnnn_n'
Cuts at: 1983 3039 4085 1983
Size: 1056 1046 1983
Fragments arranged by size:
1983 1056 1046
BclI T'GATC_A
Cuts at: 1605 1605
Size: 4085
BglI GCCn_nnn'nGGC
Cuts at: 175 820 175
Size: 645 3440
BglII A'GATC_T
Cuts at: 1600 3637 1600
Size: 2037 2048
BlpI GC'TnA_GC
Cuts at: 1197 1197
Size: 4085
Bme1580I G_kGCm'C
Cuts at: 193 723 744 850 905 1050 1710 1803 2927
Size: 530 21 106 55 145 660 93 1124
Cuts at: 2927 193
Size: 1351
Fragments arranged by size:
1351 1124 660 530 145 106 93 55
21
BmgBI CAC'GTC
Cuts at: 962 962
Size: 4085
BmrI ACTGGGnnnn_n'
Cuts at: 453 1012 1297 1378 1713 3857 453
Size: 559 285 81 335 2144 681
Fragments arranged by size:
2144 681 559 335 285 81
BmtI G_CTAG'C
Cuts at: 365 775 365
Size: 410 3675
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 406 860 291
Size: 115 454 3516
Fragments arranged by size:
3516 454 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2140 2854 3152 1261
Size: 879 714 298 2194
Fragments arranged by size:
2194 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3789 3789
Size: 4085
BsaAI yAC'GTr
Cuts at: 729 1438 729
Size: 709 3376
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 1516 1764 2466 3962 1516
Size: 248 702 1496 1639
Fragments arranged by size:
1639 1496 702 248
BsaWI w'CCGG_w
Cuts at: 242 1016 1274 1795 2473 2884 3031 3305 242
Size: 774 258 521 678 411 147 274 1022
Fragments arranged by size:
1022 774 678 521 411 274 258 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 577 607 577
Size: 30 4055
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 359 823 331
Size: 28 464 3593
Fragments arranged by size:
3593 464 28
BseYI C'CCAG_C
Cuts at: 652 670 702 1043 1510 2268 2933 3341 652
Size: 18 32 341 467 758 665 408 1396
Fragments arranged by size:
1396 758 665 467 408 341 32 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 315 248
Size: 67 4018
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2903 104
Size: 1523 46 1230 1286
Fragments arranged by size:
1523 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1099 1877 2067 2927 1099
Size: 778 190 860 2257
Fragments arranged by size:
2257 860 778 190
BsmI GAATG_Cn'
Cuts at: 529 529
Size: 4085
Bsp1286I G_dGCh'C
Cuts at: 193 723 744 850 905 985 1050 1099 1710
Size: 530 21 106 55 80 65 49 611
Cuts at: 1710 1803 1877 2067 2129 2927 193
Size: 93 74 190 62 798 1351
Fragments arranged by size:
1351 798 611 530 190 106 93 80
74 65 62 55 49 21
BspEI T'CCGG_A
Cuts at: 1016 1016
Size: 4085
BspHI T'CATG_A
Cuts at: 2517 2517
Size: 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1135 1349 1651 2032 1135
Size: 214 302 381 3188
Fragments arranged by size:
3188 381 302 214
BsrBI CCG'CTC
Cuts at: 50 588 2377 2431 3915 4080 50
Size: 538 1789 54 1484 165 55
Fragments arranged by size:
1789 1484 538 165 55 54
BsrDI GCAATG_nn'
Cuts at: 926 1997 926
Size: 1071 3014
BsrFI r'CCGG_y
Cuts at: 2083 2264 2083
Size: 181 3904
BssHII G'CGCG_C
Cuts at: 119 119
Size: 4085
BssSI C'ACGA_G
Cuts at: 2356 3064 2356
Size: 708 3377
BstAPI GCAn_nnn'nTGC
Cuts at: 1462 1462
Size: 4085
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstXI CCAn_nnnn'nTGG
Cuts at: 138 604 138
Size: 466 3619
BstYI r'GATC_y
Cuts at: 1 1110 1577 1600 1935 2181 2487 2499 2585
Size: 1109 467 23 335 246 306 12 86
Cuts at: 2585 3637 1
Size: 1052 449
Fragments arranged by size:
1109 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 452 642 905 452
Size: 190 263 3632
Fragments arranged by size:
3632 263 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 546 2035 2216 2379 546
Size: 1489 181 163 2252
Fragments arranged by size:
2252 1489 181 163
BtsI GCAGTG_nn'
Cuts at: 327 1328 327
Size: 1001 3084
DraI TTT'AAA
Cuts at: 1084 1084
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 1791 3135 1791
Size: 1344 2741
EaeI y'GGCC_r
Cuts at: 1670 1844 2235 2262 3607 1670
Size: 174 391 27 1345 2148
Fragments arranged by size:
2148 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2293 3035 3181 3895 2293
Size: 742 146 714 2483
Fragments arranged by size:
2483 742 714 146
EcoICRI GAG'CTC
Cuts at: 1097 1097
Size: 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 406 860 1910 2342 2695 3357 291
Size: 115 454 1050 432 353 662 1019
Fragments arranged by size:
1050 1019 662 454 432 353 115
EcoO109I rG'GnC_Cy
Cuts at: 663 822 1030 1224 663
Size: 159 208 194 3524
Fragments arranged by size:
3524 208 194 159
EcoRI G'AATT_C
Cuts at: 1089 1089
Size: 4085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 4085
FspI TGC'GCA
Cuts at: 397 1866 397
Size: 1469 2616
HaeII r_GCGC'y
Cuts at: 1519 1767 2997 3768 1519
Size: 248 1230 771 1836
Fragments arranged by size:
1836 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 599 631 1927 1957 1959 1989 291
Size: 32 276 32 1296 30 2 30 2387
Fragments arranged by size:
2387 1296 276 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1124 1124
Size: 4085
KasI G'GCGC_C
Cuts at: 1515 1763 1515
Size: 248 3837
KpnI G_GTAC'C
Cuts at: 1105 1105
Size: 4085
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 575 2844 3028 575
Size: 2269 184 1632
Fragments arranged by size:
2269 1632 184
MscI TGG'CCA
Cuts at: 1846 1846
Size: 4085
MslI CAynn'nnrTG
Cuts at: 272 577 853 2074 2201 272
Size: 305 276 1221 127 2156
Fragments arranged by size:
2156 1221 305 276 127
MspA1I CmG'CkG
Cuts at: 947 1196 1510 1870 2654 2899 947
Size: 249 314 360 784 245 2133
Fragments arranged by size:
2133 784 360 314 249 245
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 4085
NarI GG'CG_CC
Cuts at: 1516 1764 1516
Size: 248 3837
NcoI C'CATG_G
Cuts at: 642 642
Size: 4085
NdeI CA'TA_TG
Cuts at: 804 917 804
Size: 113 3972
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 4085
NheI G'CTAG_C
Cuts at: 361 771 361
Size: 410 3675
NspI r_CATG'y
Cuts at: 1138 1638 3241 1138
Size: 500 1603 1982
Fragments arranged by size:
1982 1603 500
PciI A'CATG_T
Cuts at: 3237 3237
Size: 4085
PflMI CCAn_nnn'nTGG
Cuts at: 474 474
Size: 4085
PfoI T'CCnGG_A
Cuts at: 752 752
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 4053
PpuMI rG'GwC_Cy
Cuts at: 1030 1030
Size: 4085
PspOMI G'GGCC_C
Cuts at: 1046 1046
Size: 4085
PstI C_TGCA'G
Cuts at: 662 785 1132 1817 662
Size: 123 347 685 2930
Fragments arranged by size:
2930 685 347 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 947 1510 1870 947
Size: 563 360 3162
Fragments arranged by size:
3162 563 360
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 1099 1099
Size: 4085
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
SbfI CC_TGCA'GG
Cuts at: 662 1132 662
Size: 470 3615
ScaI AGT'ACT
Cuts at: 403 3602 403
Size: 3199 886
SexAI A'CCwGG_T
Cuts at: 215 215
Size: 4085
SfcI C'TryA_G
Cuts at: 33 658 781 942 1128 1813 2781 2972 33
Size: 625 123 161 186 685 968 191 1146
Fragments arranged by size:
1146 968 685 625 191 186 161 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 820 820
Size: 4085
SfoI GGC'GCC
Cuts at: 1517 1765 1517
Size: 248 3837
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 1107 1107
Size: 4085
SmlI C'TyrA_G
Cuts at: 1240 2155 2869 3131 1240
Size: 915 714 262 2194
Fragments arranged by size:
2194 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 1138 1638 1138
Size: 500 3585
StyI C'CwwG_G
Cuts at: 489 642 851 1066 1219 1573 489
Size: 153 209 215 153 354 3001
Fragments arranged by size:
3001 354 215 209 153 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 993 1668 1801 2260 2469 993
Size: 675 133 459 209 2609
Fragments arranged by size:
2609 675 459 209 133
TatI w'GTAC_w
Cuts at: 279 401 3600 279
Size: 122 3199 764
Fragments arranged by size:
3199 764 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 433 465 577 609 3602 3634 433
Size: 32 112 32 2993 32 884
Fragments arranged by size:
2993 884 112 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 999 1580 999
Size: 581 3504
XmaI C'CCGG_G
Cuts at: 1105 1105
Size: 4085
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
BaeI BbsI BbvCI BfrBI Bpu10I BsiWI BsmBI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI