Restriction map for
pF1KB3725
(Circular) (Six-base) MAPSORT of: pF1KB3725.seq Check: 4958 from: 1 to: 7238
pF1KB3725 7238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 776 1902 2189 4276 776
Size: 1126 287 2087 3738
Fragments arranged by size:
3738 2087 1126 287
Acc65I G'GTAC_C
Cuts at: 1634 2629 3012 4254 1634
Size: 995 383 1242 4618
Fragments arranged by size:
4618 1242 995 383
AclI AA'CG_TT
Cuts at: 7078 7078
Size: 7238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1250 1639 2323 3233 3362 3982 4055 4127
Size: 538 389 684 910 129 620 73 72
Cuts at: 4127 5063 5495 6242 6510 712
Size: 936 432 747 268 1440
Fragments arranged by size:
1440 936 910 747 684 620 538 432
389 268 129 73 72
AflII C'TTAA_G
Cuts at: 3967 3967
Size: 7238
AflIII A'CryG_T
Cuts at: 4144 4590 5694 6671 4144
Size: 446 1104 977 4711
Fragments arranged by size:
4711 1104 977 446
AgeI A'CCGG_T
Cuts at: 3824 5626 3824
Size: 1802 5436
AhdI GACnn_n'nnGTC
Cuts at: 2700 2700
Size: 7238
AlwNI CAG_nnn'CTG
Cuts at: 269 690 2029 2271 2328 2875 4543 6110 269
Size: 421 1339 242 57 547 1668 1567 1397
Fragments arranged by size:
1668 1567 1397 1339 547 421 242 57
ApaLI G'TGCA_C
Cuts at: 723 4188 6008 723
Size: 3465 1820 1953
Fragments arranged by size:
3465 1953 1820
ApoI r'AATT_y
Cuts at: 1001 1914 1954 3146 3180 3397 3986 4014 4242
Size: 913 40 1192 34 217 589 28 228
Cuts at: 4242 6591 6847 1001
Size: 2349 256 1392
Fragments arranged by size:
2349 1392 1192 913 589 256 228 217
40 34 28
AseI AT'TA_AT
Cuts at: 20 1406 1792 2540 20
Size: 1386 386 748 4718
Fragments arranged by size:
4718 1386 748 386
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7238
AvaI C'yCGr_G
Cuts at: 162 181 324 338 4258 162
Size: 19 143 14 3920 3142
Fragments arranged by size:
3920 3142 143 19 14
BamHI G'GATC_C
Cuts at: 3278 4263 3278
Size: 985 6253
BanI G'GyrC_C
Cuts at: 478 1634 2629 2646 3012 4254 4668 4916 4951
Size: 1156 995 17 366 1242 414 248 35
Cuts at: 4951 478
Size: 2765
Fragments arranged by size:
2765 1242 1156 995 414 366 248 35
17
BanII G_rGCy'C
Cuts at: 818 1463 2335 4252 5282 818
Size: 645 872 1917 1030 2774
Fragments arranged by size:
2774 1917 1030 872 645
BbeI G_GCGC'C
Cuts at: 482 4672 4920 482
Size: 4190 248 2800
Fragments arranged by size:
4190 2800 248
BbsI GAAGACnn'nnnn_
Cuts at: 1483 1627 3296 3686 3741 4088 1483
Size: 144 1669 390 55 347 4633
Fragments arranged by size:
4633 1669 390 347 144 55
BbvCI CC'TCA_GC
Cuts at: 3241 3241
Size: 7238
BciVI GTATCCnnnnn_n'
Cuts at: 5136 5897 7238 5136
Size: 761 1341 5136
Fragments arranged by size:
5136 1341 761
BclI T'GATC_A
Cuts at: 395 3672 4758 395
Size: 3277 1086 2875
Fragments arranged by size:
3277 2875 1086
BfrBI ATG'CAT
Cuts at: 1174 1174
Size: 7238
BglII A'GATC_T
Cuts at: 2564 4753 6790 2564
Size: 2189 2037 3012
Fragments arranged by size:
3012 2189 2037
BlpI GC'TnA_GC
Cuts at: 4350 4350
Size: 7238
Bme1580I G_kGCm'C
Cuts at: 727 4192 4863 4956 6012 727
Size: 3465 671 93 1056 1953
Fragments arranged by size:
3465 1953 1056 671 93
BmrI ACTGGGnnnn_n'
Cuts at: 737 1542 2767 4450 4531 4866 7010 737
Size: 805 1225 1683 81 335 2144 965
Fragments arranged by size:
2144 1683 1225 965 805 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3072 5605 3072
Size: 2533 4705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2140 2402 2620 3475 1507
Size: 633 262 218 855 5270
Fragments arranged by size:
5270 855 633 262 218
Bpu10I CC'TnA_GC
Cuts at: 2954 3241 3332 2954
Size: 287 91 6860
Fragments arranged by size:
6860 287 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2226 2545 4185 4414 5293 5785 6083 1624
Size: 602 319 1640 229 879 492 298 2779
Fragments arranged by size:
2779 1640 879 602 492 319 298 229
BsaI GGTCTCn'nnnn_
Cuts at: 1337 2708 6942 1337
Size: 1371 4234 1633
Fragments arranged by size:
4234 1633 1371
BsaAI yAC'GTr
Cuts at: 1878 4147 4591 1878
Size: 2269 444 4525
Fragments arranged by size:
4525 2269 444
BsaBI GATnn'nnATC
Cuts at: 6 4752 6789 6
Size: 4746 2037 455
Fragments arranged by size:
4746 2037 455
BsaHI Gr'CG_yC
Cuts at: 479 4669 4917 5619 7115 479
Size: 4190 248 702 1496 602
Fragments arranged by size:
4190 1496 702 602 248
BsaWI w'CCGG_w
Cuts at: 1463 3824 4427 4948 5626 5900 6047 6458 1463
Size: 2361 603 521 678 274 147 411 2243
Fragments arranged by size:
2361 2243 678 603 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3899 3929 3899
Size: 30 7208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 221 247 312 1411 3191 221
Size: 26 65 1099 1780 4268
Fragments arranged by size:
4268 1780 1099 65 26
BseYI C'CCAG_C
Cuts at: 4663 5421 5998 6494 4663
Size: 758 577 496 5407
Fragments arranged by size:
5407 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 156
Size: 7238
BsiEI CG_ry'CG
Cuts at: 104 4780 4826 6034 104
Size: 4676 46 1208 1308
Fragments arranged by size:
4676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 239 727 818 1463 3201 3784 3822 4192
Size: 124 488 91 645 1738 583 38 370
Cuts at: 4192 4252 5030 5220 6012 115
Size: 60 778 190 792 1341
Fragments arranged by size:
1738 1341 792 778 645 583 488 370
190 124 91 60 38
BsmI GAATG_Cn'
Cuts at: 2845 3167 2845
Size: 322 6916
Bsp1286I G_dGCh'C
Cuts at: 115 239 727 818 1463 2335 3201 3784 3822
Size: 124 488 91 645 872 866 583 38
Cuts at: 3822 4192 4252 4863 4956 5030 5220 5282 6012
Size: 370 60 611 93 74 190 62 730
Cuts at: 6012 115
Size: 1341
Fragments arranged by size:
1341 872 866 730 645 611 583 488
370 190 124 93 91 74 62 60
38
BspEI T'CCGG_A
Cuts at: 1463 6458 1463
Size: 4995 2243
BspHI T'CATG_A
Cuts at: 797 3804 6414 797
Size: 3007 2610 1621
Fragments arranged by size:
3007 2610 1621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2185 2364 4288 4502 4804 5185 2185
Size: 179 1924 214 302 381 4238
Fragments arranged by size:
4238 1924 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 211 5530 5584 7068 7233 50
Size: 161 5319 54 1484 165 55
Fragments arranged by size:
5319 1484 165 161 55 54
BsrDI GCAATG_nn'
Cuts at: 2287 3380 5150 2287
Size: 1093 1770 4375
Fragments arranged by size:
4375 1770 1093
BsrFI r'CCGG_y
Cuts at: 3009 3824 4094 5236 5417 5626 3009
Size: 815 270 1142 181 209 4621
Fragments arranged by size:
4621 1142 815 270 209 181
BsrGI T'GTAC_A
Cuts at: 2020 2713 3837 2020
Size: 693 1124 5421
Fragments arranged by size:
5421 1124 693
BssSI C'ACGA_G
Cuts at: 5509 5867 5509
Size: 358 6880
BstAPI GCAn_nnn'nTGC
Cuts at: 243 453 559 4615 243
Size: 210 106 4056 2866
Fragments arranged by size:
4056 2866 210 106
BstBI TT'CG_AA
Cuts at: 4218 5599 4218
Size: 1381 5857
BstXI CCAn_nnnn'nTGG
Cuts at: 534 534
Size: 7238
BstYI r'GATC_y
Cuts at: 1 2564 2813 2871 3278 3406 4263 4730 4753
Size: 2563 249 58 407 128 857 467 23
Cuts at: 4753 5088 5334 6346 6432 6444 6790 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2563 1012 857 467 449 407 346 335
249 246 128 86 58 23 12
BstZ17I GTA'TAC
Cuts at: 1903 1903
Size: 7238
Bsu36I CC'TnA_GG
Cuts at: 3740 3771 3740
Size: 31 7207
BtgI C'CryG_G
Cuts at: 380 1238 1347 3474 380
Size: 858 109 2127 4144
Fragments arranged by size:
4144 2127 858 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 593 5188 5369 5532 593
Size: 4595 181 163 2299
Fragments arranged by size:
4595 2299 181 163
BtsI GCAGTG_nn'
Cuts at: 336 3051 4481 336
Size: 2715 1430 3093
Fragments arranged by size:
3093 2715 1430
DraI TTT'AAA
Cuts at: 1614 2799 2921 4237 1614
Size: 1185 122 1316 4615
Fragments arranged by size:
4615 1316 1185 122
DraIII CAC_nnn'GTG
Cuts at: 1158 4196 1158
Size: 3038 4200
DrdI GACnn_nn'nnGTC
Cuts at: 4944 5802 4944
Size: 858 6380
EaeI y'GGCC_r
Cuts at: 314 428 638 1706 2729 4823 4997 5388 5415
Size: 114 210 1068 1023 2094 174 391 27
Cuts at: 5415 6760 314
Size: 1345 792
Fragments arranged by size:
2094 1345 1068 1023 792 391 210 174
114 27
EagI C'GGCC_G
Cuts at: 4823 4823
Size: 7238
EarI CTCTTCn'nnn_
Cuts at: 1932 2066 5261 5471 1932
Size: 134 3195 210 3699
Fragments arranged by size:
3699 3195 210 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 5446 5756 5902 7048 136
Size: 5310 310 146 1146 326
Fragments arranged by size:
5310 1146 326 310 146
EcoICRI GAG'CTC
Cuts at: 816 1461 4250 816
Size: 645 2789 3804
Fragments arranged by size:
3804 2789 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1250 1507 1639 2140 2323 2402 2620 3233
Size: 538 257 132 501 183 79 218 613
Cuts at: 3233 3362 3475 3982 4055 4127 5063 5495 6242
Size: 129 113 507 73 72 936 432 747
Cuts at: 6242 6510 712
Size: 268 1440
Fragments arranged by size:
1440 936 747 613 538 507 501 432
268 257 218 183 132 129 113 79
73 72
EcoNI CCTnn'n_nnAGG
Cuts at: 1747 1747
Size: 7238
EcoO109I rG'GnC_Cy
Cuts at: 441 2376 4377 441
Size: 1935 2001 3302
Fragments arranged by size:
3302 2001 1935
EcoRI G'AATT_C
Cuts at: 1001 3146 4242 1001
Size: 2145 1096 3997
Fragments arranged by size:
3997 2145 1096
EcoRV GAT'ATC
Cuts at: 1077 1077
Size: 7238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1527 3934 5257 1527
Size: 2407 1323 3508
Fragments arranged by size:
3508 2407 1323
FspI TGC'GCA
Cuts at: 5019 5019
Size: 7238
HaeII r_GCGC'y
Cuts at: 287 482 4672 4920 5942 6921 287
Size: 195 4190 248 1022 979 604
Fragments arranged by size:
4190 1022 979 604 248 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 1129 1161 2686 2718 2812 2844 3082
Size: 32 442 32 1525 32 94 32 238
Cuts at: 3082 3114 5080 5110 5112 5142 655
Size: 32 1966 30 2 30 2751
Fragments arranged by size:
2751 1966 1525 442 238 94 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 809 4277 809
Size: 3468 3770
HindIII A'AGCT_T
Cuts at: 1606 2956 3964 1606
Size: 1350 1008 4880
Fragments arranged by size:
4880 1350 1008
KasI G'GCGC_C
Cuts at: 478 4668 4916 478
Size: 4190 248 2800
Fragments arranged by size:
4190 2800 248
KpnI G_GTAC'C
Cuts at: 1638 2633 3016 4258 1638
Size: 995 383 1242 4618
Fragments arranged by size:
4618 1242 995 383
MfeI C'AATT_G
Cuts at: 2444 2444
Size: 7238
MluI A'CGCG_T
Cuts at: 6671 6671
Size: 7238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1521 5909 6093 1521
Size: 4388 184 2666
Fragments arranged by size:
4388 2666 184
MscI TGG'CCA
Cuts at: 640 1708 2731 4999 640
Size: 1068 1023 2268 2879
Fragments arranged by size:
2879 2268 1068 1023
MslI CAynn'nnrTG
Cuts at: 1154 1629 2725 5227 5354 1154
Size: 475 1096 2502 127 3038
Fragments arranged by size:
3038 2502 1096 475 127
MspA1I CmG'CkG
Cuts at: 243 289 988 1432 2974 4349 4663 5023 6036
Size: 46 699 444 1542 1375 314 360 1013
Cuts at: 6036 6281 243
Size: 245 1200
Fragments arranged by size:
1542 1375 1200 1013 699 444 360 314
245 46
NaeI GCC'GGC
Cuts at: 5419 5419
Size: 7238
NarI GG'CG_CC
Cuts at: 479 4669 4917 479
Size: 4190 248 2800
Fragments arranged by size:
4190 2800 248
NcoI C'CATG_G
Cuts at: 1238 1347 3474 1238
Size: 109 2127 5002
Fragments arranged by size:
5002 2127 109
NdeI CA'TA_TG
Cuts at: 390 390
Size: 7238
NgoMIV G'CCGG_C
Cuts at: 5417 5417
Size: 7238
NsiI A_TGCA'T
Cuts at: 1176 1176
Size: 7238
NspI r_CATG'y
Cuts at: 4291 4791 5698 4291
Size: 500 907 5831
Fragments arranged by size:
5831 907 500
PciI A'CATG_T
Cuts at: 5694 5694
Size: 7238
PflMI CCAn_nnn'nTGG
Cuts at: 2328 2643 2328
Size: 315 6923
PfoI T'CCnGG_A
Cuts at: 923 1079 923
Size: 156 7082
PmeI GTTT'AAAC
Cuts at: 4237 4237
Size: 7238
PmlI CAC'GTG
Cuts at: 1878 4147 1878
Size: 2269 4969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6377 6409 6377
Size: 32 7206
PpuMI rG'GwC_Cy
Cuts at: 2376 2376
Size: 7238
PspXI vC'TCGA_Gb
Cuts at: 324 324
Size: 7238
PstI C_TGCA'G
Cuts at: 199 449 686 2835 4285 4970 199
Size: 250 237 2149 1450 685 2467
Fragments arranged by size:
2467 2149 1450 685 250 237
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7238
PvuII CAG'CTG
Cuts at: 243 988 1432 2974 4663 5023 243
Size: 745 444 1542 1689 360 2458
Fragments arranged by size:
2458 1689 1542 745 444 360
RsrII CG'GwC_CG
Cuts at: 5433 5433
Size: 7238
SacI G_AGCT'C
Cuts at: 818 1463 4252 818
Size: 645 2789 3804
Fragments arranged by size:
3804 2789 645
SalI G'TCGA_C
Cuts at: 4275 4275
Size: 7238
SapI GCTCTTCn'nnn_
Cuts at: 5261 5471 5261
Size: 210 7028
SbfI CC_TGCA'GG
Cuts at: 4285 4285
Size: 7238
ScaI AGT'ACT
Cuts at: 919 6755 919
Size: 5836 1402
SfcI C'TryA_G
Cuts at: 33 195 445 682 1068 1393 2547 2831 4281
Size: 162 250 237 386 325 1154 284 1450
Cuts at: 4281 4966 5959 6150 33
Size: 685 993 191 1121
Fragments arranged by size:
1450 1154 1121 993 685 386 325 284
250 237 191 162
SfoI GGC'GCC
Cuts at: 480 4670 4918 480
Size: 4190 248 2800
Fragments arranged by size:
4190 2800 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7238
SmaI CCC'GGG
Cuts at: 4260 4260
Size: 7238
SmlI C'TyrA_G
Cuts at: 324 1639 2205 2560 3967 4164 4393 5308 5800
Size: 1315 566 355 1407 197 229 915 492
Cuts at: 5800 6062 324
Size: 262 1500
Fragments arranged by size:
1500 1407 1315 915 566 492 355 262
229 197
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7238
SphI G_CATG'C
Cuts at: 4291 4791 4291
Size: 500 6738
SspI AAT'ATT
Cuts at: 1796 2316 1796
Size: 520 6718
StyI C'CwwG_G
Cuts at: 411 612 1238 1347 2840 3474 4372 4726 411
Size: 201 626 109 1493 634 898 354 2923
Fragments arranged by size:
2923 1493 898 634 626 354 201 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3431 4821 4954 5413 5622 3431
Size: 1390 133 459 209 5047
Fragments arranged by size:
5047 1390 459 209 133
TatI w'GTAC_w
Cuts at: 917 2020 2611 2713 3837 6753 917
Size: 1103 591 102 1124 2916 1402
Fragments arranged by size:
2916 1402 1124 1103 591 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 729 761 1905 1937 3237 3269 6755 6787 729
Size: 32 1144 32 1300 32 3486 32 1180
Fragments arranged by size:
3486 1300 1180 1144 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2155 5035 2155
Size: 2880 4358
XbaI T'CTAG_A
Cuts at: 4269 4269
Size: 7238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3615 3873 4733 3615
Size: 258 860 6120
Fragments arranged by size:
6120 860 258
XhoI C'TCGA_G
Cuts at: 324 324
Size: 7238
XmaI C'CCGG_G
Cuts at: 4258 4258
Size: 7238
XmnI GAAnn'nnTTC
Cuts at: 2350 2391 3887 2350
Size: 41 1496 5701
Fragments arranged by size:
5701 1496 41
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI
BssHII BstEII ClaI CspCI FseI FspAI HpaI NheI
NotI NruI PacI PasI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI ZraI