Restriction map for
pF1KB3734
(Circular) (Six-base) MAPSORT of: pF1KB3734.seq Check: 6461 from: 1 to: 4496
pF1KB3734 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 271 1402 2321 2753 3106 3768 230
Size: 41 1131 919 432 353 662 958
Fragments arranged by size:
1131 958 919 662 432 353 41
AflIII A'CryG_T
Cuts at: 1848 3648 3929 1848
Size: 1800 281 2415
Fragments arranged by size:
2415 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1801 3239 1801
Size: 1438 3058
ApaLI G'TGCA_C
Cuts at: 3334 3334
Size: 4496
ApoI r'AATT_y
Cuts at: 1500 3849 4105 1500
Size: 2349 256 1891
Fragments arranged by size:
2349 1891 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1337 1516 1337
Size: 179 4317
BamHI G'GATC_C
Cuts at: 204 1521 204
Size: 1317 3179
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 515 1361 1510 2540 515
Size: 846 149 1030 2471
Fragments arranged by size:
2471 1030 846 149
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3450 4496 2394
Size: 1056 1046 2394
Fragments arranged by size:
2394 1056 1046
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglII A'GATC_T
Cuts at: 449 2011 4048 449
Size: 1562 2037 897
Fragments arranged by size:
2037 1562 897
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 2121 2214 3338 2121
Size: 93 1124 3279
Fragments arranged by size:
3279 1124 93
BmgBI CAC'GTC
Cuts at: 904 904
Size: 4496
BmrI ACTGGGnnnn_n'
Cuts at: 315 577 1708 1789 2124 4268 315
Size: 262 1131 81 335 2144 543
Fragments arranged by size:
2144 1131 543 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 194 171
Size: 23 4473
Bpu10I CC'TnA_GC
Cuts at: 233 233
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3265 3563 1672
Size: 879 714 298 2605
Fragments arranged by size:
2605 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 1196 2010 4047 6
Size: 1190 814 2037 455
Fragments arranged by size:
2037 1190 814 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3295 3442 3716 1685
Size: 521 678 411 147 274 2465
Fragments arranged by size:
2465 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1417 1447 1417
Size: 30 4466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 340
Size: 4496
BseYI C'CCAG_C
Cuts at: 1095 1921 2679 3344 3752 1095
Size: 826 758 665 408 1839
Fragments arranged by size:
1839 826 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3314 104
Size: 1934 46 1230 1286
Fragments arranged by size:
1934 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 515 1397 1510 2288 2478 3338 515
Size: 882 113 778 190 860 1673
Fragments arranged by size:
1673 882 860 778 190 113
BsmI GAATG_Cn'
Cuts at: 1243 1243
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 515 1361 1397 1510 2121 2214 2288 2478 2540
Size: 846 36 113 611 93 74 190 62
Cuts at: 2540 3338 515
Size: 798 1673
Fragments arranged by size:
1673 846 798 611 190 113 93 74
62 36
BspHI T'CATG_A
Cuts at: 918 2928 918
Size: 2010 2486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1163 1546 1760 2062 2443 1163
Size: 383 214 302 381 3216
Fragments arranged by size:
3216 383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2408 2408
Size: 4496
BsrFI r'CCGG_y
Cuts at: 2494 2675 2494
Size: 181 4315
BsrGI T'GTAC_A
Cuts at: 110 1370 110
Size: 1260 3236
BssSI C'ACGA_G
Cuts at: 2767 3475 2767
Size: 708 3788
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstYI r'GATC_y
Cuts at: 1 204 449 1028 1521 1988 2011 2346 2592
Size: 203 245 579 493 467 23 335 246
Cuts at: 2592 2898 2910 2996 4048 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 579 493 467 449 335 306 246
245 203 86 23 12
BtgI C'CryG_G
Cuts at: 503 503
Size: 4496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 152 2446 2627 2790 152
Size: 2294 181 163 1858
Fragments arranged by size:
2294 1858 181 163
BtsI GCAGTG_nn'
Cuts at: 799 1331 1739 799
Size: 532 408 3556
Fragments arranged by size:
3556 532 408
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DraIII CAC_nnn'GTG
Cuts at: 1320 1320
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3546 2202
Size: 1344 3152
EaeI y'GGCC_r
Cuts at: 1295 2081 2255 2646 2673 4018 1295
Size: 786 174 391 27 1345 1773
Fragments arranged by size:
1773 1345 786 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 221 290 673 736 782 2519 2729 221
Size: 69 383 63 46 1737 210 1988
Fragments arranged by size:
1988 1737 383 210 69 63 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3446 3592 4306 2704
Size: 742 146 714 2894
Fragments arranged by size:
2894 742 714 146
EcoICRI GAG'CTC
Cuts at: 513 1508 513
Size: 995 3501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 194 230 271 1402 2321 2753 3106 3768
Size: 23 36 41 1131 919 432 353 662
Cuts at: 3768 171
Size: 899
Fragments arranged by size:
1131 919 899 662 432 353 41 36
23
EcoO109I rG'GnC_Cy
Cuts at: 362 1635 362
Size: 1273 3223
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
EcoRV GAT'ATC
Cuts at: 1194 1194
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1930 2178 3408 4179 1930
Size: 248 1230 771 2247
Fragments arranged by size:
2247 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1020 1052 2338 2368 2370 2400 1020
Size: 32 1286 30 2 30 3116
Fragments arranged by size:
3116 1286 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
HindIII A'AGCT_T
Cuts at: 758 959 758
Size: 201 4295
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3255 3439 3255
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 461 1166 2485 2612 461
Size: 705 1319 127 2345
Fragments arranged by size:
2345 1319 705 127
MspA1I CmG'CkG
Cuts at: 1020 1607 1921 2281 3065 3310 1020
Size: 587 314 360 784 245 2206
Fragments arranged by size:
2206 784 587 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NspI r_CATG'y
Cuts at: 1549 2049 3652 1549
Size: 500 1603 2393
Fragments arranged by size:
2393 1603 500
PciI A'CATG_T
Cuts at: 3648 3648
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 362 362
Size: 4496
PstI C_TGCA'G
Cuts at: 1543 2228 1543
Size: 685 3811
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1020 1921 2281 1020
Size: 901 360 3235
Fragments arranged by size:
3235 901 360
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 515 1510 515
Size: 995 3501
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 221 782 2519 2729 221
Size: 561 1737 210 1988
Fragments arranged by size:
1988 1737 561 210
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SexAI A'CCwGG_T
Cuts at: 1177 1177
Size: 4496
SfcI C'TryA_G
Cuts at: 33 1326 1539 2224 3192 3383 33
Size: 1293 213 685 968 191 1146
Fragments arranged by size:
1293 1146 968 685 213 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1651 2566 3280 3542 1651
Size: 915 714 262 2605
Fragments arranged by size:
2605 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
SspI AAT'ATT
Cuts at: 627 782 627
Size: 155 4341
StyI C'CwwG_G
Cuts at: 1415 1630 1984 1415
Size: 215 354 3927
Fragments arranged by size:
3927 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 2079 2212 2671 2880 898
Size: 1181 133 459 209 2514
Fragments arranged by size:
2514 1181 459 209 133
TatI w'GTAC_w
Cuts at: 110 548 1203 1370 4011 110
Size: 438 655 167 2641 595
Fragments arranged by size:
2641 655 595 438 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1417 1449 4013 4045 1417
Size: 32 2564 32 1868
Fragments arranged by size:
2564 1868 32 32
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 936 1527 936
Size: 591 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1991 1991
Size: 4496
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmtI BsgI BsiWI BsmBI BspEI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI