Restriction map for
pF1KB3755
(Circular) (Six-base) MAPSORT of: pF1KB3755.seq Check: 6080 from: 1 to: 4883
pF1KB3755 4883 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1266 1266
Size: 4883
AatII G_ACGT'C
Cuts at: 1436 1436
Size: 4883
AccI GT'mk_AC
Cuts at: 1921 1921
Size: 4883
Acc65I G'GTAC_C
Cuts at: 268 1899 268
Size: 1631 3252
AclI AA'CG_TT
Cuts at: 4723 4723
Size: 4883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 1534 2708 3140 3887 4155 424
Size: 1110 1174 432 747 268 1152
Fragments arranged by size:
1174 1152 1110 747 432 268
AflII C'TTAA_G
Cuts at: 429 429
Size: 4883
AflIII A'CryG_T
Cuts at: 2235 3339 4316 2235
Size: 1104 977 2802
Fragments arranged by size:
2802 1104 977
AgeI A'CCGG_T
Cuts at: 3271 3271
Size: 4883
AhdI GACnn_n'nnGTC
Cuts at: 1523 1840 1523
Size: 317 4566
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 741 773 1141 1173 741
Size: 32 368 32 4451
Fragments arranged by size:
4451 368 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2188 3755 2188
Size: 1567 3316
ApaLI G'TGCA_C
Cuts at: 3653 3653
Size: 4883
ApoI r'AATT_y
Cuts at: 1887 4236 4492 1887
Size: 2349 256 2278
Fragments arranged by size:
2349 2278 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
AvaI C'yCGr_G
Cuts at: 198 236 1864 1903 198
Size: 38 1628 39 3178
Fragments arranged by size:
3178 1628 39 38
BamHI G'GATC_C
Cuts at: 1908 1908
Size: 4883
BanI G'GyrC_C
Cuts at: 268 1278 1899 2313 2561 2596 268
Size: 1010 621 414 248 35 2555
Fragments arranged by size:
2555 1010 621 414 248 35
BanII G_rGCy'C
Cuts at: 711 794 1897 2927 711
Size: 83 1103 1030 2667
Fragments arranged by size:
2667 1103 1030 83
BbeI G_GCGC'C
Cuts at: 2317 2565 2317
Size: 248 4635
BbsI GAAGACnn'nnnn_
Cuts at: 306 412 1522 306
Size: 106 1110 3667
Fragments arranged by size:
3667 1110 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 798 832 798
Size: 34 4849
BciVI GTATCCnnnnn_n'
Cuts at: 161 2781 3542 4883 161
Size: 2620 761 1341 161
Fragments arranged by size:
2620 1341 761 161
BclI T'GATC_A
Cuts at: 2403 2403
Size: 4883
BglI GCCn_nnn'nGGC
Cuts at: 1495 1495
Size: 4883
BglII A'GATC_T
Cuts at: 2398 4435 2398
Size: 2037 2846
BlpI GC'TnA_GC
Cuts at: 1995 1995
Size: 4883
Bme1580I G_kGCm'C
Cuts at: 2508 2601 3657 2508
Size: 93 1056 3734
Fragments arranged by size:
3734 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2095 2176 2511 4655 2095
Size: 81 335 2144 2323
Fragments arranged by size:
2323 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1526 3250 1526
Size: 1724 3159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 882 1165 245
Size: 637 283 3963
Fragments arranged by size:
3963 637 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 2059 2938 3430 3728 1198
Size: 861 879 492 298 2353
Fragments arranged by size:
2353 879 861 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4587 4587
Size: 4883
BsaAI yAC'GTr
Cuts at: 555 632 2236 555
Size: 77 1604 3202
Fragments arranged by size:
3202 1604 77
BsaBI GATnn'nnATC
Cuts at: 6 2397 4434 6
Size: 2391 2037 455
Fragments arranged by size:
2391 2037 455
BsaHI Gr'CG_yC
Cuts at: 1433 2314 2562 3264 4760 1433
Size: 881 248 702 1496 1556
Fragments arranged by size:
1556 1496 881 702 248
BsaWI w'CCGG_w
Cuts at: 2072 2593 3271 3545 3692 4103 2072
Size: 521 678 274 147 411 2852
Fragments arranged by size:
2852 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 163 172 193 741 771 1141 1165 1171
Size: 21 9 21 548 30 370 24 6
Cuts at: 1171 1195 1423 1453 142
Size: 24 228 30 3572
Fragments arranged by size:
3572 548 370 228 30 30 24 24
21 21 9 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 246 1149 1183 1300 1303 1431 150
Size: 96 903 34 117 3 128 3602
Fragments arranged by size:
3602 903 128 117 96 34 3
BseYI C'CCAG_C
Cuts at: 1072 1293 2308 3066 3643 4139 1072
Size: 221 1015 758 577 496 1816
Fragments arranged by size:
1816 1015 758 577 496 221
BsiEI CG_ry'CG
Cuts at: 104 2425 2471 3679 104
Size: 2321 46 1208 1308
Fragments arranged by size:
2321 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1214 1459 1897 2675 2865 3657 1214
Size: 245 438 778 190 792 2440
Fragments arranged by size:
2440 792 778 438 245 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1180 1180
Size: 4883
Bsp1286I G_dGCh'C
Cuts at: 711 794 1214 1459 1897 2508 2601 2675 2865
Size: 83 420 245 438 611 93 74 190
Cuts at: 2865 2927 3657 711
Size: 62 730 1937
Fragments arranged by size:
1937 730 611 438 420 245 190 93
83 74 62
BspEI T'CCGG_A
Cuts at: 4103 4103
Size: 4883
BspHI T'CATG_A
Cuts at: 497 4059 497
Size: 3562 1321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1266 1280 1933 2147 2449 2830 1266
Size: 14 653 214 302 381 3319
Fragments arranged by size:
3319 653 381 302 214 14
BsrBI CCG'CTC
Cuts at: 50 627 3175 3229 4713 4878 50
Size: 577 2548 54 1484 165 55
Fragments arranged by size:
2548 1484 577 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 2795 570
Size: 2225 2658
BsrFI r'CCGG_y
Cuts at: 661 2881 3062 3271 661
Size: 2220 181 209 2273
Fragments arranged by size:
2273 2220 209 181
BssHII G'CGCG_C
Cuts at: 1857 1857
Size: 4883
BssSI C'ACGA_G
Cuts at: 618 3154 3512 618
Size: 2536 358 1989
Fragments arranged by size:
2536 1989 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2260 2260
Size: 4883
BstBI TT'CG_AA
Cuts at: 3244 3244
Size: 4883
BstEII G'GTnAC_C
Cuts at: 348 348
Size: 4883
BstYI r'GATC_y
Cuts at: 1 866 1908 2375 2398 2733 2979 3991 4077
Size: 865 1042 467 23 335 246 1012 86
Cuts at: 4077 4089 4435 1
Size: 12 346 449
Fragments arranged by size:
1042 1012 865 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 513 1283 513
Size: 770 4113
BtgI C'CryG_G
Cuts at: 956 1409 956
Size: 453 4430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 303 801 2833 3014 3177 303
Size: 498 2032 181 163 2009
Fragments arranged by size:
2032 2009 498 181 163
BtsI GCAGTG_nn'
Cuts at: 1113 2126 1113
Size: 1013 3870
ClaI AT'CG_AT
Cuts at: 504 504
Size: 4883
DraI TTT'AAA
Cuts at: 1882 1882
Size: 4883
DrdI GACnn_nn'nnGTC
Cuts at: 2589 3447 2589
Size: 858 4025
EaeI y'GGCC_r
Cuts at: 1032 1460 1496 2468 2642 3033 3060 4405 1032
Size: 428 36 972 174 391 27 1345 1510
Fragments arranged by size:
1510 1345 972 428 391 174 36 27
EagI C'GGCC_G
Cuts at: 2468 2468
Size: 4883
EarI CTCTTCn'nnn_
Cuts at: 1336 2906 3116 1336
Size: 1570 210 3103
Fragments arranged by size:
3103 1570 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1143 3091 3401 3547 4693 1143
Size: 1948 310 146 1146 1333
Fragments arranged by size:
1948 1333 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1895 1895
Size: 4883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 424 882 1165 1534 2708 3140 3887 4155
Size: 179 458 283 369 1174 432 747 268
Cuts at: 4155 245
Size: 973
Fragments arranged by size:
1174 973 747 458 432 369 283 268
179
EcoNI CCTnn'n_nnAGG
Cuts at: 287 1558 287
Size: 1271 3612
EcoO109I rG'GnC_Cy
Cuts at: 2022 2022
Size: 4883
EcoRI G'AATT_C
Cuts at: 1887 1887
Size: 4883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4883
FspI TGC'GCA
Cuts at: 2664 2664
Size: 4883
HaeII r_GCGC'y
Cuts at: 2317 2565 3587 4566 2317
Size: 248 1022 979 2634
Fragments arranged by size:
2634 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 660 692 1165 1197 1423 1455 1509 1541 1826
Size: 32 473 32 226 32 54 32 285
Cuts at: 1826 1858 2725 2755 2757 2787 660
Size: 32 867 30 2 30 2756
Fragments arranged by size:
2756 867 473 285 226 54 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 393 414 1437 1922 393
Size: 21 1023 485 3354
Fragments arranged by size:
3354 1023 485 21
HindIII A'AGCT_T
Cuts at: 1216 1374 1384 1216
Size: 158 10 4715
Fragments arranged by size:
4715 158 10
KasI G'GCGC_C
Cuts at: 2313 2561 2313
Size: 248 4635
KpnI G_GTAC'C
Cuts at: 272 1903 272
Size: 1631 3252
MluI A'CGCG_T
Cuts at: 4316 4316
Size: 4883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 720 1040 1096 1187 3554 3738 720
Size: 320 56 91 2367 184 1865
Fragments arranged by size:
2367 1865 320 184 91 56
MscI TGG'CCA
Cuts at: 1498 2644 1498
Size: 1146 3737
MslI CAynn'nnrTG
Cuts at: 469 496 844 2872 2999 469
Size: 27 348 2028 127 2353
Fragments arranged by size:
2353 2028 348 127 27
MspA1I CmG'CkG
Cuts at: 244 402 1037 1664 1994 2308 2668 3681 3926
Size: 158 635 627 330 314 360 1013 245
Cuts at: 3926 244
Size: 1201
Fragments arranged by size:
1201 1013 635 627 360 330 314 245
158
NaeI GCC'GGC
Cuts at: 3064 3064
Size: 4883
NarI GG'CG_CC
Cuts at: 2314 2562 2314
Size: 248 4635
NgoMIV G'CCGG_C
Cuts at: 3062 3062
Size: 4883
NspI r_CATG'y
Cuts at: 1936 2436 3343 1936
Size: 500 907 3476
Fragments arranged by size:
3476 907 500
PciI A'CATG_T
Cuts at: 3339 3339
Size: 4883
PflMI CCAn_nnn'nTGG
Cuts at: 1064 1064
Size: 4883
PfoI T'CCnGG_A
Cuts at: 163 759 163
Size: 596 4287
PmeI GTTT'AAAC
Cuts at: 1882 1882
Size: 4883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 227 741 773 1141 1173 4022 4054 195
Size: 32 514 32 368 32 2849 32 1024
Fragments arranged by size:
2849 1024 514 368 32 32 32 32
PstI C_TGCA'G
Cuts at: 979 1277 1334 1930 2615 979
Size: 298 57 596 685 3247
Fragments arranged by size:
3247 685 596 298 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4883
PvuII CAG'CTG
Cuts at: 244 402 1664 2308 2668 244
Size: 158 1262 644 360 2459
Fragments arranged by size:
2459 1262 644 360 158
RsrII CG'GwC_CG
Cuts at: 3078 3078
Size: 4883
SacI G_AGCT'C
Cuts at: 1897 1897
Size: 4883
SalI G'TCGA_C
Cuts at: 1920 1920
Size: 4883
SapI GCTCTTCn'nnn_
Cuts at: 1336 2906 3116 1336
Size: 1570 210 3103
Fragments arranged by size:
3103 1570 210
SbfI CC_TGCA'GG
Cuts at: 1277 1930 1277
Size: 653 4230
ScaI AGT'ACT
Cuts at: 4400 4400
Size: 4883
SfcI C'TryA_G
Cuts at: 33 975 1273 1330 1926 2611 3604 3795 33
Size: 942 298 57 596 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 596 298 191 57
SfoI GGC'GCC
Cuts at: 2315 2563 2315
Size: 248 4635
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
SmaI CCC'GGG
Cuts at: 200 1905 200
Size: 1705 3178
SmlI C'TyrA_G
Cuts at: 236 429 1213 2038 2953 3445 3707 236
Size: 193 784 825 915 492 262 1412
Fragments arranged by size:
1412 915 825 784 492 262 193
SnaBI TAC'GTA
Cuts at: 555 555
Size: 4883
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4883
SphI G_CATG'C
Cuts at: 1936 2436 1936
Size: 500 4383
StuI AGG'CCT
Cuts at: 1873 1873
Size: 4883
StyI C'CwwG_G
Cuts at: 344 1874 2017 2371 344
Size: 1530 143 354 2856
Fragments arranged by size:
2856 1530 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 664 1830 2466 2599 3058 3267 664
Size: 1166 636 133 459 209 2280
Fragments arranged by size:
2280 1166 636 459 209 133
TatI w'GTAC_w
Cuts at: 127 4398 127
Size: 4271 612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 4400 4432 163
Size: 32 4205 32 614
Fragments arranged by size:
4205 614 32 32
Tth111I GACn'n_nGTC
Cuts at: 2680 2680
Size: 4883
XbaI T'CTAG_A
Cuts at: 1914 1914
Size: 4883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2378 2378
Size: 4883
XhoI C'TCGA_G
Cuts at: 236 236
Size: 4883
XmaI C'CCGG_G
Cuts at: 198 1903 198
Size: 1705 3178
ZraI GAC'GTC
Cuts at: 1434 1434
Size: 4883
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AleI AlfI ApaI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsrGI
BstXI BstZ17I CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI
XmnI