Restriction map for
pF1KB3767
(Circular) (Six-base) MAPSORT of: pF1KB3767.seq Check: 2717 from: 1 to: 6392
pF1KB3767 6392 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 748 2039 3430 748
Size: 1291 1391 3710
Fragments arranged by size:
3710 1391 1291
Acc65I G'GTAC_C
Cuts at: 2702 3408 2702
Size: 706 5686
AclI AA'CG_TT
Cuts at: 6232 6232
Size: 6392
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 647 1247 1259 2017 2329 3182 4217 4649
Size: 266 600 12 758 312 853 1035 432
Cuts at: 4649 5396 5664 381
Size: 747 268 1109
Fragments arranged by size:
1109 1035 853 758 747 600 432 312
268 266 12
AflIII A'CryG_T
Cuts at: 3744 4848 5825 3744
Size: 1104 977 4311
Fragments arranged by size:
4311 1104 977
AgeI A'CCGG_T
Cuts at: 4780 4780
Size: 6392
AlwNI CAG_nnn'CTG
Cuts at: 156 1815 2385 3697 5264 156
Size: 1659 570 1312 1567 1284
Fragments arranged by size:
1659 1567 1312 1284 570
ApaLI G'TGCA_C
Cuts at: 5162 5162
Size: 6392
ApoI r'AATT_y
Cuts at: 390 1462 1992 2436 2582 2610 3396 5745 6001
Size: 1072 530 444 146 28 786 2349 256
Cuts at: 6001 390
Size: 781
Fragments arranged by size:
2349 1072 786 781 530 444 256 146
28
AseI AT'TA_AT
Cuts at: 20 20
Size: 6392
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6392
AvaI C'yCGr_G
Cuts at: 930 3412 930
Size: 2482 3910
BamHI G'GATC_C
Cuts at: 2455 3417 2455
Size: 962 5430
BanI G'GyrC_C
Cuts at: 672 2702 3408 3822 4070 4105 672
Size: 2030 706 414 248 35 2959
Fragments arranged by size:
2959 2030 706 414 248 35
BanII G_rGCy'C
Cuts at: 922 931 3406 4436 922
Size: 9 2475 1030 2878
Fragments arranged by size:
2878 2475 1030 9
BbeI G_GCGC'C
Cuts at: 3826 4074 3826
Size: 248 6144
BbsI GAAGACnn'nnnn_
Cuts at: 818 955 2023 2585 818
Size: 137 1068 562 4625
Fragments arranged by size:
4625 1068 562 137
BciVI GTATCCnnnnn_n'
Cuts at: 273 4290 5051 6392 273
Size: 4017 761 1341 273
Fragments arranged by size:
4017 1341 761 273
BclI T'GATC_A
Cuts at: 2979 3912 2979
Size: 933 5459
BglII A'GATC_T
Cuts at: 2019 3321 3907 5944 2019
Size: 1302 586 2037 2467
Fragments arranged by size:
2467 2037 1302 586
BlpI GC'TnA_GC
Cuts at: 146 921 1391 3504 146
Size: 775 470 2113 3034
Fragments arranged by size:
3034 2113 775 470
Bme1580I G_kGCm'C
Cuts at: 4017 4110 5166 4017
Size: 93 1056 5243
Fragments arranged by size:
5243 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3604 3685 4020 6164 3604
Size: 81 335 2144 3832
Fragments arranged by size:
3832 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4759 4759
Size: 6392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 446 2341 2704 120
Size: 326 1895 363 3808
Fragments arranged by size:
3808 1895 363 326
Bpu10I CC'TnA_GC
Cuts at: 3091 3091
Size: 6392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2828 2895 3568 4447 4939 5237 2828
Size: 67 673 879 492 298 3983
Fragments arranged by size:
3983 879 673 492 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 220 6096 220
Size: 5876 516
BsaAI yAC'GTr
Cuts at: 3745 3745
Size: 6392
BsaBI GATnn'nnATC
Cuts at: 6 3251 3906 5943 6
Size: 3245 655 2037 455
Fragments arranged by size:
3245 2037 655 455
BsaHI Gr'CG_yC
Cuts at: 3823 4071 4773 6269 3823
Size: 248 702 1496 3946
Fragments arranged by size:
3946 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3581 4102 4780 5054 5201 5612 3581
Size: 521 678 274 147 411 4361
Fragments arranged by size:
4361 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 819 849 1110 1140 1483 1513 819
Size: 30 261 30 343 30 5698
Fragments arranged by size:
5698 343 261 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 827 1927 220
Size: 607 1100 4685
Fragments arranged by size:
4685 1100 607
BseYI C'CCAG_C
Cuts at: 128 2648 3817 4575 5152 5648 128
Size: 2520 1169 758 577 496 872
Fragments arranged by size:
2520 1169 872 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 2573 1801
Size: 772 5620
BsiEI CG_ry'CG
Cuts at: 104 3934 3980 5188 104
Size: 3830 46 1208 1308
Fragments arranged by size:
3830 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 922 931 3406 4184 4374 5166 922
Size: 9 2475 778 190 792 2148
Fragments arranged by size:
2475 2148 792 778 190 9
BsmBI CGTCTCn'nnnn_
Cuts at: 2749 2749
Size: 6392
Bsp1286I G_dGCh'C
Cuts at: 922 931 3406 4017 4110 4184 4374 4436 5166
Size: 9 2475 611 93 74 190 62 730
Cuts at: 5166 922
Size: 2148
Fragments arranged by size:
2475 2148 730 611 190 93 74 62
9
BspEI T'CCGG_A
Cuts at: 5612 5612
Size: 6392
BspHI T'CATG_A
Cuts at: 897 2976 5568 897
Size: 2079 2592 1721
Fragments arranged by size:
2592 2079 1721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 595 1413 3442 3656 3958 4339 595
Size: 818 2029 214 302 381 2648
Fragments arranged by size:
2648 2029 818 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4684 4738 6222 6387 50
Size: 4634 54 1484 165 55
Fragments arranged by size:
4634 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2278 2416 4304 2278
Size: 138 1888 4366
Fragments arranged by size:
4366 1888 138
BsrFI r'CCGG_y
Cuts at: 4390 4571 4780 4390
Size: 181 209 6002
Fragments arranged by size:
6002 209 181
BsrGI T'GTAC_A
Cuts at: 1098 3344 1098
Size: 2246 4146
BssSI C'ACGA_G
Cuts at: 4663 5021 4663
Size: 358 6034
BstAPI GCAn_nnn'nTGC
Cuts at: 3769 3769
Size: 6392
BstBI TT'CG_AA
Cuts at: 4753 4753
Size: 6392
BstXI CCAn_nnnn'nTGG
Cuts at: 2259 2259
Size: 6392
BstYI r'GATC_y
Cuts at: 1 1769 2019 2455 3321 3417 3884 3907 4242
Size: 1768 250 436 866 96 467 23 335
Cuts at: 4242 4488 5500 5586 5598 5944 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1768 1012 866 467 449 436 346 335
250 246 96 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 794 2269 794
Size: 1475 4917
BtgI C'CryG_G
Cuts at: 1997 2597 1997
Size: 600 5792
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4342 4523 4686 4342
Size: 181 163 6048
Fragments arranged by size:
6048 181 163
BtsI GCAGTG_nn'
Cuts at: 1402 3635 1402
Size: 2233 4159
DraI TTT'AAA
Cuts at: 3391 3391
Size: 6392
DrdI GACnn_nn'nnGTC
Cuts at: 4098 4956 4098
Size: 858 5534
EaeI y'GGCC_r
Cuts at: 525 2025 3977 4151 4542 4569 5914 525
Size: 1500 1952 174 391 27 1345 1003
Fragments arranged by size:
1952 1500 1345 1003 391 174 27
EagI C'GGCC_G
Cuts at: 3977 3977
Size: 6392
EarI CTCTTCn'nnn_
Cuts at: 197 433 576 1904 1946 2747 3197 3293 4415
Size: 236 143 1328 42 801 450 96 1122
Cuts at: 4415 4625 197
Size: 210 1964
Fragments arranged by size:
1964 1328 1122 801 450 236 210 143
96 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4600 4910 5056 6202 4600
Size: 310 146 1146 4790
Fragments arranged by size:
4790 1146 310 146
EcoICRI GAG'CTC
Cuts at: 920 929 3404 920
Size: 9 2475 3908
Fragments arranged by size:
3908 2475 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 381 446 647 1247 1259 2017 2329 2341
Size: 261 65 201 600 12 758 312 12
Cuts at: 2341 2704 3182 4217 4649 5396 5664 120
Size: 363 478 1035 432 747 268 848
Fragments arranged by size:
1035 848 758 747 600 478 432 363
312 268 261 201 65 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 160 160
Size: 6392
EcoO109I rG'GnC_Cy
Cuts at: 153 1935 2265 3531 153
Size: 1782 330 1266 3014
Fragments arranged by size:
3014 1782 1266 330
EcoRI G'AATT_C
Cuts at: 2610 3396 2610
Size: 786 5606
EcoRV GAT'ATC
Cuts at: 1335 1335
Size: 6392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1726 2209 2587 4411 1726
Size: 483 378 1824 3707
Fragments arranged by size:
3707 1824 483 378
FspI TGC'GCA
Cuts at: 4173 4173
Size: 6392
HaeII r_GCGC'y
Cuts at: 3826 4074 5096 6075 3826
Size: 248 1022 979 4143
Fragments arranged by size:
4143 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 1135 1167 3308 3340 3365 3397 4234
Size: 32 163 32 2141 32 25 32 837
Cuts at: 4234 4264 4266 4296 940
Size: 30 2 30 3036
Fragments arranged by size:
3036 2141 837 163 32 32 32 32
30 30 25 2
HincII GTy'rAC
Cuts at: 1317 3431 1317
Size: 2114 4278
HindIII A'AGCT_T
Cuts at: 1538 1538
Size: 6392
HpaI GTT'AAC
Cuts at: 1317 1317
Size: 6392
KasI G'GCGC_C
Cuts at: 3822 4070 3822
Size: 248 6144
KpnI G_GTAC'C
Cuts at: 2706 3412 2706
Size: 706 5686
MluI A'CGCG_T
Cuts at: 5825 5825
Size: 6392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5063 5247 5063
Size: 184 6208
MscI TGG'CCA
Cuts at: 527 4153 527
Size: 3626 2766
MslI CAynn'nnrTG
Cuts at: 902 2257 2469 4381 4508 902
Size: 1355 212 1912 127 2786
Fragments arranged by size:
2786 1912 1355 212 127
MspA1I CmG'CkG
Cuts at: 1815 3503 3817 4177 5190 5435 1815
Size: 1688 314 360 1013 245 2772
Fragments arranged by size:
2772 1688 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4573 4573
Size: 6392
NarI GG'CG_CC
Cuts at: 3823 4071 3823
Size: 248 6144
NcoI C'CATG_G
Cuts at: 1997 2597 1997
Size: 600 5792
NgoMIV G'CCGG_C
Cuts at: 4571 4571
Size: 6392
NspI r_CATG'y
Cuts at: 3445 3945 4852 3445
Size: 500 907 4985
Fragments arranged by size:
4985 907 500
PasI CC'CwG_GG
Cuts at: 2451 2451
Size: 6392
PciI A'CATG_T
Cuts at: 4848 4848
Size: 6392
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 6392
PfoI T'CCnGG_A
Cuts at: 197 2357 197
Size: 2160 4232
PmeI GTTT'AAAC
Cuts at: 3391 3391
Size: 6392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 1076 5531 5563 1044
Size: 32 4455 32 1873
Fragments arranged by size:
4455 1873 32 32
PpuMI rG'GwC_Cy
Cuts at: 1935 2265 1935
Size: 330 6062
PshAI GACnn'nnGTC
Cuts at: 373 373
Size: 6392
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2686 2718 2686
Size: 32 6360
PstI C_TGCA'G
Cuts at: 1743 2202 3278 3439 4124 1743
Size: 459 1076 161 685 4011
Fragments arranged by size:
4011 1076 685 459 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6392
PvuII CAG'CTG
Cuts at: 1815 3817 4177 1815
Size: 2002 360 4030
Fragments arranged by size:
4030 2002 360
RsrII CG'GwC_CG
Cuts at: 4587 4587
Size: 6392
SacI G_AGCT'C
Cuts at: 922 931 3406 922
Size: 9 2475 3908
Fragments arranged by size:
3908 2475 9
SalI G'TCGA_C
Cuts at: 3429 3429
Size: 6392
SapI GCTCTTCn'nnn_
Cuts at: 4415 4625 4415
Size: 210 6182
SbfI CC_TGCA'GG
Cuts at: 3439 3439
Size: 6392
ScaI AGT'ACT
Cuts at: 5909 5909
Size: 6392
SexAI A'CCwGG_T
Cuts at: 2705 2705
Size: 6392
SfcI C'TryA_G
Cuts at: 33 944 1739 2198 2558 2944 3073 3274 3435
Size: 911 795 459 360 386 129 201 161
Cuts at: 3435 4120 5113 5304 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 911 795 685 459 386 360
201 191 161 129
SfoI GGC'GCC
Cuts at: 3824 4072 3824
Size: 248 6144
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6392
SmaI CCC'GGG
Cuts at: 3414 3414
Size: 6392
SmlI C'TyrA_G
Cuts at: 930 2843 2910 3547 4462 4954 5216 930
Size: 1913 67 637 915 492 262 2106
Fragments arranged by size:
2106 1913 915 637 492 262 67
SpeI A'CTAG_T
Cuts at: 67 3168 67
Size: 3101 3291
SphI G_CATG'C
Cuts at: 3445 3945 3445
Size: 500 5892
SspI AAT'ATT
Cuts at: 1980 1980
Size: 6392
StyI C'CwwG_G
Cuts at: 1997 2597 3526 3880 1997
Size: 600 929 354 4509
Fragments arranged by size:
4509 929 600 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3975 4108 4567 4776 3975
Size: 133 459 209 5591
Fragments arranged by size:
5591 459 209 133
TatI w'GTAC_w
Cuts at: 1098 3344 5907 1098
Size: 2246 2563 1583
Fragments arranged by size:
2563 2246 1583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1110 1142 1358 1390 5909 5941 1110
Size: 32 216 32 4519 32 1561
Fragments arranged by size:
4519 1561 216 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2466 3377 4189 2466
Size: 911 812 4669
Fragments arranged by size:
4669 911 812
XbaI T'CTAG_A
Cuts at: 1530 3135 3423 1530
Size: 1605 288 4499
Fragments arranged by size:
4499 1605 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3887 3887
Size: 6392
XhoI C'TCGA_G
Cuts at: 930 930
Size: 6392
XmaI C'CCGG_G
Cuts at: 3412 3412
Size: 6392
XmnI GAAnn'nnTTC
Cuts at: 255 3311 255
Size: 3056 3336
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsiWI BsmI BssHII BstEII BstZ17I ClaI
CspCI DraIII FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI ZraI