Restriction map for
pF1KB3788
(Circular) (Six-base) MAPSORT of: pF1KB3788.seq Check: 5413 from: 1 to: 5255
pF1KB3788 5255 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1348 2293 1348
Size: 945 4310
Acc65I G'GTAC_C
Cuts at: 2271 2271
Size: 5255
AclI AA'CG_TT
Cuts at: 5095 5095
Size: 5255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 676 984 1839 3080 3512 3865 4527 461
Size: 215 308 855 1241 432 353 662 1189
Fragments arranged by size:
1241 1189 855 662 432 353 308 215
AflIII A'CryG_T
Cuts at: 2607 4407 4688 2607
Size: 1800 281 3174
Fragments arranged by size:
3174 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 180 2560 3998 180
Size: 2380 1438 1437
Fragments arranged by size:
2380 1438 1437
ApaLI G'TGCA_C
Cuts at: 4093 4093
Size: 5255
ApoI r'AATT_y
Cuts at: 322 467 534 643 846 931 1414 1512 1674
Size: 145 67 109 203 85 483 98 162
Cuts at: 1674 1912 1952 2167 2204 2259 4608 4864 322
Size: 238 40 215 37 55 2349 256 713
Fragments arranged by size:
2349 713 483 256 238 215 203 162
145 109 98 85 67 55 40 37
AseI AT'TA_AT
Cuts at: 20 556 20
Size: 536 4719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5255
AvaI C'yCGr_G
Cuts at: 1424 2275 1424
Size: 851 4404
BamHI G'GATC_C
Cuts at: 2280 2280
Size: 5255
BanI G'GyrC_C
Cuts at: 1691 2271 2685 2933 2968 1691
Size: 580 414 248 35 3978
Fragments arranged by size:
3978 580 414 248 35
BanII G_rGCy'C
Cuts at: 954 2269 3299 954
Size: 1315 1030 2910
Fragments arranged by size:
2910 1315 1030
BbeI G_GCGC'C
Cuts at: 2689 2937 2689
Size: 248 5007
BbsI GAAGACnn'nnnn_
Cuts at: 398 1992 398
Size: 1594 3661
BciVI GTATCCnnnnn_n'
Cuts at: 654 3153 4209 5255 654
Size: 2499 1056 1046 654
Fragments arranged by size:
2499 1056 1046 654
BclI T'GATC_A
Cuts at: 2775 2775
Size: 5255
BfrBI ATG'CAT
Cuts at: 737 856 737
Size: 119 5136
BglII A'GATC_T
Cuts at: 2770 4807 2770
Size: 2037 3218
BlpI GC'TnA_GC
Cuts at: 2367 2367
Size: 5255
Bme1580I G_kGCm'C
Cuts at: 1694 2880 2973 4097 1694
Size: 1186 93 1124 2852
Fragments arranged by size:
2852 1186 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2467 2548 2883 5027 2467
Size: 81 335 2144 2695
Fragments arranged by size:
2695 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3622 3622
Size: 5255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 372
Size: 5255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2431 3310 4024 4322 2431
Size: 879 714 298 3364
Fragments arranged by size:
3364 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4959 4959
Size: 5255
BsaAI yAC'GTr
Cuts at: 2608 2608
Size: 5255
BsaBI GATnn'nnATC
Cuts at: 6 2769 4806 6
Size: 2763 2037 455
Fragments arranged by size:
2763 2037 455
BsaHI Gr'CG_yC
Cuts at: 2686 2934 3636 5132 2686
Size: 248 702 1496 2809
Fragments arranged by size:
2809 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2444 2965 3643 4054 4201 4475 2444
Size: 521 678 411 147 274 3224
Fragments arranged by size:
3224 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 313 1060 181
Size: 132 747 4376
Fragments arranged by size:
4376 747 132
BseYI C'CCAG_C
Cuts at: 2680 3438 4103 4511 2680
Size: 758 665 408 3424
Fragments arranged by size:
3424 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 1177
Size: 5255
BsiEI CG_ry'CG
Cuts at: 104 2797 2843 4073 104
Size: 2693 46 1230 1286
Fragments arranged by size:
2693 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2269 3047 3237 4097 2269
Size: 778 190 860 3427
Fragments arranged by size:
3427 860 778 190
BsmI GAATG_Cn'
Cuts at: 739 898 739
Size: 159 5096
Bsp1286I G_dGCh'C
Cuts at: 954 1694 2269 2880 2973 3047 3237 3299 4097
Size: 740 575 611 93 74 190 62 798
Cuts at: 4097 954
Size: 2112
Fragments arranged by size:
2112 798 740 611 575 190 93 74
62
BspHI T'CATG_A
Cuts at: 3687 3687
Size: 5255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2305 2519 2821 3202 2305
Size: 214 302 381 4358
Fragments arranged by size:
4358 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3547 3601 5085 5250 50
Size: 3497 54 1484 165 55
Fragments arranged by size:
3497 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 115 3167 115
Size: 3052 2203
BsrFI r'CCGG_y
Cuts at: 3253 3434 3253
Size: 181 5074
BsrGI T'GTAC_A
Cuts at: 1023 2109 1023
Size: 1086 4169
BssSI C'ACGA_G
Cuts at: 3526 4234 3526
Size: 708 4547
BstAPI GCAn_nnn'nTGC
Cuts at: 2632 2632
Size: 5255
BstBI TT'CG_AA
Cuts at: 3616 3616
Size: 5255
BstXI CCAn_nnnn'nTGG
Cuts at: 350 350
Size: 5255
BstYI r'GATC_y
Cuts at: 1 2280 2747 2770 3105 3351 3657 3669 3755
Size: 2279 467 23 335 246 306 12 86
Cuts at: 3755 4807 1
Size: 1052 449
Fragments arranged by size:
2279 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 304 304
Size: 5255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3205 3386 3549 3205
Size: 181 163 4911
Fragments arranged by size:
4911 181 163
BtsI GCAGTG_nn'
Cuts at: 903 2498 903
Size: 1595 3660
DraI TTT'AAA
Cuts at: 1511 2254 1511
Size: 743 4512
DrdI GACnn_nn'nnGTC
Cuts at: 2961 4305 2961
Size: 1344 3911
EaeI y'GGCC_r
Cuts at: 2840 3014 3405 3432 4777 2840
Size: 174 391 27 1345 3318
Fragments arranged by size:
3318 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2840 2840
Size: 5255
EarI CTCTTCn'nnn_
Cuts at: 437 1460 1544 1622 1790 1858 2208 3278 3488
Size: 1023 84 78 168 68 350 1070 210
Cuts at: 3488 437
Size: 2204
Fragments arranged by size:
2204 1070 1023 350 210 168 84 78
68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3463 4205 4351 5065 3463
Size: 742 146 714 3653
Fragments arranged by size:
3653 742 714 146
EcoICRI GAG'CTC
Cuts at: 2267 2267
Size: 5255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 461 676 984 1839 3080 3512 3865 4527
Size: 89 215 308 855 1241 432 353 662
Cuts at: 4527 372
Size: 1100
Fragments arranged by size:
1241 1100 855 662 432 353 308 215
89
EcoO109I rG'GnC_Cy
Cuts at: 1220 2394 1220
Size: 1174 4081
EcoRI G'AATT_C
Cuts at: 1952 2204 2259 1952
Size: 252 55 4948
Fragments arranged by size:
4948 252 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3274 3274
Size: 5255
FspI TGC'GCA
Cuts at: 3036 3036
Size: 5255
HaeII r_GCGC'y
Cuts at: 2689 2937 4167 4938 2689
Size: 248 1230 771 3006
Fragments arranged by size:
3006 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1209 1241 1347 1379 3097 3127 3129 3159 1209
Size: 32 106 32 1718 30 2 30 3305
Fragments arranged by size:
3305 1718 106 32 32 30 30 2
HincII GTy'rAC
Cuts at: 334 2294 334
Size: 1960 3295
HindIII A'AGCT_T
Cuts at: 1303 1303
Size: 5255
KasI G'GCGC_C
Cuts at: 2685 2933 2685
Size: 248 5007
KpnI G_GTAC'C
Cuts at: 2275 2275
Size: 5255
MluI A'CGCG_T
Cuts at: 4688 4688
Size: 5255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 266 4014 4198 200
Size: 66 3748 184 1257
Fragments arranged by size:
3748 1257 184 66
MscI TGG'CCA
Cuts at: 3016 3016
Size: 5255
MslI CAynn'nnrTG
Cuts at: 515 838 3244 3371 515
Size: 323 2406 127 2399
Fragments arranged by size:
2406 2399 323 127
MspA1I CmG'CkG
Cuts at: 180 2366 2680 3040 3824 4069 180
Size: 2186 314 360 784 245 1366
Fragments arranged by size:
2186 1366 784 360 314 245
NaeI GCC'GGC
Cuts at: 3436 3436
Size: 5255
NarI GG'CG_CC
Cuts at: 2686 2934 2686
Size: 248 5007
NgoMIV G'CCGG_C
Cuts at: 3434 3434
Size: 5255
NsiI A_TGCA'T
Cuts at: 739 858 739
Size: 119 5136
NspI r_CATG'y
Cuts at: 2308 2808 4411 2308
Size: 500 1603 3152
Fragments arranged by size:
3152 1603 500
PciI A'CATG_T
Cuts at: 4407 4407
Size: 5255
PmeI GTTT'AAAC
Cuts at: 2254 2254
Size: 5255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 5223
PsiI TTA'TAA
Cuts at: 1310 1310
Size: 5255
PstI C_TGCA'G
Cuts at: 2302 2987 2302
Size: 685 4570
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5255
PvuII CAG'CTG
Cuts at: 180 2680 3040 180
Size: 2500 360 2395
Fragments arranged by size:
2500 2395 360
RsrII CG'GwC_CG
Cuts at: 3450 3450
Size: 5255
SacI G_AGCT'C
Cuts at: 2269 2269
Size: 5255
SalI G'TCGA_C
Cuts at: 2292 2292
Size: 5255
SapI GCTCTTCn'nnn_
Cuts at: 3278 3488 3278
Size: 210 5045
SbfI CC_TGCA'GG
Cuts at: 2302 2302
Size: 5255
ScaI AGT'ACT
Cuts at: 4772 4772
Size: 5255
SfcI C'TryA_G
Cuts at: 33 228 2031 2115 2298 2983 3951 4142 33
Size: 195 1803 84 183 685 968 191 1146
Fragments arranged by size:
1803 1146 968 685 195 191 183 84
SfoI GGC'GCC
Cuts at: 2687 2935 2687
Size: 248 5007
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5255
SmaI CCC'GGG
Cuts at: 2277 2277
Size: 5255
SmlI C'TyrA_G
Cuts at: 2410 3325 4039 4301 2410
Size: 915 714 262 3364
Fragments arranged by size:
3364 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5255
SphI G_CATG'C
Cuts at: 2308 2808 2308
Size: 500 4755
SspI AAT'ATT
Cuts at: 687 687
Size: 5255
StyI C'CwwG_G
Cuts at: 2389 2743 2389
Size: 354 4901
SwaI ATTT'AAAT
Cuts at: 1511 1511
Size: 5255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 2838 2971 3430 3639 175
Size: 2663 133 459 209 1791
Fragments arranged by size:
2663 1791 459 209 133
TatI w'GTAC_w
Cuts at: 1023 1539 1869 2109 2229 4770 1023
Size: 516 330 240 120 2541 1508
Fragments arranged by size:
2541 1508 516 330 240 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4772 4804 4772
Size: 32 5223
Tth111I GACn'n_nGTC
Cuts at: 3052 3052
Size: 5255
XbaI T'CTAG_A
Cuts at: 2286 2286
Size: 5255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2750 2750
Size: 5255
XmaI C'CCGG_G
Cuts at: 2275 2275
Size: 5255
XmnI GAAnn'nnTTC
Cuts at: 686 997 1122 1165 1249 1663 1788 1956 2040
Size: 311 125 43 84 414 125 168 84
Cuts at: 2040 2124 2167 686
Size: 84 43 3774
Fragments arranged by size:
3774 414 311 168 125 125 84 84
84 43 43
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BspEI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI XhoI ZraI