Restriction map for
pF1KB3789
(Circular) (Six-base) MAPSORT of: pF1KB3789.seq Check: 9346 from: 1 to: 7706
pF1KB3789 7706 bp
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2598 2598
Size: 7706
AatII G_ACGT'C
Cuts at: 2618 4312 2618
Size: 1694 6012
AccI GT'mk_AC
Cuts at: 638 1808 4744 638
Size: 1170 2936 3600
Fragments arranged by size:
3600 2936 1170
Acc65I G'GTAC_C
Cuts at: 4722 4722
Size: 7706
AclI AA'CG_TT
Cuts at: 7546 7546
Size: 7706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 1735 1744 2578 3547 3667 4351 5531 5963
Size: 1241 9 834 969 120 684 1180 432
Cuts at: 5963 6710 6978 494
Size: 747 268 1222
Fragments arranged by size:
1241 1222 1180 969 834 747 684 432
268 120 9
AfeI AGC'GCT
Cuts at: 744 3625 744
Size: 2881 4825
AflIII A'CryG_T
Cuts at: 851 5058 6162 7139 851
Size: 4207 1104 977 1418
Fragments arranged by size:
4207 1418 1104 977
AgeI A'CCGG_T
Cuts at: 6094 6094
Size: 7706
AhdI GACnn_n'nnGTC
Cuts at: 831 2008 4341 831
Size: 1177 2333 4196
Fragments arranged by size:
4196 2333 1177
AleI CACnn'nnGTG
Cuts at: 3638 4594 3638
Size: 956 6750
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 472 1942 472
Size: 1470 6236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3319 3351 3319
Size: 32 7674
AlwNI CAG_nnn'CTG
Cuts at: 813 2670 3101 3916 3965 5011 6578 813
Size: 1857 431 815 49 1046 1567 1941
Fragments arranged by size:
1941 1857 1567 1046 815 431 49
ApaI G_GGCC'C
Cuts at: 1076 1498 4437 1076
Size: 422 2939 4345
Fragments arranged by size:
4345 2939 422
ApaLI G'TGCA_C
Cuts at: 1794 6476 1794
Size: 4682 3024
ApoI r'AATT_y
Cuts at: 1628 3988 4710 7059 7315 1628
Size: 2360 722 2349 256 2019
Fragments arranged by size:
2360 2349 2019 722 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7706
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7706
AvaI C'yCGr_G
Cuts at: 2444 2678 3535 4430 4726 2444
Size: 234 857 895 296 5424
Fragments arranged by size:
5424 895 857 296 234
BamHI G'GATC_C
Cuts at: 948 4731 948
Size: 3783 3923
BanI G'GyrC_C
Cuts at: 228 1536 1753 2686 2885 3415 3927 4633 4722
Size: 1308 217 933 199 530 512 706 89
Cuts at: 4722 5136 5384 5419 228
Size: 414 248 35 2515
Fragments arranged by size:
2515 1308 933 706 530 512 414 248
217 199 89 35
BanII G_rGCy'C
Cuts at: 1076 1498 2227 2659 3171 3397 4437 4720 5750
Size: 422 729 432 512 226 1040 283 1030
Cuts at: 5750 1076
Size: 3032
Fragments arranged by size:
3032 1040 1030 729 512 432 422 283
226
BbeI G_GCGC'C
Cuts at: 2690 3931 4637 5140 5388 2690
Size: 1241 706 503 248 5008
Fragments arranged by size:
5008 1241 706 503 248
BbsI GAAGACnn'nnnn_
Cuts at: 500 1570 4453 500
Size: 1070 2883 3753
Fragments arranged by size:
3753 2883 1070
BbvCI CC'TCA_GC
Cuts at: 2918 3037 4441 2918
Size: 119 1404 6183
Fragments arranged by size:
6183 1404 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3717 3751 4161 4195 3717
Size: 34 410 34 7228
Fragments arranged by size:
7228 410 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5604 6365 7706 5604
Size: 761 1341 5604
Fragments arranged by size:
5604 1341 761
BclI T'GATC_A
Cuts at: 1334 5226 1334
Size: 3892 3814
BglI GCCn_nnn'nGGC
Cuts at: 2147 2182 2147
Size: 35 7671
BglII A'GATC_T
Cuts at: 1139 2642 5221 7258 1139
Size: 1503 2579 2037 1587
Fragments arranged by size:
2579 2037 1587 1503
BlpI GC'TnA_GC
Cuts at: 2848 3094 3154 4818 2848
Size: 246 60 1664 5736
Fragments arranged by size:
5736 1664 246 60
Bme1580I G_kGCm'C
Cuts at: 555 1076 1498 1541 1756 1798 2029 2210 2343
Size: 521 422 43 215 42 231 181 133
Cuts at: 2343 4437 5331 5424 6480 555
Size: 2094 894 93 1056 1781
Fragments arranged by size:
2094 1781 1056 894 521 422 231 215
181 133 93 43 42
BmgBI CAC'GTC
Cuts at: 854 854
Size: 7706
BmrI ACTGGGnnnn_n'
Cuts at: 412 2363 3006 3305 4918 4999 5334 7478 412
Size: 1951 643 299 1613 81 335 2144 640
Fragments arranged by size:
2144 1951 1613 643 640 335 299 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2096 2673 6073 2096
Size: 577 3400 3729
Fragments arranged by size:
3729 3400 577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 835 1532 2323 3736 3753 4336 505
Size: 330 697 791 1413 17 583 3875
Fragments arranged by size:
3875 1413 791 697 583 330 17
Bpu10I CC'TnA_GC
Cuts at: 2918 3037 4441 2918
Size: 119 1404 6183
Fragments arranged by size:
6183 1404 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4882 5761 6253 6551 3883
Size: 999 879 492 298 5038
Fragments arranged by size:
5038 999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2929 3571 7410 2929
Size: 642 3839 3225
Fragments arranged by size:
3839 3225 642
BsaAI yAC'GTr
Cuts at: 170 1424 1812 2211 3255 3507 3833 5059 170
Size: 1254 388 399 1044 252 326 1226 2817
Fragments arranged by size:
2817 1254 1226 1044 399 388 326 252
BsaBI GATnn'nnATC
Cuts at: 6 4162 4219 5220 7257 6
Size: 4156 57 1001 2037 455
Fragments arranged by size:
4156 2037 1001 455 57
BsaHI Gr'CG_yC
Cuts at: 2615 2687 2762 3928 4309 4634 4688 5137 5385
Size: 72 75 1166 381 325 54 449 248
Cuts at: 5385 6087 7583 2615
Size: 702 1496 2738
Fragments arranged by size:
2738 1496 1166 702 449 381 325 248
75 72 54
BsaWI w'CCGG_w
Cuts at: 1883 3992 4523 4895 5416 6094 6368 6515 6926
Size: 2109 531 372 521 678 274 147 411
Cuts at: 6926 1883
Size: 2663
Fragments arranged by size:
2663 2109 678 531 521 411 372 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 1505 1535 3319 3349 4058 4088 838
Size: 30 637 30 1784 30 709 30 4456
Fragments arranged by size:
4456 1784 709 637 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 504 930 1006 1723 1990 2335 2758 2847 2850
Size: 426 76 717 267 345 423 89 3
Cuts at: 2850 2947 3628 504
Size: 97 681 4582
Fragments arranged by size:
4582 717 681 426 423 345 267 97
89 76 3
BseYI C'CCAG_C
Cuts at: 362 956 3585 3679 3958 4099 5131 5889 6466
Size: 594 2629 94 279 141 1032 758 577
Cuts at: 6466 6962 362
Size: 496 1106
Fragments arranged by size:
2629 1106 1032 758 594 577 496 279
141 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 1755 1948 2843 3235 3250 4180 4397 4527
Size: 521 193 895 392 15 930 217 130
Cuts at: 4527 1234
Size: 4413
Fragments arranged by size:
4413 930 895 521 392 217 193 130
15
BsiEI CG_ry'CG
Cuts at: 104 1367 5248 5294 6502 104
Size: 1263 3881 46 1208 1308
Fragments arranged by size:
3881 1308 1263 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1798 3140 4720 5498 5688 6480 1798
Size: 1342 1580 778 190 792 3024
Fragments arranged by size:
3024 1580 1342 792 778 190
BsmI GAATG_Cn'
Cuts at: 904 1675 2055 904
Size: 771 380 6555
Fragments arranged by size:
6555 771 380
BsmBI CGTCTCn'nnnn_
Cuts at: 2675 2675
Size: 7706
Bsp1286I G_dGCh'C
Cuts at: 555 1076 1498 1541 1756 1798 2029 2210 2227
Size: 521 422 43 215 42 231 181 17
Cuts at: 2227 2343 2659 3140 3171 3397 4437 4720 5331
Size: 116 316 481 31 226 1040 283 611
Cuts at: 5331 5424 5498 5688 5750 6480 555
Size: 93 74 190 62 730 1781
Fragments arranged by size:
1781 1040 730 611 521 481 422 316
283 231 226 215 190 181 116 93
74 62 43 42 31 17
BspEI T'CCGG_A
Cuts at: 3992 4523 6926 3992
Size: 531 2403 4772
Fragments arranged by size:
4772 2403 531
BspHI T'CATG_A
Cuts at: 1085 2627 6882 1085
Size: 1542 4255 1909
Fragments arranged by size:
4255 1909 1542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2598 2967 3269 3813 4756 4970 5272 5653 2598
Size: 369 302 544 943 214 302 381 4651
Fragments arranged by size:
4651 943 544 381 369 302 302 214
BsrBI CCG'CTC
Cuts at: 50 150 3303 3551 5998 6052 7536 7701 50
Size: 100 3153 248 2447 54 1484 165 55
Fragments arranged by size:
3153 2447 1484 248 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 3817 5618 3817
Size: 1801 5905
BsrFI r'CCGG_y
Cuts at: 921 4565 4690 5704 5885 6094 921
Size: 3644 125 1014 181 209 2533
Fragments arranged by size:
3644 2533 1014 209 181 125
BssSI C'ACGA_G
Cuts at: 220 1243 5977 6335 220
Size: 1023 4734 358 1591
Fragments arranged by size:
4734 1591 1023 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2503 5083 2503
Size: 2580 5126
BstBI TT'CG_AA
Cuts at: 6067 6067
Size: 7706
BstEII G'GTnAC_C
Cuts at: 3281 3825 3281
Size: 544 7162
BstXI CCAn_nnnn'nTGG
Cuts at: 3345 3687 3882 3345
Size: 342 195 7169
Fragments arranged by size:
7169 342 195
BstYI r'GATC_y
Cuts at: 1 568 948 1139 1958 2573 2642 3230 4454
Size: 567 380 191 819 615 69 588 1224
Cuts at: 4454 4731 5198 5221 5556 5802 6814 6900 6912
Size: 277 467 23 335 246 1012 86 12
Cuts at: 6912 7258 1
Size: 346 449
Fragments arranged by size:
1224 1012 819 615 588 567 467 449
380 346 335 277 246 191 86 69
23 12
Bsu36I CC'TnA_GG
Cuts at: 1954 1954
Size: 7706
BtgI C'CryG_G
Cuts at: 107 1361 1748 1907 2132 2228 3209 3887 4475
Size: 1254 387 159 225 96 981 678 588
Cuts at: 4475 4484 107
Size: 9 3329
Fragments arranged by size:
3329 1254 981 678 588 387 225 159
96 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 3432 4164 4542 5656 5837 6000 1573
Size: 1859 732 378 1114 181 163 3279
Fragments arranged by size:
3279 1859 1114 732 378 181 163
BtsI GCAGTG_nn'
Cuts at: 828 1223 2504 4949 828
Size: 395 1281 2445 3585
Fragments arranged by size:
3585 2445 1281 395
ClaI AT'CG_AT
Cuts at: 4166 4166
Size: 7706
DraI TTT'AAA
Cuts at: 4705 4705
Size: 7706
DraIII CAC_nnn'GTG
Cuts at: 200 200
Size: 7706
DrdI GACnn_nn'nnGTC
Cuts at: 163 3118 4546 5412 6270 163
Size: 2955 1428 866 858 1599
Fragments arranged by size:
2955 1599 1428 866 858
EaeI y'GGCC_r
Cuts at: 1418 1694 2129 3212 3437 3689 3704 3884 4679
Size: 276 435 1083 225 252 15 180 795
Cuts at: 4679 5291 5465 5856 5883 7228 1418
Size: 612 174 391 27 1345 1896
Fragments arranged by size:
1896 1345 1083 795 612 435 391 276
252 225 180 174 27 15
EagI C'GGCC_G
Cuts at: 5291 5291
Size: 7706
EarI CTCTTCn'nnn_
Cuts at: 379 1588 2482 3028 5729 5939 379
Size: 1209 894 546 2701 210 2146
Fragments arranged by size:
2701 2146 1209 894 546 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3944 4104 5914 6224 6370 7516 3944
Size: 160 1810 310 146 1146 4134
Fragments arranged by size:
4134 1810 1146 310 160 146
EcoICRI GAG'CTC
Cuts at: 4718 4718
Size: 7706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 505 835 1532 1735 1744 2323 2578 3547
Size: 11 330 697 203 9 579 255 969
Cuts at: 3547 3667 3736 3753 4336 4351 5531 5963 6710
Size: 120 69 17 583 15 1180 432 747
Cuts at: 6710 6978 494
Size: 268 1222
Fragments arranged by size:
1222 1180 969 747 697 583 579 432
330 268 255 203 120 69 17 15
11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2067 2067
Size: 7706
EcoO109I rG'GnC_Cy
Cuts at: 1073 1179 1326 1495 4020 4233 4434 4845 1073
Size: 106 147 169 2525 213 201 411 3934
Fragments arranged by size:
3934 2525 411 213 201 169 147 106
EcoRI G'AATT_C
Cuts at: 3988 4710 3988
Size: 722 6984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1480 5725 1480
Size: 4245 3461
FspI TGC'GCA
Cuts at: 5487 5487
Size: 7706
HaeII r_GCGC'y
Cuts at: 115 134 746 2690 3534 3627 3931 4413 4637
Size: 19 612 1944 844 93 304 482 224
Cuts at: 4637 5140 5388 6410 7389 115
Size: 503 248 1022 979 432
Fragments arranged by size:
1944 1022 979 844 612 503 482 432
304 248 224 93 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 297 329 817 838 849 870 1994 2026 2267
Size: 32 488 21 11 21 1124 32 241
Cuts at: 2267 2299 2476 2508 3011 3043 4058 4090 4145
Size: 32 177 32 503 32 1015 32 55
Cuts at: 4145 4177 4327 4359 5548 5578 5580 5610 297
Size: 32 150 32 1189 30 2 30 2393
Fragments arranged by size:
2393 1189 1124 1015 503 488 241 177
150 55 32 32 32 32 32 32
32 32 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 1380 3123 4745 1380
Size: 1743 1622 4341
Fragments arranged by size:
4341 1743 1622
HindIII A'AGCT_T
Cuts at: 1654 2983 1654
Size: 1329 6377
KasI G'GCGC_C
Cuts at: 2686 3927 4633 5136 5384 2686
Size: 1241 706 503 248 5008
Fragments arranged by size:
5008 1241 706 503 248
KpnI G_GTAC'C
Cuts at: 4726 4726
Size: 7706
MluI A'CGCG_T
Cuts at: 7139 7139
Size: 7706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 836 2483 3107 4445 6377 6561 167
Size: 669 1647 624 1338 1932 184 1312
Fragments arranged by size:
1932 1647 1338 1312 669 624 184
MscI TGG'CCA
Cuts at: 1420 1696 3439 3691 4681 5467 1420
Size: 276 1743 252 990 786 3659
Fragments arranged by size:
3659 1743 990 786 276 252
MslI CAynn'nnrTG
Cuts at: 304 454 1390 1432 2041 2626 2809 3214 3382
Size: 150 936 42 609 585 183 405 168
Cuts at: 3382 3638 4046 4373 4579 4594 5695 5822 304
Size: 256 408 327 206 15 1101 127 2188
Fragments arranged by size:
2188 1101 936 609 585 408 405 327
256 206 183 168 150 127 42 15
MspA1I CmG'CkG
Cuts at: 483 1300 2301 2768 3245 3525 3965 4817 5131
Size: 817 1001 467 477 280 440 852 314
Cuts at: 5131 5491 6504 6749 483
Size: 360 1013 245 1440
Fragments arranged by size:
1440 1013 1001 852 817 477 467 440
360 314 280 245
NaeI GCC'GGC
Cuts at: 4692 5887 4692
Size: 1195 6511
NarI GG'CG_CC
Cuts at: 2687 3928 4634 5137 5385 2687
Size: 1241 706 503 248 5008
Fragments arranged by size:
5008 1241 706 503 248
NcoI C'CATG_G
Cuts at: 107 1907 3209 107
Size: 1800 1302 4604
Fragments arranged by size:
4604 1800 1302
NgoMIV G'CCGG_C
Cuts at: 4690 5885 4690
Size: 1195 6511
NspI r_CATG'y
Cuts at: 2808 4759 5259 6166 2808
Size: 1951 500 907 4348
Fragments arranged by size:
4348 1951 907 500
PasI CC'CwG_GG
Cuts at: 3171 4119 3171
Size: 948 6758
PciI A'CATG_T
Cuts at: 6162 6162
Size: 7706
PflMI CCAn_nnn'nTGG
Cuts at: 813 3089 3698 3965 813
Size: 2276 609 267 4554
Fragments arranged by size:
4554 2276 609 267
PfoI T'CCnGG_A
Cuts at: 1835 3368 3941 4622 1835
Size: 1533 573 681 4919
Fragments arranged by size:
4919 1533 681 573
PmeI GTTT'AAAC
Cuts at: 4705 4705
Size: 7706
PmlI CAC'GTG
Cuts at: 170 2211 3255 170
Size: 2041 1044 4621
Fragments arranged by size:
4621 2041 1044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3319 3351 6845 6877 3319
Size: 32 3494 32 4148
Fragments arranged by size:
4148 3494 32 32
PpuMI rG'GwC_Cy
Cuts at: 4020 4233 4020
Size: 213 7493
PshAI GACnn'nnGTC
Cuts at: 1300 1300
Size: 7706
PspOMI G'GGCC_C
Cuts at: 1072 1494 4433 1072
Size: 422 2939 4345
Fragments arranged by size:
4345 2939 422
PspXI vC'TCGA_Gb
Cuts at: 3535 3535
Size: 7706
PstI C_TGCA'G
Cuts at: 130 1238 2840 2912 4655 4753 5438 130
Size: 1108 1602 72 1743 98 685 2398
Fragments arranged by size:
2398 1743 1602 1108 685 98 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7706
PvuII CAG'CTG
Cuts at: 483 2301 3525 3965 5131 5491 483
Size: 1818 1224 440 1166 360 2698
Fragments arranged by size:
2698 1818 1224 1166 440 360
RsrII CG'GwC_CG
Cuts at: 5901 5901
Size: 7706
SacI G_AGCT'C
Cuts at: 4720 4720
Size: 7706
SalI G'TCGA_C
Cuts at: 4743 4743
Size: 7706
SapI GCTCTTCn'nnn_
Cuts at: 1588 5729 5939 1588
Size: 4141 210 3355
Fragments arranged by size:
4141 3355 210
SbfI CC_TGCA'GG
Cuts at: 4753 4753
Size: 7706
ScaI AGT'ACT
Cuts at: 1258 4288 7223 1258
Size: 3030 2935 1741
Fragments arranged by size:
3030 2935 1741
SexAI A'CCwGG_T
Cuts at: 3320 3320
Size: 7706
SfcI C'TryA_G
Cuts at: 33 126 1234 2836 2865 2908 4651 4671 4749
Size: 93 1108 1602 29 43 1743 20 78
Cuts at: 4749 5434 6427 6618 33
Size: 685 993 191 1121
Fragments arranged by size:
1743 1602 1121 1108 993 685 191 93
78 43 29 20
SfoI GGC'GCC
Cuts at: 2688 3929 4635 5138 5386 2688
Size: 1241 706 503 248 5008
Fragments arranged by size:
5008 1241 706 503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7706
SmaI CCC'GGG
Cuts at: 2446 4432 4728 2446
Size: 1986 296 5424
Fragments arranged by size:
5424 1986 296
SmlI C'TyrA_G
Cuts at: 2678 3535 3898 4861 5776 6268 6530 2678
Size: 857 363 963 915 492 262 3854
Fragments arranged by size:
3854 963 915 857 492 363 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7706
SphI G_CATG'C
Cuts at: 2808 4759 5259 2808
Size: 1951 500 5255
Fragments arranged by size:
5255 1951 500
SrfI GCCC'GGGC
Cuts at: 2446 4432 2446
Size: 1986 5720
StuI AGG'CCT
Cuts at: 2251 2335 3670 2251
Size: 84 1335 6287
Fragments arranged by size:
6287 1335 84
StyI C'CwwG_G
Cuts at: 107 335 944 974 1907 2546 3209 3338 3471
Size: 228 609 30 933 639 663 129 133
Cuts at: 3471 4840 5194 107
Size: 1369 354 2619
Fragments arranged by size:
2619 1369 933 663 639 609 354 228
133 129 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 216 305 3300 3531 5289 5422 5881 6090 216
Size: 89 2995 231 1758 133 459 209 1832
Fragments arranged by size:
2995 1832 1758 459 231 209 133 89
TatI w'GTAC_w
Cuts at: 935 1256 2453 2993 4286 7221 935
Size: 321 1197 540 1293 2935 1420
Fragments arranged by size:
2935 1420 1293 1197 540 321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1505 1537 3297 3329 7223 7255 1505
Size: 32 1760 32 3894 32 1956
Fragments arranged by size:
3894 1956 1760 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5503 5503
Size: 7706
XbaI T'CTAG_A
Cuts at: 4737 4737
Size: 7706
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 1028 2249 3318 3699 4482 5201 333
Size: 695 1221 1069 381 783 719 2838
Fragments arranged by size:
2838 1221 1069 783 719 695 381
XhoI C'TCGA_G
Cuts at: 2678 3535 2678
Size: 857 6849
XmaI C'CCGG_G
Cuts at: 2444 4430 4726 2444
Size: 1986 296 5424
Fragments arranged by size:
5424 1986 296
XmnI GAAnn'nnTTC
Cuts at: 3331 4336 3331
Size: 1005 6701
ZraI GAC'GTC
Cuts at: 2616 4310 2616
Size: 1694 6012
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmtI BsiWI BsrGI
BssHII BstZ17I CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PsrI
SacII SanDI SfiI SgrAI SnaBI SspI SwaI