Restriction map for
pF1KB3797
(Circular) (Six-base) MAPSORT of: pF1KB3797.seq Check: 8906 from: 1 to: 4535
pF1KB3797 4535 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1573 1573
Size: 4535
Acc65I G'GTAC_C
Cuts at: 605 1551 605
Size: 946 3589
AclI AA'CG_TT
Cuts at: 1529 4375 1529
Size: 2846 1689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 902 2360 2792 3145 3807 353
Size: 549 1458 432 353 662 1081
Fragments arranged by size:
1458 1081 662 549 432 353
AflII C'TTAA_G
Cuts at: 820 820
Size: 4535
AflIII A'CryG_T
Cuts at: 1025 1368 1887 3687 3968 1025
Size: 343 519 1800 281 1592
Fragments arranged by size:
1800 1592 519 343 281
AlwNI CAG_nnn'CTG
Cuts at: 1840 3278 1840
Size: 1438 3097
ApaI G_GGCC'C
Cuts at: 1112 1112
Size: 4535
ApaLI G'TGCA_C
Cuts at: 496 3373 496
Size: 2877 1658
ApoI r'AATT_y
Cuts at: 1539 3888 4144 1539
Size: 2349 256 1930
Fragments arranged by size:
2349 1930 256
AseI AT'TA_AT
Cuts at: 20 905 20
Size: 885 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1219 1555 1219
Size: 336 4199
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 456 544 605 1551 1965 2213 2248 456
Size: 88 61 946 414 248 35 2743
Fragments arranged by size:
2743 946 414 248 88 61 35
BanII G_rGCy'C
Cuts at: 1112 1549 2579 1112
Size: 437 1030 3068
Fragments arranged by size:
3068 1030 437
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 1041 1041
Size: 4535
BbvCI CC'TCA_GC
Cuts at: 808 808
Size: 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 679 713 679
Size: 34 4501
BciVI GTATCCnnnnn_n'
Cuts at: 646 2433 3489 4535 646
Size: 1787 1056 1046 646
Fragments arranged by size:
1787 1056 1046 646
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 500 1112 2160 2253 3377 500
Size: 612 1048 93 1124 1658
Fragments arranged by size:
1658 1124 1048 612 93
BmrI ACTGGGnnnn_n'
Cuts at: 1474 1747 1828 2163 4307 1474
Size: 273 81 335 2144 1702
Fragments arranged by size:
2144 1702 335 273 81
BmtI G_CTAG'C
Cuts at: 1291 1291
Size: 4535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 1287 399
Size: 888 3647
Bpu10I CC'TnA_GC
Cuts at: 808 808
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1711 2590 3304 3602 1711
Size: 879 714 298 2644
Fragments arranged by size:
2644 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 255 897 1262 4239 255
Size: 642 365 2977 551
Fragments arranged by size:
2977 642 551 365
BsaAI yAC'GTr
Cuts at: 1888 1888
Size: 4535
BsaBI GATnn'nnATC
Cuts at: 6 877 2049 4086 6
Size: 871 1172 2037 455
Fragments arranged by size:
2037 1172 871 455
BsaHI Gr'CG_yC
Cuts at: 1966 2214 2916 4412 1966
Size: 248 702 1496 2089
Fragments arranged by size:
2089 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1724 2245 2923 3334 3481 3755 1724
Size: 521 678 411 147 274 2504
Fragments arranged by size:
2504 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 494 524 494
Size: 30 4505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1410 1410
Size: 4535
BseYI C'CCAG_C
Cuts at: 1324 1960 2718 3383 3791 1324
Size: 636 758 665 408 2068
Fragments arranged by size:
2068 758 665 636 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 612
Size: 4535
BsiEI CG_ry'CG
Cuts at: 104 2077 2123 3353 104
Size: 1973 46 1230 1286
Fragments arranged by size:
1973 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 500 1216 1549 2327 2517 3377 500
Size: 716 333 778 190 860 1658
Fragments arranged by size:
1658 860 778 716 333 190
BsmI GAATG_Cn'
Cuts at: 1452 1452
Size: 4535
BsmBI CGTCTCn'nnnn_
Cuts at: 214 1392 214
Size: 1178 3357
Bsp1286I G_dGCh'C
Cuts at: 500 1112 1216 1549 2160 2253 2327 2517 2579
Size: 612 104 333 611 93 74 190 62
Cuts at: 2579 3377 500
Size: 798 1658
Fragments arranged by size:
1658 798 612 611 333 190 104 93
74 62
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1585 1799 2101 2482 1585
Size: 214 302 381 3638
Fragments arranged by size:
3638 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 308 2447 308
Size: 2139 2396
BsrFI r'CCGG_y
Cuts at: 2533 2714 2533
Size: 181 4354
BssSI C'ACGA_G
Cuts at: 2806 3514 2806
Size: 708 3827
BstAPI GCAn_nnn'nTGC
Cuts at: 1912 1912
Size: 4535
BstBI TT'CG_AA
Cuts at: 2896 2896
Size: 4535
BstYI r'GATC_y
Cuts at: 1 1560 2027 2050 2385 2631 2937 2949 3035
Size: 1559 467 23 335 246 306 12 86
Cuts at: 3035 4087 1
Size: 1052 449
Fragments arranged by size:
1559 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 598 1393 598
Size: 795 3740
BtgI C'CryG_G
Cuts at: 284 284
Size: 4535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 704 2485 2666 2829 704
Size: 1781 181 163 2410
Fragments arranged by size:
2410 1781 181 163
BtsI GCAGTG_nn'
Cuts at: 1778 1778
Size: 4535
ClaI AT'CG_AT
Cuts at: 320 320
Size: 4535
DraI TTT'AAA
Cuts at: 416 1534 416
Size: 1118 3417
DraIII CAC_nnn'GTG
Cuts at: 504 504
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 2241 3585 2241
Size: 1344 3191
EaeI y'GGCC_r
Cuts at: 1238 1347 2120 2294 2685 2712 4057 1238
Size: 109 773 174 391 27 1345 1716
Fragments arranged by size:
1716 1345 773 391 174 109 27
EagI C'GGCC_G
Cuts at: 2120 2120
Size: 4535
EarI CTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2743 3485 3631 4345 2743
Size: 742 146 714 2933
Fragments arranged by size:
2933 742 714 146
EcoICRI GAG'CTC
Cuts at: 1547 1547
Size: 4535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 399 902 1287 2360 2792 3145 3807 353
Size: 46 503 385 1073 432 353 662 1081
Fragments arranged by size:
1081 1073 662 503 432 385 353 46
EcoNI CCTnn'n_nnAGG
Cuts at: 795 795
Size: 4535
EcoO109I rG'GnC_Cy
Cuts at: 277 1108 1109 1674 277
Size: 831 1 565 3138
Fragments arranged by size:
3138 831 565 1
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4535
FspI TGC'GCA
Cuts at: 179 2316 179
Size: 2137 2398
HaeII r_GCGC'y
Cuts at: 1969 2217 3447 4218 1969
Size: 248 1230 771 2286
Fragments arranged by size:
2286 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 551 583 1447 1479 2377 2407 2409
Size: 32 47 32 864 32 898 30 2
Cuts at: 2409 2439 472
Size: 30 2568
Fragments arranged by size:
2568 898 864 47 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1086 1179 1574 1086
Size: 93 395 4047
Fragments arranged by size:
4047 395 93
HindIII A'AGCT_T
Cuts at: 817 817
Size: 4535
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 609 1555 609
Size: 946 3589
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3294 3478 3294
Size: 184 4351
MscI TGG'CCA
Cuts at: 1240 2296 1240
Size: 1056 3479
MslI CAynn'nnrTG
Cuts at: 322 539 1024 2524 2651 322
Size: 217 485 1500 127 2206
Fragments arranged by size:
2206 1500 485 217 127
MspA1I CmG'CkG
Cuts at: 169 580 922 1132 1646 1960 2320 3104 3349
Size: 411 342 210 514 314 360 784 245
Cuts at: 3349 169
Size: 1355
Fragments arranged by size:
1355 784 514 411 360 342 314 245
210
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NheI G'CTAG_C
Cuts at: 1287 1287
Size: 4535
NspI r_CATG'y
Cuts at: 861 1029 1372 1588 2088 3691 861
Size: 168 343 216 500 1603 1705
Fragments arranged by size:
1705 1603 500 343 216 168
PciI A'CATG_T
Cuts at: 1025 1368 3687 1025
Size: 343 2319 1873
Fragments arranged by size:
2319 1873 343
PflMI CCAn_nnn'nTGG
Cuts at: 271 271
Size: 4535
PfoI T'CCnGG_A
Cuts at: 983 983
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 4503
PpuMI rG'GwC_Cy
Cuts at: 277 277
Size: 4535
PshAI GACnn'nnGTC
Cuts at: 254 254
Size: 4535
PspOMI G'GGCC_C
Cuts at: 1108 1108
Size: 4535
PstI C_TGCA'G
Cuts at: 1582 2267 1582
Size: 685 3850
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 580 922 1132 1960 2320 580
Size: 342 210 828 360 2795
Fragments arranged by size:
2795 828 360 342 210
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1549 1549
Size: 4535
SalI G'TCGA_C
Cuts at: 1572 1572
Size: 4535
SapI GCTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SfcI C'TryA_G
Cuts at: 33 1578 2263 3231 3422 33
Size: 1545 685 968 191 1146
Fragments arranged by size:
1545 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 820 1219 1690 2605 3319 3581 820
Size: 399 471 915 714 262 1774
Fragments arranged by size:
1774 915 714 471 399 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StyI C'CwwG_G
Cuts at: 632 1669 2023 632
Size: 1037 354 3144
Fragments arranged by size:
3144 1037 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 991 2118 2251 2710 2919 444
Size: 547 1127 133 459 209 2060
Fragments arranged by size:
2060 1127 547 459 209 133
TatI w'GTAC_w
Cuts at: 362 4050 362
Size: 3688 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4503
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 321 2030 321
Size: 1709 2826
XhoI C'TCGA_G
Cuts at: 1219 1219
Size: 4535
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BglI BmgBI BsiWI BspEI
BsrGI BssHII BstEII BstXI BstZ17I CspCI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NotI NruI NsiI
PacI PasI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI