Restriction map for
pF1KB3810
(Circular) (Six-base) MAPSORT of: pF1KB3810.seq Check: 858 from: 1 to: 4613
pF1KB3810 4613 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1651 1651
Size: 4613
Acc65I G'GTAC_C
Cuts at: 1629 1629
Size: 4613
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 448 850 1480 2438 2870 3223 3885 149
Size: 299 402 630 958 432 353 662 877
Fragments arranged by size:
958 877 662 630 432 402 353 299
AflIII A'CryG_T
Cuts at: 1965 3765 4046 1965
Size: 1800 281 2532
Fragments arranged by size:
2532 1800 281
AleI CACnn'nnGTG
Cuts at: 595 595
Size: 4613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 4613
AlwNI CAG_nnn'CTG
Cuts at: 1918 3356 1918
Size: 1438 3175
ApaLI G'TGCA_C
Cuts at: 724 761 3451 724
Size: 37 2690 1886
Fragments arranged by size:
2690 1886 37
ApoI r'AATT_y
Cuts at: 1617 3966 4222 1617
Size: 2349 256 2008
Fragments arranged by size:
2349 2008 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4613
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 1008 1633 1008
Size: 625 3988
BamHI G'GATC_C
Cuts at: 1260 1500 1638 1260
Size: 240 138 4235
Fragments arranged by size:
4235 240 138
BanI G'GyrC_C
Cuts at: 1629 2043 2291 2326 1629
Size: 414 248 35 3916
Fragments arranged by size:
3916 414 248 35
BanII G_rGCy'C
Cuts at: 905 1009 1627 2657 905
Size: 104 618 1030 2861
Fragments arranged by size:
2861 1030 618 104
BbeI G_GCGC'C
Cuts at: 2047 2295 2047
Size: 248 4365
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 614 648 614
Size: 34 4579
BciVI GTATCCnnnnn_n'
Cuts at: 2511 3567 4613 2511
Size: 1056 1046 2511
Fragments arranged by size:
2511 1056 1046
BclI T'GATC_A
Cuts at: 1028 2133 1028
Size: 1105 3508
BglII A'GATC_T
Cuts at: 2128 4165 2128
Size: 2037 2576
BlpI GC'TnA_GC
Cuts at: 1725 1725
Size: 4613
Bme1580I G_kGCm'C
Cuts at: 728 765 1211 2238 2331 3455 728
Size: 37 446 1027 93 1124 1886
Fragments arranged by size:
1886 1124 1027 446 93 37
BmrI ACTGGGnnnn_n'
Cuts at: 1825 1906 2241 4385 1825
Size: 81 335 2144 2053
Fragments arranged by size:
2144 2053 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 4613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 4613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1214 1789 2668 3382 3680 1214
Size: 575 879 714 298 2147
Fragments arranged by size:
2147 879 714 575 298
BsaI GGTCTCn'nnnn_
Cuts at: 1289 1532 4317 1289
Size: 243 2785 1585
Fragments arranged by size:
2785 1585 243
BsaAI yAC'GTr
Cuts at: 1966 1966
Size: 4613
BsaBI GATnn'nnATC
Cuts at: 6 2127 4164 6
Size: 2121 2037 455
Fragments arranged by size:
2121 2037 455
BsaHI Gr'CG_yC
Cuts at: 2044 2292 2994 4490 2044
Size: 248 702 1496 2167
Fragments arranged by size:
2167 1496 702 248
BsaWI w'CCGG_w
Cuts at: 413 707 909 1802 2323 3001 3412 3559 3833
Size: 294 202 893 521 678 411 147 274
Cuts at: 3833 413
Size: 1193
Fragments arranged by size:
1193 893 678 521 411 294 274 202
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 351 381 351
Size: 30 4583
BseYI C'CCAG_C
Cuts at: 1469 2038 2796 3461 3869 1469
Size: 569 758 665 408 2213
Fragments arranged by size:
2213 758 665 569 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 1310 1516 297
Size: 1013 206 3394
Fragments arranged by size:
3394 1013 206
BsiEI CG_ry'CG
Cuts at: 104 2155 2201 3431 104
Size: 2051 46 1230 1286
Fragments arranged by size:
2051 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 611 728 765 905 1009 1627 2405 2595 3455
Size: 117 37 140 104 618 778 190 860
Cuts at: 3455 611
Size: 1769
Fragments arranged by size:
1769 860 778 618 190 140 117 104
37
BsmBI CGTCTCn'nnnn_
Cuts at: 457 457
Size: 4613
Bsp1286I G_dGCh'C
Cuts at: 611 728 765 905 1009 1211 1627 2238 2331
Size: 117 37 140 104 202 416 611 93
Cuts at: 2331 2405 2595 2657 3455 611
Size: 74 190 62 798 1769
Fragments arranged by size:
1769 798 611 416 202 190 140 117
104 93 74 62 37
BspEI T'CCGG_A
Cuts at: 707 707
Size: 4613
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 880 1663 1877 2179 2560 880
Size: 783 214 302 381 2933
Fragments arranged by size:
2933 783 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2905 2959 4443 4608 50
Size: 2855 54 1484 165 55
Fragments arranged by size:
2855 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 882 2525 882
Size: 1643 2970
BsrFI r'CCGG_y
Cuts at: 683 2611 2792 683
Size: 1928 181 2504
Fragments arranged by size:
2504 1928 181
BssSI C'ACGA_G
Cuts at: 2884 3592 2884
Size: 708 3905
BstAPI GCAn_nnn'nTGC
Cuts at: 1990 1990
Size: 4613
BstBI TT'CG_AA
Cuts at: 2974 2974
Size: 4613
BstEII G'GTnAC_C
Cuts at: 550 550
Size: 4613
BstYI r'GATC_y
Cuts at: 1 1012 1260 1491 1500 1638 2105 2128 2463
Size: 1011 248 231 9 138 467 23 335
Cuts at: 2463 2709 3015 3027 3113 4165 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 1011 467 449 335 306 248 246
231 138 86 23 12 9
BtgI C'CryG_G
Cuts at: 107 107
Size: 4613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2563 2744 2907 2563
Size: 181 163 4269
Fragments arranged by size:
4269 181 163
BtsI GCAGTG_nn'
Cuts at: 1856 1856
Size: 4613
DraI TTT'AAA
Cuts at: 516 1388 1612 516
Size: 872 224 3517
Fragments arranged by size:
3517 872 224
DraIII CAC_nnn'GTG
Cuts at: 720 720
Size: 4613
DrdI GACnn_nn'nnGTC
Cuts at: 2319 3663 2319
Size: 1344 3269
EaeI y'GGCC_r
Cuts at: 110 2198 2372 2763 2790 4135 110
Size: 2088 174 391 27 1345 588
Fragments arranged by size:
2088 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2198 2198
Size: 4613
EarI CTCTTCn'nnn_
Cuts at: 802 1218 2636 2846 802
Size: 416 1418 210 2569
Fragments arranged by size:
2569 1418 416 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3563 3709 4423 2821
Size: 742 146 714 3011
Fragments arranged by size:
3011 742 714 146
EcoICRI GAG'CTC
Cuts at: 903 1007 1625 903
Size: 104 618 3891
Fragments arranged by size:
3891 618 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 448 625 850 1480 2438 2870 3223 3885
Size: 299 177 225 630 958 432 353 662
Cuts at: 3885 149
Size: 877
Fragments arranged by size:
958 877 662 630 432 353 299 225
177
EcoO109I rG'GnC_Cy
Cuts at: 287 1274 1752 287
Size: 987 478 3148
Fragments arranged by size:
3148 987 478
EcoRI G'AATT_C
Cuts at: 1617 1617
Size: 4613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4613
FspI TGC'GCA
Cuts at: 2394 2394
Size: 4613
HaeII r_GCGC'y
Cuts at: 2047 2295 3525 4296 2047
Size: 248 1230 771 2364
Fragments arranged by size:
2364 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 801 833 925 957 2455 2485 2487 2517 801
Size: 32 92 32 1498 30 2 30 2897
Fragments arranged by size:
2897 1498 92 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1652 1652
Size: 4613
HindIII A'AGCT_T
Cuts at: 505 1598 505
Size: 1093 3520
KasI G'GCGC_C
Cuts at: 2043 2291 2043
Size: 248 4365
KpnI G_GTAC'C
Cuts at: 1633 1633
Size: 4613
MluI A'CGCG_T
Cuts at: 4046 4046
Size: 4613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1238 3372 3556 1238
Size: 2134 184 2295
Fragments arranged by size:
2295 2134 184
MscI TGG'CCA
Cuts at: 112 2374 112
Size: 2262 2351
MslI CAynn'nnrTG
Cuts at: 595 721 2602 2729 595
Size: 126 1881 127 2479
Fragments arranged by size:
2479 1881 127 126
MspA1I CmG'CkG
Cuts at: 1724 2038 2398 3182 3427 1724
Size: 314 360 784 245 2910
Fragments arranged by size:
2910 784 360 314 245
NaeI GCC'GGC
Cuts at: 2794 2794
Size: 4613
NarI GG'CG_CC
Cuts at: 2044 2292 2044
Size: 248 4365
NcoI C'CATG_G
Cuts at: 107 107
Size: 4613
NdeI CA'TA_TG
Cuts at: 800 1128 800
Size: 328 4285
NgoMIV G'CCGG_C
Cuts at: 2792 2792
Size: 4613
NspI r_CATG'y
Cuts at: 538 1058 1666 2166 3769 538
Size: 520 608 500 1603 1382
Fragments arranged by size:
1603 1382 608 520 500
PciI A'CATG_T
Cuts at: 3765 3765
Size: 4613
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 4581
PpuMI rG'GwC_Cy
Cuts at: 1274 1274
Size: 4613
PstI C_TGCA'G
Cuts at: 1660 2345 1660
Size: 685 3928
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 2038 2398 2038
Size: 360 4253
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 905 1009 1627 905
Size: 104 618 3891
Fragments arranged by size:
3891 618 104
SalI G'TCGA_C
Cuts at: 1650 1650
Size: 4613
SanDI GG'GwC_CC
Cuts at: 1274 1274
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 1660 1660
Size: 4613
ScaI AGT'ACT
Cuts at: 4130 4130
Size: 4613
SfcI C'TryA_G
Cuts at: 33 1514 1656 2341 3309 3500 33
Size: 1481 142 685 968 191 1146
Fragments arranged by size:
1481 1146 968 685 191 142
SfoI GGC'GCC
Cuts at: 2045 2293 2045
Size: 248 4365
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 1635 1635
Size: 4613
SmlI C'TyrA_G
Cuts at: 1229 1768 2683 3397 3659 1229
Size: 539 915 714 262 2183
Fragments arranged by size:
2183 915 714 539 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 538 1666 2166 538
Size: 1128 500 2985
Fragments arranged by size:
2985 1128 500
StuI AGG'CCT
Cuts at: 232 322 232
Size: 90 4523
StyI C'CwwG_G
Cuts at: 107 317 1425 1747 2101 107
Size: 210 1108 322 354 2619
Fragments arranged by size:
2619 1108 354 322 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2196 2329 2788 2997 2196
Size: 133 459 209 3812
Fragments arranged by size:
3812 459 209 133
TatI w'GTAC_w
Cuts at: 335 4128 335
Size: 3793 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 105 137 1065 1097 4130 4162 105
Size: 32 928 32 3033 32 556
Fragments arranged by size:
3033 928 556 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2410 2410
Size: 4613
XbaI T'CTAG_A
Cuts at: 1644 1644
Size: 4613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2108
Size: 4613
XmaI C'CCGG_G
Cuts at: 1633 1633
Size: 4613
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI