Restriction map for
pF1KB3816
(Circular) (Six-base) MAPSORT of: pF1KB3816.seq Check: 4087 from: 1 to: 9149
pF1KB3816 9149 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1293 2119 1293
Size: 826 8323
AatII G_ACGT'C
Cuts at: 2435 2435
Size: 9149
AccI GT'mk_AC
Cuts at: 1550 3227 5444 6187 1550
Size: 1677 2217 743 4512
Fragments arranged by size:
4512 2217 1677 743
Acc65I G'GTAC_C
Cuts at: 281 1148 6165 281
Size: 867 5017 3265
Fragments arranged by size:
5017 3265 867
AclI AA'CG_TT
Cuts at: 4407 8989 4407
Size: 4582 4567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 413 904 3887 3963 5027 5938 6974 7406
Size: 50 491 2983 76 1064 911 1036 432
Cuts at: 7406 8153 8421 363
Size: 747 268 1091
Fragments arranged by size:
2983 1091 1064 1036 911 747 491 432
268 76 50
AfeI AGC'GCT
Cuts at: 851 851
Size: 9149
AflIII A'CryG_T
Cuts at: 1826 1835 4578 5630 6501 7605 8582 1826
Size: 9 2743 1052 871 1104 977 2393
Fragments arranged by size:
2743 2393 1104 1052 977 871 9
AgeI A'CCGG_T
Cuts at: 7537 7537
Size: 9149
AhdI GACnn_n'nnGTC
Cuts at: 1281 4088 1281
Size: 2807 6342
AleI CACnn'nnGTG
Cuts at: 1682 4579 1682
Size: 2897 6252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1275 3616 1275
Size: 2341 6808
AlwNI CAG_nnn'CTG
Cuts at: 1047 1126 3058 3603 3884 4554 5073 6454 8021
Size: 79 1932 545 281 670 519 1381 1567
Cuts at: 8021 1047
Size: 2175
Fragments arranged by size:
2175 1932 1567 1381 670 545 519 281
79
ApaI G_GGCC'C
Cuts at: 4483 5258 5327 4483
Size: 775 69 8305
Fragments arranged by size:
8305 775 69
ApaLI G'TGCA_C
Cuts at: 7919 7919
Size: 9149
ApoI r'AATT_y
Cuts at: 353 2278 2462 3464 3848 6153 8502 8758 353
Size: 1925 184 1002 384 2305 2349 256 744
Fragments arranged by size:
2349 2305 1925 1002 744 384 256 184
AseI AT'TA_AT
Cuts at: 20 2711 20
Size: 2691 6458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9149
AvaI C'yCGr_G
Cuts at: 316 853 3358 6092 6169 316
Size: 537 2505 2734 77 3296
Fragments arranged by size:
3296 2734 2505 537 77
BamHI G'GATC_C
Cuts at: 320 6174 320
Size: 5854 3295
BanI G'GyrC_C
Cuts at: 237 281 857 1148 5857 6165 6579 6827 6862
Size: 44 576 291 4709 308 414 248 35
Cuts at: 6862 237
Size: 2524
Fragments arranged by size:
4709 2524 576 414 308 291 248 44
35
BanII G_rGCy'C
Cuts at: 482 2065 2762 3020 3032 4483 4532 4886 5103
Size: 1583 697 258 12 1451 49 354 217
Cuts at: 5103 5258 5327 5942 6163 7193 482
Size: 155 69 615 221 1030 2438
Fragments arranged by size:
2438 1583 1451 1030 697 615 354 258
221 217 155 69 49 12
BbeI G_GCGC'C
Cuts at: 6583 6831 6583
Size: 248 8901
BbsI GAAGACnn'nnnn_
Cuts at: 167 525 931 1938 2390 4169 4274 167
Size: 358 406 1007 452 1779 105 5042
Fragments arranged by size:
5042 1779 1007 452 406 358 105
BbvCI CC'TCA_GC
Cuts at: 2246 2927 3708 6023 2246
Size: 681 781 2315 5372
Fragments arranged by size:
5372 2315 781 681
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 834 868 834
Size: 34 9115
BciVI GTATCCnnnnn_n'
Cuts at: 107 4333 4921 7047 7808 9149 107
Size: 4226 588 2126 761 1341 107
Fragments arranged by size:
4226 2126 1341 761 588 107
BclI T'GATC_A
Cuts at: 1481 2363 3806 6669 1481
Size: 882 1443 2863 3961
Fragments arranged by size:
3961 2863 1443 882
BfrBI ATG'CAT
Cuts at: 193 193
Size: 9149
BglII A'GATC_T
Cuts at: 302 3644 6664 8701 302
Size: 3342 3020 2037 750
Fragments arranged by size:
3342 3020 2037 750
BlpI GC'TnA_GC
Cuts at: 5102 5192 6261 5102
Size: 90 1069 7990
Fragments arranged by size:
7990 1069 90
Bme1580I G_kGCm'C
Cuts at: 242 860 3371 4483 5258 5327 6774 6867 7923
Size: 618 2511 1112 775 69 1447 93 1056
Cuts at: 7923 242
Size: 1468
Fragments arranged by size:
2511 1468 1447 1112 1056 775 618 93
69
BmgBI CAC'GTC
Cuts at: 330 1487 4280 5633 330
Size: 1157 2793 1353 3846
Fragments arranged by size:
3846 2793 1353 1157
BmrI ACTGGGnnnn_n'
Cuts at: 2298 3203 3365 3501 4082 4209 4527 5261 6361
Size: 905 162 136 581 127 318 734 1100
Cuts at: 6361 6442 6777 8921 2298
Size: 81 335 2144 2526
Fragments arranged by size:
2526 2144 1100 905 734 581 335 318
162 136 127 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 7516 4706
Size: 2810 6339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 1506 3106 3454 3577 4331 5430 5670 5956
Size: 1350 1600 348 123 754 1099 240 286
Cuts at: 5956 156
Size: 3349
Fragments arranged by size:
3349 1600 1350 1099 754 348 286 240
123
Bpu10I CC'TnA_GC
Cuts at: 863 1013 2246 2815 2927 3708 5872 5877 6023
Size: 150 1233 569 112 781 2164 5 146
Cuts at: 6023 863
Size: 3989
Fragments arranged by size:
3989 2164 1233 781 569 150 146 112
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1435 2088 3730 4870 6325 7204 7696 7994
Size: 161 653 1642 1140 1455 879 492 298
Cuts at: 7994 1274
Size: 2429
Fragments arranged by size:
2429 1642 1455 1140 879 653 492 298
161
BsaI GGTCTCn'nnnn_
Cuts at: 1971 5128 5517 8853 1971
Size: 3157 389 3336 2267
Fragments arranged by size:
3336 3157 2267 389
BsaAI yAC'GTr
Cuts at: 1035 1424 1829 1878 6502 1035
Size: 389 405 49 4624 3682
Fragments arranged by size:
4624 3682 405 389 49
BsaBI GATnn'nnATC
Cuts at: 6 6663 8700 6
Size: 6657 2037 455
Fragments arranged by size:
6657 2037 455
BsaHI Gr'CG_yC
Cuts at: 2432 6580 6828 7530 9026 2432
Size: 4148 248 702 1496 2555
Fragments arranged by size:
4148 2555 1496 702 248
BsaWI w'CCGG_w
Cuts at: 718 2999 6338 6859 7537 7811 7958 8369 718
Size: 2281 3339 521 678 274 147 411 1498
Fragments arranged by size:
3339 2281 1498 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 1813 1843 2627 2657 2950 2980 3614
Size: 30 1629 30 784 30 293 30 634
Cuts at: 3614 3644 4812 4842 5712 5741 5742 5771 154
Size: 30 1168 30 870 29 1 29 3532
Fragments arranged by size:
3532 1629 1168 870 784 634 293 30
30 30 30 30 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 543 4128 4974 5520 5952 5961 313
Size: 230 3585 846 546 432 9 3501
Fragments arranged by size:
3585 3501 846 546 432 230 9
BseYI C'CCAG_C
Cuts at: 765 820 2293 2567 3019 3516 4399 5320 6042
Size: 55 1473 274 452 497 883 921 722
Cuts at: 6042 6574 7332 7909 8405 765
Size: 532 758 577 496 1509
Fragments arranged by size:
1509 1473 921 883 758 722 577 532
497 496 452 274 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 1542 2517 3319 3947 1321
Size: 221 975 802 628 6523
Fragments arranged by size:
6523 975 802 628 221
BsiEI CG_ry'CG
Cuts at: 104 5284 6691 6737 7945 104
Size: 5180 1407 46 1208 1308
Fragments arranged by size:
5180 1407 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2065 2147 3032 4943 6163 6941 7131 7923 2065
Size: 82 885 1911 1220 778 190 792 3291
Fragments arranged by size:
3291 1911 1220 885 792 778 190 82
BsmI GAATG_Cn'
Cuts at: 1625 2875 5084 5316 1625
Size: 1250 2209 232 5458
Fragments arranged by size:
5458 2209 1250 232
BsmBI CGTCTCn'nnnn_
Cuts at: 585 718 3617 585
Size: 133 2899 6117
Fragments arranged by size:
6117 2899 133
Bsp1286I G_dGCh'C
Cuts at: 242 482 860 2065 2147 2762 3020 3032 3371
Size: 240 378 1205 82 615 258 12 339
Cuts at: 3371 4483 4532 4886 4943 5103 5258 5327 5942
Size: 1112 49 354 57 160 155 69 615
Cuts at: 5942 6163 6774 6867 6941 7131 7193 7923 242
Size: 221 611 93 74 190 62 730 1468
Fragments arranged by size:
1468 1205 1112 730 615 615 611 378
354 339 258 240 221 190 160 155
93 82 74 69 62 57 49 12
BspEI T'CCGG_A
Cuts at: 8369 8369
Size: 9149
BspHI T'CATG_A
Cuts at: 5021 8325 5021
Size: 3304 5845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1293 1742 2119 4727 5705 6199 6413 6715 7096
Size: 449 377 2608 978 494 214 302 381
Cuts at: 7096 1293
Size: 3346
Fragments arranged by size:
3346 2608 978 494 449 381 377 302
214
BsrBI CCG'CTC
Cuts at: 50 1288 7441 7495 8979 9144 50
Size: 1238 6153 54 1484 165 55
Fragments arranged by size:
6153 1484 1238 165 55 54
BsrDI GCAATG_nn'
Cuts at: 528 1161 2355 4184 7061 528
Size: 633 1194 1829 2877 2616
Fragments arranged by size:
2877 2616 1829 1194 633
BsrFI r'CCGG_y
Cuts at: 1374 2009 3316 7147 7328 7537 1374
Size: 635 1307 3831 181 209 2986
Fragments arranged by size:
3831 2986 1307 635 209 181
BsrGI T'GTAC_A
Cuts at: 663 663
Size: 9149
BssSI C'ACGA_G
Cuts at: 2058 5976 7420 7778 2058
Size: 3918 1444 358 3429
Fragments arranged by size:
3918 3429 1444 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2664 5073 6526 2664
Size: 2409 1453 5287
Fragments arranged by size:
5287 2409 1453
BstBI TT'CG_AA
Cuts at: 7510 7510
Size: 9149
BstEII G'GTnAC_C
Cuts at: 2034 2251 3175 4896 5794 2034
Size: 217 924 1721 898 5389
Fragments arranged by size:
5389 1721 924 898 217
BstXI CCAn_nnnn'nTGG
Cuts at: 2184 2631 3504 5934 5949 2184
Size: 447 873 2430 15 5384
Fragments arranged by size:
5384 2430 873 447 15
BstYI r'GATC_y
Cuts at: 1 302 320 616 3644 4204 6174 6641 6664
Size: 301 18 296 3028 560 1970 467 23
Cuts at: 6664 6999 7245 8257 8343 8355 8701 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
3028 1970 1012 560 467 449 346 335
301 296 246 86 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 3071 4518 5741 3071
Size: 1447 1223 6479
Fragments arranged by size:
6479 1447 1223
BtgI C'CryG_G
Cuts at: 224 290 638 815 2194 2730 2825 3159 3683
Size: 66 348 177 1379 536 95 334 524
Cuts at: 3683 3716 4000 5153 5927 6008 224
Size: 33 284 1153 774 81 3365
Fragments arranged by size:
3365 1379 1153 774 536 524 348 334
284 177 95 81 66 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 790 2485 7099 7280 7443 790
Size: 1695 4614 181 163 2496
Fragments arranged by size:
4614 2496 1695 181 163
BtsI GCAGTG_nn'
Cuts at: 1559 3930 4381 5720 6079 6392 1559
Size: 2371 451 1339 359 313 4316
Fragments arranged by size:
4316 2371 1339 451 359 313
ClaI AT'CG_AT
Cuts at: 711 711
Size: 9149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2931 2966 2931
Size: 35 9114
DraI TTT'AAA
Cuts at: 4223 6148 4223
Size: 1925 7224
DraIII CAC_nnn'GTG
Cuts at: 5615 5615
Size: 9149
DrdI GACnn_nn'nnGTC
Cuts at: 1546 6855 7713 1546
Size: 5309 858 2982
Fragments arranged by size:
5309 2982 858
EaeI y'GGCC_r
Cuts at: 2007 2011 2328 2501 2939 5281 5549 5788 6011
Size: 4 317 173 438 2342 268 239 223
Cuts at: 6011 6734 6908 7299 7326 8671 2007
Size: 723 174 391 27 1345 2485
Fragments arranged by size:
2485 2342 1345 723 438 391 317 268
239 223 174 173 27 4
EagI C'GGCC_G
Cuts at: 5281 6734 5281
Size: 1453 7696
EarI CTCTTCn'nnn_
Cuts at: 2633 3906 5111 5573 5748 7172 7382 2633
Size: 1273 1205 462 175 1424 210 4400
Fragments arranged by size:
4400 1424 1273 1205 462 210 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 313 1883 3058 4125 5955 7357 7667 7813 8959
Size: 1570 1175 1067 1830 1402 310 146 1146
Cuts at: 8959 313
Size: 503
Fragments arranged by size:
1830 1570 1402 1175 1146 1067 503 310
146
EcoICRI GAG'CTC
Cuts at: 2063 3030 6161 2063
Size: 967 3131 5051
Fragments arranged by size:
5051 3131 967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 363 413 904 1506 3106 3454 3577 3887
Size: 207 50 491 602 1600 348 123 310
Cuts at: 3887 3963 4331 5027 5430 5670 5938 5956 6974
Size: 76 368 696 403 240 268 18 1018
Cuts at: 6974 7406 8153 8421 156
Size: 432 747 268 884
Fragments arranged by size:
1600 1018 884 747 696 602 491 432
403 368 348 310 268 268 240 207
123 76 50 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1591 2404 1591
Size: 813 8336
EcoO109I rG'GnC_Cy
Cuts at: 1615 3325 3676 4041 4649 4685 5255 5555 5868
Size: 1710 351 365 608 36 570 300 313
Cuts at: 5868 6288 1615
Size: 420 4476
Fragments arranged by size:
4476 1710 608 570 420 365 351 313
300 36
EcoRI G'AATT_C
Cuts at: 3464 3848 6153 3464
Size: 384 2305 6460
Fragments arranged by size:
6460 2305 384
EcoRV GAT'ATC
Cuts at: 2955 2955
Size: 9149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3471 7168 3471
Size: 3697 5452
FseI GG_CCGG'CC
Cuts at: 2013 2013
Size: 9149
FspI TGC'GCA
Cuts at: 1270 6930 1270
Size: 5660 3489
HaeII r_GCGC'y
Cuts at: 746 853 5925 6583 6831 7853 8832 746
Size: 107 5072 658 248 1022 979 1063
Fragments arranged by size:
5072 1063 1022 979 658 248 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 154 186 614 646 1267 1299 1680
Size: 32 17 32 428 32 621 32 381
Cuts at: 1680 1712 2064 2096 2637 2669 3079 3111 3614
Size: 32 352 32 541 32 410 32 503
Cuts at: 3614 3646 3852 3884 4074 4106 4662 4694 5134
Size: 32 206 32 190 32 556 32 440
Cuts at: 5134 5166 5199 5231 5620 5652 6991 7021 7023
Size: 32 33 32 389 32 1339 30 2
Cuts at: 7023 7053 105
Size: 30 2201
Fragments arranged by size:
2201 1339 621 556 541 503 440 428
410 389 381 352 206 190 33 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 30
17 2
HincII GTy'rAC
Cuts at: 3228 5165 5648 6188 3228
Size: 1937 483 540 6189
Fragments arranged by size:
6189 1937 540 483
HindIII A'AGCT_T
Cuts at: 375 4134 375
Size: 3759 5390
KasI G'GCGC_C
Cuts at: 6579 6827 6579
Size: 248 8901
KpnI G_GTAC'C
Cuts at: 285 1152 6169 285
Size: 867 5017 3265
Fragments arranged by size:
5017 3265 867
MfeI C'AATT_G
Cuts at: 2235 2315 2235
Size: 80 9069
MluI A'CGCG_T
Cuts at: 4578 8582 4578
Size: 4004 5145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1019 1469 7820 8004 1019
Size: 450 6351 184 2164
Fragments arranged by size:
6351 2164 450 184
MscI TGG'CCA
Cuts at: 2503 5551 6013 6910 2503
Size: 3048 462 897 4742
Fragments arranged by size:
4742 3048 897 462
MslI CAynn'nnrTG
Cuts at: 1257 1682 2983 3598 4579 5947 7138 7265 1257
Size: 425 1301 615 981 1368 1191 127 3141
Fragments arranged by size:
3141 1368 1301 1191 981 615 425 127
MspA1I CmG'CkG
Cuts at: 418 695 878 1556 1941 2137 2567 2931 3236
Size: 277 183 678 385 196 430 364 305
Cuts at: 3236 3685 3700 4181 6027 6260 6574 6934 7947
Size: 449 15 481 1846 233 314 360 1013
Cuts at: 7947 8192 418
Size: 245 1375
Fragments arranged by size:
1846 1375 1013 678 481 449 430 385
364 360 314 305 277 245 233 196
183 15
NaeI GCC'GGC
Cuts at: 2011 3318 7330 2011
Size: 1307 4012 3830
Fragments arranged by size:
4012 3830 1307
NarI GG'CG_CC
Cuts at: 6580 6828 6580
Size: 248 8901
NcoI C'CATG_G
Cuts at: 815 2825 3716 4000 815
Size: 2010 891 284 5964
Fragments arranged by size:
5964 2010 891 284
NgoMIV G'CCGG_C
Cuts at: 2009 3316 7328 2009
Size: 1307 4012 3830
Fragments arranged by size:
4012 3830 1307
NruI TCG'CGA
Cuts at: 145 145
Size: 9149
NsiI A_TGCA'T
Cuts at: 195 195
Size: 9149
NspI r_CATG'y
Cuts at: 897 2374 2988 5891 6202 6702 7609 897
Size: 1477 614 2903 311 500 907 2437
Fragments arranged by size:
2903 2437 1477 907 614 500 311
PasI CC'CwG_GG
Cuts at: 2020 4483 5096 5426 2020
Size: 2463 613 330 5743
Fragments arranged by size:
5743 2463 613 330
PciI A'CATG_T
Cuts at: 7605 7605
Size: 9149
PflMI CCAn_nnn'nTGG
Cuts at: 1715 2020 2273 3603 4667 5354 5408 1715
Size: 305 253 1330 1064 687 54 5456
Fragments arranged by size:
5456 1330 1064 687 305 253 54
PfoI T'CCnGG_A
Cuts at: 1535 5933 1535
Size: 4398 4751
PmeI GTTT'AAAC
Cuts at: 6148 6148
Size: 9149
PmlI CAC'GTG
Cuts at: 1424 1829 1878 1424
Size: 405 49 8695
Fragments arranged by size:
8695 405 49
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4519 4551 8288 8320 4519
Size: 32 3737 32 5348
Fragments arranged by size:
5348 3737 32 32
PpuMI rG'GwC_Cy
Cuts at: 3676 4041 4685 5868 3676
Size: 365 644 1183 6957
Fragments arranged by size:
6957 1183 644 365
PsiI TTA'TAA
Cuts at: 1219 1219
Size: 9149
PspOMI G'GGCC_C
Cuts at: 4479 5254 5323 4479
Size: 775 69 8305
Fragments arranged by size:
8305 775 69
PstI C_TGCA'G
Cuts at: 1114 2660 4056 4352 4724 4748 5436 5716 6196
Size: 1546 1396 296 372 24 688 280 480
Cuts at: 6196 6881 1114
Size: 685 3382
Fragments arranged by size:
3382 1546 1396 688 685 480 372 296
280 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9149
PvuII CAG'CTG
Cuts at: 418 2931 4181 6574 6934 418
Size: 2513 1250 2393 360 2633
Fragments arranged by size:
2633 2513 2393 1250 360
RsrII CG'GwC_CG
Cuts at: 7344 7344
Size: 9149
SacI G_AGCT'C
Cuts at: 2065 3032 6163 2065
Size: 967 3131 5051
Fragments arranged by size:
5051 3131 967
SacII CC_GC'GG
Cuts at: 3686 3686
Size: 9149
SalI G'TCGA_C
Cuts at: 3226 6186 3226
Size: 2960 6189
SanDI GG'GwC_CC
Cuts at: 4041 4041
Size: 9149
SapI GCTCTTCn'nnn_
Cuts at: 5573 7172 7382 5573
Size: 1599 210 7340
Fragments arranged by size:
7340 1599 210
SbfI CC_TGCA'GG
Cuts at: 4724 6196 4724
Size: 1472 7677
ScaI AGT'ACT
Cuts at: 4354 8666 4354
Size: 4312 4837
SexAI A'CCwGG_T
Cuts at: 4732 4732
Size: 9149
SfcI C'TryA_G
Cuts at: 33 1110 2656 2973 3588 3843 4052 4348 4720
Size: 1077 1546 317 615 255 209 296 372
Cuts at: 4720 4744 5432 5712 6192 6877 7870 8061 33
Size: 24 688 280 480 685 993 191 1121
Fragments arranged by size:
1546 1121 1077 993 688 685 615 480
372 317 296 280 255 209 191 24
SfoI GGC'GCC
Cuts at: 6581 6829 6581
Size: 248 8901
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9149
SmaI CCC'GGG
Cuts at: 318 6094 6171 318
Size: 5776 77 3296
Fragments arranged by size:
5776 3296 77
SmlI C'TyrA_G
Cuts at: 1289 1450 2067 3358 3745 4885 6304 7219 7711
Size: 161 617 1291 387 1140 1419 915 492
Cuts at: 7711 7973 1289
Size: 262 2465
Fragments arranged by size:
2465 1419 1291 1140 915 617 492 387
262 161
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9149
SphI G_CATG'C
Cuts at: 5891 6202 6702 5891
Size: 311 500 8338
Fragments arranged by size:
8338 500 311
SspI AAT'ATT
Cuts at: 2049 2049
Size: 9149
StuI AGG'CCT
Cuts at: 3304 3823 3304
Size: 519 8630
StyI C'CwwG_G
Cuts at: 815 2028 2624 2825 3446 3716 4000 5315 6283
Size: 1213 596 201 621 270 284 1315 968
Cuts at: 6283 6637 815
Size: 354 3327
Fragments arranged by size:
3327 1315 1213 968 621 596 354 284
270 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2597 3508 3876 4101 5439 6732 6865 7324 7533
Size: 911 368 225 1338 1293 133 459 209
Cuts at: 7533 2597
Size: 4213
Fragments arranged by size:
4213 1338 1293 911 459 368 225 209
133
TatI w'GTAC_w
Cuts at: 663 1823 3185 3578 3770 3875 4233 4352 4643
Size: 1160 1362 393 192 105 358 119 291
Cuts at: 4643 4819 5666 8664 663
Size: 176 847 2998 1148
Fragments arranged by size:
2998 1362 1160 1148 847 393 358 291
192 176 119 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1047 1079 1169 1201 1813 1845 2627 2659 2950
Size: 32 90 32 612 32 782 32 291
Cuts at: 2950 2982 3144 3176 5712 5744 8666 8698 1047
Size: 32 162 32 2536 32 2922 32 1498
Fragments arranged by size:
2922 2536 1498 782 612 291 162 90
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3628 4015 4278 6946 3628
Size: 387 263 2668 5831
Fragments arranged by size:
5831 2668 387 263
XbaI T'CTAG_A
Cuts at: 619 6180 619
Size: 5561 3588
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 231 875 2628 6644 114
Size: 117 644 1753 4016 2619
Fragments arranged by size:
4016 2619 1753 644 117
XhoI C'TCGA_G
Cuts at: 3358 3358
Size: 9149
XmaI C'CCGG_G
Cuts at: 316 6092 6169 316
Size: 5776 77 3296
Fragments arranged by size:
5776 3296 77
XmnI GAAnn'nnTTC
Cuts at: 151 3274 151
Size: 3123 6026
ZraI GAC'GTC
Cuts at: 2433 2433
Size: 9149
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BglI BmtI BsiWI
BssHII BstZ17I FspAI HpaI NdeI NheI NotI PacI
PshAI PspXI PsrI SfiI SgrAI SnaBI SrfI SwaI