Restriction map for
pF1KB3831
(Circular) (Six-base) MAPSORT of: pF1KB3831.seq Check: 2811 from: 1 to: 5216
pF1KB3831 5216 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 501 806 501
Size: 305 4911
AatII G_ACGT'C
Cuts at: 326 326
Size: 5216
AccI GT'mk_AC
Cuts at: 2254 2254
Size: 5216
Acc65I G'GTAC_C
Cuts at: 2232 2232
Size: 5216
AclI AA'CG_TT
Cuts at: 5056 5056
Size: 5216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 468 3041 3473 3826 4488 220
Size: 248 2573 432 353 662 948
Fragments arranged by size:
2573 948 662 432 353 248
AfeI AGC'GCT
Cuts at: 1048 1048
Size: 5216
AflIII A'CryG_T
Cuts at: 887 2568 4368 4649 887
Size: 1681 1800 281 1454
Fragments arranged by size:
1800 1681 1454 281
AhdI GACnn_n'nnGTC
Cuts at: 327 327
Size: 5216
AleI CACnn'nnGTG
Cuts at: 1018 1372 1018
Size: 354 4862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1657 2053 1657
Size: 396 4820
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2119 2151 2119
Size: 32 5184
AlwNI CAG_nnn'CTG
Cuts at: 264 738 1236 1330 2004 2521 3959 264
Size: 474 498 94 674 517 1438 1521
Fragments arranged by size:
1521 1438 674 517 498 474 94
ApaI G_GGCC'C
Cuts at: 833 1467 1891 2025 2107 833
Size: 634 424 134 82 3942
Fragments arranged by size:
3942 634 424 134 82
ApaLI G'TGCA_C
Cuts at: 2146 4054 2146
Size: 1908 3308
ApoI r'AATT_y
Cuts at: 2220 4569 4825 2220
Size: 2349 256 2611
Fragments arranged by size:
2611 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5216
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
AvaI C'yCGr_G
Cuts at: 179 1056 2095 2107 2236 179
Size: 877 1039 12 129 3159
Fragments arranged by size:
3159 1039 877 129 12
BamHI G'GATC_C
Cuts at: 812 2241 812
Size: 1429 3787
BanI G'GyrC_C
Cuts at: 150 357 454 821 871 975 1398 1534 1914
Size: 207 97 367 50 104 423 136 380
Cuts at: 1914 2232 2646 2894 2929 150
Size: 318 414 248 35 2437
Fragments arranged by size:
2437 423 414 380 367 318 248 207
136 104 97 50 35
BanII G_rGCy'C
Cuts at: 530 833 947 1112 1467 1510 1712 1840 1891
Size: 303 114 165 355 43 202 128 51
Cuts at: 1891 2025 2107 2175 2230 3260 530
Size: 134 82 68 55 1030 2486
Fragments arranged by size:
2486 1030 355 303 202 165 134 128
114 82 68 55 51 43
BbeI G_GCGC'C
Cuts at: 875 2650 2898 875
Size: 1775 248 3193
Fragments arranged by size:
3193 1775 248
BbsI GAAGACnn'nnnn_
Cuts at: 1327 1327
Size: 5216
BbvCI CC'TCA_GC
Cuts at: 2120 2120
Size: 5216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 733 767 733
Size: 34 5182
BciVI GTATCCnnnnn_n'
Cuts at: 3114 4170 5216 3114
Size: 1056 1046 3114
Fragments arranged by size:
3114 1056 1046
BclI T'GATC_A
Cuts at: 2736 2736
Size: 5216
BglI GCCn_nnn'nGGC
Cuts at: 760 880 1159 1303 1613 1645 760
Size: 120 279 144 310 32 4331
Fragments arranged by size:
4331 310 279 144 120 32
BglII A'GATC_T
Cuts at: 2731 4768 2731
Size: 2037 3179
BlpI GC'TnA_GC
Cuts at: 856 1603 2328 856
Size: 747 725 3744
Fragments arranged by size:
3744 747 725
Bme1580I G_kGCm'C
Cuts at: 457 833 1467 1891 1919 2025 2107 2150 2841
Size: 376 634 424 28 106 82 43 691
Cuts at: 2841 2934 4058 457
Size: 93 1124 1615
Fragments arranged by size:
1615 1124 691 634 424 376 106 93
82 43 28
BmgBI CAC'GTC
Cuts at: 897 897
Size: 5216
BmrI ACTGGGnnnn_n'
Cuts at: 2428 2509 2844 4988 2428
Size: 81 335 2144 2656
Fragments arranged by size:
2656 2144 335 81
BmtI G_CTAG'C
Cuts at: 934 2047 934
Size: 1113 4103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1854 2110 3583 1854
Size: 256 1473 3487
Fragments arranged by size:
3487 1473 256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 2209 514
Size: 1695 3521
Bpu10I CC'TnA_GC
Cuts at: 122 1706 2120 122
Size: 1584 414 3218
Fragments arranged by size:
3218 1584 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2392 3271 3985 4283 2392
Size: 879 714 298 3325
Fragments arranged by size:
3325 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4920 4920
Size: 5216
BsaAI yAC'GTr
Cuts at: 2569 2569
Size: 5216
BsaBI GATnn'nnATC
Cuts at: 6 2204 2730 4767 6
Size: 2198 526 2037 455
Fragments arranged by size:
2198 2037 526 455
BsaHI Gr'CG_yC
Cuts at: 323 695 872 1586 2647 2895 3597 5093 323
Size: 372 177 714 1061 248 702 1496 446
Fragments arranged by size:
1496 1061 714 702 446 372 248 177
BsaWI w'CCGG_w
Cuts at: 2405 2926 3604 4015 4162 4436 2405
Size: 521 678 411 147 274 3185
Fragments arranged by size:
3185 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2119 2149 2119
Size: 30 5186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2099 2137 2140 2099
Size: 38 3 5175
Fragments arranged by size:
5175 38 3
BseYI C'CCAG_C
Cuts at: 171 705 801 2641 3399 4064 4472 171
Size: 534 96 1840 758 665 408 915
Fragments arranged by size:
1840 915 758 665 534 408 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 249 277 2179 237
Size: 12 28 1902 3274
Fragments arranged by size:
3274 1902 28 12
BsiEI CG_ry'CG
Cuts at: 104 1617 1928 2758 2804 4034 104
Size: 1513 311 830 46 1230 1286
Fragments arranged by size:
1513 1286 1230 830 311 46
BsiHKAI G_wGCw'C
Cuts at: 530 947 1993 2150 2230 3008 3198 4058 530
Size: 417 1046 157 80 778 190 860 1688
Fragments arranged by size:
1688 1046 860 778 417 190 157 80
BsmBI CGTCTCn'nnnn_
Cuts at: 1837 1837
Size: 5216
Bsp1286I G_dGCh'C
Cuts at: 457 530 833 947 1112 1467 1510 1712 1840
Size: 73 303 114 165 355 43 202 128
Cuts at: 1840 1891 1919 1993 2025 2107 2150 2175 2230
Size: 51 28 74 32 82 43 25 55
Cuts at: 2230 2841 2934 3008 3198 3260 4058 457
Size: 611 93 74 190 62 798 1615
Fragments arranged by size:
1615 798 611 355 303 202 190 165
128 114 93 82 74 74 73 62
55 51 43 43 32 28 25
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 5216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 501 806 2012 2266 2480 2782 3163 272
Size: 229 305 1206 254 214 302 381 2325
Fragments arranged by size:
2325 1206 381 305 302 254 229 214
BsrBI CCG'CTC
Cuts at: 50 3508 3562 5046 5211 50
Size: 3458 54 1484 165 55
Fragments arranged by size:
3458 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 379 3128 379
Size: 2749 2467
BsrFI r'CCGG_y
Cuts at: 147 153 538 1612 1927 1981 3214 3395 147
Size: 6 385 1074 315 54 1233 181 1968
Fragments arranged by size:
1968 1233 1074 385 315 181 54 6
BssSI C'ACGA_G
Cuts at: 225 1018 1156 2168 3487 4195 225
Size: 793 138 1012 1319 708 1246
Fragments arranged by size:
1319 1246 1012 793 708 138
BstAPI GCAn_nnn'nTGC
Cuts at: 264 914 2004 2593 264
Size: 650 1090 589 2887
Fragments arranged by size:
2887 1090 650 589
BstBI TT'CG_AA
Cuts at: 3577 3577
Size: 5216
BstEII G'GTnAC_C
Cuts at: 900 900
Size: 5216
BstXI CCAn_nnnn'nTGG
Cuts at: 1182 1314 1182
Size: 132 5084
BstYI r'GATC_y
Cuts at: 1 812 2241 2708 2731 3066 3312 3618 3630
Size: 811 1429 467 23 335 246 306 12
Cuts at: 3630 3716 4768 1
Size: 86 1052 449
Fragments arranged by size:
1429 1052 811 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 970 1097 970
Size: 127 5089
BtgI C'CryG_G
Cuts at: 107 301 473 755 817 986 1079 1142 1370
Size: 194 172 282 62 169 93 63 228
Cuts at: 1370 1489 1538 107
Size: 119 49 3785
Fragments arranged by size:
3785 282 228 194 172 169 119 93
63 62 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 354 3166 3347 3510 354
Size: 2812 181 163 2060
Fragments arranged by size:
2812 2060 181 163
BtsI GCAGTG_nn'
Cuts at: 237 736 1278 2459 237
Size: 499 542 1181 2994
Fragments arranged by size:
2994 1181 542 499
DraI TTT'AAA
Cuts at: 2215 2215
Size: 5216
DraIII CAC_nnn'GTG
Cuts at: 914 1941 914
Size: 1027 4189
DrdI GACnn_nn'nnGTC
Cuts at: 1347 1522 2922 4266 1347
Size: 175 1400 1344 2297
Fragments arranged by size:
2297 1400 1344 175
EaeI y'GGCC_r
Cuts at: 386 536 1010 1304 1541 1610 1614 1925 2801
Size: 150 474 294 237 69 4 311 876
Cuts at: 2801 2975 3366 3393 4738 386
Size: 174 391 27 1345 864
Fragments arranged by size:
1345 876 864 474 391 311 294 237
174 150 69 27 4
EagI C'GGCC_G
Cuts at: 1614 1925 2801 1614
Size: 311 876 4029
Fragments arranged by size:
4029 876 311
EarI CTCTTCn'nnn_
Cuts at: 3239 3449 3239
Size: 210 5006
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 708 1201 1515 3424 4166 4312 5026 708
Size: 493 314 1909 742 146 714 898
Fragments arranged by size:
1909 898 742 714 493 314 146
EcoICRI GAG'CTC
Cuts at: 528 945 2228 528
Size: 417 1283 3516
Fragments arranged by size:
3516 1283 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 468 514 2209 3041 3473 3826 4488 220
Size: 248 46 1695 832 432 353 662 948
Fragments arranged by size:
1695 948 832 662 432 353 248 46
EcoNI CCTnn'n_nnAGG
Cuts at: 1471 2098 1471
Size: 627 4589
EcoO109I rG'GnC_Cy
Cuts at: 261 684 761 829 1101 1463 1464 1482 1695
Size: 423 77 68 272 362 1 18 213
Cuts at: 1695 1864 1881 2001 2021 2022 2103 2104 2355
Size: 169 17 120 20 1 81 1 251
Cuts at: 2355 261
Size: 3122
Fragments arranged by size:
3122 423 362 272 251 213 169 120
81 77 68 20 18 17 1 1
1
EcoRI G'AATT_C
Cuts at: 2220 2220
Size: 5216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1782 3235 1782
Size: 1453 3763
FseI GG_CCGG'CC
Cuts at: 1616 1616
Size: 5216
FspI TGC'GCA
Cuts at: 1471 2997 1471
Size: 1526 3690
HaeII r_GCGC'y
Cuts at: 171 253 875 1050 1298 1367 1396 2650 2898
Size: 82 622 175 248 69 29 1254 248
Cuts at: 2898 4128 4899 171
Size: 1230 771 488
Fragments arranged by size:
1254 1230 771 622 488 248 248 175
82 69 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 3058 3088 3090 3120 313
Size: 32 2713 30 2 30 2409
Fragments arranged by size:
2713 2409 32 30 30 2
HincII GTy'rAC
Cuts at: 2255 2255
Size: 5216
KasI G'GCGC_C
Cuts at: 871 2646 2894 871
Size: 1775 248 3193
Fragments arranged by size:
3193 1775 248
KpnI G_GTAC'C
Cuts at: 2236 2236
Size: 5216
MluI A'CGCG_T
Cuts at: 4649 4649
Size: 5216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 183 3975 4159 183
Size: 3792 184 1240
Fragments arranged by size:
3792 1240 184
MscI TGG'CCA
Cuts at: 1012 1306 2977 1012
Size: 294 1671 3251
Fragments arranged by size:
3251 1671 294
MslI CAynn'nnrTG
Cuts at: 355 664 1006 1018 1372 1549 3205 3332 355
Size: 309 342 12 354 177 1656 127 2239
Fragments arranged by size:
2239 1656 354 342 309 177 127 12
MspA1I CmG'CkG
Cuts at: 221 303 534 819 981 1347 1404 1491 1540
Size: 82 231 285 162 366 57 87 49
Cuts at: 1540 1743 1924 1962 2133 2327 2641 3001 3785
Size: 203 181 38 171 194 314 360 784
Cuts at: 3785 4030 221
Size: 245 1407
Fragments arranged by size:
1407 784 366 360 314 285 245 231
203 194 181 171 162 87 82 57
49 38
NaeI GCC'GGC
Cuts at: 540 1614 1929 3397 540
Size: 1074 315 1468 2359
Fragments arranged by size:
2359 1468 1074 315
NarI GG'CG_CC
Cuts at: 872 2647 2895 872
Size: 1775 248 3193
Fragments arranged by size:
3193 1775 248
NcoI C'CATG_G
Cuts at: 107 755 107
Size: 648 4568
NgoMIV G'CCGG_C
Cuts at: 538 1612 1927 3395 538
Size: 1074 315 1468 2359
Fragments arranged by size:
2359 1468 1074 315
NheI G'CTAG_C
Cuts at: 930 2043 930
Size: 1113 4103
NspI r_CATG'y
Cuts at: 469 2269 2769 4372 469
Size: 1800 500 1603 1313
Fragments arranged by size:
1800 1603 1313 500
PasI CC'CwG_GG
Cuts at: 1872 2025 1872
Size: 153 5063
PciI A'CATG_T
Cuts at: 4368 4368
Size: 5216
PflMI CCAn_nnn'nTGG
Cuts at: 212 281 1941 212
Size: 69 1660 3487
Fragments arranged by size:
3487 1660 69
PmeI GTTT'AAAC
Cuts at: 2215 2215
Size: 5216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2151 3662 3694 2119
Size: 32 1511 32 3641
Fragments arranged by size:
3641 1511 32 32
PpuMI rG'GwC_Cy
Cuts at: 261 684 1101 1482 1881 2001 261
Size: 423 417 381 399 120 3476
Fragments arranged by size:
3476 423 417 399 381 120
PshAI GACnn'nnGTC
Cuts at: 1849 1849
Size: 5216
PspOMI G'GGCC_C
Cuts at: 829 1463 1887 2021 2103 829
Size: 634 424 134 82 3942
Fragments arranged by size:
3942 634 424 134 82
PspXI vC'TCGA_Gb
Cuts at: 1056 1056
Size: 5216
PstI C_TGCA'G
Cuts at: 134 398 569 1775 2000 2056 2263 2948 134
Size: 264 171 1206 225 56 207 685 2402
Fragments arranged by size:
2402 1206 685 264 225 207 171 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5216
PvuII CAG'CTG
Cuts at: 221 534 1347 2133 2641 3001 221
Size: 313 813 786 508 360 2436
Fragments arranged by size:
2436 813 786 508 360 313
RsrII CG'GwC_CG
Cuts at: 156 3411 156
Size: 3255 1961
SacI G_AGCT'C
Cuts at: 530 947 2230 530
Size: 417 1283 3516
Fragments arranged by size:
3516 1283 417
SacII CC_GC'GG
Cuts at: 304 820 1492 1541 304
Size: 516 672 49 3979
Fragments arranged by size:
3979 672 516 49
SalI G'TCGA_C
Cuts at: 2253 2253
Size: 5216
SapI GCTCTTCn'nnn_
Cuts at: 3239 3449 3239
Size: 210 5006
SbfI CC_TGCA'GG
Cuts at: 2263 2263
Size: 5216
ScaI AGT'ACT
Cuts at: 4733 4733
Size: 5216
SexAI A'CCwGG_T
Cuts at: 680 680
Size: 5216
SfcI C'TryA_G
Cuts at: 33 130 394 405 565 1417 1771 1782 1996
Size: 97 264 11 160 852 354 11 214
Cuts at: 1996 2052 2259 2944 3912 4103 33
Size: 56 207 685 968 191 1146
Fragments arranged by size:
1146 968 852 685 354 264 214 207
191 160 97 56 11 11
SfoI GGC'GCC
Cuts at: 873 2648 2896 873
Size: 1775 248 3193
Fragments arranged by size:
3193 1775 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
SmaI CCC'GGG
Cuts at: 181 2109 2238 181
Size: 1928 129 3159
Fragments arranged by size:
3159 1928 129
SmlI C'TyrA_G
Cuts at: 1056 2371 3286 4000 4262 1056
Size: 1315 915 714 262 2010
Fragments arranged by size:
2010 1315 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5216
SphI G_CATG'C
Cuts at: 2269 2769 2269
Size: 500 4716
StyI C'CwwG_G
Cuts at: 107 755 2350 2704 107
Size: 648 1595 354 2619
Fragments arranged by size:
2619 1595 648 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1636 2799 2932 3391 3600 1636
Size: 1163 133 459 209 3252
Fragments arranged by size:
3252 1163 459 209 133
TatI w'GTAC_w
Cuts at: 1745 4731 1745
Size: 2986 2230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1391 1423 1792 1824 4733 4765 1391
Size: 32 369 32 2909 32 1842
Fragments arranged by size:
2909 1842 369 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 898 3013 898
Size: 2115 3101
XbaI T'CTAG_A
Cuts at: 2247 2247
Size: 5216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 993 1048 1299 1551 1908 2711 993
Size: 55 251 252 357 803 3498
Fragments arranged by size:
3498 803 357 252 251 55
XhoI C'TCGA_G
Cuts at: 1056 1056
Size: 5216
XmaI C'CCGG_G
Cuts at: 179 2107 2236 179
Size: 1928 129 3159
Fragments arranged by size:
3159 1928 129
ZraI GAC'GTC
Cuts at: 324 324
Size: 5216
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AscI AvrII BaeI BfrBI BsiWI BsmI
BspEI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PfoI PmlI PsiI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI