Restriction map for
pF1KB3844
(Circular) (Six-base) MAPSORT of: pF1KB3844.seq Check: 2062 from: 1 to: 5135
pF1KB3844 5135 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 274 515 929 274
Size: 241 414 4480
Fragments arranged by size:
4480 414 241
AccI GT'mk_AC
Cuts at: 2173 2173
Size: 5135
Acc65I G'GTAC_C
Cuts at: 2151 2151
Size: 5135
AclI AA'CG_TT
Cuts at: 4975 4975
Size: 5135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 822 2960 3392 3745 4407 822
Size: 2138 432 353 662 1550
Fragments arranged by size:
2138 1550 662 432 353
AflIII A'CryG_T
Cuts at: 2487 4287 4568 2487
Size: 1800 281 3054
Fragments arranged by size:
3054 1800 281
AleI CACnn'nnGTG
Cuts at: 172 1674 172
Size: 1502 3633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 577 962 577
Size: 385 4750
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 196 228 196
Size: 32 5103
AlwNI CAG_nnn'CTG
Cuts at: 1077 1971 2045 2440 3878 1077
Size: 894 74 395 1438 2334
Fragments arranged by size:
2334 1438 894 395 74
ApaI G_GGCC'C
Cuts at: 1186 1186
Size: 5135
ApaLI G'TGCA_C
Cuts at: 1837 3973 1837
Size: 2136 2999
ApoI r'AATT_y
Cuts at: 2139 4488 4744 2139
Size: 2349 256 2530
Fragments arranged by size:
2530 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5135
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
AvaI C'yCGr_G
Cuts at: 687 1862 2155 687
Size: 1175 293 3667
Fragments arranged by size:
3667 1175 293
BamHI G'GATC_C
Cuts at: 2160 2160
Size: 5135
BanI G'GyrC_C
Cuts at: 782 1411 2074 2151 2565 2813 2848 782
Size: 629 663 77 414 248 35 3069
Fragments arranged by size:
3069 663 629 414 248 77 35
BanII G_rGCy'C
Cuts at: 230 866 1186 1543 1604 2149 3179 230
Size: 636 320 357 61 545 1030 2186
Fragments arranged by size:
2186 1030 636 545 357 320 61
BbeI G_GCGC'C
Cuts at: 786 1415 2569 2817 786
Size: 629 1154 248 3104
Fragments arranged by size:
3104 1154 629 248
BbsI GAAGACnn'nnnn_
Cuts at: 1791 1791
Size: 5135
BciVI GTATCCnnnnn_n'
Cuts at: 3033 4089 5135 3033
Size: 1056 1046 3033
Fragments arranged by size:
3033 1056 1046
BclI T'GATC_A
Cuts at: 2655 2655
Size: 5135
BglI GCCn_nnn'nGGC
Cuts at: 1276 1447 1276
Size: 171 4964
BglII A'GATC_T
Cuts at: 2650 4687 2650
Size: 2037 3098
BlpI GC'TnA_GC
Cuts at: 2247 2247
Size: 5135
Bme1580I G_kGCm'C
Cuts at: 1186 1841 2760 2853 3977 1186
Size: 655 919 93 1124 2344
Fragments arranged by size:
2344 1124 919 655 93
BmrI ACTGGGnnnn_n'
Cuts at: 1140 2347 2428 2763 4907 1140
Size: 1207 81 335 2144 1368
Fragments arranged by size:
2144 1368 1207 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3502 3502
Size: 5135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 727 1015 1618 586
Size: 141 288 603 4103
Fragments arranged by size:
4103 603 288 141
Bpu10I CC'TnA_GC
Cuts at: 658 673 1081 658
Size: 15 408 4712
Fragments arranged by size:
4712 408 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 628 1327 2311 3190 3904 4202 397
Size: 231 699 984 879 714 298 1330
Fragments arranged by size:
1330 984 879 714 699 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 1546 1633 4839 1546
Size: 87 3206 1842
Fragments arranged by size:
3206 1842 87
BsaAI yAC'GTr
Cuts at: 1434 1469 1656 1836 2007 2488 1434
Size: 35 187 180 171 481 4081
Fragments arranged by size:
4081 481 187 180 171 35
BsaBI GATnn'nnATC
Cuts at: 6 2649 4686 6
Size: 2643 2037 455
Fragments arranged by size:
2643 2037 455
BsaHI Gr'CG_yC
Cuts at: 353 620 783 1412 2566 2814 3516 5012 353
Size: 267 163 629 1154 248 702 1496 476
Fragments arranged by size:
1496 1154 702 629 476 267 248 163
BsaWI w'CCGG_w
Cuts at: 2324 2845 3523 3934 4081 4355 2324
Size: 521 678 411 147 274 3104
Fragments arranged by size:
3104 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 721 751 1591 1621 721
Size: 30 840 30 4235
Fragments arranged by size:
4235 840 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 628 724 1482 2014 628
Size: 96 758 532 3749
Fragments arranged by size:
3749 758 532 96
BseYI C'CCAG_C
Cuts at: 910 1070 1530 2560 3318 3983 4391 910
Size: 160 460 1030 758 665 408 1654
Fragments arranged by size:
1654 1030 758 665 460 408 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 487 559 1582 451
Size: 36 72 1023 4004
Fragments arranged by size:
4004 1023 72 36
BsiEI CG_ry'CG
Cuts at: 104 315 1151 2677 2723 3953 104
Size: 211 836 1526 46 1230 1286
Fragments arranged by size:
1526 1286 1230 836 211 46
BsiHKAI G_wGCw'C
Cuts at: 866 1841 2149 2927 3117 3977 866
Size: 975 308 778 190 860 2024
Fragments arranged by size:
2024 975 860 778 308 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1240 1240
Size: 5135
Bsp1286I G_dGCh'C
Cuts at: 230 866 1186 1543 1604 1841 2149 2760 2853
Size: 636 320 357 61 237 308 611 93
Cuts at: 2853 2927 3117 3179 3977 230
Size: 74 190 62 798 1388
Fragments arranged by size:
1388 798 636 611 357 320 308 237
190 93 74 62 61
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 5135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 274 377 423 488 515 812 914 929
Size: 68 103 46 65 27 297 102 15
Cuts at: 929 944 1592 2185 2399 2701 3082 206
Size: 15 648 593 214 302 381 2259
Fragments arranged by size:
2259 648 593 381 302 297 214 103
102 68 65 46 27 15 15
BsrBI CCG'CTC
Cuts at: 50 488 999 3427 3481 4965 5130 50
Size: 438 511 2428 54 1484 165 55
Fragments arranged by size:
2428 1484 511 438 165 55 54
BsrDI GCAATG_nn'
Cuts at: 530 1653 3047 530
Size: 1123 1394 2618
Fragments arranged by size:
2618 1394 1123
BsrFI r'CCGG_y
Cuts at: 3133 3314 3133
Size: 181 4954
BssSI C'ACGA_G
Cuts at: 3406 4114 3406
Size: 708 4427
BstAPI GCAn_nnn'nTGC
Cuts at: 266 1971 2045 2512 266
Size: 1705 74 467 2889
Fragments arranged by size:
2889 1705 467 74
BstBI TT'CG_AA
Cuts at: 3496 3496
Size: 5135
BstEII G'GTnAC_C
Cuts at: 799 1629 799
Size: 830 4305
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1230 1422 114
Size: 1116 192 3827
Fragments arranged by size:
3827 1116 192
BstYI r'GATC_y
Cuts at: 1 2160 2627 2650 2985 3231 3537 3549 3635
Size: 2159 467 23 335 246 306 12 86
Cuts at: 3635 4687 1
Size: 1052 449
Fragments arranged by size:
2159 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 182 191 1038 1151 1289 1394 1400 1535
Size: 75 9 847 113 138 105 6 135
Cuts at: 1535 1634 1748 1950 107
Size: 99 114 202 3292
Fragments arranged by size:
3292 847 202 138 135 114 113 105
99 75 9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 216 807 3085 3266 3429 216
Size: 591 2278 181 163 1922
Fragments arranged by size:
2278 1922 591 181 163
BtsI GCAGTG_nn'
Cuts at: 131 149 1793 2378 131
Size: 18 1644 585 2888
Fragments arranged by size:
2888 1644 585 18
DraI TTT'AAA
Cuts at: 2134 2134
Size: 5135
DrdI GACnn_nn'nnGTC
Cuts at: 2841 4185 2841
Size: 1344 3791
EaeI y'GGCC_r
Cuts at: 164 185 809 887 977 1148 1205 1268 1292
Size: 21 624 78 90 171 57 63 24
Cuts at: 1292 1397 1424 2720 2894 3285 3312 4657 164
Size: 105 27 1296 174 391 27 1345 642
Fragments arranged by size:
1345 1296 642 624 391 174 171 105
90 78 63 57 27 27 24 21
EagI C'GGCC_G
Cuts at: 1148 2720 1148
Size: 1572 3563
EarI CTCTTCn'nnn_
Cuts at: 658 1810 3158 3368 658
Size: 1152 1348 210 2425
Fragments arranged by size:
2425 1348 1152 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3343 4085 4231 4945 3343
Size: 742 146 714 3533
Fragments arranged by size:
3533 742 714 146
EcoICRI GAG'CTC
Cuts at: 864 2147 864
Size: 1283 3852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 727 822 1015 1618 2960 3392 3745 4407
Size: 141 95 193 603 1342 432 353 662
Cuts at: 4407 586
Size: 1314
Fragments arranged by size:
1342 1314 662 603 432 353 193 141
95
EcoNI CCTnn'n_nnAGG
Cuts at: 244 2047 244
Size: 1803 3332
EcoO109I rG'GnC_Cy
Cuts at: 408 765 1167 1182 1183 1440 1871 2274 408
Size: 357 402 15 1 257 431 403 3269
Fragments arranged by size:
3269 431 403 402 357 257 15 1
EcoRI G'AATT_C
Cuts at: 2139 2139
Size: 5135
EcoRV GAT'ATC
Cuts at: 342 342
Size: 5135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 669 3154 669
Size: 2485 2650
FspI TGC'GCA
Cuts at: 526 877 2916 526
Size: 351 2039 2745
Fragments arranged by size:
2745 2039 351
FspAI rTGC'GCAy
Cuts at: 526 526
Size: 5135
HaeII r_GCGC'y
Cuts at: 786 917 1415 2569 2817 4047 4818 786
Size: 131 498 1154 248 1230 771 1103
Fragments arranged by size:
1230 1154 1103 771 498 248 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 1591 1623 2977 3007 3009 3039 574
Size: 32 985 32 1354 30 2 30 2670
Fragments arranged by size:
2670 1354 985 32 32 30 30 2
HincII GTy'rAC
Cuts at: 426 1158 2174 426
Size: 732 1016 3387
Fragments arranged by size:
3387 1016 732
KasI G'GCGC_C
Cuts at: 782 1411 2565 2813 782
Size: 629 1154 248 3104
Fragments arranged by size:
3104 1154 629 248
KpnI G_GTAC'C
Cuts at: 2155 2155
Size: 5135
MluI A'CGCG_T
Cuts at: 4568 4568
Size: 5135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1037 1403 2054 3894 4078 1037
Size: 366 651 1840 184 2094
Fragments arranged by size:
2094 1840 651 366 184
MscI TGG'CCA
Cuts at: 811 979 1207 1270 1399 1426 2896 811
Size: 168 228 63 129 27 1470 3050
Fragments arranged by size:
3050 1470 228 168 129 63 27
MslI CAynn'nnrTG
Cuts at: 112 172 581 1432 1624 1674 3124 3251 112
Size: 60 409 851 192 50 1450 127 1996
Fragments arranged by size:
1996 1450 851 409 192 127 60 50
MspA1I CmG'CkG
Cuts at: 284 561 750 1040 1074 1952 2246 2560 2920
Size: 277 189 290 34 878 294 314 360
Cuts at: 2920 3704 3949 284
Size: 784 245 1470
Fragments arranged by size:
1470 878 784 360 314 294 290 277
245 189 34
NaeI GCC'GGC
Cuts at: 3316 3316
Size: 5135
NarI GG'CG_CC
Cuts at: 783 1412 2566 2814 783
Size: 629 1154 248 3104
Fragments arranged by size:
3104 1154 629 248
NcoI C'CATG_G
Cuts at: 107 191 1394 1634 1748 107
Size: 84 1203 240 114 3494
Fragments arranged by size:
3494 1203 240 114 84
NgoMIV G'CCGG_C
Cuts at: 3314 3314
Size: 5135
NspI r_CATG'y
Cuts at: 757 2188 2688 4291 757
Size: 1431 500 1603 1601
Fragments arranged by size:
1603 1601 1431 500
PasI CC'CwG_GG
Cuts at: 1323 1957 1323
Size: 634 4501
PciI A'CATG_T
Cuts at: 4287 4287
Size: 5135
PflMI CCAn_nnn'nTGG
Cuts at: 1829 1829
Size: 5135
PfoI T'CCnGG_A
Cuts at: 494 1940 494
Size: 1446 3689
PmeI GTTT'AAAC
Cuts at: 2134 2134
Size: 5135
PmlI CAC'GTG
Cuts at: 1434 1469 1434
Size: 35 5100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 5103
PpuMI rG'GwC_Cy
Cuts at: 408 408
Size: 5135
PshAI GACnn'nnGTC
Cuts at: 247 421 247
Size: 174 4961
PspOMI G'GGCC_C
Cuts at: 1182 1182
Size: 5135
PstI C_TGCA'G
Cuts at: 374 512 926 941 1967 2182 2867 374
Size: 138 414 15 1026 215 685 2642
Fragments arranged by size:
2642 1026 685 414 215 138 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5135
PvuII CAG'CTG
Cuts at: 1074 2560 2920 1074
Size: 1486 360 3289
Fragments arranged by size:
3289 1486 360
RsrII CG'GwC_CG
Cuts at: 3330 3330
Size: 5135
SacI G_AGCT'C
Cuts at: 866 2149 866
Size: 1283 3852
SacII CC_GC'GG
Cuts at: 1041 1953 1041
Size: 912 4223
SalI G'TCGA_C
Cuts at: 2172 2172
Size: 5135
SapI GCTCTTCn'nnn_
Cuts at: 658 3158 3368 658
Size: 2500 210 2425
Fragments arranged by size:
2500 2425 210
SbfI CC_TGCA'GG
Cuts at: 512 941 2182 512
Size: 429 1241 3465
Fragments arranged by size:
3465 1241 429
ScaI AGT'ACT
Cuts at: 4652 4652
Size: 5135
SfcI C'TryA_G
Cuts at: 33 370 508 847 922 937 1963 2178 2863
Size: 337 138 339 75 15 1026 215 685
Cuts at: 2863 3831 4022 33
Size: 968 191 1146
Fragments arranged by size:
1146 1026 968 685 339 337 215 191
138 75 15
SfoI GGC'GCC
Cuts at: 784 1413 2567 2815 784
Size: 629 1154 248 3104
Fragments arranged by size:
3104 1154 629 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
SmaI CCC'GGG
Cuts at: 1864 2157 1864
Size: 293 4842
SmlI C'TyrA_G
Cuts at: 412 643 1342 2290 3205 3919 4181 412
Size: 231 699 948 915 714 262 1366
Fragments arranged by size:
1366 948 915 714 699 262 231
SnaBI TAC'GTA
Cuts at: 1656 1656
Size: 5135
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5135
SphI G_CATG'C
Cuts at: 757 2188 2688 757
Size: 1431 500 3204
Fragments arranged by size:
3204 1431 500
StyI C'CwwG_G
Cuts at: 107 191 419 1093 1394 1556 1634 1682 1748
Size: 84 228 674 301 162 78 48 66
Cuts at: 1748 2269 2623 107
Size: 521 354 2619
Fragments arranged by size:
2619 674 521 354 301 228 162 84
78 66 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 2718 2851 3310 3519 1122
Size: 1596 133 459 209 2738
Fragments arranged by size:
2738 1596 459 209 133
TatI w'GTAC_w
Cuts at: 4650 4650
Size: 5135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4652 4684 4652
Size: 32 5103
Tth111I GACn'n_nGTC
Cuts at: 2932 2932
Size: 5135
XbaI T'CTAG_A
Cuts at: 2166 2166
Size: 5135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 543 2630 189
Size: 354 2087 2694
Fragments arranged by size:
2694 2087 354
XmaI C'CCGG_G
Cuts at: 1862 2155 1862
Size: 293 4842
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI