Restriction map for
pF1KB3856
(Circular) (Six-base) MAPSORT of: pF1KB3856.seq Check: 498 from: 1 to: 6836
pF1KB3856 6836 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 380 380
Size: 6836
AccI GT'mk_AC
Cuts at: 3874 3874
Size: 6836
Acc65I G'GTAC_C
Cuts at: 3852 3852
Size: 6836
AclI AA'CG_TT
Cuts at: 6676 6676
Size: 6836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1934 2138 4661 5093 5840 6108 796
Size: 1138 204 2523 432 747 268 1524
Fragments arranged by size:
2523 1524 1138 747 432 268 204
AfeI AGC'GCT
Cuts at: 1183 2351 1183
Size: 1168 5668
AflIII A'CryG_T
Cuts at: 4188 5292 6269 4188
Size: 1104 977 4755
Fragments arranged by size:
4755 1104 977
AgeI A'CCGG_T
Cuts at: 935 5224 935
Size: 4289 2547
AleI CACnn'nnGTG
Cuts at: 1397 1397
Size: 6836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1954 3730 1954
Size: 1776 5060
AlwNI CAG_nnn'CTG
Cuts at: 858 900 1287 2050 2232 2316 2391 2991 4141
Size: 42 387 763 182 84 75 600 1150
Cuts at: 4141 5708 858
Size: 1567 1986
Fragments arranged by size:
1986 1567 1150 763 600 387 182 84
75 42
ApaI G_GGCC'C
Cuts at: 181 412 519 678 1044 1056 1792 2417 181
Size: 231 107 159 366 12 736 625 4600
Fragments arranged by size:
4600 736 625 366 231 159 107 12
ApaLI G'TGCA_C
Cuts at: 5606 5606
Size: 6836
ApoI r'AATT_y
Cuts at: 1150 2866 3467 3840 6189 6445 1150
Size: 1716 601 373 2349 256 1541
Fragments arranged by size:
2349 1716 1541 601 373 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6836
AvaI C'yCGr_G
Cuts at: 199 435 518 659 762 866 1037 1049 1791
Size: 236 83 141 103 104 171 12 742
Cuts at: 1791 2789 3856 199
Size: 998 1067 3179
Fragments arranged by size:
3179 1067 998 742 236 171 141 104
103 83 12
BamHI G'GATC_C
Cuts at: 2897 3503 3861 2897
Size: 606 358 5872
Fragments arranged by size:
5872 606 358
BanI G'GyrC_C
Cuts at: 125 431 757 767 794 1752 1758 2177 2380
Size: 306 326 10 27 958 6 419 203
Cuts at: 2380 2836 2925 3333 3852 4266 4514 4549 125
Size: 456 89 408 519 414 248 35 2412
Fragments arranged by size:
2412 958 519 456 419 414 408 326
306 248 203 89 35 27 10 6
BanII G_rGCy'C
Cuts at: 181 412 519 525 678 1044 1056 1121 1235
Size: 231 107 6 153 366 12 65 114
Cuts at: 1235 1374 1453 1792 2063 2417 3070 3196 3850
Size: 139 79 339 271 354 653 126 654
Cuts at: 3850 4880 181
Size: 1030 2137
Fragments arranged by size:
2137 1030 654 653 366 354 339 271
231 153 139 126 114 107 79 65
12 6
BbeI G_GCGC'C
Cuts at: 129 435 771 2181 4270 4518 129
Size: 306 336 1410 2089 248 2447
Fragments arranged by size:
2447 2089 1410 336 306 248
BbsI GAAGACnn'nnnn_
Cuts at: 218 320 2311 2358 3111 218
Size: 102 1991 47 753 3943
Fragments arranged by size:
3943 1991 753 102 47
BbvCI CC'TCA_GC
Cuts at: 509 509
Size: 6836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 739 773 739
Size: 34 6802
BciVI GTATCCnnnnn_n'
Cuts at: 4734 5495 6836 4734
Size: 761 1341 4734
Fragments arranged by size:
4734 1341 761
BclI T'GATC_A
Cuts at: 4356 4356
Size: 6836
BglI GCCn_nnn'nGGC
Cuts at: 429 505 1125 1255 1879 3667 429
Size: 76 620 130 624 1788 3598
Fragments arranged by size:
3598 1788 624 620 130 76
BglII A'GATC_T
Cuts at: 2582 2642 4351 6388 2582
Size: 60 1709 2037 3030
Fragments arranged by size:
3030 2037 1709 60
BlpI GC'TnA_GC
Cuts at: 848 2673 2992 3948 848
Size: 1825 319 956 3736
Fragments arranged by size:
3736 1825 956 319
Bme1580I G_kGCm'C
Cuts at: 181 412 519 678 797 1044 1056 1792 2385
Size: 231 107 159 119 247 12 736 593
Cuts at: 2385 2417 2930 3249 4461 4554 5610 181
Size: 32 513 319 1212 93 1056 1407
Fragments arranged by size:
1407 1212 1056 736 593 513 319 247
231 159 119 107 93 32 12
BmgBI CAC'GTC
Cuts at: 1164 1500 1164
Size: 336 6500
BmrI ACTGGGnnnn_n'
Cuts at: 851 1116 2996 4048 4129 4464 6608 851
Size: 265 1880 1052 81 335 2144 1079
Fragments arranged by size:
2144 1880 1079 1052 335 265 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1704 5203 1704
Size: 3499 3337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 1249 1556 1561 1648 2077 2338 2488 2557
Size: 776 307 5 87 429 261 150 69
Cuts at: 2557 473
Size: 4752
Fragments arranged by size:
4752 776 429 307 261 150 87 69
5
Bpu10I CC'TnA_GC
Cuts at: 509 509
Size: 6836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1993 4012 4891 5383 5681 1993
Size: 2019 879 492 298 3148
Fragments arranged by size:
3148 2019 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 278 1525 2614 6540 278
Size: 1247 1089 3926 574
Fragments arranged by size:
3926 1247 1089 574
BsaAI yAC'GTr
Cuts at: 1200 4189 1200
Size: 2989 3847
BsaBI GATnn'nnATC
Cuts at: 6 1324 4350 6387 6
Size: 1318 3026 2037 455
Fragments arranged by size:
3026 2037 1318 455
BsaHI Gr'CG_yC
Cuts at: 126 187 432 768 2178 4267 4515 5217 6713
Size: 61 245 336 1410 2089 248 702 1496
Cuts at: 6713 126
Size: 249
Fragments arranged by size:
2089 1496 1410 702 336 249 248 245
61
BsaWI w'CCGG_w
Cuts at: 378 596 935 3581 4025 4546 5224 5498 5645
Size: 218 339 2646 444 521 678 274 147
Cuts at: 5645 6056 378
Size: 411 1158
Fragments arranged by size:
2646 1158 678 521 444 411 339 274
218 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 256 286 1108 1138 1189 1219 256
Size: 30 822 30 51 30 5873
Fragments arranged by size:
5873 822 51 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 301 487 546 647 712 834 1183 1186
Size: 36 186 59 101 65 122 349 3
Cuts at: 1186 1981 2035 2446 265
Size: 795 54 411 4655
Fragments arranged by size:
4655 795 411 349 186 122 101 65
59 54 36 3
BseYI C'CCAG_C
Cuts at: 785 1184 1458 1785 2050 2295 2376 2384 2739
Size: 399 274 327 265 245 81 8 355
Cuts at: 2739 2764 3038 4261 5019 5596 6092 785
Size: 25 274 1223 758 577 496 1529
Fragments arranged by size:
1529 1223 758 577 496 399 355 327
274 274 265 245 81 25 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 614 1978 583
Size: 31 1364 5441
Fragments arranged by size:
5441 1364 31
BsiEI CG_ry'CG
Cuts at: 104 911 1827 3738 4378 4424 5632 104
Size: 807 916 1911 640 46 1208 1308
Fragments arranged by size:
1911 1308 1208 916 807 640 46
BsiHKAI G_wGCw'C
Cuts at: 1235 1265 2657 2828 3461 3719 3850 4628 4818
Size: 30 1392 171 633 258 131 778 190
Cuts at: 4818 5610 1235
Size: 792 2461
Fragments arranged by size:
2461 1392 792 778 633 258 190 171
131 30
BsmI GAATG_Cn'
Cuts at: 987 1650 987
Size: 663 6173
BsmBI CGTCTCn'nnnn_
Cuts at: 2568 2568
Size: 6836
Bsp1286I G_dGCh'C
Cuts at: 181 412 519 525 678 797 1044 1056 1121
Size: 231 107 6 153 119 247 12 65
Cuts at: 1121 1235 1265 1374 1453 1792 2063 2385 2417
Size: 114 30 109 79 339 271 322 32
Cuts at: 2417 2657 2828 2930 3070 3196 3249 3461 3719
Size: 240 171 102 140 126 53 212 258
Cuts at: 3719 3850 4461 4554 4628 4818 4880 5610 181
Size: 131 611 93 74 190 62 730 1407
Fragments arranged by size:
1407 730 611 339 322 271 258 247
240 231 212 190 171 153 140 131
126 119 114 109 107 102 93 79
74 65 62 53 32 30 12 6
BspEI T'CCGG_A
Cuts at: 378 3581 6056 378
Size: 3203 2475 1158
Fragments arranged by size:
3203 2475 1158
BspHI T'CATG_A
Cuts at: 923 3317 6012 923
Size: 2394 2695 1747
Fragments arranged by size:
2695 2394 1747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 1907 3221 3886 4100 4402 4783 380
Size: 1527 1314 665 214 302 381 2433
Fragments arranged by size:
2433 1527 1314 665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 243 1598 1829 1890 3287 3519 3702 3789
Size: 193 1355 231 61 1397 232 183 87
Cuts at: 3789 5128 5182 6666 6831 50
Size: 1339 54 1484 165 55
Fragments arranged by size:
1484 1397 1355 1339 232 231 193 183
165 87 61 55 54
BsrDI GCAATG_nn'
Cuts at: 4748 4748
Size: 6836
BsrFI r'CCGG_y
Cuts at: 935 1755 2030 2833 2879 3774 4834 5015 5224
Size: 820 275 803 46 895 1060 181 209
Cuts at: 5224 935
Size: 2547
Fragments arranged by size:
2547 1060 895 820 803 275 209 181
46
BsrGI T'GTAC_A
Cuts at: 3476 3476
Size: 6836
BssSI C'ACGA_G
Cuts at: 275 1612 1816 1864 5107 5465 275
Size: 1337 204 48 3243 358 1646
Fragments arranged by size:
3243 1646 1337 358 204 48
BstAPI GCAn_nnn'nTGC
Cuts at: 858 972 1287 2661 3531 4213 858
Size: 114 315 1374 870 682 3481
Fragments arranged by size:
3481 1374 870 682 315 114
BstBI TT'CG_AA
Cuts at: 5197 5197
Size: 6836
BstXI CCAn_nnnn'nTGG
Cuts at: 3828 3828
Size: 6836
BstYI r'GATC_y
Cuts at: 1 254 1269 2582 2642 2897 3429 3443 3503
Size: 253 1015 1313 60 255 532 14 60
Cuts at: 3503 3861 4328 4351 4686 4932 5944 6030 6042
Size: 358 467 23 335 246 1012 86 12
Cuts at: 6042 6388 1
Size: 346 449
Fragments arranged by size:
1313 1015 1012 532 467 449 358 346
335 255 253 246 86 60 60 23
14 12
BtgI C'CryG_G
Cuts at: 875 905 1251 1470 1655 1700 3230 875
Size: 30 346 219 185 45 1530 4481
Fragments arranged by size:
4481 1530 346 219 185 45 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2832 2916 4786 4967 5130 2832
Size: 84 1870 181 163 4538
Fragments arranged by size:
4538 1870 181 163 84
BtsI GCAGTG_nn'
Cuts at: 634 734 856 1748 4079 634
Size: 100 122 892 2331 3391
Fragments arranged by size:
3391 2331 892 122 100
DraI TTT'AAA
Cuts at: 3835 3835
Size: 6836
DraIII CAC_nnn'GTG
Cuts at: 972 1003 1308 972
Size: 31 305 6500
Fragments arranged by size:
6500 305 31
DrdI GACnn_nn'nnGTC
Cuts at: 1498 4542 5400 1498
Size: 3044 858 2934
Fragments arranged by size:
3044 2934 858
EaeI y'GGCC_r
Cuts at: 725 902 908 1005 1824 1907 2234 2831 2892
Size: 177 6 97 819 83 327 597 61
Cuts at: 2892 3227 3452 4421 4595 4986 5013 6358 725
Size: 335 225 969 174 391 27 1345 1203
Fragments arranged by size:
1345 1203 969 819 597 391 335 327
225 177 174 97 83 61 27 6
EagI C'GGCC_G
Cuts at: 908 1824 4421 908
Size: 916 2597 3323
Fragments arranged by size:
3323 2597 916
EarI CTCTTCn'nnn_
Cuts at: 922 1151 1780 3058 4859 5069 922
Size: 229 629 1278 1801 210 2689
Fragments arranged by size:
2689 1801 1278 629 229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 718 724 2151 5044 5354 5500 6646 718
Size: 6 1427 2893 310 146 1146 908
Fragments arranged by size:
2893 1427 1146 908 310 146 6
EcoICRI GAG'CTC
Cuts at: 1233 3848 1233
Size: 2615 4221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 796 1249 1556 1561 1648 1934 2077 2138
Size: 323 453 307 5 87 286 143 61
Cuts at: 2138 2338 2488 2557 4661 5093 5840 6108 473
Size: 200 150 69 2104 432 747 268 1201
Fragments arranged by size:
2104 1201 747 453 432 323 307 286
268 200 150 143 87 69 61 5
EcoNI CCTnn'n_nnAGG
Cuts at: 262 262
Size: 6836
EcoO109I rG'GnC_Cy
Cuts at: 178 408 515 674 870 1053 1241 1896 2313
Size: 230 107 159 196 183 188 655 417
Cuts at: 2313 2413 2414 3975 178
Size: 100 1 1561 3039
Fragments arranged by size:
3039 1561 655 417 230 196 188 183
159 107 100 1
EcoRI G'AATT_C
Cuts at: 3840 3840
Size: 6836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 235 3087 4855 235
Size: 2852 1768 2216
Fragments arranged by size:
2852 2216 1768
FspI TGC'GCA
Cuts at: 2863 4617 2863
Size: 1754 5082
HaeII r_GCGC'y
Cuts at: 119 129 435 771 1185 2001 2086 2181 2353
Size: 10 306 336 414 816 85 95 172
Cuts at: 2353 4270 4518 5540 6519 119
Size: 1917 248 1022 979 436
Fragments arranged by size:
1917 1022 979 816 436 414 336 306
248 172 95 85 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 600 632 2050 2082 3735 3767 4678
Size: 32 312 32 1418 32 1653 32 911
Cuts at: 4678 4708 4710 4740 256
Size: 30 2 30 2352
Fragments arranged by size:
2352 1653 1418 911 312 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3875 3875
Size: 6836
KasI G'GCGC_C
Cuts at: 125 431 767 2177 4266 4514 125
Size: 306 336 1410 2089 248 2447
Fragments arranged by size:
2447 2089 1410 336 306 248
KpnI G_GTAC'C
Cuts at: 3856 3856
Size: 6836
MluI A'CGCG_T
Cuts at: 6269 6269
Size: 6836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1715 1727 2309 2705 5507 5691 1715
Size: 12 582 396 2802 184 2860
Fragments arranged by size:
2860 2802 582 396 184 12
MscI TGG'CCA
Cuts at: 727 904 3229 4597 727
Size: 177 2325 1368 2966
Fragments arranged by size:
2966 2325 1368 177
MslI CAynn'nnrTG
Cuts at: 1397 1469 1843 2947 3133 3316 3557 3712 3826
Size: 72 374 1104 186 183 241 155 114
Cuts at: 3826 4825 4952 1397
Size: 999 127 3281
Fragments arranged by size:
3281 1104 999 374 241 186 183 155
127 114 72
MspA1I CmG'CkG
Cuts at: 1438 1585 2116 3375 3618 3658 3947 4261 4621
Size: 147 531 1259 243 40 289 314 360
Cuts at: 4621 5634 5879 1438
Size: 1013 245 2395
Fragments arranged by size:
2395 1259 1013 531 360 314 289 245
243 147 40
NaeI GCC'GGC
Cuts at: 2032 2835 5017 2032
Size: 803 2182 3851
Fragments arranged by size:
3851 2182 803
NarI GG'CG_CC
Cuts at: 126 432 768 2178 4267 4515 126
Size: 306 336 1410 2089 248 2447
Fragments arranged by size:
2447 2089 1410 336 306 248
NcoI C'CATG_G
Cuts at: 1251 1470 1700 3230 1251
Size: 219 230 1530 4857
Fragments arranged by size:
4857 1530 230 219
NgoMIV G'CCGG_C
Cuts at: 2030 2833 5015 2030
Size: 803 2182 3851
Fragments arranged by size:
3851 2182 803
NspI r_CATG'y
Cuts at: 3183 3889 4389 5296 3183
Size: 706 500 907 4723
Fragments arranged by size:
4723 907 706 500
PasI CC'CwG_GG
Cuts at: 181 231 405 1446 3054 181
Size: 50 174 1041 1608 3963
Fragments arranged by size:
3963 1608 1041 174 50
PciI A'CATG_T
Cuts at: 5292 5292
Size: 6836
PflMI CCAn_nnn'nTGG
Cuts at: 900 1308 1311 1585 2361 900
Size: 408 3 274 776 5375
Fragments arranged by size:
5375 776 408 274 3
PfoI T'CCnGG_A
Cuts at: 1100 1226 1718 1922 2648 1100
Size: 126 492 204 726 5288
Fragments arranged by size:
5288 726 492 204 126
PmeI GTTT'AAAC
Cuts at: 3835 3835
Size: 6836
PmlI CAC'GTG
Cuts at: 1200 1200
Size: 6836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5975 6007 5975
Size: 32 6804
PpuMI rG'GwC_Cy
Cuts at: 870 1241 1896 2313 870
Size: 371 655 417 5393
Fragments arranged by size:
5393 655 417 371
PspOMI G'GGCC_C
Cuts at: 177 408 515 674 1040 1052 1788 2413 177
Size: 231 107 159 366 12 736 625 4600
Fragments arranged by size:
4600 736 625 366 231 159 107 12
PstI C_TGCA'G
Cuts at: 741 1933 2093 3188 3617 3883 4568 741
Size: 1192 160 1095 429 266 685 3009
Fragments arranged by size:
3009 1192 1095 685 429 266 160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6836
PvuII CAG'CTG
Cuts at: 1438 1585 2116 3618 3658 4261 4621 1438
Size: 147 531 1502 40 603 360 3653
Fragments arranged by size:
3653 1502 603 531 360 147 40
RsrII CG'GwC_CG
Cuts at: 5031 5031
Size: 6836
SacI G_AGCT'C
Cuts at: 1235 3850 1235
Size: 2615 4221
SalI G'TCGA_C
Cuts at: 3873 3873
Size: 6836
SanDI GG'GwC_CC
Cuts at: 870 870
Size: 6836
SapI GCTCTTCn'nnn_
Cuts at: 3058 4859 5069 3058
Size: 1801 210 4825
Fragments arranged by size:
4825 1801 210
SbfI CC_TGCA'GG
Cuts at: 3883 3883
Size: 6836
ScaI AGT'ACT
Cuts at: 6353 6353
Size: 6836
SexAI A'CCwGG_T
Cuts at: 1304 2474 1304
Size: 1170 5666
SfcI C'TryA_G
Cuts at: 33 259 737 1929 2042 2089 2451 3184 3613
Size: 226 478 1192 113 47 362 733 429
Cuts at: 3613 3879 4564 5557 5748 33
Size: 266 685 993 191 1121
Fragments arranged by size:
1192 1121 993 733 685 478 429 362
266 226 191 113 47
SfoI GGC'GCC
Cuts at: 127 433 769 2179 4268 4516 127
Size: 306 336 1410 2089 248 2447
Fragments arranged by size:
2447 2089 1410 336 306 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6836
SmaI CCC'GGG
Cuts at: 437 520 868 1051 2791 3858 437
Size: 83 348 183 1740 1067 3415
Fragments arranged by size:
3415 1740 1067 348 183 83
SmlI C'TyrA_G
Cuts at: 2008 3991 4906 5398 5660 2008
Size: 1983 915 492 262 3184
Fragments arranged by size:
3184 1983 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6836
SphI G_CATG'C
Cuts at: 3183 3889 4389 3183
Size: 706 500 5630
Fragments arranged by size:
5630 706 500
SrfI GCCC'GGGC
Cuts at: 520 520
Size: 6836
StuI AGG'CCT
Cuts at: 2437 2437
Size: 6836
StyI C'CwwG_G
Cuts at: 134 1251 1470 1700 3230 3970 4324 134
Size: 1117 219 230 1530 740 354 2646
Fragments arranged by size:
2646 1530 1117 740 354 230 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 371 382 1409 2368 2590 3569 3752 4419 4552
Size: 11 1027 959 222 979 183 667 133
Cuts at: 4552 5011 5220 371
Size: 459 209 1987
Fragments arranged by size:
1987 1027 979 959 667 459 222 209
183 133 11
TatI w'GTAC_w
Cuts at: 3476 3806 6351 3476
Size: 330 2545 3961
Fragments arranged by size:
3961 2545 330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 319 346 351 378 677 709 1108 1140 1189
Size: 27 5 27 299 32 399 32 49
Cuts at: 1189 1221 2999 3031 6353 6385 319
Size: 32 1778 32 3322 32 770
Fragments arranged by size:
3322 1778 770 399 299 49 32 32
32 32 32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 4633 4633
Size: 6836
XbaI T'CTAG_A
Cuts at: 3867 3867
Size: 6836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2943 4331 2943
Size: 1388 5448
XmaI C'CCGG_G
Cuts at: 435 518 866 1049 2789 3856 435
Size: 83 348 183 1740 1067 3415
Fragments arranged by size:
3415 1740 1067 348 183 83
XmnI GAAnn'nnTTC
Cuts at: 1411 2644 1411
Size: 1233 5603
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SfiI SgrAI SnaBI SspI SwaI XhoI ZraI