Restriction map for
pF1KB3865
(Circular) (Six-base) MAPSORT of: pF1KB3865.seq Check: 5809 from: 1 to: 4115
pF1KB3865 4115 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 545 545
Size: 4115
AccI GT'mk_AC
Cuts at: 680 1153 680
Size: 473 3642
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2372 3119 3387 1940
Size: 432 747 268 2668
Fragments arranged by size:
2668 747 432 268
AflIII A'CryG_T
Cuts at: 320 1467 2571 3548 320
Size: 1147 1104 977 887
Fragments arranged by size:
1147 1104 977 887
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1032 1032
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 1420 2987 1420
Size: 1567 2548
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 1119 3468 3724 1119
Size: 2349 256 1510
Fragments arranged by size:
2349 1510 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 1135 1135
Size: 4115
BamHI G'GATC_C
Cuts at: 1140 1140
Size: 4115
BanI G'GyrC_C
Cuts at: 179 256 604 1131 1545 1793 1828 179
Size: 77 348 527 414 248 35 2466
Fragments arranged by size:
2466 527 414 348 248 77 35
BanII G_rGCy'C
Cuts at: 719 1004 1129 2159 719
Size: 285 125 1030 2675
Fragments arranged by size:
2675 1030 285 125
BbeI G_GCGC'C
Cuts at: 260 1549 1797 260
Size: 1289 248 2578
Fragments arranged by size:
2578 1289 248
BbsI GAAGACnn'nnnn_
Cuts at: 838 838
Size: 4115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4081
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglI GCCn_nnn'nGGC
Cuts at: 671 671
Size: 4115
BglII A'GATC_T
Cuts at: 1630 3667 1630
Size: 2037 2078
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 446 607 1740 1833 2889 446
Size: 161 1133 93 1056 1672
Fragments arranged by size:
1672 1133 1056 161 93
BmgBI CAC'GTC
Cuts at: 281 281
Size: 4115
BmrI ACTGGGnnnn_n'
Cuts at: 1327 1408 1743 3887 1327
Size: 81 335 2144 1555
Fragments arranged by size:
2144 1555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 645 733 1018 645
Size: 88 285 3742
Fragments arranged by size:
3742 285 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 2170 2662 2960 1291
Size: 879 492 298 2446
Fragments arranged by size:
2446 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 637 3819 637
Size: 3182 933
BsaAI yAC'GTr
Cuts at: 321 369 441 543 1468 321
Size: 48 72 102 925 2968
Fragments arranged by size:
2968 925 102 72 48
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 257 376 487 550 578 1546 1794 2496 3992
Size: 119 111 63 28 968 248 702 1496
Cuts at: 3992 257
Size: 380
Fragments arranged by size:
1496 968 702 380 248 119 111 63
28
BsaWI w'CCGG_w
Cuts at: 1304 1825 2503 2777 2924 3335 1304
Size: 521 678 274 147 411 2084
Fragments arranged by size:
2084 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1540 2298 2875 3371 1540
Size: 758 577 496 2284
Fragments arranged by size:
2284 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 418 421 523 1002 261
Size: 157 3 102 479 3374
Fragments arranged by size:
3374 479 157 102 3
BsiEI CG_ry'CG
Cuts at: 104 137 1657 1703 2911 104
Size: 33 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 190 719 1129 1907 2097 2889 190
Size: 529 410 778 190 792 1416
Fragments arranged by size:
1416 792 778 529 410 190
BsmBI CGTCTCn'nnnn_
Cuts at: 111 541 569 111
Size: 430 28 3657
Fragments arranged by size:
3657 430 28
Bsp1286I G_dGCh'C
Cuts at: 190 446 607 719 1004 1129 1740 1833 1907
Size: 256 161 112 285 125 611 93 74
Cuts at: 1907 2097 2159 2889 190
Size: 190 62 730 1416
Fragments arranged by size:
1416 730 611 285 256 190 161 125
112 93 74 62
BspEI T'CCGG_A
Cuts at: 3335 3335
Size: 4115
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 899 1165 1379 1681 2062 545
Size: 354 266 214 302 381 2598
Fragments arranged by size:
2598 381 354 302 266 214
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2027 2027
Size: 4115
BsrFI r'CCGG_y
Cuts at: 517 851 2113 2294 2503 517
Size: 334 1262 181 209 2129
Fragments arranged by size:
2129 1262 334 209 181
BsrGI T'GTAC_A
Cuts at: 323 323
Size: 4115
BssHII G'CGCG_C
Cuts at: 499 499
Size: 4115
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 1492 1492
Size: 4115
BstBI TT'CG_AA
Cuts at: 722 2476 722
Size: 1754 2361
BstXI CCAn_nnnn'nTGG
Cuts at: 369 1029 369
Size: 660 3455
BstYI r'GATC_y
Cuts at: 1 1025 1140 1607 1630 1965 2211 3223 3309
Size: 1024 115 467 23 335 246 1012 86
Cuts at: 3309 3321 3667 1
Size: 12 346 449
Fragments arranged by size:
1024 1012 467 449 346 335 246 115
86 23 12
BtgI C'CryG_G
Cuts at: 340 598 796 340
Size: 258 198 3659
Fragments arranged by size:
3659 258 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2065 2246 2409 2065
Size: 181 163 3771
Fragments arranged by size:
3771 181 163
BtsI GCAGTG_nn'
Cuts at: 234 1358 234
Size: 1124 2991
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DraIII CAC_nnn'GTG
Cuts at: 372 372
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 2679 1821
Size: 858 3257
EaeI y'GGCC_r
Cuts at: 422 853 1700 1874 2265 2292 3637 422
Size: 431 847 174 391 27 1345 900
Fragments arranged by size:
1345 900 847 431 391 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 109 719 724 2138 2348 109
Size: 610 5 1414 210 1876
Fragments arranged by size:
1876 1414 610 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 2633 2779 3925 2323
Size: 310 146 1146 2513
Fragments arranged by size:
2513 1146 310 146
EcoICRI GAG'CTC
Cuts at: 717 1127 717
Size: 410 3705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 645 733 1018 1940 2372 3119 3387 645
Size: 88 285 922 432 747 268 1373
Fragments arranged by size:
1373 922 747 432 285 268 88
EcoO109I rG'GnC_Cy
Cuts at: 792 1254 792
Size: 462 3653
EcoRI G'AATT_C
Cuts at: 1119 1119
Size: 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1101 2134 1101
Size: 1033 3082
FspI TGC'GCA
Cuts at: 310 733 1896 310
Size: 423 1163 2529
Fragments arranged by size:
2529 1163 423
FspAI rTGC'GCAy
Cuts at: 310 310
Size: 4115
HaeII r_GCGC'y
Cuts at: 260 1549 1797 2819 3798 260
Size: 1289 248 1022 979 577
Fragments arranged by size:
1289 1022 979 577 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 110 142 869 901 965 997 1957 1987 1989
Size: 32 727 32 64 32 960 30 2
Cuts at: 1989 2019 110
Size: 30 2206
Fragments arranged by size:
2206 960 727 64 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 888 1154 888
Size: 266 3849
KasI G'GCGC_C
Cuts at: 256 1545 1793 256
Size: 1289 248 2578
Fragments arranged by size:
2578 1289 248
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 2786 2970 371
Size: 2415 184 1516
Fragments arranged by size:
2415 1516 184
MscI TGG'CCA
Cuts at: 424 1876 424
Size: 1452 2663
MslI CAynn'nnrTG
Cuts at: 218 367 2104 2231 218
Size: 149 1737 127 2102
Fragments arranged by size:
2102 1737 149 127
MspA1I CmG'CkG
Cuts at: 492 600 1226 1540 1900 2913 3158 492
Size: 108 626 314 360 1013 245 1449
Fragments arranged by size:
1449 1013 626 360 314 245 108
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 4115
NarI GG'CG_CC
Cuts at: 257 1546 1794 257
Size: 1289 248 2578
Fragments arranged by size:
2578 1289 248
NcoI C'CATG_G
Cuts at: 340 340
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 4115
NspI r_CATG'y
Cuts at: 1168 1668 2575 1168
Size: 500 907 2708
Fragments arranged by size:
2708 907 500
PasI CC'CwG_GG
Cuts at: 266 266
Size: 4115
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PflMI CCAn_nnn'nTGG
Cuts at: 459 459
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PmlI CAC'GTG
Cuts at: 369 441 543 369
Size: 72 102 3941
Fragments arranged by size:
3941 102 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PstI C_TGCA'G
Cuts at: 908 1162 1847 908
Size: 254 685 3176
Fragments arranged by size:
3176 685 254
PvuI CG_AT'CG
Cuts at: 104 137 104
Size: 33 4082
PvuII CAG'CTG
Cuts at: 1540 1900 1540
Size: 360 3755
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 719 1129 719
Size: 410 3705
SacII CC_GC'GG
Cuts at: 601 601
Size: 4115
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 724 2138 2348 724
Size: 1414 210 2491
Fragments arranged by size:
2491 1414 210
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 274 3632 274
Size: 3358 757
SfcI C'TryA_G
Cuts at: 33 384 705 904 1158 1843 2836 3027 33
Size: 351 321 199 254 685 993 191 1121
Fragments arranged by size:
1121 993 685 351 321 254 199 191
SfoI GGC'GCC
Cuts at: 258 1547 1795 258
Size: 1289 248 2578
Fragments arranged by size:
2578 1289 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 1137 1137
Size: 4115
SmlI C'TyrA_G
Cuts at: 1270 2185 2677 2939 1270
Size: 915 492 262 2446
Fragments arranged by size:
2446 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 1168 1668 1168
Size: 500 3615
StuI AGG'CCT
Cuts at: 919 919
Size: 4115
StyI C'CwwG_G
Cuts at: 340 1004 1249 1603 340
Size: 664 245 354 2852
Fragments arranged by size:
2852 664 354 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 763 1698 1831 2290 2499 763
Size: 935 133 459 209 2379
Fragments arranged by size:
2379 935 459 209 133
TatI w'GTAC_w
Cuts at: 272 323 3630 272
Size: 51 3307 757
Fragments arranged by size:
3307 757 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 366 1610 366
Size: 1244 2871
XmaI C'CCGG_G
Cuts at: 1135 1135
Size: 4115
XmnI GAAnn'nnTTC
Cuts at: 1084 1084
Size: 4115
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI