Restriction map for
pF1KB3870
(Circular) (Six-base) MAPSORT of: pF1KB3870.seq Check: 5102 from: 1 to: 4214
pF1KB3870 4214 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 374 1252 374
Size: 878 3336
Acc65I G'GTAC_C
Cuts at: 1230 1230
Size: 4214
AclI AA'CG_TT
Cuts at: 4054 4054
Size: 4214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 946 994 2039 2471 2824 3486 413
Size: 533 48 1045 432 353 662 1141
Fragments arranged by size:
1141 1045 662 533 432 353 48
AflIII A'CryG_T
Cuts at: 761 1566 3366 3647 761
Size: 805 1800 281 1328
Fragments arranged by size:
1800 1328 805 281
AlwNI CAG_nnn'CTG
Cuts at: 924 1519 2957 924
Size: 595 1438 2181
Fragments arranged by size:
2181 1438 595
ApaLI G'TGCA_C
Cuts at: 3052 3052
Size: 4214
ApoI r'AATT_y
Cuts at: 1218 3567 3823 1218
Size: 2349 256 1609
Fragments arranged by size:
2349 1609 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 169 1234 169
Size: 1065 3149
BamHI G'GATC_C
Cuts at: 1239 1239
Size: 4214
BanI G'GyrC_C
Cuts at: 823 956 1037 1230 1644 1892 1927 823
Size: 133 81 193 414 248 35 3110
Fragments arranged by size:
3110 414 248 193 133 81 35
BanII G_rGCy'C
Cuts at: 133 166 682 1228 2258 133
Size: 33 516 546 1030 2089
Fragments arranged by size:
2089 1030 546 516 33
BbeI G_GCGC'C
Cuts at: 827 960 1648 1896 827
Size: 133 688 248 3145
Fragments arranged by size:
3145 688 248 133
BbsI GAAGACnn'nnnn_
Cuts at: 1133 1133
Size: 4214
BciVI GTATCCnnnnn_n'
Cuts at: 122 2112 3168 4214 122
Size: 1990 1056 1046 122
Fragments arranged by size:
1990 1056 1046 122
BclI T'GATC_A
Cuts at: 1734 1734
Size: 4214
BglI GCCn_nnn'nGGC
Cuts at: 388 388
Size: 4214
BglII A'GATC_T
Cuts at: 461 1729 3766 461
Size: 1268 2037 909
Fragments arranged by size:
2037 1268 909
BlpI GC'TnA_GC
Cuts at: 1326 1326
Size: 4214
Bme1580I G_kGCm'C
Cuts at: 667 1839 1932 3056 667
Size: 1172 93 1124 1825
Fragments arranged by size:
1825 1172 1124 93
BmgBI CAC'GTC
Cuts at: 633 668 633
Size: 35 4179
BmrI ACTGGGnnnn_n'
Cuts at: 412 669 1426 1507 1842 3986 412
Size: 257 757 81 335 2144 640
Fragments arranged by size:
2144 757 640 335 257 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 720 2581 720
Size: 1861 2353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 559 574 696 199
Size: 360 15 122 3717
Fragments arranged by size:
3717 360 122 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 1390 2269 2983 3281 879
Size: 511 879 714 298 1812
Fragments arranged by size:
1812 879 714 511 298
BsaI GGTCTCn'nnnn_
Cuts at: 258 604 3918 258
Size: 346 3314 554
Fragments arranged by size:
3314 554 346
BsaAI yAC'GTr
Cuts at: 561 1567 561
Size: 1006 3208
BsaBI GATnn'nnATC
Cuts at: 6 1728 3765 6
Size: 1722 2037 455
Fragments arranged by size:
2037 1722 455
BsaHI Gr'CG_yC
Cuts at: 824 957 1645 1893 2595 4091 824
Size: 133 688 248 702 1496 947
Fragments arranged by size:
1496 947 702 688 248 133
BsaWI w'CCGG_w
Cuts at: 1403 1924 2602 3013 3160 3434 1403
Size: 521 678 411 147 274 2183
Fragments arranged by size:
2183 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 961 961
Size: 4214
BseYI C'CCAG_C
Cuts at: 1019 1639 2397 3062 3470 1019
Size: 620 758 665 408 1763
Fragments arranged by size:
1763 758 665 620 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 993 808
Size: 185 4029
BsiEI CG_ry'CG
Cuts at: 104 1756 1802 3032 104
Size: 1652 46 1230 1286
Fragments arranged by size:
1652 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 682 1070 1228 2006 2196 3056 682
Size: 388 158 778 190 860 1840
Fragments arranged by size:
1840 860 778 388 190 158
BsmBI CGTCTCn'nnnn_
Cuts at: 478 478
Size: 4214
Bsp1286I G_dGCh'C
Cuts at: 133 166 667 682 1070 1228 1839 1932 2006
Size: 33 501 15 388 158 611 93 74
Cuts at: 2006 2196 2258 3056 133
Size: 190 62 798 1291
Fragments arranged by size:
1291 798 611 501 388 190 158 93
74 62 33 15
BspHI T'CATG_A
Cuts at: 2646 2646
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1264 1478 1780 2161 1264
Size: 214 302 381 3317
Fragments arranged by size:
3317 381 302 214
BsrBI CCG'CTC
Cuts at: 50 492 2506 2560 4044 4209 50
Size: 442 2014 54 1484 165 55
Fragments arranged by size:
2014 1484 442 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2126 2126
Size: 4214
BsrFI r'CCGG_y
Cuts at: 382 953 2212 2393 382
Size: 571 1259 181 2203
Fragments arranged by size:
2203 1259 571 181
BssSI C'ACGA_G
Cuts at: 1153 2485 3193 1153
Size: 1332 708 2174
Fragments arranged by size:
2174 1332 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1591 1591
Size: 4214
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstEII G'GTnAC_C
Cuts at: 279 279
Size: 4214
BstYI r'GATC_y
Cuts at: 1 461 935 1204 1239 1706 1729 2064 2310
Size: 460 474 269 35 467 23 335 246
Cuts at: 2310 2616 2628 2714 3766 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 474 467 460 449 335 306 269
246 86 35 23 12
Bsu36I CC'TnA_GG
Cuts at: 1042 1042
Size: 4214
BtgI C'CryG_G
Cuts at: 683 707 683
Size: 24 4190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 975 2164 2345 2508 975
Size: 1189 181 163 2681
Fragments arranged by size:
2681 1189 181 163
BtsI GCAGTG_nn'
Cuts at: 1457 1457
Size: 4214
DraI TTT'AAA
Cuts at: 1213 1213
Size: 4214
DrdI GACnn_nn'nnGTC
Cuts at: 1920 3264 1920
Size: 1344 2870
EaeI y'GGCC_r
Cuts at: 650 1025 1799 1973 2364 2391 3736 650
Size: 375 774 174 391 27 1345 1128
Fragments arranged by size:
1345 1128 774 391 375 174 27
EagI C'GGCC_G
Cuts at: 1799 1799
Size: 4214
EarI CTCTTCn'nnn_
Cuts at: 361 602 2237 2447 361
Size: 241 1635 210 2128
Fragments arranged by size:
2128 1635 241 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 488 496 2422 3164 3310 4024 488
Size: 8 1926 742 146 714 678
Fragments arranged by size:
1926 742 714 678 146 8
EcoICRI GAG'CTC
Cuts at: 680 1226 680
Size: 546 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 413 559 574 696 946 994 2039 2471
Size: 214 146 15 122 250 48 1045 432
Cuts at: 2471 2824 3486 199
Size: 353 662 927
Fragments arranged by size:
1045 927 662 432 353 250 214 146
122 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 943 943
Size: 4214
EcoO109I rG'GnC_Cy
Cuts at: 495 1076 1122 1353 495
Size: 581 46 231 3356
Fragments arranged by size:
3356 581 231 46
EcoRI G'AATT_C
Cuts at: 1218 1218
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 1995 1995
Size: 4214
HaeII r_GCGC'y
Cuts at: 827 960 1002 1648 1896 3126 3897 827
Size: 133 42 646 248 1230 771 1144
Fragments arranged by size:
1230 1144 771 646 248 133 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 281 313 1189 1221 2056 2086 2088 2118 281
Size: 32 876 32 835 30 2 30 2377
Fragments arranged by size:
2377 876 835 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1253 1253
Size: 4214
HindIII A'AGCT_T
Cuts at: 530 530
Size: 4214
KasI G'GCGC_C
Cuts at: 823 956 1644 1892 823
Size: 133 688 248 3145
Fragments arranged by size:
3145 688 248 133
KpnI G_GTAC'C
Cuts at: 1234 1234
Size: 4214
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2973 3157 2973
Size: 184 4030
MscI TGG'CCA
Cuts at: 652 1027 1975 652
Size: 375 948 2891
Fragments arranged by size:
2891 948 375
MslI CAynn'nnrTG
Cuts at: 601 2203 2330 601
Size: 1602 127 2485
Fragments arranged by size:
2485 1602 127
MspA1I CmG'CkG
Cuts at: 552 792 1023 1325 1639 1999 2783 3028 552
Size: 240 231 302 314 360 784 245 1738
Fragments arranged by size:
1738 784 360 314 302 245 240 231
NaeI GCC'GGC
Cuts at: 384 955 2395 384
Size: 571 1440 2203
Fragments arranged by size:
2203 1440 571
NarI GG'CG_CC
Cuts at: 824 957 1645 1893 824
Size: 133 688 248 3145
Fragments arranged by size:
3145 688 248 133
NcoI C'CATG_G
Cuts at: 683 683
Size: 4214
NgoMIV G'CCGG_C
Cuts at: 382 953 2393 382
Size: 571 1440 2203
Fragments arranged by size:
2203 1440 571
NspI r_CATG'y
Cuts at: 765 1267 1767 3370 765
Size: 502 500 1603 1609
Fragments arranged by size:
1609 1603 502 500
PciI A'CATG_T
Cuts at: 761 3366 761
Size: 2605 1609
PflMI CCAn_nnn'nTGG
Cuts at: 648 659 833 1034 1098 648
Size: 11 174 201 64 3764
Fragments arranged by size:
3764 201 174 64 11
PfoI T'CCnGG_A
Cuts at: 502 502
Size: 4214
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 4182
PpuMI rG'GwC_Cy
Cuts at: 1122 1122
Size: 4214
PshAI GACnn'nnGTC
Cuts at: 631 631
Size: 4214
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 748 780 748
Size: 32 4182
PstI C_TGCA'G
Cuts at: 551 986 1261 1946 551
Size: 435 275 685 2819
Fragments arranged by size:
2819 685 435 275
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 552 792 1023 1639 1999 552
Size: 240 231 616 360 2767
Fragments arranged by size:
2767 616 360 240 231
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 682 1228 682
Size: 546 3668
SalI G'TCGA_C
Cuts at: 1251 1251
Size: 4214
SapI GCTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 186 3731 186
Size: 3545 669
SexAI A'CCwGG_T
Cuts at: 832 832
Size: 4214
SfcI C'TryA_G
Cuts at: 33 180 547 982 1185 1200 1257 1942 2910
Size: 147 367 435 203 15 57 685 968
Cuts at: 2910 3101 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 435 367 203 191 147
57 15
SfoI GGC'GCC
Cuts at: 825 958 1646 1894 825
Size: 133 688 248 3145
Fragments arranged by size:
3145 688 248 133
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 171 1236 171
Size: 1065 3149
SmlI C'TyrA_G
Cuts at: 894 1369 2284 2998 3260 894
Size: 475 915 714 262 1848
Fragments arranged by size:
1848 915 714 475 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4214
SphI G_CATG'C
Cuts at: 1267 1767 1267
Size: 500 3714
StuI AGG'CCT
Cuts at: 700 700
Size: 4214
StyI C'CwwG_G
Cuts at: 329 683 1348 1702 329
Size: 354 665 354 2841
Fragments arranged by size:
2841 665 354 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 267 1797 1930 2389 2598 267
Size: 1530 133 459 209 1883
Fragments arranged by size:
1883 1530 459 209 133
TatI w'GTAC_w
Cuts at: 184 656 3729 184
Size: 472 3073 669
Fragments arranged by size:
3073 669 472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 263 295 3731 3763 263
Size: 32 3436 32 714
Fragments arranged by size:
3436 714 32 32
Tth111I GACn'n_nGTC
Cuts at: 834 2011 834
Size: 1177 3037
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 690 1709 600
Size: 90 1019 3105
Fragments arranged by size:
3105 1019 90
XmaI C'CCGG_G
Cuts at: 169 1234 169
Size: 1065 3149
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BmtI Bpu10I BsaXI BsiWI BsmI BspEI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PsiI PspOMI PspXI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI