Restriction map for
pF1KB3889
(Circular) (Six-base) MAPSORT of: pF1KB3889.seq Check: 2495 from: 1 to: 5537
pF1KB3889 5537 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2046 2046
Size: 5537
AatII G_ACGT'C
Cuts at: 1207 1207
Size: 5537
AccI GT'mk_AC
Cuts at: 2575 2575
Size: 5537
Acc65I G'GTAC_C
Cuts at: 2102 2553 2102
Size: 451 5086
AclI AA'CG_TT
Cuts at: 5377 5377
Size: 5537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 699 763 1423 1749 1828 3362 3794 4147 4809
Size: 64 660 326 79 1534 432 353 662
Cuts at: 4809 699
Size: 1427
Fragments arranged by size:
1534 1427 662 660 432 353 326 79
64
AfeI AGC'GCT
Cuts at: 1534 2375 1534
Size: 841 4696
AflIII A'CryG_T
Cuts at: 2889 4689 4970 2889
Size: 1800 281 3456
Fragments arranged by size:
3456 1800 281
AgeI A'CCGG_T
Cuts at: 659 659
Size: 5537
AhdI GACnn_n'nnGTC
Cuts at: 359 359
Size: 5537
AleI CACnn'nnGTG
Cuts at: 1670 1670
Size: 5537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1855 1855
Size: 5537
AlwNI CAG_nnn'CTG
Cuts at: 940 1647 1713 1842 2842 4280 940
Size: 707 66 129 1000 1438 2197
Fragments arranged by size:
2197 1438 1000 707 129 66
ApaI G_GGCC'C
Cuts at: 979 1142 1629 979
Size: 163 487 4887
Fragments arranged by size:
4887 487 163
ApaLI G'TGCA_C
Cuts at: 568 4375 568
Size: 3807 1730
ApoI r'AATT_y
Cuts at: 2541 4890 5146 2541
Size: 2349 256 2932
Fragments arranged by size:
2932 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5537
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
AvaI C'yCGr_G
Cuts at: 267 1141 1988 2338 2557 267
Size: 874 847 350 219 3247
Fragments arranged by size:
3247 874 847 350 219
BamHI G'GATC_C
Cuts at: 2562 2562
Size: 5537
BanI G'GyrC_C
Cuts at: 238 1381 1735 2102 2553 2967 3215 3250 238
Size: 1143 354 367 451 414 248 35 2525
Fragments arranged by size:
2525 1143 451 414 367 354 248 35
BanII G_rGCy'C
Cuts at: 979 1142 1629 2551 3581 979
Size: 163 487 922 1030 2935
Fragments arranged by size:
2935 1030 922 487 163
BbeI G_GCGC'C
Cuts at: 2971 3219 2971
Size: 248 5289
BbsI GAAGACnn'nnnn_
Cuts at: 1647 1647
Size: 5537
BbvCI CC'TCA_GC
Cuts at: 328 1725 328
Size: 1397 4140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 786 820 786
Size: 34 5503
BciVI GTATCCnnnnn_n'
Cuts at: 3435 4491 5537 3435
Size: 1056 1046 3435
Fragments arranged by size:
3435 1056 1046
BclI T'GATC_A
Cuts at: 3057 3057
Size: 5537
BglI GCCn_nnn'nGGC
Cuts at: 271 364 442 451 271
Size: 93 78 9 5357
Fragments arranged by size:
5357 93 78 9
BglII A'GATC_T
Cuts at: 758 3052 5089 758
Size: 2294 2037 1206
Fragments arranged by size:
2294 2037 1206
BlpI GC'TnA_GC
Cuts at: 739 2419 2649 739
Size: 1680 230 3627
Fragments arranged by size:
3627 1680 230
Bme1580I G_kGCm'C
Cuts at: 572 979 1142 1384 1629 1755 1793 3162 3255
Size: 407 163 242 245 126 38 1369 93
Cuts at: 3255 4379 572
Size: 1124 1730
Fragments arranged by size:
1730 1369 1124 407 245 242 163 126
93 38
BmrI ACTGGGnnnn_n'
Cuts at: 1982 2749 2830 3165 5309 1982
Size: 767 81 335 2144 2210
Fragments arranged by size:
2210 2144 767 335 81
BmtI G_CTAG'C
Cuts at: 481 481
Size: 5537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1089 2014 3904 1089
Size: 925 1890 2722
Fragments arranged by size:
2722 1890 925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1335 1741 2416 310
Size: 1025 406 675 3431
Fragments arranged by size:
3431 1025 675 406
Bpu10I CC'TnA_GC
Cuts at: 328 1352 1725 328
Size: 1024 373 4140
Fragments arranged by size:
4140 1024 373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2343 2398 2713 3592 4306 4604 1759
Size: 584 55 315 879 714 298 2692
Fragments arranged by size:
2692 879 714 584 315 298 55
BsaI GGTCTCn'nnnn_
Cuts at: 109 5241 109
Size: 5132 405
BsaAI yAC'GTr
Cuts at: 2267 2890 2267
Size: 623 4914
BsaBI GATnn'nnATC
Cuts at: 6 763 3051 5088 6
Size: 757 2288 2037 455
Fragments arranged by size:
2288 2037 757 455
BsaHI Gr'CG_yC
Cuts at: 1204 2968 3216 3918 5414 1204
Size: 1764 248 702 1496 1327
Fragments arranged by size:
1764 1496 1327 702 248
BsaWI w'CCGG_w
Cuts at: 659 1127 1262 1360 2174 2726 3247 3925 4336
Size: 468 135 98 814 552 521 678 411
Cuts at: 4336 4483 4757 659
Size: 147 274 1439
Fragments arranged by size:
1439 814 678 552 521 468 411 274
147 135 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 451 481 841 871 451
Size: 30 360 30 5117
Fragments arranged by size:
5117 360 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 606 862 1126 2047 2065 2356 606
Size: 256 264 921 18 291 3787
Fragments arranged by size:
3787 921 291 264 256 18
BseYI C'CCAG_C
Cuts at: 860 864 965 1647 2115 2310 2962 3720 4385
Size: 4 101 682 468 195 652 758 665
Cuts at: 4385 4793 860
Size: 408 1604
Fragments arranged by size:
1604 758 682 665 652 468 408 195
101 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 984 1422 1924 984
Size: 438 502 4597
Fragments arranged by size:
4597 502 438
BsiEI CG_ry'CG
Cuts at: 104 125 750 2489 3079 3125 4355 104
Size: 21 625 1739 590 46 1230 1286
Fragments arranged by size:
1739 1286 1230 625 590 46 21
BsiHKAI G_wGCw'C
Cuts at: 572 2321 2551 3329 3519 4379 572
Size: 1749 230 778 190 860 1730
Fragments arranged by size:
1749 1730 860 778 230 190
Bsp1286I G_dGCh'C
Cuts at: 572 979 1142 1384 1629 1755 1793 2321 2551
Size: 407 163 242 245 126 38 528 230
Cuts at: 2551 3162 3255 3329 3519 3581 4379 572
Size: 611 93 74 190 62 798 1730
Fragments arranged by size:
1730 798 611 528 407 245 242 230
190 163 126 93 74 62 38
BspEI T'CCGG_A
Cuts at: 1262 2174 1262
Size: 912 4625
BspHI T'CATG_A
Cuts at: 3969 3969
Size: 5537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2046 2093 2114 2587 2801 3103 3484 2046
Size: 47 21 473 214 302 381 4099
Fragments arranged by size:
4099 473 381 302 214 47 21
BsrBI CCG'CTC
Cuts at: 50 2403 3829 3883 5367 5532 50
Size: 2353 1426 54 1484 165 55
Fragments arranged by size:
2353 1484 1426 165 55 54
BsrDI GCAATG_nn'
Cuts at: 367 3449 367
Size: 3082 2455
BsrFI r'CCGG_y
Cuts at: 659 1018 1667 1949 2484 3535 3716 659
Size: 359 649 282 535 1051 181 2480
Fragments arranged by size:
2480 1051 649 535 359 282 181
BsrGI T'GTAC_A
Cuts at: 953 953
Size: 5537
BssHII G'CGCG_C
Cuts at: 2522 2522
Size: 5537
BssSI C'ACGA_G
Cuts at: 795 1864 1918 2293 3808 4516 795
Size: 1069 54 375 1515 708 1816
Fragments arranged by size:
1816 1515 1069 708 375 54
BstAPI GCAn_nnn'nTGC
Cuts at: 1388 2914 1388
Size: 1526 4011
BstBI TT'CG_AA
Cuts at: 3898 3898
Size: 5537
BstXI CCAn_nnnn'nTGG
Cuts at: 1672 2133 1672
Size: 461 5076
BstYI r'GATC_y
Cuts at: 1 758 1217 2562 3029 3052 3387 3633 3939
Size: 757 459 1345 467 23 335 246 306
Cuts at: 3939 3951 4037 5089 1
Size: 12 86 1052 449
Fragments arranged by size:
1345 1052 757 467 459 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2186 2186
Size: 5537
BtgI C'CryG_G
Cuts at: 107 119 440 449 623 1224 1700 1718 1827
Size: 12 321 9 174 601 476 18 109
Cuts at: 1827 107
Size: 3817
Fragments arranged by size:
3817 601 476 321 174 109 18 12
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3487 3668 3831 3487
Size: 181 163 5193
Fragments arranged by size:
5193 181 163
BtsI GCAGTG_nn'
Cuts at: 314 649 952 996 1941 2286 2780 314
Size: 335 303 44 945 345 494 3071
Fragments arranged by size:
3071 945 494 345 335 303 44
DraI TTT'AAA
Cuts at: 2536 2536
Size: 5537
DraIII CAC_nnn'GTG
Cuts at: 576 1377 2151 576
Size: 801 774 3962
Fragments arranged by size:
3962 801 774
DrdI GACnn_nn'nnGTC
Cuts at: 1990 3243 4587 1990
Size: 1253 1344 2940
Fragments arranged by size:
2940 1344 1253
EaeI y'GGCC_r
Cuts at: 122 213 443 620 726 747 1227 1332 1589
Size: 91 230 177 106 21 480 105 257
Cuts at: 1589 1613 1715 2123 2486 3122 3296 3687 3714
Size: 24 102 408 363 636 174 391 27
Cuts at: 3714 5059 122
Size: 1345 600
Fragments arranged by size:
1345 636 600 480 408 391 363 257
230 177 174 106 105 102 91 27
24 21
EagI C'GGCC_G
Cuts at: 122 747 2486 3122 122
Size: 625 1739 636 2537
Fragments arranged by size:
2537 1739 636 625
EarI CTCTTCn'nnn_
Cuts at: 229 616 1463 3560 3770 229
Size: 387 847 2097 210 1996
Fragments arranged by size:
2097 1996 847 387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 203 2035 3745 4487 4633 5347 203
Size: 1832 1710 742 146 714 393
Fragments arranged by size:
1832 1710 742 714 393 146
EcoICRI GAG'CTC
Cuts at: 2549 2549
Size: 5537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 699 763 1335 1423 1741 1749 1828 2416
Size: 389 64 572 88 318 8 79 588
Cuts at: 2416 3362 3794 4147 4809 310
Size: 946 432 353 662 1038
Fragments arranged by size:
1038 946 662 588 572 432 389 353
318 88 79 64 8
EcoNI CCTnn'n_nnAGG
Cuts at: 418 1513 2002 418
Size: 1095 489 3953
Fragments arranged by size:
3953 1095 489
EcoO109I rG'GnC_Cy
Cuts at: 774 976 1626 2181 2244 2334 2382 2426 2676
Size: 202 650 555 63 90 48 44 250
Cuts at: 2676 774
Size: 3635
Fragments arranged by size:
3635 650 555 250 202 90 63 48
44
EcoRI G'AATT_C
Cuts at: 2541 2541
Size: 5537
EcoRV GAT'ATC
Cuts at: 1239 1239
Size: 5537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1428 1833 3556 1428
Size: 405 1723 3409
Fragments arranged by size:
3409 1723 405
FspI TGC'GCA
Cuts at: 3318 3318
Size: 5537
HaeII r_GCGC'y
Cuts at: 698 722 1536 2116 2377 2971 3219 4449 5220
Size: 24 814 580 261 594 248 1230 771
Cuts at: 5220 698
Size: 1015
Fragments arranged by size:
1230 1015 814 771 594 580 261 248
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 345 377 451 483 841 873 3379 3409 3411
Size: 32 74 32 358 32 2506 30 2
Cuts at: 3411 3441 345
Size: 30 2441
Fragments arranged by size:
2506 2441 358 74 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 249 2576 249
Size: 2327 3210
KasI G'GCGC_C
Cuts at: 2967 3215 2967
Size: 248 5289
KpnI G_GTAC'C
Cuts at: 2106 2557 2106
Size: 451 5086
MluI A'CGCG_T
Cuts at: 4970 4970
Size: 5537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2244 4296 4480 2244
Size: 2052 184 3301
Fragments arranged by size:
3301 2052 184
MscI TGG'CCA
Cuts at: 215 445 1334 1591 1717 2125 3298 215
Size: 230 889 257 126 408 1173 2454
Fragments arranged by size:
2454 1173 889 408 257 230 126
MslI CAynn'nnrTG
Cuts at: 640 1670 3526 3653 640
Size: 1030 1856 127 2524
Fragments arranged by size:
2524 1856 1030 127
MspA1I CmG'CkG
Cuts at: 121 201 864 901 1286 1644 1729 1769 1839
Size: 80 663 37 385 358 85 40 70
Cuts at: 1839 1850 2415 2648 2962 3322 4106 4351 121
Size: 11 565 233 314 360 784 245 1307
Fragments arranged by size:
1307 784 663 565 385 360 358 314
245 233 85 80 70 40 37 11
NaeI GCC'GGC
Cuts at: 1020 1951 2486 3718 1020
Size: 931 535 1232 2839
Fragments arranged by size:
2839 1232 931 535
NarI GG'CG_CC
Cuts at: 2968 3216 2968
Size: 248 5289
NcoI C'CATG_G
Cuts at: 107 449 1700 107
Size: 342 1251 3944
Fragments arranged by size:
3944 1251 342
NgoMIV G'CCGG_C
Cuts at: 1018 1949 2484 3716 1018
Size: 931 535 1232 2839
Fragments arranged by size:
2839 1232 931 535
NheI G'CTAG_C
Cuts at: 477 477
Size: 5537
NspI r_CATG'y
Cuts at: 2590 3090 4693 2590
Size: 500 1603 3434
Fragments arranged by size:
3434 1603 500
PasI CC'CwG_GG
Cuts at: 979 2504 979
Size: 1525 4012
PciI A'CATG_T
Cuts at: 4689 4689
Size: 5537
PflMI CCAn_nnn'nTGG
Cuts at: 1622 1647 1713 2504 1622
Size: 25 66 791 4655
Fragments arranged by size:
4655 791 66 25
PfoI T'CCnGG_A
Cuts at: 1757 1757
Size: 5537
PmeI GTTT'AAAC
Cuts at: 2536 2536
Size: 5537
PmlI CAC'GTG
Cuts at: 2267 2267
Size: 5537
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2295 3983 4015 2263
Size: 32 1688 32 3785
Fragments arranged by size:
3785 1688 32 32
PpuMI rG'GwC_Cy
Cuts at: 774 2181 2426 774
Size: 1407 245 3885
Fragments arranged by size:
3885 1407 245
PspOMI G'GGCC_C
Cuts at: 975 1138 1625 975
Size: 163 487 4887
Fragments arranged by size:
4887 487 163
PstI C_TGCA'G
Cuts at: 656 1088 1202 1517 1948 2584 3269 656
Size: 432 114 315 431 636 685 2924
Fragments arranged by size:
2924 685 636 432 431 315 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5537
PvuII CAG'CTG
Cuts at: 201 864 901 1286 1644 1729 1769 1839 2962
Size: 663 37 385 358 85 40 70 1123
Cuts at: 2962 3322 201
Size: 360 2416
Fragments arranged by size:
2416 1123 663 385 360 358 85 70
40 37
RsrII CG'GwC_CG
Cuts at: 3732 3732
Size: 5537
SacI G_AGCT'C
Cuts at: 2551 2551
Size: 5537
SacII CC_GC'GG
Cuts at: 122 122
Size: 5537
SalI G'TCGA_C
Cuts at: 2574 2574
Size: 5537
SanDI GG'GwC_CC
Cuts at: 2181 2181
Size: 5537
SapI GCTCTTCn'nnn_
Cuts at: 616 3560 3770 616
Size: 2944 210 2383
Fragments arranged by size:
2944 2383 210
SbfI CC_TGCA'GG
Cuts at: 1517 2584 1517
Size: 1067 4470
ScaI AGT'ACT
Cuts at: 5054 5054
Size: 5537
SexAI A'CCwGG_T
Cuts at: 1706 1706
Size: 5537
SfcI C'TryA_G
Cuts at: 33 652 690 1084 1104 1198 1513 1944 2580
Size: 619 38 394 20 94 315 431 636
Cuts at: 2580 3265 4233 4424 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 636 619 431 394 315
191 94 38 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 271 442 451 271
Size: 171 9 5357
Fragments arranged by size:
5357 171 9
SfoI GGC'GCC
Cuts at: 2969 3217 2969
Size: 248 5289
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
SgrAI Cr'CCGG_yG
Cuts at: 1018 1667 1018
Size: 649 4888
SmaI CCC'GGG
Cuts at: 2559 2559
Size: 5537
SmlI C'TyrA_G
Cuts at: 1774 2322 2377 2692 3607 4321 4583 1774
Size: 548 55 315 915 714 262 2728
Fragments arranged by size:
2728 915 714 548 315 262 55
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5537
SphI G_CATG'C
Cuts at: 2590 3090 2590
Size: 500 5037
StuI AGG'CCT
Cuts at: 884 2191 884
Size: 1307 4230
StyI C'CwwG_G
Cuts at: 107 449 909 1292 1700 2671 3025 107
Size: 342 460 383 408 971 354 2619
Fragments arranged by size:
2619 971 460 408 383 354 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 856 1639 2029 2302 3120 3253 3712 3921 856
Size: 783 390 273 818 133 459 209 2472
Fragments arranged by size:
2472 818 783 459 390 273 209 133
TatI w'GTAC_w
Cuts at: 887 953 5052 887
Size: 66 4099 1372
Fragments arranged by size:
4099 1372 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 530 562 2443 2475 5054 5086 530
Size: 32 1881 32 2579 32 981
Fragments arranged by size:
2579 1881 981 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1927 3334 1927
Size: 1407 4130
XbaI T'CTAG_A
Cuts at: 2568 2568
Size: 5537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 153 1969 3032 153
Size: 1816 1063 2658
Fragments arranged by size:
2658 1816 1063
XmaI C'CCGG_G
Cuts at: 2557 2557
Size: 5537
XmnI GAAnn'nnTTC
Cuts at: 1093 1093
Size: 5537
ZraI GAC'GTC
Cuts at: 1205 1205
Size: 5537
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BfrBI BmgBI BsiWI
BsmI BsmBI BstEII BstZ17I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PshAI PsiI PspXI PsrI SnaBI SrfI SspI SwaI
XhoI