Restriction map for
pF1KB3912
(Circular) (Six-base) MAPSORT of: pF1KB3912.seq Check: 2856 from: 1 to: 3572
pF1KB3912 3572 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 610 610
Size: 3572
Acc65I G'GTAC_C
Cuts at: 588 588
Size: 3572
AclI AA'CG_TT
Cuts at: 3412 3412
Size: 3572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1397 1829 2182 2844 277
Size: 1120 432 353 662 1005
Fragments arranged by size:
1120 1005 662 432 353
AflIII A'CryG_T
Cuts at: 924 2724 3005 924
Size: 1800 281 1491
Fragments arranged by size:
1800 1491 281
AlwNI CAG_nnn'CTG
Cuts at: 877 2315 877
Size: 1438 2134
ApaLI G'TGCA_C
Cuts at: 2410 2410
Size: 3572
ApoI r'AATT_y
Cuts at: 576 2925 3181 576
Size: 2349 256 967
Fragments arranged by size:
2349 967 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
AvaI C'yCGr_G
Cuts at: 592 592
Size: 3572
BamHI G'GATC_C
Cuts at: 597 597
Size: 3572
BanI G'GyrC_C
Cuts at: 363 588 1002 1250 1285 363
Size: 225 414 248 35 2650
Fragments arranged by size:
2650 414 248 225 35
BanII G_rGCy'C
Cuts at: 586 1616 586
Size: 1030 2542
BbeI G_GCGC'C
Cuts at: 367 1006 1254 367
Size: 639 248 2685
Fragments arranged by size:
2685 639 248
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 3572
BciVI GTATCCnnnnn_n'
Cuts at: 1470 2526 3572 1470
Size: 1056 1046 1470
Fragments arranged by size:
1470 1056 1046
BclI T'GATC_A
Cuts at: 1092 1092
Size: 3572
BglII A'GATC_T
Cuts at: 1087 3124 1087
Size: 2037 1535
BlpI GC'TnA_GC
Cuts at: 275 684 275
Size: 409 3163
Bme1580I G_kGCm'C
Cuts at: 477 1197 1290 2414 477
Size: 720 93 1124 1635
Fragments arranged by size:
1635 1124 720 93
BmrI ACTGGGnnnn_n'
Cuts at: 784 865 1200 3344 784
Size: 81 335 2144 1012
Fragments arranged by size:
2144 1012 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 383 1939 383
Size: 1556 2016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 442 333
Size: 109 3463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 748 1627 2341 2639 427
Size: 321 879 714 298 1360
Fragments arranged by size:
1360 879 714 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 3276 3276
Size: 3572
BsaAI yAC'GTr
Cuts at: 925 925
Size: 3572
BsaBI GATnn'nnATC
Cuts at: 6 1086 3123 6
Size: 1080 2037 455
Fragments arranged by size:
2037 1080 455
BsaHI Gr'CG_yC
Cuts at: 364 1003 1251 1953 3449 364
Size: 639 248 702 1496 487
Fragments arranged by size:
1496 702 639 487 248
BsaWI w'CCGG_w
Cuts at: 255 398 761 1282 1960 2371 2518 2792 255
Size: 143 363 521 678 411 147 274 1035
Fragments arranged by size:
1035 678 521 411 363 274 147 143
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 183
Size: 3572
BseYI C'CCAG_C
Cuts at: 476 997 1755 2420 2828 476
Size: 521 758 665 408 1220
Fragments arranged by size:
1220 758 665 521 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 563
Size: 3572
BsiEI CG_ry'CG
Cuts at: 104 1114 1160 2390 104
Size: 1010 46 1230 1286
Fragments arranged by size:
1286 1230 1010 46
BsiHKAI G_wGCw'C
Cuts at: 586 1364 1554 2414 586
Size: 778 190 860 1744
Fragments arranged by size:
1744 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 253 253
Size: 3572
Bsp1286I G_dGCh'C
Cuts at: 477 586 1197 1290 1364 1554 1616 2414 477
Size: 109 611 93 74 190 62 798 1635
Fragments arranged by size:
1635 798 611 190 109 93 74 62
BspEI T'CCGG_A
Cuts at: 398 398
Size: 3572
BspHI T'CATG_A
Cuts at: 2004 2004
Size: 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 836 1138 1519 622
Size: 214 302 381 2675
Fragments arranged by size:
2675 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1864 1918 3402 3567 50
Size: 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1484 1484
Size: 3572
BsrFI r'CCGG_y
Cuts at: 1570 1751 1570
Size: 181 3391
BssSI C'ACGA_G
Cuts at: 1843 2551 1843
Size: 708 2864
BstAPI GCAn_nnn'nTGC
Cuts at: 949 949
Size: 3572
BstBI TT'CG_AA
Cuts at: 1933 1933
Size: 3572
BstYI r'GATC_y
Cuts at: 1 597 1064 1087 1422 1668 1974 1986 2072
Size: 596 467 23 335 246 306 12 86
Cuts at: 2072 3124 1
Size: 1052 449
Fragments arranged by size:
1052 596 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 245 107
Size: 138 3434
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1522 1703 1866 1522
Size: 181 163 3228
Fragments arranged by size:
3228 181 163
BtsI GCAGTG_nn'
Cuts at: 815 815
Size: 3572
DraI TTT'AAA
Cuts at: 571 571
Size: 3572
DrdI GACnn_nn'nnGTC
Cuts at: 1278 2622 1278
Size: 1344 2228
EaeI y'GGCC_r
Cuts at: 1157 1331 1722 1749 3094 1157
Size: 174 391 27 1345 1635
Fragments arranged by size:
1635 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1157 1157
Size: 3572
EarI CTCTTCn'nnn_
Cuts at: 208 296 1595 1805 208
Size: 88 1299 210 1975
Fragments arranged by size:
1975 1299 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1780 2522 2668 3382 1780
Size: 742 146 714 1970
Fragments arranged by size:
1970 742 714 146
EcoICRI GAG'CTC
Cuts at: 584 584
Size: 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 333 442 1397 1829 2182 2844 277
Size: 56 109 955 432 353 662 1005
Fragments arranged by size:
1005 955 662 432 353 109 56
EcoNI CCTnn'n_nnAGG
Cuts at: 371 371
Size: 3572
EcoO109I rG'GnC_Cy
Cuts at: 711 711
Size: 3572
EcoRI G'AATT_C
Cuts at: 576 576
Size: 3572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 168 1591 168
Size: 1423 2149
FspI TGC'GCA
Cuts at: 435 1353 435
Size: 918 2654
HaeII r_GCGC'y
Cuts at: 217 367 483 1006 1254 2484 3255 217
Size: 150 116 523 248 1230 771 534
Fragments arranged by size:
1230 771 534 523 248 150 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 393 425 1414 1444 1446 1476 393
Size: 32 989 30 2 30 2489
Fragments arranged by size:
2489 989 32 30 30 2
HincII GTy'rAC
Cuts at: 611 611
Size: 3572
KasI G'GCGC_C
Cuts at: 363 1002 1250 363
Size: 639 248 2685
Fragments arranged by size:
2685 639 248
KpnI G_GTAC'C
Cuts at: 592 592
Size: 3572
MluI A'CGCG_T
Cuts at: 3005 3005
Size: 3572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 154 337 2331 2515 154
Size: 183 1994 184 1211
Fragments arranged by size:
1994 1211 184 183
MscI TGG'CCA
Cuts at: 1333 1333
Size: 3572
MslI CAynn'nnrTG
Cuts at: 490 1561 1688 490
Size: 1071 127 2374
Fragments arranged by size:
2374 1071 127
MspA1I CmG'CkG
Cuts at: 683 997 1357 2141 2386 683
Size: 314 360 784 245 1869
Fragments arranged by size:
1869 784 360 314 245
NaeI GCC'GGC
Cuts at: 1753 1753
Size: 3572
NarI GG'CG_CC
Cuts at: 364 1003 1251 364
Size: 639 248 2685
Fragments arranged by size:
2685 639 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3572
NgoMIV G'CCGG_C
Cuts at: 1751 1751
Size: 3572
NspI r_CATG'y
Cuts at: 495 625 1125 2728 495
Size: 130 500 1603 1339
Fragments arranged by size:
1603 1339 500 130
PciI A'CATG_T
Cuts at: 2724 2724
Size: 3572
PmeI GTTT'AAAC
Cuts at: 571 571
Size: 3572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2050 2018
Size: 32 3540
PstI C_TGCA'G
Cuts at: 619 1304 619
Size: 685 2887
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3572
PvuII CAG'CTG
Cuts at: 997 1357 997
Size: 360 3212
RsrII CG'GwC_CG
Cuts at: 1767 1767
Size: 3572
SacI G_AGCT'C
Cuts at: 586 586
Size: 3572
SalI G'TCGA_C
Cuts at: 609 609
Size: 3572
SapI GCTCTTCn'nnn_
Cuts at: 296 1595 1805 296
Size: 1299 210 2063
Fragments arranged by size:
2063 1299 210
SbfI CC_TGCA'GG
Cuts at: 619 619
Size: 3572
ScaI AGT'ACT
Cuts at: 3089 3089
Size: 3572
SfcI C'TryA_G
Cuts at: 33 558 615 1300 2268 2459 33
Size: 525 57 685 968 191 1146
Fragments arranged by size:
1146 968 685 525 191 57
SfoI GGC'GCC
Cuts at: 365 1004 1252 365
Size: 639 248 2685
Fragments arranged by size:
2685 639 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
SmaI CCC'GGG
Cuts at: 594 594
Size: 3572
SmlI C'TyrA_G
Cuts at: 442 727 1642 2356 2618 442
Size: 285 915 714 262 1396
Fragments arranged by size:
1396 915 714 285 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3572
SphI G_CATG'C
Cuts at: 625 1125 625
Size: 500 3072
StyI C'CwwG_G
Cuts at: 107 706 1060 107
Size: 599 354 2619
Fragments arranged by size:
2619 599 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1155 1288 1747 1956 1155
Size: 133 459 209 2771
Fragments arranged by size:
2771 459 209 133
TatI w'GTAC_w
Cuts at: 513 3087 513
Size: 2574 998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 3089 3121 172
Size: 32 2885 32 623
Fragments arranged by size:
2885 623 32 32
Tth111I GACn'n_nGTC
Cuts at: 1369 1369
Size: 3572
XbaI T'CTAG_A
Cuts at: 603 603
Size: 3572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1067 1067
Size: 3572
XmaI C'CCGG_G
Cuts at: 592 592
Size: 3572
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI