Restriction map for
pF1KB3935
(Circular) (Six-base) MAPSORT of: pF1KB3935.seq Check: 7186 from: 1 to: 4379
pF1KB3935 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1167 1167
Size: 4379
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 750 1395 750
Size: 645 3734
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 529 1027 1099 2204 2636 2989 3651 256
Size: 273 498 72 1105 432 353 662 984
Fragments arranged by size:
1105 984 662 498 432 353 273 72
AflIII A'CryG_T
Cuts at: 1731 3531 3812 1731
Size: 1800 281 2298
Fragments arranged by size:
2298 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 585 1684 3122 585
Size: 1099 1438 1842
Fragments arranged by size:
1842 1438 1099
ApaLI G'TGCA_C
Cuts at: 3217 3217
Size: 4379
ApoI r'AATT_y
Cuts at: 266 685 835 1383 3732 3988 266
Size: 419 150 548 2349 256 657
Fragments arranged by size:
2349 657 548 419 256 150
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 1399 1399
Size: 4379
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 750 1395 1809 2057 2092 750
Size: 645 414 248 35 3037
Fragments arranged by size:
3037 645 414 248 35
BanII G_rGCy'C
Cuts at: 1393 2423 1393
Size: 1030 3349
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 448 717 448
Size: 269 4110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 153 187 153
Size: 34 4345
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3333 4379 2277
Size: 1056 1046 2277
Fragments arranged by size:
2277 1056 1046
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BfrBI ATG'CAT
Cuts at: 223 223
Size: 4379
BglI GCCn_nnn'nGGC
Cuts at: 1360 1360
Size: 4379
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 637 2004 2097 3221 637
Size: 1367 93 1124 1795
Fragments arranged by size:
1795 1367 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 544 1311 1591 1672 2007 4151 544
Size: 767 280 81 335 2144 772
Fragments arranged by size:
2144 772 767 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1012 224
Size: 788 3591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1555 2434 3148 3446 448
Size: 1107 879 714 298 1381
Fragments arranged by size:
1381 1107 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4083 4083
Size: 4379
BsaAI yAC'GTr
Cuts at: 651 957 1732 651
Size: 306 775 3298
Fragments arranged by size:
3298 775 306
BsaBI GATnn'nnATC
Cuts at: 6 803 1893 3930 6
Size: 797 1090 2037 455
Fragments arranged by size:
2037 1090 797 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2760 4256 1810
Size: 248 702 1496 1933
Fragments arranged by size:
1933 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2767 3178 3325 3599 1568
Size: 521 678 411 147 274 2348
Fragments arranged by size:
2348 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 1354 541
Size: 813 3566
BseYI C'CCAG_C
Cuts at: 1804 2562 3227 3635 1804
Size: 758 665 408 2548
Fragments arranged by size:
2548 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1195
Size: 4379
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3197 104
Size: 1817 46 1230 1286
Fragments arranged by size:
1817 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1393 2171 2361 3221 1393
Size: 778 190 860 2551
Fragments arranged by size:
2551 860 778 190
BsmI GAATG_Cn'
Cuts at: 411 411
Size: 4379
Bsp1286I G_dGCh'C
Cuts at: 637 1393 2004 2097 2171 2361 2423 3221 637
Size: 756 611 93 74 190 62 798 1795
Fragments arranged by size:
1795 798 756 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2811 2811
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 902 1167 1323 1429 1643 1945 2326 337
Size: 565 265 156 106 214 302 381 2390
Fragments arranged by size:
2390 565 381 302 265 214 156 106
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 271 2291 186
Size: 85 2020 2274
Fragments arranged by size:
2274 2020 85
BsrFI r'CCGG_y
Cuts at: 1258 2377 2558 1258
Size: 1119 181 3079
Fragments arranged by size:
3079 1119 181
BssSI C'ACGA_G
Cuts at: 2650 3358 2650
Size: 708 3671
BstAPI GCAn_nnn'nTGC
Cuts at: 1329 1756 1329
Size: 427 3952
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstYI r'GATC_y
Cuts at: 1 1404 1871 1894 2229 2475 2781 2793 2879
Size: 1403 467 23 335 246 306 12 86
Cuts at: 2879 3931 1
Size: 1052 449
Fragments arranged by size:
1403 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 280 107
Size: 173 4206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4035
Fragments arranged by size:
4035 181 163
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
DraI TTT'AAA
Cuts at: 840 1378 840
Size: 538 3841
DrdI GACnn_nn'nnGTC
Cuts at: 2085 3429 2085
Size: 1344 3035
EaeI y'GGCC_r
Cuts at: 867 1292 1361 1964 2138 2529 2556 3901 867
Size: 425 69 603 174 391 27 1345 1345
Fragments arranged by size:
1345 1345 603 425 391 174 69 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 518 908 2402 2612 518
Size: 390 1494 210 2285
Fragments arranged by size:
2285 1494 390 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 3329 3475 4189 2587
Size: 742 146 714 2777
Fragments arranged by size:
2777 742 714 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 256 529 1012 1027 1099 2204 2636 2989
Size: 32 273 483 15 72 1105 432 353
Cuts at: 2989 3651 224
Size: 662 952
Fragments arranged by size:
1105 952 662 483 432 353 273 72
32 15
EcoNI CCTnn'n_nnAGG
Cuts at: 206 206
Size: 4379
EcoO109I rG'GnC_Cy
Cuts at: 132 459 1518 132
Size: 327 1059 2993
Fragments arranged by size:
2993 1059 327
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
EcoRV GAT'ATC
Cuts at: 936 936
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 1813 2061 3291 4062 1813
Size: 248 1230 771 2130
Fragments arranged by size:
2130 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 438 470 796 828 2221 2251 2253
Size: 32 301 32 326 32 1393 30 2
Cuts at: 2253 2283 105
Size: 30 2201
Fragments arranged by size:
2201 1393 326 301 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4379
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 754 1399 754
Size: 645 3734
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3138 3322 3138
Size: 184 4195
MscI TGG'CCA
Cuts at: 869 1294 2140 869
Size: 425 846 3108
Fragments arranged by size:
3108 846 425
MslI CAynn'nnrTG
Cuts at: 415 2368 2495 415
Size: 1953 127 2299
Fragments arranged by size:
2299 1953 127
MspA1I CmG'CkG
Cuts at: 582 1490 1804 2164 2948 3193 582
Size: 908 314 360 784 245 1768
Fragments arranged by size:
1768 908 784 360 314 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 107 107
Size: 4379
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NruI TCG'CGA
Cuts at: 163 163
Size: 4379
NsiI A_TGCA'T
Cuts at: 225 225
Size: 4379
NspI r_CATG'y
Cuts at: 1290 1432 1932 3535 1290
Size: 142 500 1603 2134
Fragments arranged by size:
2134 1603 500 142
PciI A'CATG_T
Cuts at: 3531 3531
Size: 4379
PflMI CCAn_nnn'nTGG
Cuts at: 585 585
Size: 4379
PfoI T'CCnGG_A
Cuts at: 201 201
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 4347
PshAI GACnn'nnGTC
Cuts at: 697 982 1278 697
Size: 285 296 3798
Fragments arranged by size:
3798 296 285
PstI C_TGCA'G
Cuts at: 1334 1426 2111 1334
Size: 92 685 3602
Fragments arranged by size:
3602 685 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 582 1804 2164 582
Size: 1222 360 2797
Fragments arranged by size:
2797 1222 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 908 2402 2612 908
Size: 1494 210 2675
Fragments arranged by size:
2675 1494 210
SbfI CC_TGCA'GG
Cuts at: 1334 1426 1334
Size: 92 4287
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SfcI C'TryA_G
Cuts at: 33 1330 1422 2107 3075 3266 33
Size: 1297 92 685 968 191 1146
Fragments arranged by size:
1297 1146 968 685 191 92
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SgrAI Cr'CCGG_yG
Cuts at: 1258 1258
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 463 1534 2449 3163 3425 463
Size: 1071 915 714 262 1417
Fragments arranged by size:
1417 1071 915 714 262
SnaBI TAC'GTA
Cuts at: 957 957
Size: 4379
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
StyI C'CwwG_G
Cuts at: 107 620 1513 1867 107
Size: 513 893 354 2619
Fragments arranged by size:
2619 893 513 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 757 919 1012 1962 2095 2554 2763 757
Size: 162 93 950 133 459 209 2373
Fragments arranged by size:
2373 950 459 209 162 133 93
TatI w'GTAC_w
Cuts at: 3894 3894
Size: 4379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 627 659 3896 3928 627
Size: 32 3237 32 1078
Fragments arranged by size:
3237 1078 32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 278 1874 278
Size: 1596 2783
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PciI PflMI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI PacI PasI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SrfI SspI StuI SwaI XhoI XmnI ZraI