Restriction map for
pF1KB3947
(Circular) (Six-base) MAPSORT of: pF1KB3947.seq Check: 3190 from: 1 to: 5777
pF1KB3947 5777 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1215 1452 1998 2815 1215
Size: 237 546 817 4177
Fragments arranged by size:
4177 817 546 237
Acc65I G'GTAC_C
Cuts at: 652 2793 652
Size: 2141 3636
AclI AA'CG_TT
Cuts at: 5617 5617
Size: 5777
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 3602 4034 4387 5049 1964
Size: 1638 432 353 662 2692
Fragments arranged by size:
2692 1638 662 432 353
AflIII A'CryG_T
Cuts at: 212 3129 4929 5210 212
Size: 2917 1800 281 779
Fragments arranged by size:
2917 1800 779 281
AhdI GACnn_n'nnGTC
Cuts at: 2692 2692
Size: 5777
AlwNI CAG_nnn'CTG
Cuts at: 923 3082 4520 923
Size: 2159 1438 2180
Fragments arranged by size:
2180 2159 1438
ApaLI G'TGCA_C
Cuts at: 4615 4615
Size: 5777
ApoI r'AATT_y
Cuts at: 351 1486 1756 2313 2781 5130 5386 351
Size: 1135 270 557 468 2349 256 742
Fragments arranged by size:
2349 1135 742 557 468 270 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5777
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
AvaI C'yCGr_G
Cuts at: 776 824 2797 776
Size: 48 1973 3756
Fragments arranged by size:
3756 1973 48
BamHI G'GATC_C
Cuts at: 2802 2802
Size: 5777
BanI G'GyrC_C
Cuts at: 652 1840 2793 3207 3455 3490 652
Size: 1188 953 414 248 35 2939
Fragments arranged by size:
2939 1188 953 414 248 35
BanII G_rGCy'C
Cuts at: 2326 2791 3821 2326
Size: 465 1030 4282
Fragments arranged by size:
4282 1030 465
BbeI G_GCGC'C
Cuts at: 3211 3459 3211
Size: 248 5529
BbsI GAAGACnn'nnnn_
Cuts at: 1885 2770 1885
Size: 885 4892
BbvCI CC'TCA_GC
Cuts at: 2546 2546
Size: 5777
BciVI GTATCCnnnnn_n'
Cuts at: 3675 4731 5777 3675
Size: 1056 1046 3675
Fragments arranged by size:
3675 1056 1046
BclI T'GATC_A
Cuts at: 1052 3297 1052
Size: 2245 3532
BfrBI ATG'CAT
Cuts at: 132 317 132
Size: 185 5592
BglII A'GATC_T
Cuts at: 233 3292 5329 233
Size: 3059 2037 681
Fragments arranged by size:
3059 2037 681
BlpI GC'TnA_GC
Cuts at: 811 1526 2889 811
Size: 715 1363 3699
Fragments arranged by size:
3699 1363 715
Bme1580I G_kGCm'C
Cuts at: 3402 3495 4619 3402
Size: 93 1124 4560
Fragments arranged by size:
4560 1124 93
BmgBI CAC'GTC
Cuts at: 1219 1996 1219
Size: 777 5000
BmrI ACTGGGnnnn_n'
Cuts at: 1876 2989 3070 3405 5549 1876
Size: 1113 81 335 2144 2104
Fragments arranged by size:
2144 2104 1113 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 5777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 1618
Size: 5777
Bpu10I CC'TnA_GC
Cuts at: 2546 2546
Size: 5777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 2953 3832 4546 4844 396
Size: 2557 879 714 298 1329
Fragments arranged by size:
2557 1329 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5481 5481
Size: 5777
BsaAI yAC'GTr
Cuts at: 1900 3130 1900
Size: 1230 4547
BsaBI GATnn'nnATC
Cuts at: 6 3291 5328 6
Size: 3285 2037 455
Fragments arranged by size:
3285 2037 455
BsaHI Gr'CG_yC
Cuts at: 3208 3456 4158 5654 3208
Size: 248 702 1496 3331
Fragments arranged by size:
3331 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2966 3487 4165 4576 4723 4997 2966
Size: 521 678 411 147 274 3746
Fragments arranged by size:
3746 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2726 2756 2726
Size: 30 5747
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 932 1356 932
Size: 424 5353
BseYI C'CCAG_C
Cuts at: 744 1536 2335 2650 3202 3960 4625 5033 744
Size: 792 799 315 552 758 665 408 1488
Fragments arranged by size:
1488 799 792 758 665 552 408 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2475 2475
Size: 5777
BsiEI CG_ry'CG
Cuts at: 104 3319 3365 4595 104
Size: 3215 46 1230 1286
Fragments arranged by size:
3215 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2791 3569 3759 4619 2791
Size: 778 190 860 3949
Fragments arranged by size:
3949 860 778 190
BsmI GAATG_Cn'
Cuts at: 134 1473 134
Size: 1339 4438
Bsp1286I G_dGCh'C
Cuts at: 2326 2791 3402 3495 3569 3759 3821 4619 2326
Size: 465 611 93 74 190 62 798 3484
Fragments arranged by size:
3484 798 611 465 190 93 74 62
BspHI T'CATG_A
Cuts at: 731 1569 4209 731
Size: 838 2640 2299
Fragments arranged by size:
2640 2299 838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2193 2827 3041 3343 3724 2193
Size: 634 214 302 381 4246
Fragments arranged by size:
4246 634 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4069 4123 5607 5772 50
Size: 4019 54 1484 165 55
Fragments arranged by size:
4019 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2390 3689 2390
Size: 1299 4478
BsrFI r'CCGG_y
Cuts at: 3775 3956 3775
Size: 181 5596
BssSI C'ACGA_G
Cuts at: 581 1874 2122 4048 4756 581
Size: 1293 248 1926 708 1602
Fragments arranged by size:
1926 1602 1293 708 248
BstAPI GCAn_nnn'nTGC
Cuts at: 535 3154 535
Size: 2619 3158
BstBI TT'CG_AA
Cuts at: 126 4138 126
Size: 4012 1765
BstYI r'GATC_y
Cuts at: 1 233 2802 3269 3292 3627 3873 4179 4191
Size: 232 2569 467 23 335 246 306 12
Cuts at: 4191 4277 5329 1
Size: 86 1052 449
Fragments arranged by size:
2569 1052 467 449 335 306 246 232
86 23 12
BtgI C'CryG_G
Cuts at: 2069 2330 2069
Size: 261 5516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3727 3908 4071 3727
Size: 181 163 5433
Fragments arranged by size:
5433 181 163
BtsI GCAGTG_nn'
Cuts at: 291 1961 3020 291
Size: 1670 1059 3048
Fragments arranged by size:
3048 1670 1059
DraI TTT'AAA
Cuts at: 2776 2776
Size: 5777
DrdI GACnn_nn'nnGTC
Cuts at: 3483 4827 3483
Size: 1344 4433
EaeI y'GGCC_r
Cuts at: 1796 3362 3536 3927 3954 5299 1796
Size: 1566 174 391 27 1345 2274
Fragments arranged by size:
2274 1566 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3362 3362
Size: 5777
EarI CTCTTCn'nnn_
Cuts at: 263 1262 2205 3800 4010 263
Size: 999 943 1595 210 2030
Fragments arranged by size:
2030 1595 999 943 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3985 4727 4873 5587 3985
Size: 742 146 714 4175
Fragments arranged by size:
4175 742 714 146
EcoICRI GAG'CTC
Cuts at: 2789 2789
Size: 5777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 1964 3602 4034 4387 5049 1618
Size: 346 1638 432 353 662 2346
Fragments arranged by size:
2346 1638 662 432 353 346
EcoO109I rG'GnC_Cy
Cuts at: 601 1732 2916 601
Size: 1131 1184 3462
Fragments arranged by size:
3462 1184 1131
EcoRI G'AATT_C
Cuts at: 2781 2781
Size: 5777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1134 1807 3796 1134
Size: 673 1989 3115
Fragments arranged by size:
3115 1989 673
FspI TGC'GCA
Cuts at: 3558 3558
Size: 5777
HaeII r_GCGC'y
Cuts at: 3211 3459 4689 5460 3211
Size: 248 1230 771 3528
Fragments arranged by size:
3528 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 905 918 950 1126 1158 1369 1401 1551
Size: 32 13 32 176 32 211 32 150
Cuts at: 1551 1583 2678 2710 3619 3649 3651 3681 873
Size: 32 1095 32 909 30 2 30 2969
Fragments arranged by size:
2969 1095 909 211 176 150 32 32
32 32 32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 361 2301 2816 361
Size: 1940 515 3322
Fragments arranged by size:
3322 1940 515
HindIII A'AGCT_T
Cuts at: 476 1612 476
Size: 1136 4641
KasI G'GCGC_C
Cuts at: 3207 3455 3207
Size: 248 5529
KpnI G_GTAC'C
Cuts at: 656 2797 656
Size: 2141 3636
MfeI C'AATT_G
Cuts at: 1206 1206
Size: 5777
MluI A'CGCG_T
Cuts at: 5210 5210
Size: 5777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 223 572 1529 1701 4536 4720 223
Size: 349 957 172 2835 184 1280
Fragments arranged by size:
2835 1280 957 349 184 172
MscI TGG'CCA
Cuts at: 1798 3538 1798
Size: 1740 4037
MslI CAynn'nnrTG
Cuts at: 196 211 1574 2074 3766 3893 196
Size: 15 1363 500 1692 127 2080
Fragments arranged by size:
2080 1692 1363 500 127 15
MspA1I CmG'CkG
Cuts at: 2489 2888 3202 3562 4346 4591 2489
Size: 399 314 360 784 245 3675
Fragments arranged by size:
3675 784 399 360 314 245
NaeI GCC'GGC
Cuts at: 3958 3958
Size: 5777
NarI GG'CG_CC
Cuts at: 3208 3456 3208
Size: 248 5529
NcoI C'CATG_G
Cuts at: 2069 2330 2069
Size: 261 5516
NgoMIV G'CCGG_C
Cuts at: 3956 3956
Size: 5777
NsiI A_TGCA'T
Cuts at: 134 319 134
Size: 185 5592
NspI r_CATG'y
Cuts at: 216 705 2588 2830 3330 4933 216
Size: 489 1883 242 500 1603 1060
Fragments arranged by size:
1883 1603 1060 500 489 242
PasI CC'CwG_GG
Cuts at: 158 1424 158
Size: 1266 4511
PciI A'CATG_T
Cuts at: 212 4929 212
Size: 4717 1060
PmeI GTTT'AAAC
Cuts at: 2776 2776
Size: 5777
PmlI CAC'GTG
Cuts at: 1900 1900
Size: 5777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 4223
Size: 32 5745
PpuMI rG'GwC_Cy
Cuts at: 601 1732 601
Size: 1131 4646
PsiI TTA'TAA
Cuts at: 295 1306 1658 295
Size: 1011 352 4414
Fragments arranged by size:
4414 1011 352
PstI C_TGCA'G
Cuts at: 1819 1917 2824 3509 1819
Size: 98 907 685 4087
Fragments arranged by size:
4087 907 685 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5777
PvuII CAG'CTG
Cuts at: 2489 3202 3562 2489
Size: 713 360 4704
Fragments arranged by size:
4704 713 360
RsrII CG'GwC_CG
Cuts at: 3972 3972
Size: 5777
SacI G_AGCT'C
Cuts at: 2791 2791
Size: 5777
SalI G'TCGA_C
Cuts at: 2814 2814
Size: 5777
SapI GCTCTTCn'nnn_
Cuts at: 3800 4010 3800
Size: 210 5567
SbfI CC_TGCA'GG
Cuts at: 2824 2824
Size: 5777
ScaI AGT'ACT
Cuts at: 1391 5294 1391
Size: 3903 1874
SfcI C'TryA_G
Cuts at: 33 1178 1453 1815 1913 1999 2504 2820 3505
Size: 1145 275 362 98 86 505 316 685
Cuts at: 3505 4473 4664 33
Size: 968 191 1146
Fragments arranged by size:
1146 1145 968 685 505 362 316 275
191 98 86
SfoI GGC'GCC
Cuts at: 3209 3457 3209
Size: 248 5529
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
SmaI CCC'GGG
Cuts at: 2799 2799
Size: 5777
SmlI C'TyrA_G
Cuts at: 411 2932 3847 4561 4823 411
Size: 2521 915 714 262 1365
Fragments arranged by size:
2521 1365 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5777
SphI G_CATG'C
Cuts at: 2588 2830 3330 2588
Size: 242 500 5035
Fragments arranged by size:
5035 500 242
SspI AAT'ATT
Cuts at: 576 576
Size: 5777
StyI C'CwwG_G
Cuts at: 758 1667 2069 2249 2330 2745 2911 3265 758
Size: 909 402 180 81 415 166 354 3270
Fragments arranged by size:
3270 909 415 402 354 180 166 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2512 3360 3493 3952 4161 2512
Size: 848 133 459 209 4128
Fragments arranged by size:
4128 848 459 209 133
TatI w'GTAC_w
Cuts at: 427 952 1112 1389 2170 5292 427
Size: 525 160 277 781 3122 912
Fragments arranged by size:
3122 912 781 525 277 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1860 1892 5294 5326 1860
Size: 32 3402 32 2311
Fragments arranged by size:
3402 2311 32 32
Tth111I GACn'n_nGTC
Cuts at: 3574 3574
Size: 5777
XbaI T'CTAG_A
Cuts at: 2808 2808
Size: 5777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3272 3272
Size: 5777
XmaI C'CCGG_G
Cuts at: 2797 2797
Size: 5777
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PmeI
PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmtI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI PacI PflMI PfoI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI