Restriction map for
pF1KB3951
(Circular) (Six-base) MAPSORT of: pF1KB3951.seq Check: 5939 from: 1 to: 4028
pF1KB3951 4028 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 356 456 1066 356
Size: 100 610 3318
Fragments arranged by size:
3318 610 100
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 725 1853 2285 3032 3300 199
Size: 526 1128 432 747 268 927
Fragments arranged by size:
1128 927 747 526 432 268
AflIII A'CryG_T
Cuts at: 984 1380 2484 3461 984
Size: 396 1104 977 1551
Fragments arranged by size:
1551 1104 977 396
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 178 178
Size: 4028
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 302 334 302
Size: 32 3996
AlwNI CAG_nnn'CTG
Cuts at: 703 1333 2900 703
Size: 630 1567 1831
Fragments arranged by size:
1831 1567 630
ApaLI G'TGCA_C
Cuts at: 826 2798 826
Size: 1972 2056
ApoI r'AATT_y
Cuts at: 632 659 841 1032 3381 3637 632
Size: 27 182 191 2349 256 1023
Fragments arranged by size:
2349 1023 256 191 182 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 734 1048 734
Size: 314 3714
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 111 1044 1458 1706 1741 111
Size: 933 414 248 35 2398
Fragments arranged by size:
2398 933 414 248 35
BanII G_rGCy'C
Cuts at: 1042 2072 1042
Size: 1030 2998
BbeI G_GCGC'C
Cuts at: 115 1462 1710 115
Size: 1347 248 2433
Fragments arranged by size:
2433 1347 248
BbsI GAAGACnn'nnnn_
Cuts at: 918 918
Size: 4028
BciVI GTATCCnnnnn_n'
Cuts at: 708 1926 2687 4028 708
Size: 1218 761 1341 708
Fragments arranged by size:
1341 1218 761 708
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 830 1653 1746 2802 830
Size: 823 93 1056 2056
Fragments arranged by size:
2056 1056 823 93
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 543 329
Size: 214 3814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2083 2575 2873 1204
Size: 879 492 298 2359
Fragments arranged by size:
2359 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 373 3732 373
Size: 3359 669
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 112 1459 1707 2409 3905 112
Size: 1347 248 702 1496 235
Fragments arranged by size:
1496 1347 702 248 235
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 302 332 516 546 749 779 302
Size: 30 184 30 203 30 3551
Fragments arranged by size:
3551 203 184 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 752 199
Size: 553 3475
BseYI C'CCAG_C
Cuts at: 1453 2211 2788 3284 1453
Size: 758 577 496 2197
Fragments arranged by size:
2197 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 816 830 1042 1820 2010 2802 816
Size: 14 212 778 190 792 2042
Fragments arranged by size:
2042 792 778 212 190 14
Bsp1286I G_dGCh'C
Cuts at: 816 830 1042 1653 1746 1820 2010 2072 2802
Size: 14 212 611 93 74 190 62 730
Cuts at: 2802 816
Size: 2042
Fragments arranged by size:
2042 730 611 212 190 93 74 62
14
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 447 3204 447
Size: 2757 1271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 733 1078 1292 1594 1975 170
Size: 563 345 214 302 381 2223
Fragments arranged by size:
2223 563 381 345 302 214
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 636 2389 636
Size: 1753 2275
BstYI r'GATC_y
Cuts at: 1 699 1053 1520 1543 1878 2124 3136 3222
Size: 698 354 467 23 335 246 1012 86
Cuts at: 3222 3234 3580 1
Size: 12 346 449
Fragments arranged by size:
1012 698 467 449 354 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 270 594 107
Size: 163 324 3541
Fragments arranged by size:
3541 324 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 873 1734 2592 873
Size: 861 858 2309
Fragments arranged by size:
2309 861 858
EaeI y'GGCC_r
Cuts at: 1613 1787 2178 2205 3550 1613
Size: 174 391 27 1345 2091
Fragments arranged by size:
2091 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 212 2051 2261 212
Size: 1839 210 1979
Fragments arranged by size:
1979 1839 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 329 543 725 1853 2285 3032 3300 199
Size: 130 214 182 1128 432 747 268 927
Fragments arranged by size:
1128 927 747 432 268 214 182 130
EcoNI CCTnn'n_nnAGG
Cuts at: 668 668
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
EcoRV GAT'ATC
Cuts at: 267 615 267
Size: 348 3680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 115 1462 1710 2732 3711 115
Size: 1347 248 1022 979 432
Fragments arranged by size:
1347 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 749 781 1870 1900 1902 1932 749
Size: 32 1089 30 2 30 2845
Fragments arranged by size:
2845 1089 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 111 1458 1706 111
Size: 1347 248 2433
Fragments arranged by size:
2433 1347 248
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2883 2699
Size: 184 3844
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 340 2017 2144 340
Size: 1677 127 2224
Fragments arranged by size:
2224 1677 127
MspA1I CmG'CkG
Cuts at: 1139 1453 1813 2826 3071 1139
Size: 314 360 1013 245 2096
Fragments arranged by size:
2096 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 112 1459 1707 112
Size: 1347 248 2433
Fragments arranged by size:
2433 1347 248
NcoI C'CATG_G
Cuts at: 107 270 594 107
Size: 163 324 3541
Fragments arranged by size:
3541 324 163
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 552 1081 1581 2488 552
Size: 529 500 907 2092
Fragments arranged by size:
2092 907 529 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 302 334 3167 3199 302
Size: 32 2833 32 1131
Fragments arranged by size:
2833 1131 32 32
PstI C_TGCA'G
Cuts at: 811 1075 1760 811
Size: 264 685 3079
Fragments arranged by size:
3079 685 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 369 807 1071 1756 2749 2940 33
Size: 336 438 264 685 993 191 1121
Fragments arranged by size:
1121 993 685 438 336 264 191
SfoI GGC'GCC
Cuts at: 113 1460 1708 113
Size: 1347 248 2433
Fragments arranged by size:
2433 1347 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 1183 2098 2590 2852 1183
Size: 915 492 262 2359
Fragments arranged by size:
2359 915 492 262
SpeI A'CTAG_T
Cuts at: 67 822 67
Size: 755 3273
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
SspI AAT'ATT
Cuts at: 909 909
Size: 4028
StyI C'CwwG_G
Cuts at: 107 270 594 1162 1516 107
Size: 163 324 568 354 2619
Fragments arranged by size:
2619 568 354 324 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 1611 1744 2203 2412 334
Size: 1277 133 459 209 1950
Fragments arranged by size:
1950 1277 459 209 133
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 502 1523 502
Size: 1021 3007
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI