Restriction map for
pF1KB3954
(Circular) (Six-base) MAPSORT of: pF1KB3954.seq Check: 4610 from: 1 to: 7511
pF1KB3954 7511 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1048 1390 1048
Size: 342 7169
AccI GT'mk_AC
Cuts at: 810 1791 4549 810
Size: 981 2758 3772
Fragments arranged by size:
3772 2758 981
Acc65I G'GTAC_C
Cuts at: 4527 4527
Size: 7511
AclI AA'CG_TT
Cuts at: 7351 7351
Size: 7511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 4166 4297 4334 5336 5768 6515 6783 725
Size: 3441 131 37 1002 432 747 268 1453
Fragments arranged by size:
3441 1453 1002 747 432 268 131 37
AflIII A'CryG_T
Cuts at: 4080 4863 5967 6944 4080
Size: 783 1104 977 4647
Fragments arranged by size:
4647 1104 977 783
AgeI A'CCGG_T
Cuts at: 5899 5899
Size: 7511
AleI CACnn'nnGTG
Cuts at: 1238 1238
Size: 7511
AlwNI CAG_nnn'CTG
Cuts at: 1549 1648 2359 2530 2827 3475 3511 4816 6383
Size: 99 711 171 297 648 36 1305 1567
Cuts at: 6383 1549
Size: 2677
Fragments arranged by size:
2677 1567 1305 711 648 297 171 99
36
ApaI G_GGCC'C
Cuts at: 1658 2181 2819 2926 1658
Size: 523 638 107 6243
Fragments arranged by size:
6243 638 523 107
ApaLI G'TGCA_C
Cuts at: 2105 6281 2105
Size: 4176 3335
ApoI r'AATT_y
Cuts at: 341 3904 4303 4327 4471 4515 6864 7120 341
Size: 3563 399 24 144 44 2349 256 732
Fragments arranged by size:
3563 2349 732 399 256 144 44 24
AseI AT'TA_AT
Cuts at: 20 1271 20
Size: 1251 6260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7511
AvaI C'yCGr_G
Cuts at: 1064 1082 1343 1820 3350 3602 4531 1064
Size: 18 261 477 1530 252 929 4044
Fragments arranged by size:
4044 1530 929 477 261 252 18
AvrII C'CTAG_G
Cuts at: 3007 3007
Size: 7511
BamHI G'GATC_C
Cuts at: 280 1141 4536 280
Size: 861 3395 3255
Fragments arranged by size:
3395 3255 861
BanI G'GyrC_C
Cuts at: 1195 1420 1564 2140 2455 2914 3229 3454 3679
Size: 225 144 576 315 459 315 225 225
Cuts at: 3679 4527 4941 5189 5224 1195
Size: 848 414 248 35 3482
Fragments arranged by size:
3482 848 576 459 414 315 315 248
225 225 225 144 35
BanII G_rGCy'C
Cuts at: 993 1020 1326 1344 1436 1658 2118 2181 2595
Size: 27 306 18 92 222 460 63 414
Cuts at: 2595 2819 2926 2937 3637 4525 5555 993
Size: 224 107 11 700 888 1030 2949
Fragments arranged by size:
2949 1030 888 700 460 414 306 224
222 107 92 63 27 18 11
BbeI G_GCGC'C
Cuts at: 4945 5193 4945
Size: 248 7263
BbsI GAAGACnn'nnnn_
Cuts at: 964 2737 3097 3160 964
Size: 1773 360 63 5315
Fragments arranged by size:
5315 1773 360 63
BciVI GTATCCnnnnn_n'
Cuts at: 694 866 5409 6170 7511 694
Size: 172 4543 761 1341 694
Fragments arranged by size:
4543 1341 761 694 172
BclI T'GATC_A
Cuts at: 4464 5031 4464
Size: 567 6944
BfrBI ATG'CAT
Cuts at: 4013 4013
Size: 7511
BglI GCCn_nnn'nGGC
Cuts at: 644 1977 2426 3317 644
Size: 1333 449 891 4838
Fragments arranged by size:
4838 1333 891 449
BglII A'GATC_T
Cuts at: 5026 7063 5026
Size: 2037 5474
BlpI GC'TnA_GC
Cuts at: 4623 4623
Size: 7511
Bme1580I G_kGCm'C
Cuts at: 1200 1569 1658 2109 2145 2181 2460 2819 2919
Size: 369 89 451 36 36 279 359 100
Cuts at: 2919 2926 3234 3459 3684 5136 5229 6285 1200
Size: 7 308 225 225 1452 93 1056 2426
Fragments arranged by size:
2426 1452 1056 451 369 359 308 279
225 225 100 93 89 36 36 7
BmrI ACTGGGnnnn_n'
Cuts at: 561 1985 3533 3948 4010 4379 4723 4804 5139
Size: 1424 1548 415 62 369 344 81 335
Cuts at: 5139 7283 561
Size: 2144 789
Fragments arranged by size:
2144 1548 1424 789 415 369 344 335
81 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5878 5878
Size: 7511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 680 734 833 845 1337 1340 1538 1625
Size: 66 54 99 12 492 3 198 87
Cuts at: 1625 1646 1691 1718 1925 2039 2102 2132 2312
Size: 21 45 27 207 114 63 30 180
Cuts at: 2312 2456 2507 2645 2663 2786 2879 2921 2987
Size: 144 51 138 18 123 93 42 66
Cuts at: 2987 3452 4173 4366 614
Size: 465 721 193 3759
Fragments arranged by size:
3759 721 492 465 207 198 193 180
144 138 123 114 99 93 87 66
66 63 54 51 45 42 30 27
21 18 12 3
Bpu10I CC'TnA_GC
Cuts at: 1154 1154
Size: 7511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 1229 3353 3805 3907 4687 5566 6058 6356
Size: 825 2124 452 102 780 879 492 298
Cuts at: 6356 404
Size: 1559
Fragments arranged by size:
2124 1559 879 825 780 492 452 298
102
BsaI GGTCTCn'nnnn_
Cuts at: 1184 3073 3890 7215 1184
Size: 1889 817 3325 1480
Fragments arranged by size:
3325 1889 1480 817
BsaAI yAC'GTr
Cuts at: 1444 3379 4864 1444
Size: 1935 1485 4091
Fragments arranged by size:
4091 1935 1485
BsaBI GATnn'nnATC
Cuts at: 6 5025 7062 6
Size: 5019 2037 455
Fragments arranged by size:
5019 2037 455
BsaHI Gr'CG_yC
Cuts at: 1691 4942 5190 5892 7388 1691
Size: 3251 248 702 1496 1814
Fragments arranged by size:
3251 1814 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4700 5221 5899 6173 6320 6731 4700
Size: 521 678 274 147 411 5480
Fragments arranged by size:
5480 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 384 414 453 483 587 617 930 960 2505
Size: 30 39 30 104 30 313 30 1545
Cuts at: 2505 2535 2829 2859 384
Size: 30 294 30 5036
Fragments arranged by size:
5036 1545 313 294 104 39 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1226 1676 2315 3314 1226
Size: 450 639 999 5423
Fragments arranged by size:
5423 999 639 450
BseYI C'CCAG_C
Cuts at: 868 2421 2791 2956 3724 4936 5694 6271 6767
Size: 1553 370 165 768 1212 758 577 496
Cuts at: 6767 868
Size: 1612
Fragments arranged by size:
1612 1553 1212 768 758 577 496 370
165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1418 4183 383
Size: 1035 2765 3711
Fragments arranged by size:
3711 2765 1035
BsiEI CG_ry'CG
Cuts at: 104 5053 5099 6307 104
Size: 4949 46 1208 1308
Fragments arranged by size:
4949 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 948 993 1065 1083 1245 1326 1380 1641 1875
Size: 45 72 18 162 81 54 261 234
Cuts at: 1875 2109 2118 2550 2739 2847 2937 2973 3333
Size: 234 9 432 189 108 90 36 360
Cuts at: 3333 3360 4525 5303 5493 6285 948
Size: 27 1165 778 190 792 2174
Fragments arranged by size:
2174 1165 792 778 432 360 261 234
234 190 189 162 108 90 81 72
54 45 36 27 18 9
BsmI GAATG_Cn'
Cuts at: 1313 2291 4171 1313
Size: 978 1880 4653
Fragments arranged by size:
4653 1880 978
Bsp1286I G_dGCh'C
Cuts at: 948 993 1020 1065 1083 1200 1245 1326 1344
Size: 45 27 45 18 117 45 81 18
Cuts at: 1344 1380 1436 1569 1641 1658 1875 2109 2118
Size: 36 56 133 72 17 217 234 9
Cuts at: 2118 2145 2181 2460 2550 2595 2739 2819 2847
Size: 27 36 279 90 45 144 80 28
Cuts at: 2847 2919 2926 2937 2973 3234 3333 3360 3459
Size: 72 7 11 36 261 99 27 99
Cuts at: 3459 3637 3684 4525 5136 5229 5303 5493 5555
Size: 178 47 841 611 93 74 190 62
Cuts at: 5555 6285 948
Size: 730 2174
Fragments arranged by size:
2174 841 730 611 279 261 234 217
190 178 144 133 117 99 99 93
90 81 80 74 72 72 62 56
47 45 45 45 45 36 36 36
28 27 27 27 18 18 17 11
9 7
BspEI T'CCGG_A
Cuts at: 6731 6731
Size: 7511
BspHI T'CATG_A
Cuts at: 6687 6687
Size: 7511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1179 1390 1593 2538 2664 3519 4561 4775
Size: 131 211 203 945 126 855 1042 214
Cuts at: 4775 5077 5458 1048
Size: 302 381 3101
Fragments arranged by size:
3101 1042 945 855 381 302 214 211
203 131 126
BsrBI CCG'CTC
Cuts at: 50 5803 5857 7341 7506 50
Size: 5753 54 1484 165 55
Fragments arranged by size:
5753 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3719 4232 5423 3719
Size: 513 1191 5807
Fragments arranged by size:
5807 1191 513
BsrFI r'CCGG_y
Cuts at: 1363 2101 5509 5690 5899 1363
Size: 738 3408 181 209 2975
Fragments arranged by size:
3408 2975 738 209 181
BssSI C'ACGA_G
Cuts at: 2486 2738 5782 6140 2486
Size: 252 3044 358 3857
Fragments arranged by size:
3857 3044 358 252
BstAPI GCAn_nnn'nTGC
Cuts at: 2286 4888 2286
Size: 2602 4909
BstBI TT'CG_AA
Cuts at: 5872 5872
Size: 7511
BstEII G'GTnAC_C
Cuts at: 469 2770 469
Size: 2301 5210
BstYI r'GATC_y
Cuts at: 1 280 435 691 1141 3184 3625 4536 5003
Size: 279 155 256 450 2043 441 911 467
Cuts at: 5003 5026 5361 5607 6619 6705 6717 7063 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2043 1012 911 467 450 449 441 346
335 279 256 246 155 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1226 1919 1955 3026 1226
Size: 693 36 1071 5711
Fragments arranged by size:
5711 1071 693 36
BtgI C'CryG_G
Cuts at: 1001 4039 4100 1001
Size: 3038 61 4412
Fragments arranged by size:
4412 3038 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 308 5461 5642 5805 308
Size: 5153 181 163 2014
Fragments arranged by size:
5153 2014 181 163
BtsI GCAGTG_nn'
Cuts at: 1095 4218 4754 1095
Size: 3123 536 3852
Fragments arranged by size:
3852 3123 536
DraI TTT'AAA
Cuts at: 4510 4510
Size: 7511
DraIII CAC_nnn'GTG
Cuts at: 1447 2257 3382 1447
Size: 810 1125 5576
Fragments arranged by size:
5576 1125 810
DrdI GACnn_nn'nnGTC
Cuts at: 1004 2012 2660 2786 3101 3578 5217 6075 1004
Size: 1008 648 126 315 477 1639 858 2440
Fragments arranged by size:
2440 1639 1008 858 648 477 315 126
EaeI y'GGCC_r
Cuts at: 1036 2427 2886 5096 5270 5661 5688 7033 1036
Size: 1391 459 2210 174 391 27 1345 1514
Fragments arranged by size:
2210 1514 1391 1345 459 391 174 27
EagI C'GGCC_G
Cuts at: 5096 5096
Size: 7511
EarI CTCTTCn'nnn_
Cuts at: 3135 5534 5744 3135
Size: 2399 210 4902
Fragments arranged by size:
4902 2399 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5719 6029 6175 7321 5719
Size: 310 146 1146 5909
Fragments arranged by size:
5909 1146 310 146
EcoICRI GAG'CTC
Cuts at: 991 1324 2116 2935 4523 991
Size: 333 792 819 1588 3979
Fragments arranged by size:
3979 1588 819 792 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 680 725 734 833 845 1337 1340 1538
Size: 66 45 9 99 12 492 3 198
Cuts at: 1538 1625 1646 1691 1718 1925 2039 2102 2132
Size: 87 21 45 27 207 114 63 30
Cuts at: 2132 2312 2456 2507 2645 2663 2786 2879 2921
Size: 180 144 51 138 18 123 93 42
Cuts at: 2921 2987 3452 4166 4173 4297 4334 4366 5336
Size: 66 465 714 7 124 37 32 970
Cuts at: 5336 5768 6515 6783 614
Size: 432 747 268 1342
Fragments arranged by size:
1342 970 747 714 492 465 432 268
207 198 180 144 138 124 123 114
99 93 87 66 66 63 51 45
45 42 37 32 30 27 21 18
12 9 7 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 3953 2042
Size: 1911 5600
EcoO109I rG'GnC_Cy
Cuts at: 415 424 442 497 646 709 745 754 1105
Size: 9 18 55 149 63 36 9 351
Cuts at: 1105 1249 1303 1348 1582 1618 1645 1654 1762
Size: 144 54 45 234 36 27 9 108
Cuts at: 1762 1798 1909 1924 1960 1969 2005 2177 2178
Size: 36 111 15 36 9 36 172 1
Cuts at: 2178 2392 2527 2617 2635 2779 2788 2816 2824
Size: 214 135 90 18 144 9 28 8
Cuts at: 2824 2922 3004 3472 3508 3517 3526 3580 3598
Size: 98 82 468 36 9 9 54 18
Cuts at: 3598 3661 4272 4650 415
Size: 63 611 378 3276
Fragments arranged by size:
3276 611 468 378 351 234 214 172
149 144 144 135 111 108 98 90
82 63 63 55 54 54 45 36
36 36 36 36 36 28 27 18
18 18 15 9 9 9 9 9
9 9 8 1
EcoRI G'AATT_C
Cuts at: 4303 4515 4303
Size: 212 7299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4171 5530 4171
Size: 1359 6152
FspI TGC'GCA
Cuts at: 5292 5292
Size: 7511
HaeII r_GCGC'y
Cuts at: 3242 4945 5193 6215 7194 3242
Size: 1703 248 1022 979 3559
Fragments arranged by size:
3559 1703 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 272 297 304 574 606 787 819 1676
Size: 7 25 7 270 32 181 32 857
Cuts at: 1676 1708 2890 2922 5353 5383 5385 5415 265
Size: 32 1182 32 2431 30 2 30 2361
Fragments arranged by size:
2431 2361 1182 857 270 181 32 32
32 32 30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 1099 1792 3945 4550 1099
Size: 693 2153 605 4060
Fragments arranged by size:
4060 2153 693 605
KasI G'GCGC_C
Cuts at: 4941 5189 4941
Size: 248 7263
KpnI G_GTAC'C
Cuts at: 4531 4531
Size: 7511
MluI A'CGCG_T
Cuts at: 6944 6944
Size: 7511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 406 1146 3513 3687 6182 6366 406
Size: 740 2367 174 2495 184 1551
Fragments arranged by size:
2495 2367 1551 740 184 174
MscI TGG'CCA
Cuts at: 2429 2888 5272 2429
Size: 459 2384 4668
Fragments arranged by size:
4668 2384 459
MslI CAynn'nnrTG
Cuts at: 1238 5500 5627 1238
Size: 4262 127 3122
Fragments arranged by size:
4262 3122 127
MspA1I CmG'CkG
Cuts at: 634 868 1309 2425 2795 3127 3280 3715 4167
Size: 234 441 1116 370 332 153 435 452
Cuts at: 4167 4622 4936 5296 6309 6554 634
Size: 455 314 360 1013 245 1591
Fragments arranged by size:
1591 1116 1013 455 452 441 435 370
360 332 314 245 234 153
NaeI GCC'GGC
Cuts at: 5692 5692
Size: 7511
NarI GG'CG_CC
Cuts at: 4942 5190 4942
Size: 248 7263
NcoI C'CATG_G
Cuts at: 1001 4100 1001
Size: 3099 4412
NdeI CA'TA_TG
Cuts at: 579 579
Size: 7511
NgoMIV G'CCGG_C
Cuts at: 5690 5690
Size: 7511
NsiI A_TGCA'T
Cuts at: 4015 4015
Size: 7511
NspI r_CATG'y
Cuts at: 1722 3000 4013 4564 5064 5971 1722
Size: 1278 1013 551 500 907 3262
Fragments arranged by size:
3262 1278 1013 907 551 500
PasI CC'CwG_GG
Cuts at: 1570 1966 2371 2919 3099 3649 1570
Size: 396 405 548 180 550 5432
Fragments arranged by size:
5432 550 548 405 396 180
PciI A'CATG_T
Cuts at: 5967 5967
Size: 7511
PfoI T'CCnGG_A
Cuts at: 483 624 696 750 948 993 1119 1317 1326
Size: 141 72 54 198 45 126 198 9
Cuts at: 1326 1353 1380 1479 1542 1704 1731 1767 1929
Size: 27 27 99 63 162 27 36 162
Cuts at: 1929 2055 2118 2163 2523 2550 2622 3504 3666
Size: 126 63 45 360 27 72 882 162
Cuts at: 3666 483
Size: 4328
Fragments arranged by size:
4328 882 360 198 198 162 162 162
141 126 126 99 72 72 63 63
54 45 45 36 27 27 27 27
9
PmeI GTTT'AAAC
Cuts at: 4510 4510
Size: 7511
PmlI CAC'GTG
Cuts at: 1444 3379 1444
Size: 1935 5576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6650 6682 6650
Size: 32 7479
PpuMI rG'GwC_Cy
Cuts at: 415 497 709 754 1249 1303 1348 1582 1618
Size: 82 212 45 495 54 45 234 36
Cuts at: 1618 1798 1909 1924 1960 2005 2392 2527 2635
Size: 180 111 15 36 45 387 135 108
Cuts at: 2635 2779 2788 2824 3004 3508 3526 3580 415
Size: 144 9 36 180 504 18 54 4346
Fragments arranged by size:
4346 504 495 387 234 212 180 180
144 135 111 108 82 54 54 45
45 45 36 36 36 18 15 9
PspOMI G'GGCC_C
Cuts at: 1654 2177 2815 2922 1654
Size: 523 638 107 6243
Fragments arranged by size:
6243 638 523 107
PspXI vC'TCGA_Gb
Cuts at: 1082 3602 1082
Size: 2520 4991
PstI C_TGCA'G
Cuts at: 1059 1176 1554 1653 3516 3895 4558 5243 1059
Size: 117 378 99 1863 379 663 685 3327
Fragments arranged by size:
3327 1863 685 663 379 378 117 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7511
PvuII CAG'CTG
Cuts at: 634 868 2425 3127 3280 4167 4936 5296 634
Size: 234 1557 702 153 887 769 360 2849
Fragments arranged by size:
2849 1557 887 769 702 360 234 153
RsrII CG'GwC_CG
Cuts at: 5706 5706
Size: 7511
SacI G_AGCT'C
Cuts at: 993 1326 2118 2937 4525 993
Size: 333 792 819 1588 3979
Fragments arranged by size:
3979 1588 819 792 333
SalI G'TCGA_C
Cuts at: 1790 4548 1790
Size: 2758 4753
SanDI GG'GwC_CC
Cuts at: 497 1303 1960 497
Size: 806 657 6048
Fragments arranged by size:
6048 806 657
SapI GCTCTTCn'nnn_
Cuts at: 5534 5744 5534
Size: 210 7301
SbfI CC_TGCA'GG
Cuts at: 1176 1653 3516 4558 1176
Size: 477 1863 1042 4129
Fragments arranged by size:
4129 1863 1042 477
ScaI AGT'ACT
Cuts at: 7028 7028
Size: 7511
SexAI A'CCwGG_T
Cuts at: 1524 1794 2082 2253 2568 2712 3261 1524
Size: 270 288 171 315 144 549 5774
Fragments arranged by size:
5774 549 315 288 270 171 144
SfcI C'TryA_G
Cuts at: 33 767 1055 1172 1550 1649 3512 3891 4554
Size: 734 288 117 378 99 1863 379 663
Cuts at: 4554 5239 6232 6423 33
Size: 685 993 191 1121
Fragments arranged by size:
1863 1121 993 734 685 663 379 378
288 191 117 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 644 1977 2426 3317 644
Size: 1333 449 891 4838
Fragments arranged by size:
4838 1333 891 449
SfoI GGC'GCC
Cuts at: 4943 5191 4943
Size: 248 7263
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7511
SmaI CCC'GGG
Cuts at: 1345 4533 1345
Size: 3188 4323
SmlI C'TyrA_G
Cuts at: 419 1082 1244 3368 3602 3820 3922 4666 5581
Size: 663 162 2124 234 218 102 744 915
Cuts at: 5581 6073 6335 419
Size: 492 262 1595
Fragments arranged by size:
2124 1595 915 744 663 492 262 234
218 162 102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7511
SphI G_CATG'C
Cuts at: 1722 3000 4564 5064 1722
Size: 1278 1564 500 4169
Fragments arranged by size:
4169 1564 1278 500
StyI C'CwwG_G
Cuts at: 428 705 1001 2386 2676 3007 3954 4100 4645
Size: 277 296 1385 290 331 947 146 545
Cuts at: 4645 4999 428
Size: 354 2940
Fragments arranged by size:
2940 1385 947 545 354 331 296 290
277 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3262 3662 5094 5227 5686 5895 3262
Size: 400 1432 133 459 209 4878
Fragments arranged by size:
4878 1432 459 400 209 133
TatI w'GTAC_w
Cuts at: 7026 7026
Size: 7511
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 453 485 2829 2861 3720 3752 7028 7060 453
Size: 32 2344 32 859 32 3276 32 904
Fragments arranged by size:
3276 2344 904 859 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1796 5308 1796
Size: 3512 3999
XbaI T'CTAG_A
Cuts at: 4542 4542
Size: 7511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 712 3106 4046 5006 168
Size: 544 2394 940 960 2673
Fragments arranged by size:
2673 2394 960 940 544
XhoI C'TCGA_G
Cuts at: 1082 3602 1082
Size: 2520 4991
XmaI C'CCGG_G
Cuts at: 1343 4531 1343
Size: 3188 4323
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI BaeI
BbvCI BcgI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI PacI PflMI PshAI
PsiI PsrI SacII SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI