Restriction map for
pF1KB3955
(Circular) (Six-base) MAPSORT of: pF1KB3955.seq Check: 6318 from: 1 to: 4475
pF1KB3955 4475 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 732 732
Size: 4475
AccI GT'mk_AC
Cuts at: 1271 1513 1271
Size: 242 4233
Acc65I G'GTAC_C
Cuts at: 294 1491 294
Size: 1197 3278
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1163 1360 2300 2732 3085 3747 1027
Size: 136 197 940 432 353 662 1755
Fragments arranged by size:
1755 940 662 432 353 197 136
AflIII A'CryG_T
Cuts at: 1463 1827 3627 3908 1463
Size: 364 1800 281 2030
Fragments arranged by size:
2030 1800 364 281
AleI CACnn'nnGTG
Cuts at: 538 538
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 1780 3218 1780
Size: 1438 3037
ApaI G_GGCC'C
Cuts at: 931 931
Size: 4475
ApaLI G'TGCA_C
Cuts at: 3313 3313
Size: 4475
ApoI r'AATT_y
Cuts at: 205 436 970 1086 1123 1479 3828 4084 205
Size: 231 534 116 37 356 2349 256 596
Fragments arranged by size:
2349 596 534 356 256 231 116 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 1495 1495
Size: 4475
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 294 1491 1905 2153 2188 294
Size: 1197 414 248 35 2581
Fragments arranged by size:
2581 1197 414 248 35
BanII G_rGCy'C
Cuts at: 931 1489 2519 931
Size: 558 1030 2887
Fragments arranged by size:
2887 1030 558
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 1348 1348
Size: 4475
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3429 4475 2373
Size: 1056 1046 2373
Fragments arranged by size:
2373 1056 1046
BclI T'GATC_A
Cuts at: 480 1995 480
Size: 1515 2960
BfrBI ATG'CAT
Cuts at: 559 559
Size: 4475
BglII A'GATC_T
Cuts at: 219 252 1990 4027 219
Size: 33 1738 2037 667
Fragments arranged by size:
2037 1738 667 33
BlpI GC'TnA_GC
Cuts at: 1587 1587
Size: 4475
Bme1580I G_kGCm'C
Cuts at: 931 2100 2193 3317 931
Size: 1169 93 1124 2089
Fragments arranged by size:
2089 1169 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 802 1687 1768 2103 4247 802
Size: 885 81 335 2144 1030
Fragments arranged by size:
2144 1030 885 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1277
Size: 4475
Bpu10I CC'TnA_GC
Cuts at: 290 290
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 2530 3244 3542 1651
Size: 879 714 298 2584
Fragments arranged by size:
2584 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4179 4179
Size: 4475
BsaAI yAC'GTr
Cuts at: 1464 1828 1464
Size: 364 4111
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 729 1906 2154 2856 4352 729
Size: 1177 248 702 1496 852
Fragments arranged by size:
1496 1177 852 702 248
BsaWI w'CCGG_w
Cuts at: 1664 2185 2863 3274 3421 3695 1664
Size: 521 678 411 147 274 2444
Fragments arranged by size:
2444 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 156 186 156
Size: 30 4445
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 854 1384 854
Size: 530 3945
BseYI C'CCAG_C
Cuts at: 603 1900 2658 3323 3731 603
Size: 1297 758 665 408 1347
Fragments arranged by size:
1347 1297 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3293 104
Size: 1913 46 1230 1286
Fragments arranged by size:
1913 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1489 2267 2457 3317 1489
Size: 778 190 860 2647
Fragments arranged by size:
2647 860 778 190
BsmI GAATG_Cn'
Cuts at: 1166 1166
Size: 4475
Bsp1286I G_dGCh'C
Cuts at: 931 1489 2100 2193 2267 2457 2519 3317 931
Size: 558 611 93 74 190 62 798 2089
Fragments arranged by size:
2089 798 611 558 190 93 74 62
BspHI T'CATG_A
Cuts at: 228 2907 228
Size: 2679 1796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1525 1739 2041 2422 1525
Size: 214 302 381 3578
Fragments arranged by size:
3578 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2767 2821 4305 4470 50
Size: 2717 54 1484 165 55
Fragments arranged by size:
2717 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 2473 2654 2473
Size: 181 4294
BsrGI T'GTAC_A
Cuts at: 461 461
Size: 4475
BssSI C'ACGA_G
Cuts at: 2746 3454 2746
Size: 708 3767
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstEII G'GTnAC_C
Cuts at: 144 144
Size: 4475
BstYI r'GATC_y
Cuts at: 1 219 252 1500 1967 1990 2325 2571 2877
Size: 218 33 1248 467 23 335 246 306
Cuts at: 2877 2889 2975 4027 1
Size: 12 86 1052 449
Fragments arranged by size:
1248 1052 467 449 335 306 246 218
86 33 23 12
BtgI C'CryG_G
Cuts at: 107 650 827 1307 107
Size: 543 177 480 3275
Fragments arranged by size:
3275 543 480 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 605 2425 2606 2769 605
Size: 1820 181 163 2311
Fragments arranged by size:
2311 1820 181 163
BtsI GCAGTG_nn'
Cuts at: 547 843 1333 1718 547
Size: 296 490 385 3304
Fragments arranged by size:
3304 490 385 296
DraI TTT'AAA
Cuts at: 435 1128 1474 435
Size: 693 346 3436
Fragments arranged by size:
3436 693 346
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3525 2181
Size: 1344 3131
EaeI y'GGCC_r
Cuts at: 842 2060 2234 2625 2652 3997 842
Size: 1218 174 391 27 1345 1320
Fragments arranged by size:
1345 1320 1218 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 1073 1139 2498 2708 1073
Size: 66 1359 210 2840
Fragments arranged by size:
2840 1359 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 3425 3571 4285 2683
Size: 742 146 714 2873
Fragments arranged by size:
2873 742 714 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1163 1277 1360 2300 2732 3085 3747 1027
Size: 136 114 83 940 432 353 662 1755
Fragments arranged by size:
1755 940 662 432 353 136 114 83
EcoO109I rG'GnC_Cy
Cuts at: 506 927 928 1015 1614 506
Size: 421 1 87 599 3367
Fragments arranged by size:
3367 599 421 87 1
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 362 2494 362
Size: 2132 2343
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 1909 2157 3387 4158 1909
Size: 248 1230 771 2226
Fragments arranged by size:
2226 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2317 2347 2349 2379 2317
Size: 30 2 30 4413
Fragments arranged by size:
4413 30 30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
HindIII A'AGCT_T
Cuts at: 852 852
Size: 4475
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 298 1495 298
Size: 1197 3278
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 536 3234 3418 536
Size: 2698 184 1593
Fragments arranged by size:
2698 1593 184
MscI TGG'CCA
Cuts at: 2236 2236
Size: 4475
MslI CAynn'nnrTG
Cuts at: 538 1397 2464 2591 538
Size: 859 1067 127 2422
Fragments arranged by size:
2422 1067 859 127
MspA1I CmG'CkG
Cuts at: 1586 1900 2260 3044 3289 1586
Size: 314 360 784 245 2772
Fragments arranged by size:
2772 784 360 314 245
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NcoI C'CATG_G
Cuts at: 107 650 827 1307 107
Size: 543 177 480 3275
Fragments arranged by size:
3275 543 480 177
NdeI CA'TA_TG
Cuts at: 900 900
Size: 4475
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NsiI A_TGCA'T
Cuts at: 561 561
Size: 4475
NspI r_CATG'y
Cuts at: 1419 1528 2028 3631 1419
Size: 109 500 1603 2263
Fragments arranged by size:
2263 1603 500 109
PciI A'CATG_T
Cuts at: 3627 3627
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 1015 1015
Size: 4475
PspOMI G'GGCC_C
Cuts at: 927 927
Size: 4475
PstI C_TGCA'G
Cuts at: 418 1522 2207 418
Size: 1104 685 2686
Fragments arranged by size:
2686 1104 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 1900 2260 1900
Size: 360 4115
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 1073 2498 2708 1073
Size: 1425 210 2840
Fragments arranged by size:
2840 1425 210
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 414 1518 2203 3171 3362 33
Size: 381 1104 685 968 191 1146
Fragments arranged by size:
1146 1104 968 685 381 191
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 1630 2545 3259 3521 1630
Size: 915 714 262 2584
Fragments arranged by size:
2584 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StyI C'CwwG_G
Cuts at: 107 650 718 827 1307 1609 1963 107
Size: 543 68 109 480 302 354 2619
Fragments arranged by size:
2619 543 480 354 302 109 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2058 2191 2650 2859 2058
Size: 133 459 209 3674
Fragments arranged by size:
3674 459 209 133
TatI w'GTAC_w
Cuts at: 461 3990 461
Size: 3529 946
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 4443
Tth111I GACn'n_nGTC
Cuts at: 2272 2272
Size: 4475
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
XmnI GAAnn'nnTTC
Cuts at: 245 245
Size: 4475
ZraI GAC'GTC
Cuts at: 730 730
Size: 4475
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsgI
BsiWI BsmBI BspEI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI