Restriction map for
pF1KB3956
(Circular) (Six-base) MAPSORT of: pF1KB3956.seq Check: 9627 from: 1 to: 4265
pF1KB3956 4265 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1303 1303
Size: 4265
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 1276 2090 2522 3269 3537 174
Size: 1102 814 432 747 268 902
Fragments arranged by size:
1102 902 814 747 432 268
AflIII A'CryG_T
Cuts at: 1617 2721 3698 1617
Size: 1104 977 2184
Fragments arranged by size:
2184 1104 977
AgeI A'CCGG_T
Cuts at: 2653 2653
Size: 4265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 922 922
Size: 4265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 844 876 844
Size: 32 4233
AlwNI CAG_nnn'CTG
Cuts at: 874 1570 3137 874
Size: 696 1567 2002
Fragments arranged by size:
2002 1567 696
ApaI G_GGCC'C
Cuts at: 811 811
Size: 4265
ApaLI G'TGCA_C
Cuts at: 3035 3035
Size: 4265
ApoI r'AATT_y
Cuts at: 1269 3618 3874 1269
Size: 2349 256 1660
Fragments arranged by size:
2349 1660 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 848 1285 848
Size: 437 3828
BamHI G'GATC_C
Cuts at: 1290 1290
Size: 4265
BanI G'GyrC_C
Cuts at: 972 1221 1281 1695 1943 1978 972
Size: 249 60 414 248 35 3259
Fragments arranged by size:
3259 414 249 248 60 35
BanII G_rGCy'C
Cuts at: 811 857 1279 2309 811
Size: 46 422 1030 2767
Fragments arranged by size:
2767 1030 422 46
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BbsI GAAGACnn'nnnn_
Cuts at: 928 928
Size: 4265
BciVI GTATCCnnnnn_n'
Cuts at: 2163 2924 4265 2163
Size: 761 1341 2163
Fragments arranged by size:
2163 1341 761
BclI T'GATC_A
Cuts at: 1088 1785 1088
Size: 697 3568
BglI GCCn_nnn'nGGC
Cuts at: 391 547 391
Size: 156 4109
BglII A'GATC_T
Cuts at: 722 737 1780 3817 722
Size: 15 1043 2037 1170
Fragments arranged by size:
2037 1170 1043 15
BlpI GC'TnA_GC
Cuts at: 1377 1377
Size: 4265
Bme1580I G_kGCm'C
Cuts at: 811 975 1226 1890 1983 3039 811
Size: 164 251 664 93 1056 2037
Fragments arranged by size:
2037 1056 664 251 164 93
BmrI ACTGGGnnnn_n'
Cuts at: 1477 1558 1893 4037 1477
Size: 81 335 2144 1705
Fragments arranged by size:
2144 1705 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 4265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 2812 3110 1441
Size: 879 492 298 2596
Fragments arranged by size:
2596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3969 3969
Size: 4265
BsaAI yAC'GTr
Cuts at: 450 483 1056 1618 450
Size: 33 573 562 3097
Fragments arranged by size:
3097 573 562 33
BsaBI GATnn'nnATC
Cuts at: 6 1779 3816 6
Size: 1773 2037 455
Fragments arranged by size:
2037 1773 455
BsaHI Gr'CG_yC
Cuts at: 1164 1696 1944 2646 4142 1164
Size: 532 248 702 1496 1287
Fragments arranged by size:
1496 1287 702 532 248
BsaWI w'CCGG_w
Cuts at: 1454 1975 2653 2927 3074 3485 1454
Size: 521 678 274 147 411 2234
Fragments arranged by size:
2234 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 844 874 154
Size: 30 660 30 3545
Fragments arranged by size:
3545 660 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 162
Size: 4265
BseYI C'CCAG_C
Cuts at: 968 1172 1690 2448 3025 3521 968
Size: 204 518 758 577 496 1712
Fragments arranged by size:
1712 758 577 518 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1141
Size: 4265
BsiEI CG_ry'CG
Cuts at: 104 1807 1853 3061 104
Size: 1703 46 1208 1308
Fragments arranged by size:
1703 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 488 857 938 1279 2057 2247 3039 488
Size: 369 81 341 778 190 792 1714
Fragments arranged by size:
1714 792 778 369 341 190 81
BsmI GAATG_Cn'
Cuts at: 944 944
Size: 4265
BsmBI CGTCTCn'nnnn_
Cuts at: 522 534 522
Size: 12 4253
Bsp1286I G_dGCh'C
Cuts at: 488 811 857 938 975 1226 1279 1890 1983
Size: 323 46 81 37 251 53 611 93
Cuts at: 1983 2057 2247 2309 3039 488
Size: 74 190 62 730 1714
Fragments arranged by size:
1714 730 611 323 251 190 93 81
74 62 53 46 37
BspEI T'CCGG_A
Cuts at: 3485 3485
Size: 4265
BspHI T'CATG_A
Cuts at: 3441 3441
Size: 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 515 1315 1529 1831 2212 515
Size: 800 214 302 381 2568
Fragments arranged by size:
2568 800 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2557 2611 4095 4260 50
Size: 2507 54 1484 165 55
Fragments arranged by size:
2507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 278 380 551 656 2177 278
Size: 102 171 105 1521 2366
Fragments arranged by size:
2366 1521 171 105 102
BsrFI r'CCGG_y
Cuts at: 2263 2444 2653 2263
Size: 181 209 3875
Fragments arranged by size:
3875 209 181
BssSI C'ACGA_G
Cuts at: 2536 2894 2536
Size: 358 3907
BstAPI GCAn_nnn'nTGC
Cuts at: 1642 1642
Size: 4265
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstEII G'GTnAC_C
Cuts at: 816 816
Size: 4265
BstYI r'GATC_y
Cuts at: 1 722 737 842 1290 1757 1780 2115 2361
Size: 721 15 105 448 467 23 335 246
Cuts at: 2361 3373 3459 3471 3817 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 721 467 449 448 346 335 246
105 86 23 15 12
BtgI C'CryG_G
Cuts at: 107 595 107
Size: 488 3777
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1053 2215 2396 2559 1053
Size: 1162 181 163 2759
Fragments arranged by size:
2759 1162 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1508 1043
Size: 465 3800
DraI TTT'AAA
Cuts at: 1264 1264
Size: 4265
DraIII CAC_nnn'GTG
Cuts at: 1100 1100
Size: 4265
DrdI GACnn_nn'nnGTC
Cuts at: 1971 2829 1971
Size: 858 3407
EaeI y'GGCC_r
Cuts at: 392 413 477 548 732 1850 2024 2415 2442
Size: 21 64 71 184 1118 174 391 27
Cuts at: 2442 3787 392
Size: 1345 870
Fragments arranged by size:
1345 1118 870 391 184 174 71 64
27 21
EagI C'GGCC_G
Cuts at: 1850 1850
Size: 4265
EarI CTCTTCn'nnn_
Cuts at: 787 1229 2288 2498 787
Size: 442 1059 210 2554
Fragments arranged by size:
2554 1059 442 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2473 2783 2929 4075 2473
Size: 310 146 1146 2663
Fragments arranged by size:
2663 1146 310 146
EcoICRI GAG'CTC
Cuts at: 855 1277 855
Size: 422 3843
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 1276 2090 2522 3269 3537 174
Size: 1102 814 432 747 268 902
Fragments arranged by size:
1102 902 814 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 808 1404 808
Size: 596 3669
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
EcoRV GAT'ATC
Cuts at: 568 568
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 792 2284 792
Size: 1492 2773
FspI TGC'GCA
Cuts at: 361 511 2046 361
Size: 150 1535 2580
Fragments arranged by size:
2580 1535 150
HaeII r_GCGC'y
Cuts at: 1699 1947 2969 3948 1699
Size: 248 1022 979 2016
Fragments arranged by size:
2016 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 591 623 2107 2137 2139 2169 154
Size: 32 405 32 1484 30 2 30 2250
Fragments arranged by size:
2250 1484 405 32 32 30 30 2
HincII GTy'rAC
Cuts at: 501 1304 501
Size: 803 3462
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 425 2936 3120 425
Size: 2511 184 1570
Fragments arranged by size:
2511 1570 184
MscI TGG'CCA
Cuts at: 394 415 479 550 734 2026 394
Size: 21 64 71 184 1292 2633
Fragments arranged by size:
2633 1292 184 71 64 21
MslI CAynn'nnrTG
Cuts at: 1072 2254 2381 1072
Size: 1182 127 2956
Fragments arranged by size:
2956 1182 127
MspA1I CmG'CkG
Cuts at: 1176 1376 1690 2050 3063 3308 1176
Size: 200 314 360 1013 245 2133
Fragments arranged by size:
2133 1013 360 314 245 200
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NcoI C'CATG_G
Cuts at: 107 107
Size: 4265
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NspI r_CATG'y
Cuts at: 1318 1818 2725 1318
Size: 500 907 2858
Fragments arranged by size:
2858 907 500
PasI CC'CwG_GG
Cuts at: 874 874
Size: 4265
PciI A'CATG_T
Cuts at: 2721 2721
Size: 4265
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PmlI CAC'GTG
Cuts at: 483 483
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 844 876 3404 3436 844
Size: 32 2528 32 1673
Fragments arranged by size:
2528 1673 32 32
PspOMI G'GGCC_C
Cuts at: 807 807
Size: 4265
PstI C_TGCA'G
Cuts at: 1312 1997 1312
Size: 685 3580
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 1690 2050 1690
Size: 360 3905
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 857 1279 857
Size: 422 3843
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SapI GCTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 1135 3782 1135
Size: 2647 1618
SfcI C'TryA_G
Cuts at: 33 1308 1993 2986 3177 33
Size: 1275 685 993 191 1121
Fragments arranged by size:
1275 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 850 1287 850
Size: 437 3828
SmlI C'TyrA_G
Cuts at: 1420 2335 2827 3089 1420
Size: 915 492 262 2596
Fragments arranged by size:
2596 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 1318 1818 1318
Size: 500 3765
StyI C'CwwG_G
Cuts at: 107 632 1399 1753 107
Size: 525 767 354 2619
Fragments arranged by size:
2619 767 525 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 156 1848 1981 2440 2649 156
Size: 1692 133 459 209 1772
Fragments arranged by size:
1772 1692 459 209 133
TatI w'GTAC_w
Cuts at: 1133 3780 1133
Size: 2647 1618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4233
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 543 1236 1760 543
Size: 693 524 3048
Fragments arranged by size:
3048 693 524
XmaI C'CCGG_G
Cuts at: 848 1285 848
Size: 437 3828
XmnI GAAnn'nnTTC
Cuts at: 724 1135 724
Size: 411 3854
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I
BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI