Restriction map for
pF1KB3962
(Circular) (Six-base) MAPSORT of: pF1KB3962.seq Check: 4243 from: 1 to: 4628
pF1KB3962 4628 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 535 1666 535
Size: 1131 3497
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1280 1514 2453 2885 3238 3900 389
Size: 891 234 939 432 353 662 1117
Fragments arranged by size:
1117 939 891 662 432 353 234
AflIII A'CryG_T
Cuts at: 123 1980 3780 4061 123
Size: 1857 1800 281 690
Fragments arranged by size:
1857 1800 690 281
AleI CACnn'nnGTG
Cuts at: 868 868
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 1519 1567 1933 3371 1519
Size: 48 366 1438 2776
Fragments arranged by size:
2776 1438 366 48
ApaLI G'TGCA_C
Cuts at: 3466 3466
Size: 4628
ApoI r'AATT_y
Cuts at: 112 129 268 391 1632 3981 4237 112
Size: 17 139 123 1241 2349 256 503
Fragments arranged by size:
2349 1241 503 256 139 123 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 1648 1648
Size: 4628
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 708 870 1644 2058 2306 2341 708
Size: 162 774 414 248 35 2995
Fragments arranged by size:
2995 774 414 248 162 35
BanII G_rGCy'C
Cuts at: 1642 2672 1642
Size: 1030 3598
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 1268 1430 1268
Size: 162 4466
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3582 4628 2526
Size: 1056 1046 2526
Fragments arranged by size:
2526 1056 1046
BclI T'GATC_A
Cuts at: 306 2148 306
Size: 1842 2786
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 2253 2346 3470 2253
Size: 93 1124 3411
Fragments arranged by size:
3411 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1596 1840 1921 2256 4400 1596
Size: 244 81 335 2144 1824
Fragments arranged by size:
2144 1824 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 1076 953
Size: 123 4505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 672 1287 1804 2683 3397 3695 633
Size: 39 615 517 879 714 298 1566
Fragments arranged by size:
1566 879 714 615 517 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 283 1981 283
Size: 1698 2930
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3427 3574 3848 1817
Size: 521 678 411 147 274 2597
Fragments arranged by size:
2597 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1065 1095 1065
Size: 30 4598
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 493
Size: 4628
BseYI C'CCAG_C
Cuts at: 956 2053 2811 3476 3884 956
Size: 1097 758 665 408 1700
Fragments arranged by size:
1700 1097 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 560
Size: 4628
BsiEI CG_ry'CG
Cuts at: 104 1042 2170 2216 3446 104
Size: 938 1128 46 1230 1286
Fragments arranged by size:
1286 1230 1128 938 46
BsiHKAI G_wGCw'C
Cuts at: 1642 2420 2610 3470 1642
Size: 778 190 860 2800
Fragments arranged by size:
2800 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1642 2253 2346 2420 2610 2672 3470 1642
Size: 611 93 74 190 62 798 2800
Fragments arranged by size:
2800 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1678 1892 2194 2575 1678
Size: 214 302 381 3731
Fragments arranged by size:
3731 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2540 2540
Size: 4628
BsrFI r'CCGG_y
Cuts at: 2626 2807 2626
Size: 181 4447
BssSI C'ACGA_G
Cuts at: 2899 3607 2899
Size: 708 3920
BstAPI GCAn_nnn'nTGC
Cuts at: 574 2005 574
Size: 1431 3197
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstYI r'GATC_y
Cuts at: 1 1155 1653 2120 2143 2478 2724 3030 3042
Size: 1154 498 467 23 335 246 306 12
Cuts at: 3042 3128 4180 1
Size: 86 1052 449
Fragments arranged by size:
1154 1052 498 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1614 1614
Size: 4628
BtgI C'CryG_G
Cuts at: 866 866
Size: 4628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 1871 1871
Size: 4628
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3678 2334
Size: 1344 3284
EaeI y'GGCC_r
Cuts at: 204 2213 2387 2778 2805 4150 204
Size: 2009 174 391 27 1345 682
Fragments arranged by size:
2009 1345 682 391 174 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 761 1134 2651 2861 761
Size: 373 1517 210 2528
Fragments arranged by size:
2528 1517 373 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1564 2836 3578 3724 4438 1564
Size: 1272 742 146 714 1754
Fragments arranged by size:
1754 1272 742 714 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 953 1076 1280 1514 2453 2885 3238 3900
Size: 564 123 204 234 939 432 353 662
Cuts at: 3900 389
Size: 1117
Fragments arranged by size:
1117 939 662 564 432 353 234 204
123
EcoNI CCTnn'n_nnAGG
Cuts at: 720 740 720
Size: 20 4608
EcoO109I rG'GnC_Cy
Cuts at: 150 895 1767 150
Size: 745 872 3011
Fragments arranged by size:
3011 872 745
EcoRI G'AATT_C
Cuts at: 129 391 1632 129
Size: 262 1241 3125
Fragments arranged by size:
3125 1241 262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 2062 2310 3540 4311 2062
Size: 248 1230 771 2379
Fragments arranged by size:
2379 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2470 2500 2502 2532 2470
Size: 30 2 30 4566
Fragments arranged by size:
4566 30 30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
HindIII A'AGCT_T
Cuts at: 401 401
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MfeI C'AATT_G
Cuts at: 728 728
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1114 3387 3571 1114
Size: 2273 184 2171
Fragments arranged by size:
2273 2171 184
MscI TGG'CCA
Cuts at: 206 2389 206
Size: 2183 2445
MslI CAynn'nnrTG
Cuts at: 251 868 1432 1543 2617 2744 251
Size: 617 564 111 1074 127 2135
Fragments arranged by size:
2135 1074 617 564 127 111
MspA1I CmG'CkG
Cuts at: 838 1287 1329 1739 2053 2413 3197 3442 838
Size: 449 42 410 314 360 784 245 2024
Fragments arranged by size:
2024 784 449 410 360 314 245 42
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 866 866
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NspI r_CATG'y
Cuts at: 127 832 1681 2181 3784 127
Size: 705 849 500 1603 971
Fragments arranged by size:
1603 971 849 705 500
PciI A'CATG_T
Cuts at: 123 3780 123
Size: 3657 971
PfoI T'CCnGG_A
Cuts at: 882 882
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 4596
PpuMI rG'GwC_Cy
Cuts at: 150 895 150
Size: 745 3883
PshAI GACnn'nnGTC
Cuts at: 1044 1044
Size: 4628
PstI C_TGCA'G
Cuts at: 843 1292 1675 2360 843
Size: 449 383 685 3111
Fragments arranged by size:
3111 685 449 383
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 838 1287 1329 2053 2413 838
Size: 449 42 724 360 3053
Fragments arranged by size:
3053 724 449 360 42
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 1134 2651 2861 1134
Size: 1517 210 2901
Fragments arranged by size:
2901 1517 210
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 693 4145 693
Size: 3452 1176
SfcI C'TryA_G
Cuts at: 33 839 1073 1288 1520 1671 2356 3324 3515
Size: 806 234 215 232 151 685 968 191
Cuts at: 3515 33
Size: 1146
Fragments arranged by size:
1146 968 806 685 234 232 215 191
151
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 648 687 1266 1783 2698 3412 3674 648
Size: 39 579 517 915 714 262 1602
Fragments arranged by size:
1602 915 714 579 517 262 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
StyI C'CwwG_G
Cuts at: 866 1508 1762 2116 866
Size: 642 254 354 3378
Fragments arranged by size:
3378 642 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 542 718 1464 1555 2211 2344 2803 3012 542
Size: 176 746 91 656 133 459 209 2158
Fragments arranged by size:
2158 746 656 459 209 176 133 91
TatI w'GTAC_w
Cuts at: 187 691 915 1120 1558 4143 187
Size: 504 224 205 438 2585 672
Fragments arranged by size:
2585 672 504 438 224 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 150 182 4145 4177 150
Size: 32 3963 32 601
Fragments arranged by size:
3963 601 32 32
Tth111I GACn'n_nGTC
Cuts at: 292 1023 2425 292
Size: 731 1402 2495
Fragments arranged by size:
2495 1402 731
XbaI T'CTAG_A
Cuts at: 432 1659 432
Size: 1227 3401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1123 2123 1123
Size: 1000 3628
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 391 391
Size: 4628
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI