Restriction map for
pF1KB3963
(Circular) (Six-base) MAPSORT of: pF1KB3963.seq Check: 7137 from: 1 to: 5252
pF1KB3963 5252 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1056 1056
Size: 5252
AatII G_ACGT'C
Cuts at: 1469 1469
Size: 5252
AccI GT'mk_AC
Cuts at: 1745 2290 1745
Size: 545 4707
Acc65I G'GTAC_C
Cuts at: 2268 2268
Size: 5252
AclI AA'CG_TT
Cuts at: 5092 5092
Size: 5252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 1898 2181 2214 3077 3509 3862 4524 255
Size: 1643 283 33 863 432 353 662 983
Fragments arranged by size:
1643 983 863 662 432 353 283 33
AflIII A'CryG_T
Cuts at: 998 2604 4404 4685 998
Size: 1606 1800 281 1565
Fragments arranged by size:
1800 1606 1565 281
AhdI GACnn_n'nnGTC
Cuts at: 1731 1731
Size: 5252
AleI CACnn'nnGTG
Cuts at: 895 1039 1717 895
Size: 144 678 4430
Fragments arranged by size:
4430 678 144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1609 1609
Size: 5252
AlwNI CAG_nnn'CTG
Cuts at: 156 278 1876 2557 3995 156
Size: 122 1598 681 1438 1413
Fragments arranged by size:
1598 1438 1413 681 122
ApaI G_GGCC'C
Cuts at: 1531 2190 1531
Size: 659 4593
ApaLI G'TGCA_C
Cuts at: 4090 4090
Size: 5252
ApoI r'AATT_y
Cuts at: 235 484 665 1192 1406 1963 2157 2256 4605
Size: 249 181 527 214 557 194 99 2349
Cuts at: 4605 4861 235
Size: 256 626
Fragments arranged by size:
2349 626 557 527 256 249 214 194
181 99
AseI AT'TA_AT
Cuts at: 20 20
Size: 5252
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5252
AvaI C'yCGr_G
Cuts at: 312 781 1327 2272 312
Size: 469 546 945 3292
Fragments arranged by size:
3292 945 546 469
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2128 2161 2128
Size: 33 5219
BamHI G'GATC_C
Cuts at: 2277 2277
Size: 5252
BanI G'GyrC_C
Cuts at: 792 889 2268 2682 2930 2965 792
Size: 97 1379 414 248 35 3079
Fragments arranged by size:
3079 1379 414 248 97 35
BanII G_rGCy'C
Cuts at: 319 466 1531 2190 2266 3296 319
Size: 147 1065 659 76 1030 2275
Fragments arranged by size:
2275 1065 1030 659 147 76
BbeI G_GCGC'C
Cuts at: 2686 2934 2686
Size: 248 5004
BbsI GAAGACnn'nnnn_
Cuts at: 1454 1527 1454
Size: 73 5179
BbvCI CC'TCA_GC
Cuts at: 2221 2221
Size: 5252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 576 610 762 796 90
Size: 34 452 34 152 34 4546
Fragments arranged by size:
4546 452 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3150 4206 5252 3150
Size: 1056 1046 3150
Fragments arranged by size:
3150 1056 1046
BclI T'GATC_A
Cuts at: 816 1094 1179 1430 2772 816
Size: 278 85 251 1342 3296
Fragments arranged by size:
3296 1342 278 251 85
BglII A'GATC_T
Cuts at: 2006 2127 2767 4804 2006
Size: 121 640 2037 2454
Fragments arranged by size:
2454 2037 640 121
BlpI GC'TnA_GC
Cuts at: 2235 2364 2235
Size: 129 5123
Bme1580I G_kGCm'C
Cuts at: 1531 1810 2190 2877 2970 4094 1531
Size: 279 380 687 93 1124 2689
Fragments arranged by size:
2689 1124 687 380 279 93
BmgBI CAC'GTC
Cuts at: 867 1839 867
Size: 972 4280
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1820 2464 2545 2880 5024 1525
Size: 295 644 81 335 2144 1753
Fragments arranged by size:
2144 1753 644 335 295 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1593 3619 1593
Size: 2026 3226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1852 1852
Size: 5252
Bpu10I CC'TnA_GC
Cuts at: 2221 2221
Size: 5252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 2428 3307 4021 4319 1091
Size: 1337 879 714 298 2024
Fragments arranged by size:
2024 1337 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 315 748 4956 315
Size: 433 4208 611
Fragments arranged by size:
4208 611 433
BsaAI yAC'GTr
Cuts at: 2605 2605
Size: 5252
BsaBI GATnn'nnATC
Cuts at: 6 1162 1934 2766 4803 6
Size: 1156 772 832 2037 455
Fragments arranged by size:
2037 1156 832 772 455
BsaHI Gr'CG_yC
Cuts at: 976 1466 2683 2931 3633 5129 976
Size: 490 1217 248 702 1496 1099
Fragments arranged by size:
1496 1217 1099 702 490 248
BsaWI w'CCGG_w
Cuts at: 2441 2962 3640 4051 4198 4472 2441
Size: 521 678 411 147 274 3221
Fragments arranged by size:
3221 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1016 1046 1232 1262 1606 1636 1016
Size: 30 186 30 344 30 4632
Fragments arranged by size:
4632 344 186 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2209 2209
Size: 5252
BseYI C'CCAG_C
Cuts at: 930 1480 1665 2677 3435 4100 4508 930
Size: 550 185 1012 758 665 408 1674
Fragments arranged by size:
1674 1012 758 665 550 408 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 171
Size: 5252
BsiEI CG_ry'CG
Cuts at: 104 835 2794 2840 4070 104
Size: 731 1959 46 1230 1286
Fragments arranged by size:
1959 1286 1230 731 46
BsiHKAI G_wGCw'C
Cuts at: 2149 2266 3044 3234 4094 2149
Size: 117 778 190 860 3307
Fragments arranged by size:
3307 860 778 190 117
BsmI GAATG_Cn'
Cuts at: 1611 1611
Size: 5252
BsmBI CGTCTCn'nnnn_
Cuts at: 702 1473 702
Size: 771 4481
Bsp1286I G_dGCh'C
Cuts at: 319 466 1531 1810 2149 2190 2266 2877 2970
Size: 147 1065 279 339 41 76 611 93
Cuts at: 2970 3044 3234 3296 4094 319
Size: 74 190 62 798 1477
Fragments arranged by size:
1477 1065 798 611 339 279 190 147
93 76 74 62 41
BspHI T'CATG_A
Cuts at: 1160 3684 1160
Size: 2524 2728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1056 1660 2084 2302 2516 2818 3199 1056
Size: 604 424 218 214 302 381 3109
Fragments arranged by size:
3109 604 424 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 126 3544 3598 5082 5247 50
Size: 76 3418 54 1484 165 55
Fragments arranged by size:
3418 1484 165 76 55 54
BsrDI GCAATG_nn'
Cuts at: 3164 3164
Size: 5252
BsrFI r'CCGG_y
Cuts at: 789 3250 3431 789
Size: 2461 181 2610
Fragments arranged by size:
2610 2461 181
BsrGI T'GTAC_A
Cuts at: 1757 1757
Size: 5252
BssSI C'ACGA_G
Cuts at: 433 3523 4231 433
Size: 3090 708 1454
Fragments arranged by size:
3090 1454 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2629 2629
Size: 5252
BstBI TT'CG_AA
Cuts at: 3613 3613
Size: 5252
BstEII G'GTnAC_C
Cuts at: 878 1557 878
Size: 679 4573
BstXI CCAn_nnnn'nTGG
Cuts at: 1673 1673
Size: 5252
BstYI r'GATC_y
Cuts at: 1 1935 1953 2006 2127 2277 2744 2767 3102
Size: 1934 18 53 121 150 467 23 335
Cuts at: 3102 3348 3654 3666 3752 4804 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1934 1052 467 449 335 306 246 150
121 86 53 23 18 12
BtgI C'CryG_G
Cuts at: 1610 1610
Size: 5252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 353 3202 3383 3546 353
Size: 2849 181 163 2059
Fragments arranged by size:
2849 2059 181 163
BtsI GCAGTG_nn'
Cuts at: 380 1760 2495 380
Size: 1380 735 3137
Fragments arranged by size:
3137 1380 735
ClaI AT'CG_AT
Cuts at: 1010 1166 1010
Size: 156 5096
DraI TTT'AAA
Cuts at: 483 2251 483
Size: 1768 3484
DrdI GACnn_nn'nnGTC
Cuts at: 2958 4302 2958
Size: 1344 3908
EaeI y'GGCC_r
Cuts at: 861 1145 2837 3011 3402 3429 4774 861
Size: 284 1692 174 391 27 1345 1339
Fragments arranged by size:
1692 1345 1339 391 284 174 27
EagI C'GGCC_G
Cuts at: 2837 2837
Size: 5252
EarI CTCTTCn'nnn_
Cuts at: 1774 2233 3275 3485 1774
Size: 459 1042 210 3541
Fragments arranged by size:
3541 1042 459 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3460 4202 4348 5062 3460
Size: 742 146 714 3650
Fragments arranged by size:
3650 742 714 146
EcoICRI GAG'CTC
Cuts at: 2264 2264
Size: 5252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 1852 1898 2181 2214 3077 3509 3862 4524
Size: 1597 46 283 33 863 432 353 662
Cuts at: 4524 255
Size: 983
Fragments arranged by size:
1597 983 863 662 432 353 283 46
33
EcoNI CCTnn'n_nnAGG
Cuts at: 148 1019 148
Size: 871 4381
EcoO109I rG'GnC_Cy
Cuts at: 850 1527 1572 1662 2186 2187 2391 850
Size: 677 45 90 524 1 204 3711
Fragments arranged by size:
3711 677 524 204 90 45 1
EcoRI G'AATT_C
Cuts at: 235 2157 2256 235
Size: 1922 99 3231
Fragments arranged by size:
3231 1922 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1393 3271 1393
Size: 1878 3374
FspI TGC'GCA
Cuts at: 3033 3033
Size: 5252
HaeII r_GCGC'y
Cuts at: 2686 2934 4164 4935 2686
Size: 248 1230 771 3003
Fragments arranged by size:
3003 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 713 745 973 1005 1016 1048 1232
Size: 32 446 32 228 32 11 32 184
Cuts at: 1232 1264 1606 1638 1717 1749 3094 3124 3126
Size: 32 342 32 79 32 1345 30 2
Cuts at: 3126 3156 235
Size: 30 2331
Fragments arranged by size:
2331 1345 446 342 228 184 79 32
32 32 32 32 32 32 30 30
11 2
HincII GTy'rAC
Cuts at: 1521 1707 1746 2291 1521
Size: 186 39 545 4482
Fragments arranged by size:
4482 545 186 39
KasI G'GCGC_C
Cuts at: 2682 2930 2682
Size: 248 5004
KpnI G_GTAC'C
Cuts at: 2272 2272
Size: 5252
MfeI C'AATT_G
Cuts at: 1778 1778
Size: 5252
MluI A'CGCG_T
Cuts at: 4685 4685
Size: 5252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1110 4011 4195 1110
Size: 2901 184 2167
Fragments arranged by size:
2901 2167 184
MscI TGG'CCA
Cuts at: 3013 3013
Size: 5252
MslI CAynn'nnrTG
Cuts at: 895 898 1039 1159 1555 1717 2086 3241 3368
Size: 3 141 120 396 162 369 1155 127
Cuts at: 3368 895
Size: 2779
Fragments arranged by size:
2779 1155 396 369 162 141 127 120
3
MspA1I CmG'CkG
Cuts at: 232 1717 2363 2677 3037 3821 4066 232
Size: 1485 646 314 360 784 245 1418
Fragments arranged by size:
1485 1418 784 646 360 314 245
NaeI GCC'GGC
Cuts at: 3433 3433
Size: 5252
NarI GG'CG_CC
Cuts at: 2683 2931 2683
Size: 248 5004
NgoMIV G'CCGG_C
Cuts at: 3431 3431
Size: 5252
NspI r_CATG'y
Cuts at: 1002 2305 2805 4408 1002
Size: 1303 500 1603 1846
Fragments arranged by size:
1846 1603 1303 500
PciI A'CATG_T
Cuts at: 998 4404 998
Size: 3406 1846
PflMI CCAn_nnn'nTGG
Cuts at: 870 870
Size: 5252
PfoI T'CCnGG_A
Cuts at: 192 1511 192
Size: 1319 3933
PmeI GTTT'AAAC
Cuts at: 2251 2251
Size: 5252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 5220
PpuMI rG'GwC_Cy
Cuts at: 1572 1662 1572
Size: 90 5162
PspOMI G'GGCC_C
Cuts at: 1527 2186 1527
Size: 659 4593
PstI C_TGCA'G
Cuts at: 174 375 1631 2155 2299 2984 174
Size: 201 1256 524 144 685 2442
Fragments arranged by size:
2442 1256 685 524 201 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5252
PvuII CAG'CTG
Cuts at: 232 1717 2677 3037 232
Size: 1485 960 360 2447
Fragments arranged by size:
2447 1485 960 360
RsrII CG'GwC_CG
Cuts at: 3447 3447
Size: 5252
SacI G_AGCT'C
Cuts at: 2266 2266
Size: 5252
SalI G'TCGA_C
Cuts at: 1744 2289 1744
Size: 545 4707
SapI GCTCTTCn'nnn_
Cuts at: 3275 3485 3275
Size: 210 5042
SbfI CC_TGCA'GG
Cuts at: 2155 2299 2155
Size: 144 5108
ScaI AGT'ACT
Cuts at: 4769 4769
Size: 5252
SfcI C'TryA_G
Cuts at: 33 170 371 1627 2151 2295 2980 3948 4139
Size: 137 201 1256 524 144 685 968 191
Cuts at: 4139 33
Size: 1146
Fragments arranged by size:
1256 1146 968 685 524 201 191 144
137
SfoI GGC'GCC
Cuts at: 2684 2932 2684
Size: 248 5004
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5252
SmaI CCC'GGG
Cuts at: 1329 2274 1329
Size: 945 4307
SmlI C'TyrA_G
Cuts at: 312 781 1106 2407 3322 4036 4298 312
Size: 469 325 1301 915 714 262 1266
Fragments arranged by size:
1301 1266 915 714 469 325 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5252
SphI G_CATG'C
Cuts at: 2305 2805 2305
Size: 500 4752
SspI AAT'ATT
Cuts at: 1683 1683
Size: 5252
StyI C'CwwG_G
Cuts at: 713 2386 2740 713
Size: 1673 354 3225
Fragments arranged by size:
3225 1673 354
SwaI ATTT'AAAT
Cuts at: 483 483
Size: 5252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 849 897 1076 2124 2835 2968 3427 3636 849
Size: 48 179 1048 711 133 459 209 2465
Fragments arranged by size:
2465 1048 711 459 209 179 133 48
TatI w'GTAC_w
Cuts at: 1757 4767 1757
Size: 3010 2242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 629 661 4769 4801 629
Size: 32 4108 32 1080
Fragments arranged by size:
4108 1080 32 32
Tth111I GACn'n_nGTC
Cuts at: 1030 3049 1030
Size: 2019 3233
XbaI T'CTAG_A
Cuts at: 2283 2283
Size: 5252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1719 2747 1719
Size: 1028 4224
XhoI C'TCGA_G
Cuts at: 312 781 312
Size: 469 4783
XmaI C'CCGG_G
Cuts at: 1327 2272 1327
Size: 945 4307
XmnI GAAnn'nnTTC
Cuts at: 211 211
Size: 5252
ZraI GAC'GTC
Cuts at: 1467 1467
Size: 5252
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AloI AscI AvrII BfrBI BglI
BmtI BsiWI BspEI BssHII BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI