Restriction map for
pF1KB3965
(Circular) (Six-base) MAPSORT of: pF1KB3965.seq Check: 9385 from: 1 to: 5405
pF1KB3965 5405 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 235 235
Size: 5405
AatII G_ACGT'C
Cuts at: 604 604
Size: 5405
AccI GT'mk_AC
Cuts at: 779 1526 1636 2443 779
Size: 747 110 807 3741
Fragments arranged by size:
3741 807 747 110
Acc65I G'GTAC_C
Cuts at: 2421 2421
Size: 5405
AclI AA'CG_TT
Cuts at: 5245 5245
Size: 5405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 298 662 673 874 3230 3662 4015 4677
Size: 97 364 11 201 2356 432 353 662
Cuts at: 4677 201
Size: 929
Fragments arranged by size:
2356 929 662 432 364 353 201 97
11
AfeI AGC'GCT
Cuts at: 1663 1663
Size: 5405
AflII C'TTAA_G
Cuts at: 886 886
Size: 5405
AflIII A'CryG_T
Cuts at: 803 2757 4557 4838 803
Size: 1954 1800 281 1370
Fragments arranged by size:
1954 1800 1370 281
AleI CACnn'nnGTG
Cuts at: 956 1721 956
Size: 765 4640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1506 1739 1506
Size: 233 5172
AlwNI CAG_nnn'CTG
Cuts at: 276 381 1582 1811 2710 4148 276
Size: 105 1201 229 899 1438 1533
Fragments arranged by size:
1533 1438 1201 899 229 105
ApaLI G'TGCA_C
Cuts at: 4243 4243
Size: 5405
ApoI r'AATT_y
Cuts at: 428 2409 4758 5014 428
Size: 1981 2349 256 819
Fragments arranged by size:
2349 1981 819 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5405
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5405
AvaI C'yCGr_G
Cuts at: 2425 2425
Size: 5405
BamHI G'GATC_C
Cuts at: 2430 2430
Size: 5405
BanI G'GyrC_C
Cuts at: 2421 2835 3083 3118 2421
Size: 414 248 35 4708
Fragments arranged by size:
4708 414 248 35
BanII G_rGCy'C
Cuts at: 482 1217 2419 3449 482
Size: 735 1202 1030 2438
Fragments arranged by size:
2438 1202 1030 735
BbeI G_GCGC'C
Cuts at: 2839 3087 2839
Size: 248 5157
BbsI GAAGACnn'nnnn_
Cuts at: 1161 1295 1340 1161
Size: 134 45 5226
Fragments arranged by size:
5226 134 45
BciVI GTATCCnnnnn_n'
Cuts at: 1134 3303 4359 5405 1134
Size: 2169 1056 1046 1134
Fragments arranged by size:
2169 1134 1056 1046
BclI T'GATC_A
Cuts at: 2925 2925
Size: 5405
BfrBI ATG'CAT
Cuts at: 319 766 2015 319
Size: 447 1249 3709
Fragments arranged by size:
3709 1249 447
BglII A'GATC_T
Cuts at: 1916 2920 4957 1916
Size: 1004 2037 2364
Fragments arranged by size:
2364 2037 1004
BlpI GC'TnA_GC
Cuts at: 2517 2517
Size: 5405
Bme1580I G_kGCm'C
Cuts at: 3030 3123 4247 3030
Size: 93 1124 4188
Fragments arranged by size:
4188 1124 93
BmgBI CAC'GTC
Cuts at: 804 804
Size: 5405
BmrI ACTGGGnnnn_n'
Cuts at: 257 2617 2698 3033 5177 257
Size: 2360 81 335 2144 485
Fragments arranged by size:
2360 2144 485 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2258 3772 2258
Size: 1514 3891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1678 865
Size: 813 4592
Bpu10I CC'TnA_GC
Cuts at: 2216 2216
Size: 5405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 927 1015 2581 3460 4174 4472 927
Size: 88 1566 879 714 298 1860
Fragments arranged by size:
1860 1566 879 714 298 88
BsaI GGTCTCn'nnnn_
Cuts at: 1825 2305 5109 1825
Size: 480 2804 2121
Fragments arranged by size:
2804 2121 480
BsaAI yAC'GTr
Cuts at: 354 2758 354
Size: 2404 3001
BsaBI GATnn'nnATC
Cuts at: 6 1921 2919 4956 6
Size: 1915 998 2037 455
Fragments arranged by size:
2037 1915 998 455
BsaHI Gr'CG_yC
Cuts at: 601 2836 3084 3786 5282 601
Size: 2235 248 702 1496 724
Fragments arranged by size:
2235 1496 724 702 248
BsaWI w'CCGG_w
Cuts at: 1697 2594 3115 3793 4204 4351 4625 1697
Size: 897 521 678 411 147 274 2477
Fragments arranged by size:
2477 897 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1651 1681 1651
Size: 30 5375
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 788 1414 1501 223
Size: 565 626 87 4127
Fragments arranged by size:
4127 626 565 87
BseYI C'CCAG_C
Cuts at: 250 1742 2830 3588 4253 4661 250
Size: 1492 1088 758 665 408 994
Fragments arranged by size:
1492 1088 994 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2947 2993 4223 104
Size: 2843 46 1230 1286
Fragments arranged by size:
2843 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 482 851 1217 2419 3197 3387 4247 482
Size: 369 366 1202 778 190 860 1640
Fragments arranged by size:
1640 1202 860 778 369 366 190
BsmI GAATG_Cn'
Cuts at: 621 1059 621
Size: 438 4967
BsmBI CGTCTCn'nnnn_
Cuts at: 188 188
Size: 5405
Bsp1286I G_dGCh'C
Cuts at: 482 851 1217 2419 3030 3123 3197 3387 3449
Size: 369 366 1202 611 93 74 190 62
Cuts at: 3449 4247 482
Size: 798 1640
Fragments arranged by size:
1640 1202 798 611 369 366 190 93
74 62
BspHI T'CATG_A
Cuts at: 3837 3837
Size: 5405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 158 235 1514 2455 2669 2971 3352 158
Size: 77 1279 941 214 302 381 2211
Fragments arranged by size:
2211 1279 941 381 302 214 77
BsrBI CCG'CTC
Cuts at: 50 3697 3751 5235 5400 50
Size: 3647 54 1484 165 55
Fragments arranged by size:
3647 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 642 1407 3317 642
Size: 765 1910 2730
Fragments arranged by size:
2730 1910 765
BsrFI r'CCGG_y
Cuts at: 114 3403 3584 114
Size: 3289 181 1935
Fragments arranged by size:
3289 1935 181
BsrGI T'GTAC_A
Cuts at: 659 659
Size: 5405
BssSI C'ACGA_G
Cuts at: 3676 4384 3676
Size: 708 4697
BstAPI GCAn_nnn'nTGC
Cuts at: 135 1032 2782 135
Size: 897 1750 2758
Fragments arranged by size:
2758 1750 897
BstBI TT'CG_AA
Cuts at: 3766 3766
Size: 5405
BstEII G'GTnAC_C
Cuts at: 2277 2277
Size: 5405
BstXI CCAn_nnnn'nTGG
Cuts at: 162 843 1692 1983 162
Size: 681 849 291 3584
Fragments arranged by size:
3584 849 681 291
BstYI r'GATC_y
Cuts at: 1 1703 1916 2430 2897 2920 3255 3501 3807
Size: 1702 213 514 467 23 335 246 306
Cuts at: 3807 3819 3905 4957 1
Size: 12 86 1052 449
Fragments arranged by size:
1702 1052 514 467 449 335 306 246
213 86 23 12
BstZ17I GTA'TAC
Cuts at: 780 1637 780
Size: 857 4548
Bsu36I CC'TnA_GG
Cuts at: 1711 1711
Size: 5405
BtgI C'CryG_G
Cuts at: 1820 1820
Size: 5405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1500 3355 3536 3699 1500
Size: 1855 181 163 3206
Fragments arranged by size:
3206 1855 181 163
BtsI GCAGTG_nn'
Cuts at: 821 951 2648 821
Size: 130 1697 3578
Fragments arranged by size:
3578 1697 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 887 922 887
Size: 35 5370
DraI TTT'AAA
Cuts at: 2404 2404
Size: 5405
DrdI GACnn_nn'nnGTC
Cuts at: 220 3111 4455 220
Size: 2891 1344 1170
Fragments arranged by size:
2891 1344 1170
EaeI y'GGCC_r
Cuts at: 1206 2990 3164 3555 3582 4927 1206
Size: 1784 174 391 27 1345 1684
Fragments arranged by size:
1784 1684 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2990 2990
Size: 5405
EarI CTCTTCn'nnn_
Cuts at: 1082 1226 2107 2372 3428 3638 1082
Size: 144 881 265 1056 210 2849
Fragments arranged by size:
2849 1056 881 265 210 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 182 1696 3613 4355 4501 5215 133
Size: 49 1514 1917 742 146 714 323
Fragments arranged by size:
1917 1514 742 714 323 146 49
EcoICRI GAG'CTC
Cuts at: 480 1215 2417 480
Size: 735 1202 3468
Fragments arranged by size:
3468 1202 735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 298 662 673 865 874 1678 3230 3662
Size: 97 364 11 192 9 804 1552 432
Cuts at: 3662 4015 4677 201
Size: 353 662 929
Fragments arranged by size:
1552 929 804 662 432 364 353 192
97 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1075 2163 1075
Size: 1088 4317
EcoO109I rG'GnC_Cy
Cuts at: 159 1579 2544 159
Size: 1420 965 3020
Fragments arranged by size:
3020 1420 965
EcoRI G'AATT_C
Cuts at: 2409 2409
Size: 5405
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3424 3424
Size: 5405
FspI TGC'GCA
Cuts at: 3186 3186
Size: 5405
HaeII r_GCGC'y
Cuts at: 1665 2839 3087 4317 5088 1665
Size: 1174 248 1230 771 1982
Fragments arranged by size:
1982 1230 1174 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2283 2315 3247 3277 3279 3309 2283
Size: 32 932 30 2 30 4379
Fragments arranged by size:
4379 932 32 30 30 2
HincII GTy'rAC
Cuts at: 2444 2444
Size: 5405
HindIII A'AGCT_T
Cuts at: 280 792 1244 280
Size: 512 452 4441
Fragments arranged by size:
4441 512 452
KasI G'GCGC_C
Cuts at: 2835 3083 2835
Size: 248 5157
KpnI G_GTAC'C
Cuts at: 2425 2425
Size: 5405
MluI A'CGCG_T
Cuts at: 4838 4838
Size: 5405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4164 4348 4164
Size: 184 5221
MscI TGG'CCA
Cuts at: 1208 3166 1208
Size: 1958 3447
MslI CAynn'nnrTG
Cuts at: 956 1498 1721 2014 3394 3521 956
Size: 542 223 293 1380 127 2840
Fragments arranged by size:
2840 1380 542 293 223 127
MspA1I CmG'CkG
Cuts at: 132 381 2516 2830 3190 3974 4219 132
Size: 249 2135 314 360 784 245 1318
Fragments arranged by size:
2135 1318 784 360 314 249 245
NaeI GCC'GGC
Cuts at: 116 3586 116
Size: 3470 1935
NarI GG'CG_CC
Cuts at: 2836 3084 2836
Size: 248 5157
NcoI C'CATG_G
Cuts at: 1820 1820
Size: 5405
NdeI CA'TA_TG
Cuts at: 1273 2011 1273
Size: 738 4667
NgoMIV G'CCGG_C
Cuts at: 114 3584 114
Size: 3470 1935
NsiI A_TGCA'T
Cuts at: 321 768 2017 321
Size: 447 1249 3709
Fragments arranged by size:
3709 1249 447
NspI r_CATG'y
Cuts at: 1724 2458 2958 4561 1724
Size: 734 500 1603 2568
Fragments arranged by size:
2568 1603 734 500
PasI CC'CwG_GG
Cuts at: 155 1582 1951 155
Size: 1427 369 3609
Fragments arranged by size:
3609 1427 369
PciI A'CATG_T
Cuts at: 4557 4557
Size: 5405
PflMI CCAn_nnn'nTGG
Cuts at: 1568 2310 1568
Size: 742 4663
PfoI T'CCnGG_A
Cuts at: 1136 1136
Size: 5405
PmeI GTTT'AAAC
Cuts at: 2404 2404
Size: 5405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 5373
PpuMI rG'GwC_Cy
Cuts at: 159 159
Size: 5405
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 436 468 436
Size: 32 5373
PstI C_TGCA'G
Cuts at: 131 377 2452 3137 131
Size: 246 2075 685 2399
Fragments arranged by size:
2399 2075 685 246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5405
PvuII CAG'CTG
Cuts at: 132 381 2830 3190 132
Size: 249 2449 360 2347
Fragments arranged by size:
2449 2347 360 249
RsrII CG'GwC_CG
Cuts at: 3600 3600
Size: 5405
SacI G_AGCT'C
Cuts at: 482 1217 2419 482
Size: 735 1202 3468
Fragments arranged by size:
3468 1202 735
SalI G'TCGA_C
Cuts at: 2442 2442
Size: 5405
SapI GCTCTTCn'nnn_
Cuts at: 3428 3638 3428
Size: 210 5195
SbfI CC_TGCA'GG
Cuts at: 2452 2452
Size: 5405
ScaI AGT'ACT
Cuts at: 1549 4922 1549
Size: 3373 2032
SfcI C'TryA_G
Cuts at: 33 127 359 373 1523 2448 3133 4101 4292
Size: 94 232 14 1150 925 685 968 191
Cuts at: 4292 33
Size: 1146
Fragments arranged by size:
1150 1146 968 925 685 232 191 94
14
SfoI GGC'GCC
Cuts at: 2837 3085 2837
Size: 248 5157
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5405
SmaI CCC'GGG
Cuts at: 2427 2427
Size: 5405
SmlI C'TyrA_G
Cuts at: 886 906 994 2560 3475 4189 4451 886
Size: 20 88 1566 915 714 262 1840
Fragments arranged by size:
1840 1566 915 714 262 88 20
SnaBI TAC'GTA
Cuts at: 354 354
Size: 5405
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5405
SphI G_CATG'C
Cuts at: 2458 2958 2458
Size: 500 4905
SspI AAT'ATT
Cuts at: 339 339
Size: 5405
StuI AGG'CCT
Cuts at: 842 2158 842
Size: 1316 4089
StyI C'CwwG_G
Cuts at: 498 684 1820 2153 2539 2893 498
Size: 186 1136 333 386 354 3010
Fragments arranged by size:
3010 1136 386 354 333 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1087 1574 2988 3121 3580 3789 1087
Size: 487 1414 133 459 209 2703
Fragments arranged by size:
2703 1414 487 459 209 133
TatI w'GTAC_w
Cuts at: 659 1547 1943 4920 659
Size: 888 396 2977 1144
Fragments arranged by size:
2977 1144 888 396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 813 845 4922 4954 813
Size: 32 4077 32 1264
Fragments arranged by size:
4077 1264 32 32
Tth111I GACn'n_nGTC
Cuts at: 3202 3202
Size: 5405
XbaI T'CTAG_A
Cuts at: 2436 2436
Size: 5405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2091 2900 2091
Size: 809 4596
XmaI C'CCGG_G
Cuts at: 2425 2425
Size: 5405
ZraI GAC'GTC
Cuts at: 602 602
Size: 5405
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AgeI AhdI AloI ApaI AscI AvrII BaeI BbvCI
BcgI BglI BmtI BsgI BsiWI BspEI BssHII ClaI
DraIII EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI PacI PmlI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SrfI SwaI XhoI XmnI