Restriction map for
pF1KB3975
(Circular) (Six-base) MAPSORT of: pF1KB3975.seq Check: 6269 from: 1 to: 5000
pF1KB3975 5000 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1486 1486
Size: 5000
AccI GT'mk_AC
Cuts at: 293 1064 2038 293
Size: 771 974 3255
Fragments arranged by size:
3255 974 771
Acc65I G'GTAC_C
Cuts at: 2016 2016
Size: 5000
AclI AA'CG_TT
Cuts at: 4840 4840
Size: 5000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1029 2825 3257 4004 4272 655
Size: 374 1796 432 747 268 1383
Fragments arranged by size:
1796 1383 747 432 374 268
AfeI AGC'GCT
Cuts at: 424 436 424
Size: 12 4988
AflIII A'CryG_T
Cuts at: 992 2352 3456 4433 992
Size: 1360 1104 977 1559
Fragments arranged by size:
1559 1360 1104 977
AgeI A'CCGG_T
Cuts at: 3388 3388
Size: 5000
AhdI GACnn_n'nnGTC
Cuts at: 996 1901 996
Size: 905 4095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 796 1886 796
Size: 1090 3910
AlwNI CAG_nnn'CTG
Cuts at: 896 1326 1644 1852 2305 3872 896
Size: 430 318 208 453 1567 2024
Fragments arranged by size:
2024 1567 453 430 318 208
ApaI G_GGCC'C
Cuts at: 155 321 1216 1265 1435 1585 1592 1728 155
Size: 166 895 49 170 150 7 136 3427
Fragments arranged by size:
3427 895 170 166 150 136 49 7
ApaLI G'TGCA_C
Cuts at: 3770 3770
Size: 5000
ApoI r'AATT_y
Cuts at: 2004 4353 4609 2004
Size: 2349 256 2395
Fragments arranged by size:
2395 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5000
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
AvaI C'yCGr_G
Cuts at: 1216 1264 2020 1216
Size: 48 756 4196
Fragments arranged by size:
4196 756 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1740 1773 1740
Size: 33 4967
BamHI G'GATC_C
Cuts at: 2025 2025
Size: 5000
BanI G'GyrC_C
Cuts at: 327 1125 2016 2430 2678 2713 327
Size: 798 891 414 248 35 2614
Fragments arranged by size:
2614 891 798 414 248 35
BanII G_rGCy'C
Cuts at: 155 321 806 1040 1216 1265 1355 1383 1435
Size: 166 485 234 176 49 90 28 52
Cuts at: 1435 1585 1592 1728 2014 3044 155
Size: 150 7 136 286 1030 2111
Fragments arranged by size:
2111 1030 485 286 234 176 166 150
136 90 52 49 28 7
BbeI G_GCGC'C
Cuts at: 331 1129 2434 2682 331
Size: 798 1305 248 2649
Fragments arranged by size:
2649 1305 798 248
BbvCI CC'TCA_GC
Cuts at: 231 1875 231
Size: 1644 3356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1973 2007 1973
Size: 34 4966
BciVI GTATCCnnnnn_n'
Cuts at: 2898 3659 5000 2898
Size: 761 1341 2898
Fragments arranged by size:
2898 1341 761
BclI T'GATC_A
Cuts at: 495 2520 495
Size: 2025 2975
BglI GCCn_nnn'nGGC
Cuts at: 201 325 905 1417 1439 1647 1814 201
Size: 124 580 512 22 208 167 3387
Fragments arranged by size:
3387 580 512 208 167 124 22
BglII A'GATC_T
Cuts at: 2515 4552 2515
Size: 2037 2963
BlpI GC'TnA_GC
Cuts at: 805 2112 805
Size: 1307 3693
Bme1580I G_kGCm'C
Cuts at: 155 321 970 1216 1265 1435 1585 1592 1673
Size: 166 649 246 49 170 150 7 81
Cuts at: 1673 1728 2625 2718 3774 155
Size: 55 897 93 1056 1381
Fragments arranged by size:
1381 1056 897 649 246 170 166 150
93 81 55 49 7
BmrI ACTGGGnnnn_n'
Cuts at: 1722 2212 2293 2628 4772 1722
Size: 490 81 335 2144 1950
Fragments arranged by size:
2144 1950 490 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1057 3367 1057
Size: 2310 2690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 604 1363 1494 1509 1521 1538 1919 451
Size: 153 759 131 15 12 17 381 3532
Fragments arranged by size:
3532 759 381 153 131 17 15 12
Bpu10I CC'TnA_GC
Cuts at: 231 800 1875 231
Size: 569 1075 3356
Fragments arranged by size:
3356 1075 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2176 3055 3547 3845 811
Size: 1365 879 492 298 1966
Fragments arranged by size:
1966 1365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4704 4704
Size: 5000
BsaAI yAC'GTr
Cuts at: 2353 2353
Size: 5000
BsaBI GATnn'nnATC
Cuts at: 6 2514 4551 6
Size: 2508 2037 455
Fragments arranged by size:
2508 2037 455
BsaHI Gr'CG_yC
Cuts at: 328 731 1126 2431 2679 3381 4877 328
Size: 403 395 1305 248 702 1496 451
Fragments arranged by size:
1496 1305 702 451 403 395 248
BsaWI w'CCGG_w
Cuts at: 653 1030 1547 1883 2189 2710 3388 3662 3809
Size: 377 517 336 306 521 678 274 147
Cuts at: 3809 4220 653
Size: 411 1433
Fragments arranged by size:
1433 678 521 517 411 377 336 306
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 601 631 601
Size: 30 4970
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1524 1524
Size: 5000
BseYI C'CCAG_C
Cuts at: 320 1092 1242 1637 1739 1845 1859 2425 3183
Size: 772 150 395 102 106 14 566 758
Cuts at: 3183 3760 4256 320
Size: 577 496 1064
Fragments arranged by size:
1064 772 758 577 566 496 395 150
106 102 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 1270 756
Size: 514 4486
BsiEI CG_ry'CG
Cuts at: 104 1109 1412 2542 2588 3796 104
Size: 1005 303 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 1005 303 46
BsiHKAI G_wGCw'C
Cuts at: 164 806 962 1981 2014 2792 2982 3774 164
Size: 642 156 1019 33 778 190 792 1390
Fragments arranged by size:
1390 1019 792 778 642 190 156 33
BsmBI CGTCTCn'nnnn_
Cuts at: 738 738
Size: 5000
Bsp1286I G_dGCh'C
Cuts at: 155 164 321 806 962 970 1040 1216 1265
Size: 9 157 485 156 8 70 176 49
Cuts at: 1265 1355 1383 1435 1585 1592 1673 1728 1981
Size: 90 28 52 150 7 81 55 253
Cuts at: 1981 2014 2625 2718 2792 2982 3044 3774 155
Size: 33 611 93 74 190 62 730 1381
Fragments arranged by size:
1381 730 611 485 253 190 176 157
156 150 93 90 81 74 70 62
55 52 49 33 28 9 8 7
BspEI T'CCGG_A
Cuts at: 653 1883 4220 653
Size: 1230 2337 1433
Fragments arranged by size:
2337 1433 1230
BspHI T'CATG_A
Cuts at: 4176 4176
Size: 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 159 1486 2050 2264 2566 2947 159
Size: 1327 564 214 302 381 2212
Fragments arranged by size:
2212 1327 564 381 302 214
BsrBI CCG'CTC
Cuts at: 50 947 3292 3346 4830 4995 50
Size: 897 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 897 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2912 2912
Size: 5000
BsrFI r'CCGG_y
Cuts at: 1104 1271 2998 3179 3388 1104
Size: 167 1727 181 209 2716
Fragments arranged by size:
2716 1727 209 181 167
BssSI C'ACGA_G
Cuts at: 3271 3629 3271
Size: 358 4642
BstAPI GCAn_nnn'nTGC
Cuts at: 174 896 1239 2377 174
Size: 722 343 1138 2797
Fragments arranged by size:
2797 1138 722 343
BstBI TT'CG_AA
Cuts at: 3361 3361
Size: 5000
BstYI r'GATC_y
Cuts at: 1 914 2025 2492 2515 2850 3096 4108 4194
Size: 913 1111 467 23 335 246 1012 86
Cuts at: 4194 4206 4552 1
Size: 12 346 449
Fragments arranged by size:
1111 1012 913 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 294 294
Size: 5000
Bsu36I CC'TnA_GG
Cuts at: 953 953
Size: 5000
BtgI C'CryG_G
Cuts at: 107 126 375 726 1962 107
Size: 19 249 351 1236 3145
Fragments arranged by size:
3145 1236 351 249 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2950 3131 3294 2950
Size: 181 163 4656
Fragments arranged by size:
4656 181 163
BtsI GCAGTG_nn'
Cuts at: 420 778 816 1452 1708 2243 420
Size: 358 38 636 256 535 3177
Fragments arranged by size:
3177 636 535 358 256 38
DraI TTT'AAA
Cuts at: 1999 1999
Size: 5000
DrdI GACnn_nn'nnGTC
Cuts at: 2706 3564 2706
Size: 858 4142
EaeI y'GGCC_r
Cuts at: 110 474 518 791 906 983 1106 1409 2585
Size: 364 44 273 115 77 123 303 1176
Cuts at: 2585 2759 3150 3177 4522 110
Size: 174 391 27 1345 588
Fragments arranged by size:
1345 1176 588 391 364 303 273 174
123 115 77 44 27
EagI C'GGCC_G
Cuts at: 1106 1409 2585 1106
Size: 303 1176 3521
Fragments arranged by size:
3521 1176 303
EarI CTCTTCn'nnn_
Cuts at: 1048 1491 3023 3233 1048
Size: 443 1532 210 2815
Fragments arranged by size:
2815 1532 443 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1390 3208 3518 3664 4810 1390
Size: 1818 310 146 1146 1580
Fragments arranged by size:
1818 1580 1146 310 146
EcoICRI GAG'CTC
Cuts at: 804 2012 804
Size: 1208 3792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 604 655 1029 1363 1494 1509 1521 1538
Size: 153 51 374 334 131 15 12 17
Cuts at: 1538 1919 2825 3257 4004 4272 451
Size: 381 906 432 747 268 1179
Fragments arranged by size:
1179 906 747 432 381 374 334 268
153 131 51 17 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 556 556
Size: 5000
EcoO109I rG'GnC_Cy
Cuts at: 151 152 366 1212 1213 1323 1581 1582 1589
Size: 1 214 846 1 110 258 1 7
Cuts at: 1589 1626 1724 1945 2139 151
Size: 37 98 221 194 3012
Fragments arranged by size:
3012 846 258 221 214 194 110 98
37 7 1 1 1
EcoRI G'AATT_C
Cuts at: 2004 2004
Size: 5000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 660 3019 660
Size: 2359 2641
FspI TGC'GCA
Cuts at: 412 712 1336 2781 412
Size: 300 624 1445 2631
Fragments arranged by size:
2631 1445 624 300
HaeII r_GCGC'y
Cuts at: 331 426 438 869 1129 1852 2434 2682 3704
Size: 95 12 431 260 723 582 248 1022
Cuts at: 3704 4683 331
Size: 979 648
Fragments arranged by size:
1022 979 723 648 582 431 260 248
95 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 670 702 982 1014 1887 1919 2842
Size: 32 424 32 280 32 873 32 923
Cuts at: 2842 2872 2874 2904 214
Size: 30 2 30 2310
Fragments arranged by size:
2310 923 873 424 280 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1065 2039 1065
Size: 974 4026
KasI G'GCGC_C
Cuts at: 327 1125 2430 2678 327
Size: 798 1305 248 2649
Fragments arranged by size:
2649 1305 798 248
KpnI G_GTAC'C
Cuts at: 2020 2020
Size: 5000
MluI A'CGCG_T
Cuts at: 4433 4433
Size: 5000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3671 3855 3671
Size: 184 4816
MscI TGG'CCA
Cuts at: 476 520 793 908 985 2761 476
Size: 44 273 115 77 1776 2715
Fragments arranged by size:
2715 1776 273 115 77 44
MslI CAynn'nnrTG
Cuts at: 284 380 610 991 1015 2989 3116 284
Size: 96 230 381 24 1974 127 2168
Fragments arranged by size:
2168 1974 381 230 127 96 24
MspA1I CmG'CkG
Cuts at: 240 728 1644 2111 2425 2785 3798 4043 240
Size: 488 916 467 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 916 488 467 360 314 245
NaeI GCC'GGC
Cuts at: 1273 3181 1273
Size: 1908 3092
NarI GG'CG_CC
Cuts at: 328 1126 2431 2679 328
Size: 798 1305 248 2649
Fragments arranged by size:
2649 1305 798 248
NcoI C'CATG_G
Cuts at: 107 126 375 107
Size: 19 249 4732
Fragments arranged by size:
4732 249 19
NgoMIV G'CCGG_C
Cuts at: 1271 3179 1271
Size: 1908 3092
NspI r_CATG'y
Cuts at: 996 1341 2053 2553 3460 996
Size: 345 712 500 907 2536
Fragments arranged by size:
2536 907 712 500 345
PasI CC'CwG_GG
Cuts at: 148 1002 1374 1585 1652 148
Size: 854 372 211 67 3496
Fragments arranged by size:
3496 854 372 211 67
PciI A'CATG_T
Cuts at: 992 3456 992
Size: 2464 2536
PflMI CCAn_nnn'nTGG
Cuts at: 1326 1326
Size: 5000
PfoI T'CCnGG_A
Cuts at: 218 1319 218
Size: 1101 3899
PmeI GTTT'AAAC
Cuts at: 1999 1999
Size: 5000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 4968
PpuMI rG'GwC_Cy
Cuts at: 366 1323 1626 366
Size: 957 303 3740
Fragments arranged by size:
3740 957 303
PspOMI G'GGCC_C
Cuts at: 151 317 1212 1261 1431 1581 1588 1724 151
Size: 166 895 49 170 150 7 136 3427
Fragments arranged by size:
3427 895 170 166 150 136 49 7
PstI C_TGCA'G
Cuts at: 1235 1388 2047 2732 1235
Size: 153 659 685 3503
Fragments arranged by size:
3503 685 659 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5000
PvuII CAG'CTG
Cuts at: 2425 2785 2425
Size: 360 4640
RsrII CG'GwC_CG
Cuts at: 3195 3195
Size: 5000
SacI G_AGCT'C
Cuts at: 806 2014 806
Size: 1208 3792
SacII CC_GC'GG
Cuts at: 729 729
Size: 5000
SalI G'TCGA_C
Cuts at: 1063 2037 1063
Size: 974 4026
SapI GCTCTTCn'nnn_
Cuts at: 3023 3233 3023
Size: 210 4790
SbfI CC_TGCA'GG
Cuts at: 2047 2047
Size: 5000
ScaI AGT'ACT
Cuts at: 619 1144 4517 619
Size: 525 3373 1102
Fragments arranged by size:
3373 1102 525
SexAI A'CCwGG_T
Cuts at: 1325 1325
Size: 5000
SfcI C'TryA_G
Cuts at: 33 1231 1384 1560 2043 2728 3721 3912 33
Size: 1198 153 176 483 685 993 191 1121
Fragments arranged by size:
1198 1121 993 685 483 191 176 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 1417 1417
Size: 5000
SfoI GGC'GCC
Cuts at: 329 1127 2432 2680 329
Size: 798 1305 248 2649
Fragments arranged by size:
2649 1305 798 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
SmaI CCC'GGG
Cuts at: 1218 1266 2022 1218
Size: 48 756 4196
Fragments arranged by size:
4196 756 48
SmlI C'TyrA_G
Cuts at: 826 2155 3070 3562 3824 826
Size: 1329 915 492 262 2002
Fragments arranged by size:
2002 1329 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5000
SphI G_CATG'C
Cuts at: 1341 2053 2553 1341
Size: 712 500 3788
Fragments arranged by size:
3788 712 500
StyI C'CwwG_G
Cuts at: 107 126 375 1130 1752 2134 2488 107
Size: 19 249 755 622 382 354 2619
Fragments arranged by size:
2619 755 622 382 354 249 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 733 1859 2583 2716 3175 3384 733
Size: 1126 724 133 459 209 2349
Fragments arranged by size:
2349 1126 724 459 209 133
TatI w'GTAC_w
Cuts at: 617 627 1142 4515 617
Size: 10 515 3373 1102
Fragments arranged by size:
3373 1102 515 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4517 4549 4517
Size: 32 4968
Tth111I GACn'n_nGTC
Cuts at: 2797 2797
Size: 5000
XbaI T'CTAG_A
Cuts at: 2031 2031
Size: 5000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 2495 876
Size: 1619 3381
XmaI C'CCGG_G
Cuts at: 1216 1264 2020 1216
Size: 48 756 4196
Fragments arranged by size:
4196 756 48
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BbsI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstXI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SanDI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI