Restriction map for
pF1KB3981
(Circular) (Six-base) MAPSORT of: pF1KB3981.seq Check: 6407 from: 1 to: 3659
pF1KB3981 3659 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 697 697
Size: 3659
Acc65I G'GTAC_C
Cuts at: 675 675
Size: 3659
AclI AA'CG_TT
Cuts at: 3499 3499
Size: 3659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 245 1484 1916 2663 2931 229
Size: 16 1239 432 747 268 957
Fragments arranged by size:
1239 957 747 432 268 16
AflIII A'CryG_T
Cuts at: 1011 2115 3092 1011
Size: 1104 977 1578
Fragments arranged by size:
1578 1104 977
AgeI A'CCGG_T
Cuts at: 2047 2047
Size: 3659
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 587 619 587
Size: 32 3627
AlwNI CAG_nnn'CTG
Cuts at: 511 964 2531 511
Size: 453 1567 1639
Fragments arranged by size:
1639 1567 453
ApaLI G'TGCA_C
Cuts at: 143 2429 143
Size: 2286 1373
ApoI r'AATT_y
Cuts at: 663 3012 3268 663
Size: 2349 256 1054
Fragments arranged by size:
2349 1054 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3659
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
AvaI C'yCGr_G
Cuts at: 679 679
Size: 3659
BamHI G'GATC_C
Cuts at: 684 684
Size: 3659
BanI G'GyrC_C
Cuts at: 675 1089 1337 1372 675
Size: 414 248 35 2962
Fragments arranged by size:
2962 414 248 35
BanII G_rGCy'C
Cuts at: 673 1703 673
Size: 1030 2629
BbeI G_GCGC'C
Cuts at: 1093 1341 1093
Size: 248 3411
BbsI GAAGACnn'nnnn_
Cuts at: 233 233
Size: 3659
BciVI GTATCCnnnnn_n'
Cuts at: 1557 2318 3659 1557
Size: 761 1341 1557
Fragments arranged by size:
1557 1341 761
BclI T'GATC_A
Cuts at: 1179 1179
Size: 3659
BglII A'GATC_T
Cuts at: 1174 3211 1174
Size: 2037 1622
BlpI GC'TnA_GC
Cuts at: 582 771 582
Size: 189 3470
Bme1580I G_kGCm'C
Cuts at: 147 1284 1377 2433 147
Size: 1137 93 1056 1373
Fragments arranged by size:
1373 1137 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 630 871 952 1287 3431 630
Size: 241 81 335 2144 858
Fragments arranged by size:
2144 858 335 241 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 3659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 460
Size: 3659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1714 2206 2504 835
Size: 879 492 298 1990
Fragments arranged by size:
1990 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3363 3363
Size: 3659
BsaAI yAC'GTr
Cuts at: 1012 1012
Size: 3659
BsaBI GATnn'nnATC
Cuts at: 6 1173 3210 6
Size: 1167 2037 455
Fragments arranged by size:
2037 1167 455
BsaHI Gr'CG_yC
Cuts at: 1090 1338 2040 3536 1090
Size: 248 702 1496 1213
Fragments arranged by size:
1496 1213 702 248
BsaWI w'CCGG_w
Cuts at: 848 1369 2047 2321 2468 2879 848
Size: 521 678 274 147 411 1628
Fragments arranged by size:
1628 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 587 617 587
Size: 30 3629
BseYI C'CCAG_C
Cuts at: 1084 1842 2419 2915 1084
Size: 758 577 496 1828
Fragments arranged by size:
1828 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 410
Size: 3659
BsiEI CG_ry'CG
Cuts at: 104 558 1201 1247 2455 104
Size: 454 643 46 1208 1308
Fragments arranged by size:
1308 1208 643 454 46
BsiHKAI G_wGCw'C
Cuts at: 147 473 673 1451 1641 2433 147
Size: 326 200 778 190 792 1373
Fragments arranged by size:
1373 792 778 326 200 190
BsmBI CGTCTCn'nnnn_
Cuts at: 406 406
Size: 3659
Bsp1286I G_dGCh'C
Cuts at: 147 473 673 1284 1377 1451 1641 1703 2433
Size: 326 200 611 93 74 190 62 730
Cuts at: 2433 147
Size: 1373
Fragments arranged by size:
1373 730 611 326 200 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2879 2879
Size: 3659
BspHI T'CATG_A
Cuts at: 2835 2835
Size: 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 548 709 923 1225 1606 548
Size: 161 214 302 381 2601
Fragments arranged by size:
2601 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 1951 2005 3489 3654 50
Size: 1901 54 1484 165 55
Fragments arranged by size:
1901 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1571 1571
Size: 3659
BsrFI r'CCGG_y
Cuts at: 1657 1838 2047 1657
Size: 181 209 3269
Fragments arranged by size:
3269 209 181
BsrGI T'GTAC_A
Cuts at: 273 273
Size: 3659
BssSI C'ACGA_G
Cuts at: 1930 2288 1930
Size: 358 3301
BstAPI GCAn_nnn'nTGC
Cuts at: 495 511 1036 495
Size: 16 525 3118
Fragments arranged by size:
3118 525 16
BstBI TT'CG_AA
Cuts at: 2020 2020
Size: 3659
BstYI r'GATC_y
Cuts at: 1 569 684 1151 1174 1509 1755 2767 2853
Size: 568 115 467 23 335 246 1012 86
Cuts at: 2853 2865 3211 1
Size: 12 346 449
Fragments arranged by size:
1012 568 467 449 346 335 246 115
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3659
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1609 1790 1953 1609
Size: 181 163 3315
Fragments arranged by size:
3315 181 163
BtsI GCAGTG_nn'
Cuts at: 902 902
Size: 3659
DraI TTT'AAA
Cuts at: 291 658 291
Size: 367 3292
DrdI GACnn_nn'nnGTC
Cuts at: 1365 2223 1365
Size: 858 2801
EaeI y'GGCC_r
Cuts at: 1244 1418 1809 1836 3181 1244
Size: 174 391 27 1345 1722
Fragments arranged by size:
1722 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1244 1244
Size: 3659
EarI CTCTTCn'nnn_
Cuts at: 209 404 1682 1892 209
Size: 195 1278 210 1976
Fragments arranged by size:
1976 1278 210 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1867 2177 2323 3469 1867
Size: 310 146 1146 2057
Fragments arranged by size:
2057 1146 310 146
EcoICRI GAG'CTC
Cuts at: 671 671
Size: 3659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 245 460 1484 1916 2663 2931 229
Size: 16 215 1024 432 747 268 957
Fragments arranged by size:
1024 957 747 432 268 215 16
EcoO109I rG'GnC_Cy
Cuts at: 798 798
Size: 3659
EcoRI G'AATT_C
Cuts at: 663 663
Size: 3659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1678 1678
Size: 3659
FspI TGC'GCA
Cuts at: 130 1440 130
Size: 1310 2349
HaeII r_GCGC'y
Cuts at: 1093 1341 2363 3342 1093
Size: 248 1022 979 1410
Fragments arranged by size:
1410 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1501 1531 1533 1563 1501
Size: 30 2 30 3597
Fragments arranged by size:
3597 30 30 2
HincII GTy'rAC
Cuts at: 698 698
Size: 3659
KasI G'GCGC_C
Cuts at: 1089 1337 1089
Size: 248 3411
KpnI G_GTAC'C
Cuts at: 679 679
Size: 3659
MluI A'CGCG_T
Cuts at: 3092 3092
Size: 3659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2514 2330
Size: 184 3475
MscI TGG'CCA
Cuts at: 1420 1420
Size: 3659
MslI CAynn'nnrTG
Cuts at: 1648 1775 1648
Size: 127 3532
MspA1I CmG'CkG
Cuts at: 770 1084 1444 2457 2702 770
Size: 314 360 1013 245 1727
Fragments arranged by size:
1727 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1840 1840
Size: 3659
NarI GG'CG_CC
Cuts at: 1090 1338 1090
Size: 248 3411
NcoI C'CATG_G
Cuts at: 107 107
Size: 3659
NgoMIV G'CCGG_C
Cuts at: 1838 1838
Size: 3659
NspI r_CATG'y
Cuts at: 712 1212 2119 712
Size: 500 907 2252
Fragments arranged by size:
2252 907 500
PciI A'CATG_T
Cuts at: 2115 2115
Size: 3659
PmeI GTTT'AAAC
Cuts at: 658 658
Size: 3659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 587 619 2798 2830 587
Size: 32 2179 32 1416
Fragments arranged by size:
2179 1416 32 32
PstI C_TGCA'G
Cuts at: 706 1391 706
Size: 685 2974
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3659
PvuII CAG'CTG
Cuts at: 1084 1444 1084
Size: 360 3299
RsrII CG'GwC_CG
Cuts at: 1854 1854
Size: 3659
SacI G_AGCT'C
Cuts at: 673 673
Size: 3659
SalI G'TCGA_C
Cuts at: 696 696
Size: 3659
SapI GCTCTTCn'nnn_
Cuts at: 1682 1892 1682
Size: 210 3449
SbfI CC_TGCA'GG
Cuts at: 706 706
Size: 3659
ScaI AGT'ACT
Cuts at: 3176 3176
Size: 3659
SfcI C'TryA_G
Cuts at: 33 702 1387 2380 2571 33
Size: 669 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 191
SfoI GGC'GCC
Cuts at: 1091 1339 1091
Size: 248 3411
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
SmaI CCC'GGG
Cuts at: 681 681
Size: 3659
SmlI C'TyrA_G
Cuts at: 814 1729 2221 2483 814
Size: 915 492 262 1990
Fragments arranged by size:
1990 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3659
SphI G_CATG'C
Cuts at: 712 1212 712
Size: 500 3159
StyI C'CwwG_G
Cuts at: 107 793 1147 107
Size: 686 354 2619
Fragments arranged by size:
2619 686 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 572 1242 1375 1834 2043 572
Size: 670 133 459 209 2188
Fragments arranged by size:
2188 670 459 209 133
TatI w'GTAC_w
Cuts at: 273 3174 273
Size: 2901 758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 3627
Tth111I GACn'n_nGTC
Cuts at: 559 1456 559
Size: 897 2762
XbaI T'CTAG_A
Cuts at: 690 690
Size: 3659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1154 1154
Size: 3659
XmaI C'CCGG_G
Cuts at: 679 679
Size: 3659
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI