Restriction map for
pF1KB3988
(Circular) (Six-base) MAPSORT of: pF1KB3988.seq Check: 6338 from: 1 to: 3632
pF1KB3988 3632 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 670 670
Size: 3632
Acc65I G'GTAC_C
Cuts at: 648 648
Size: 3632
AclI AA'CG_TT
Cuts at: 3472 3472
Size: 3632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1889 2636 2904 1457
Size: 432 747 268 2185
Fragments arranged by size:
2185 747 432 268
AflII C'TTAA_G
Cuts at: 217 217
Size: 3632
AflIII A'CryG_T
Cuts at: 984 2088 3065 984
Size: 1104 977 1551
Fragments arranged by size:
1551 1104 977
AgeI A'CCGG_T
Cuts at: 2020 2020
Size: 3632
AlwNI CAG_nnn'CTG
Cuts at: 133 937 2504 133
Size: 804 1567 1261
Fragments arranged by size:
1567 1261 804
ApaLI G'TGCA_C
Cuts at: 326 2402 326
Size: 2076 1556
ApoI r'AATT_y
Cuts at: 202 636 2985 3241 202
Size: 434 2349 256 593
Fragments arranged by size:
2349 593 434 256
AseI AT'TA_AT
Cuts at: 20 463 20
Size: 443 3189
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
AvaI C'yCGr_G
Cuts at: 652 652
Size: 3632
BamHI G'GATC_C
Cuts at: 657 657
Size: 3632
BanI G'GyrC_C
Cuts at: 648 1062 1310 1345 648
Size: 414 248 35 2935
Fragments arranged by size:
2935 414 248 35
BanII G_rGCy'C
Cuts at: 441 646 1676 441
Size: 205 1030 2397
Fragments arranged by size:
2397 1030 205
BbeI G_GCGC'C
Cuts at: 1066 1314 1066
Size: 248 3384
BciVI GTATCCnnnnn_n'
Cuts at: 304 1530 2291 3632 304
Size: 1226 761 1341 304
Fragments arranged by size:
1341 1226 761 304
BclI T'GATC_A
Cuts at: 1152 1152
Size: 3632
BglII A'GATC_T
Cuts at: 1147 3184 1147
Size: 2037 1595
BlpI GC'TnA_GC
Cuts at: 744 744
Size: 3632
Bme1580I G_kGCm'C
Cuts at: 330 1257 1350 2406 330
Size: 927 93 1056 1556
Fragments arranged by size:
1556 1056 927 93
BmrI ACTGGGnnnn_n'
Cuts at: 844 925 1260 3404 844
Size: 81 335 2144 1072
Fragments arranged by size:
2144 1072 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1687 2179 2477 808
Size: 879 492 298 1963
Fragments arranged by size:
1963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3336 3336
Size: 3632
BsaAI yAC'GTr
Cuts at: 985 985
Size: 3632
BsaBI GATnn'nnATC
Cuts at: 6 1146 3183 6
Size: 1140 2037 455
Fragments arranged by size:
2037 1140 455
BsaHI Gr'CG_yC
Cuts at: 1063 1311 2013 3509 1063
Size: 248 702 1496 1186
Fragments arranged by size:
1496 1186 702 248
BsaWI w'CCGG_w
Cuts at: 821 1342 2020 2294 2441 2852 821
Size: 521 678 274 147 411 1601
Fragments arranged by size:
1601 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 126 1057 1815 2392 2888 126
Size: 931 758 577 496 870
Fragments arranged by size:
931 870 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1174 1220 2428 104
Size: 1070 46 1208 1308
Fragments arranged by size:
1308 1208 1070 46
BsiHKAI G_wGCw'C
Cuts at: 330 441 646 1424 1614 2406 330
Size: 111 205 778 190 792 1556
Fragments arranged by size:
1556 792 778 205 190 111
BsmI GAATG_Cn'
Cuts at: 620 620
Size: 3632
Bsp1286I G_dGCh'C
Cuts at: 330 441 646 1257 1350 1424 1614 1676 2406
Size: 111 205 611 93 74 190 62 730
Cuts at: 2406 330
Size: 1556
Fragments arranged by size:
1556 730 611 205 190 111 93 74
62
BspEI T'CCGG_A
Cuts at: 2852 2852
Size: 3632
BspHI T'CATG_A
Cuts at: 2808 2808
Size: 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 682 896 1198 1579 682
Size: 214 302 381 2735
Fragments arranged by size:
2735 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1924 1978 3462 3627 50
Size: 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 414 1544 414
Size: 1130 2502
BsrFI r'CCGG_y
Cuts at: 1630 1811 2020 1630
Size: 181 209 3242
Fragments arranged by size:
3242 209 181
BssSI C'ACGA_G
Cuts at: 1903 2261 1903
Size: 358 3274
BstAPI GCAn_nnn'nTGC
Cuts at: 1009 1009
Size: 3632
BstBI TT'CG_AA
Cuts at: 1993 1993
Size: 3632
BstYI r'GATC_y
Cuts at: 1 657 1124 1147 1482 1728 2740 2826 2838
Size: 656 467 23 335 246 1012 86 12
Cuts at: 2838 3184 1
Size: 346 449
Fragments arranged by size:
1012 656 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1582 1763 1926 1582
Size: 181 163 3288
Fragments arranged by size:
3288 181 163
BtsI GCAGTG_nn'
Cuts at: 875 875
Size: 3632
DraI TTT'AAA
Cuts at: 631 631
Size: 3632
DrdI GACnn_nn'nnGTC
Cuts at: 1338 2196 1338
Size: 858 2774
EaeI y'GGCC_r
Cuts at: 1217 1391 1782 1809 3154 1217
Size: 174 391 27 1345 1695
Fragments arranged by size:
1695 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1217 1217
Size: 3632
EarI CTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1840 2150 2296 3442 1840
Size: 310 146 1146 2030
Fragments arranged by size:
2030 1146 310 146
EcoICRI GAG'CTC
Cuts at: 439 644 439
Size: 205 3427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1457 1889 2636 2904 425
Size: 1032 432 747 268 1153
Fragments arranged by size:
1153 1032 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 771 771
Size: 3632
EcoRI G'AATT_C
Cuts at: 636 636
Size: 3632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1651 1651
Size: 3632
FspI TGC'GCA
Cuts at: 1413 1413
Size: 3632
HaeII r_GCGC'y
Cuts at: 1066 1314 2336 3315 1066
Size: 248 1022 979 1383
Fragments arranged by size:
1383 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1474 1504 1506 1536 1474
Size: 30 2 30 3570
Fragments arranged by size:
3570 30 30 2
HincII GTy'rAC
Cuts at: 671 671
Size: 3632
KasI G'GCGC_C
Cuts at: 1062 1310 1062
Size: 248 3384
KpnI G_GTAC'C
Cuts at: 652 652
Size: 3632
MluI A'CGCG_T
Cuts at: 3065 3065
Size: 3632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 205 362 620 2303 2487 205
Size: 157 258 1683 184 1350
Fragments arranged by size:
1683 1350 258 184 157
MscI TGG'CCA
Cuts at: 1393 1393
Size: 3632
MslI CAynn'nnrTG
Cuts at: 1621 1748 1621
Size: 127 3505
MspA1I CmG'CkG
Cuts at: 743 1057 1417 2430 2675 743
Size: 314 360 1013 245 1700
Fragments arranged by size:
1700 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1813 1813
Size: 3632
NarI GG'CG_CC
Cuts at: 1063 1311 1063
Size: 248 3384
NcoI C'CATG_G
Cuts at: 107 107
Size: 3632
NgoMIV G'CCGG_C
Cuts at: 1811 1811
Size: 3632
NspI r_CATG'y
Cuts at: 685 1185 2092 685
Size: 500 907 2225
Fragments arranged by size:
2225 907 500
PciI A'CATG_T
Cuts at: 2088 2088
Size: 3632
PmeI GTTT'AAAC
Cuts at: 631 631
Size: 3632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 3600
PstI C_TGCA'G
Cuts at: 679 1364 679
Size: 685 2947
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3632
PvuII CAG'CTG
Cuts at: 1057 1417 1057
Size: 360 3272
RsrII CG'GwC_CG
Cuts at: 1827 1827
Size: 3632
SacI G_AGCT'C
Cuts at: 441 646 441
Size: 205 3427
SalI G'TCGA_C
Cuts at: 669 669
Size: 3632
SapI GCTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
SbfI CC_TGCA'GG
Cuts at: 679 679
Size: 3632
ScaI AGT'ACT
Cuts at: 446 3149 446
Size: 2703 929
SexAI A'CCwGG_T
Cuts at: 393 393
Size: 3632
SfcI C'TryA_G
Cuts at: 33 675 1360 2353 2544 33
Size: 642 685 993 191 1121
Fragments arranged by size:
1121 993 685 642 191
SfoI GGC'GCC
Cuts at: 1064 1312 1064
Size: 248 3384
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
SmaI CCC'GGG
Cuts at: 654 654
Size: 3632
SmlI C'TyrA_G
Cuts at: 217 787 1702 2194 2456 217
Size: 570 915 492 262 1393
Fragments arranged by size:
1393 915 570 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3632
SphI G_CATG'C
Cuts at: 685 1185 685
Size: 500 3132
StuI AGG'CCT
Cuts at: 428 428
Size: 3632
StyI C'CwwG_G
Cuts at: 107 429 766 1120 107
Size: 322 337 354 2619
Fragments arranged by size:
2619 354 337 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1215 1348 1807 2016 1215
Size: 133 459 209 2831
Fragments arranged by size:
2831 459 209 133
TatI w'GTAC_w
Cuts at: 444 495 3147 444
Size: 51 2652 929
Fragments arranged by size:
2652 929 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3600
Tth111I GACn'n_nGTC
Cuts at: 1429 1429
Size: 3632
XbaI T'CTAG_A
Cuts at: 663 663
Size: 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1127 1127
Size: 3632
XmaI C'CCGG_G
Cuts at: 652 652
Size: 3632
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI