Restriction map for
pF1KB3990
(Circular) (Six-base) MAPSORT of: pF1KB3990.seq Check: 1261 from: 1 to: 6287
pF1KB3990 6287 bp
With 182 enzymes: SgfI *
October 29, 2009 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1752 2162 2299 1752
Size: 410 137 5740
Fragments arranged by size:
5740 410 137
AccI GT'mk_AC
Cuts at: 3325 3325
Size: 6287
Acc65I G'GTAC_C
Cuts at: 1893 3303 1893
Size: 1410 4877
AclI AA'CG_TT
Cuts at: 6127 6127
Size: 6287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 1906 2917 3043 4112 4544 5291 5559 1693
Size: 213 1011 126 1069 432 747 268 2421
Fragments arranged by size:
2421 1069 1011 747 432 268 213 126
AflII C'TTAA_G
Cuts at: 366 366
Size: 6287
AflIII A'CryG_T
Cuts at: 812 1165 3639 4743 5720 812
Size: 353 2474 1104 977 1379
Fragments arranged by size:
2474 1379 1104 977 353
AgeI A'CCGG_T
Cuts at: 4675 4675
Size: 6287
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2032 2064 2075 2107 2032
Size: 32 11 32 6212
Fragments arranged by size:
6212 32 32 11
AlwNI CAG_nnn'CTG
Cuts at: 735 1544 1689 2394 3592 5159 735
Size: 809 145 705 1198 1567 1863
Fragments arranged by size:
1863 1567 1198 809 705 145
ApaI G_GGCC'C
Cuts at: 906 1578 906
Size: 672 5615
ApaLI G'TGCA_C
Cuts at: 829 5057 829
Size: 4228 2059
ApoI r'AATT_y
Cuts at: 3180 3291 5640 5896 3180
Size: 111 2349 256 3571
Fragments arranged by size:
3571 2349 256 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 6287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
AvaI C'yCGr_G
Cuts at: 862 2142 2703 3307 862
Size: 1280 561 604 3842
Fragments arranged by size:
3842 1280 604 561
BamHI G'GATC_C
Cuts at: 998 3312 998
Size: 2314 3973
BanI G'GyrC_C
Cuts at: 849 943 1114 1228 1893 2175 3303 3717 3965
Size: 94 171 114 665 282 1128 414 248
Cuts at: 3965 4000 849
Size: 35 3136
Fragments arranged by size:
3136 1128 665 414 282 248 171 114
94 35
BanII G_rGCy'C
Cuts at: 467 906 1040 1205 1354 1427 1536 1578 2238
Size: 439 134 165 149 73 109 42 660
Cuts at: 2238 3301 4331 467
Size: 1063 1030 2423
Fragments arranged by size:
2423 1063 1030 660 439 165 149 134
109 73 42
BbeI G_GCGC'C
Cuts at: 3721 3969 3721
Size: 248 6039
BbsI GAAGACnn'nnnn_
Cuts at: 2207 2207
Size: 6287
BciVI GTATCCnnnnn_n'
Cuts at: 4185 4946 6287 4185
Size: 761 1341 4185
Fragments arranged by size:
4185 1341 761
BclI T'GATC_A
Cuts at: 2945 3807 2945
Size: 862 5425
BglI GCCn_nnn'nGGC
Cuts at: 1177 2163 2403 2818 1177
Size: 986 240 415 4646
Fragments arranged by size:
4646 986 415 240
BglII A'GATC_T
Cuts at: 3802 5839 3802
Size: 2037 4250
BlpI GC'TnA_GC
Cuts at: 1421 2371 3399 1421
Size: 950 1028 4309
Fragments arranged by size:
4309 1028 950
Bme1580I G_kGCm'C
Cuts at: 456 833 854 906 931 993 1173 1578 3912
Size: 377 21 52 25 62 180 405 2334
Cuts at: 3912 4005 5061 456
Size: 93 1056 1682
Fragments arranged by size:
2334 1682 1056 405 377 180 93 62
52 25 21
BmgBI CAC'GTC
Cuts at: 1166 2205 3018 1166
Size: 1039 813 4435
Fragments arranged by size:
4435 1039 813
BmrI ACTGGGnnnn_n'
Cuts at: 3499 3580 3915 6059 3499
Size: 81 335 2144 3727
Fragments arranged by size:
3727 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2802 4654 2802
Size: 1852 4435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 625 1049 1262 1723 2269 2664 2785 2908
Size: 327 424 213 461 546 395 121 123
Cuts at: 2908 298
Size: 3677
Fragments arranged by size:
3677 546 461 424 395 327 213 123
121
Bpu10I CC'TnA_GC
Cuts at: 2159 3235 2159
Size: 1076 5211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 293 1537 1735 1946 3463 4342 4834 5132
Size: 99 1244 198 211 1517 879 492 298
Cuts at: 5132 194
Size: 1349
Fragments arranged by size:
1517 1349 1244 879 492 298 211 198
99
BsaI GGTCTCn'nnnn_
Cuts at: 2578 5991 2578
Size: 3413 2874
BsaAI yAC'GTr
Cuts at: 981 3640 981
Size: 2659 3628
BsaBI GATnn'nnATC
Cuts at: 6 2000 3801 5838 6
Size: 1994 1801 2037 455
Fragments arranged by size:
2037 1994 1801 455
BsaHI Gr'CG_yC
Cuts at: 749 1334 2990 3718 3966 4668 6164 749
Size: 585 1656 728 248 702 1496 872
Fragments arranged by size:
1656 1496 872 728 702 585 248
BsaWI w'CCGG_w
Cuts at: 3191 3476 3997 4675 4949 5096 5507 3191
Size: 285 521 678 274 147 411 3971
Fragments arranged by size:
3971 678 521 411 285 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2075 2105 2075
Size: 30 6257
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 601 1660 2005 2266 2557 2613 2667 126
Size: 475 1059 345 261 291 56 54 3746
Fragments arranged by size:
3746 1059 475 345 291 261 56 54
BseYI C'CCAG_C
Cuts at: 421 971 2442 3712 4470 5047 5543 421
Size: 550 1471 1270 758 577 496 1165
Fragments arranged by size:
1471 1270 1165 758 577 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1269 2937 1269
Size: 1668 4619
BsiEI CG_ry'CG
Cuts at: 104 548 969 1445 3829 3875 5083 104
Size: 444 421 476 2384 46 1208 1308
Fragments arranged by size:
2384 1308 1208 476 444 421 46
BsiHKAI G_wGCw'C
Cuts at: 833 1354 2518 3038 3301 4079 4269 5061 833
Size: 521 1164 520 263 778 190 792 2059
Fragments arranged by size:
2059 1164 792 778 521 520 263 190
BsmI GAATG_Cn'
Cuts at: 1153 1153
Size: 6287
Bsp1286I G_dGCh'C
Cuts at: 456 467 833 854 906 931 993 1040 1173
Size: 11 366 21 52 25 62 47 133
Cuts at: 1173 1205 1354 1427 1536 1578 2238 2518 3038
Size: 32 149 73 109 42 660 280 520
Cuts at: 3038 3301 3912 4005 4079 4269 4331 5061 456
Size: 263 611 93 74 190 62 730 1682
Fragments arranged by size:
1682 730 660 611 520 366 280 263
190 149 133 109 93 74 73 62
62 52 47 42 32 25 21 11
BspEI T'CCGG_A
Cuts at: 3191 5507 3191
Size: 2316 3971
BspHI T'CATG_A
Cuts at: 5463 5463
Size: 6287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1752 1767 2162 2299 2560 3337 3551 3853 4234
Size: 15 395 137 261 777 214 302 381
Cuts at: 4234 1752
Size: 3805
Fragments arranged by size:
3805 777 395 381 302 261 214 137
15
BsrBI CCG'CTC
Cuts at: 50 351 2163 2694 4579 4633 6117 6282 50
Size: 301 1812 531 1885 54 1484 165 55
Fragments arranged by size:
1885 1812 1484 531 301 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4199 4199
Size: 6287
BsrFI r'CCGG_y
Cuts at: 438 2739 4285 4466 4675 438
Size: 2301 1546 181 209 2050
Fragments arranged by size:
2301 2050 1546 209 181
BsrGI T'GTAC_A
Cuts at: 1187 1187
Size: 6287
BssHII G'CGCG_C
Cuts at: 1707 1707
Size: 6287
BssSI C'ACGA_G
Cuts at: 4558 4916 4558
Size: 358 5929
BstAPI GCAn_nnn'nTGC
Cuts at: 1127 1689 3664 1127
Size: 562 1975 3750
Fragments arranged by size:
3750 1975 562
BstBI TT'CG_AA
Cuts at: 2423 4648 2423
Size: 2225 4062
BstXI CCAn_nnnn'nTGG
Cuts at: 1073 1180 1073
Size: 107 6180
BstYI r'GATC_y
Cuts at: 1 998 2858 3312 3779 3802 4137 4383 5395
Size: 997 1860 454 467 23 335 246 1012
Cuts at: 5395 5481 5493 5839 1
Size: 86 12 346 449
Fragments arranged by size:
1860 1012 997 467 454 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 429 1784 1925 2060 2179 2399 3246 429
Size: 1355 141 135 119 220 847 3470
Fragments arranged by size:
3470 1355 847 220 141 135 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4237 4418 4581 4237
Size: 181 163 5943
Fragments arranged by size:
5943 181 163
BtsI GCAGTG_nn'
Cuts at: 174 302 699 885 2781 3109 3530 174
Size: 128 397 186 1896 328 421 2931
Fragments arranged by size:
2931 1896 421 397 328 186 128
ClaI AT'CG_AT
Cuts at: 1043 1043
Size: 6287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 921 956 921
Size: 35 6252
DraI TTT'AAA
Cuts at: 3286 3286
Size: 6287
DraIII CAC_nnn'GTG
Cuts at: 984 984
Size: 6287
DrdI GACnn_nn'nnGTC
Cuts at: 3993 4851 3993
Size: 858 5429
EaeI y'GGCC_r
Cuts at: 446 545 824 1499 2819 2841 2976 3872 4046
Size: 99 279 675 1320 22 135 896 174
Cuts at: 4046 4437 4464 5809 446
Size: 391 27 1345 924
Fragments arranged by size:
1345 1320 924 896 675 391 279 174
135 99 27 22
EagI C'GGCC_G
Cuts at: 545 3872 545
Size: 3327 2960
EarI CTCTTCn'nnn_
Cuts at: 808 2129 2420 2492 2534 4310 4520 808
Size: 1321 291 72 42 1776 210 2575
Fragments arranged by size:
2575 1776 1321 291 210 72 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 489 4495 4805 4951 6097 489
Size: 4006 310 146 1146 679
Fragments arranged by size:
4006 1146 679 310 146
EcoICRI GAG'CTC
Cuts at: 1352 3299 1352
Size: 1947 4340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 625 1049 1262 1693 1723 1906 2269 2664
Size: 327 424 213 431 30 183 363 395
Cuts at: 2664 2785 2908 2917 3043 4112 4544 5291 5559
Size: 121 123 9 126 1069 432 747 268
Cuts at: 5559 298
Size: 1026
Fragments arranged by size:
1069 1026 747 432 431 424 395 363
327 268 213 183 126 123 121 30
9
EcoO109I rG'GnC_Cy
Cuts at: 414 1574 1575 1851 3426 414
Size: 1160 1 276 1575 3275
Fragments arranged by size:
3275 1575 1160 276 1
EcoRI G'AATT_C
Cuts at: 3180 3291 3180
Size: 111 6176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4306 4306
Size: 6287
FspI TGC'GCA
Cuts at: 475 1735 1871 4068 475
Size: 1260 136 2197 2694
Fragments arranged by size:
2694 2197 1260 136
FspAI rTGC'GCAy
Cuts at: 475 475
Size: 6287
HaeII r_GCGC'y
Cuts at: 249 428 2562 3721 3969 4991 5970 249
Size: 179 2134 1159 248 1022 979 566
Fragments arranged by size:
2134 1159 1022 979 566 248 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 192 224 679 711 715 747 1476 1508 4129
Size: 32 455 32 4 32 729 32 2621
Cuts at: 4129 4159 4161 4191 192
Size: 30 2 30 2288
Fragments arranged by size:
2621 2288 729 455 32 32 32 32
30 30 4 2
HincII GTy'rAC
Cuts at: 3326 3326
Size: 6287
KasI G'GCGC_C
Cuts at: 3717 3965 3717
Size: 248 6039
KpnI G_GTAC'C
Cuts at: 1897 3307 1897
Size: 1410 4877
MluI A'CGCG_T
Cuts at: 5720 5720
Size: 6287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 4958 5142 2459
Size: 2499 184 3604
Fragments arranged by size:
3604 2499 184
MscI TGG'CCA
Cuts at: 1501 2978 4048 1501
Size: 1477 1070 3740
Fragments arranged by size:
3740 1477 1070
MslI CAynn'nnrTG
Cuts at: 766 1195 4276 4403 766
Size: 429 3081 127 2650
Fragments arranged by size:
3081 2650 429 127
MspA1I CmG'CkG
Cuts at: 971 1509 1686 1806 1839 2062 2181 2259 2309
Size: 538 177 120 33 223 119 78 50
Cuts at: 2309 2921 3398 3712 4072 5085 5330 971
Size: 612 477 314 360 1013 245 1928
Fragments arranged by size:
1928 1013 612 538 477 360 314 245
223 177 120 119 78 50 33
NaeI GCC'GGC
Cuts at: 440 4468 440
Size: 4028 2259
NarI GG'CG_CC
Cuts at: 3718 3966 3718
Size: 248 6039
NcoI C'CATG_G
Cuts at: 1784 1925 1784
Size: 141 6146
NgoMIV G'CCGG_C
Cuts at: 438 4466 438
Size: 4028 2259
NspI r_CATG'y
Cuts at: 663 816 1088 2280 2522 2856 3042 3340 3840
Size: 153 272 1192 242 334 186 298 500
Cuts at: 3840 4747 663
Size: 907 2203
Fragments arranged by size:
2203 1192 907 500 334 298 272 242
186 153
PciI A'CATG_T
Cuts at: 812 4743 812
Size: 3931 2356
PfoI T'CCnGG_A
Cuts at: 458 1564 2314 458
Size: 1106 750 4431
Fragments arranged by size:
4431 1106 750
PmeI GTTT'AAAC
Cuts at: 3286 3286
Size: 6287
PmlI CAC'GTG
Cuts at: 981 981
Size: 6287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2075 2107 3049 3081 5426 5458 2075
Size: 32 942 32 2345 32 2904
Fragments arranged by size:
2904 2345 942 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1851 1851
Size: 6287
PshAI GACnn'nnGTC
Cuts at: 2570 2570
Size: 6287
PspOMI G'GGCC_C
Cuts at: 902 1574 902
Size: 672 5615
PspXI vC'TCGA_Gb
Cuts at: 2703 2703
Size: 6287
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 514 546 514
Size: 32 6255
PstI C_TGCA'G
Cuts at: 144 896 1598 1610 2495 3334 4019 144
Size: 752 702 12 885 839 685 2412
Fragments arranged by size:
2412 885 839 752 702 685 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6287
PvuII CAG'CTG
Cuts at: 1839 2259 3712 4072 1839
Size: 420 1453 360 4054
Fragments arranged by size:
4054 1453 420 360
RsrII CG'GwC_CG
Cuts at: 4482 4482
Size: 6287
SacI G_AGCT'C
Cuts at: 1354 3301 1354
Size: 1947 4340
SacII CC_GC'GG
Cuts at: 2063 2182 2063
Size: 119 6168
SalI G'TCGA_C
Cuts at: 3324 3324
Size: 6287
SapI GCTCTTCn'nnn_
Cuts at: 4310 4520 4310
Size: 210 6077
SbfI CC_TGCA'GG
Cuts at: 3334 3334
Size: 6287
ScaI AGT'ACT
Cuts at: 1678 5804 1678
Size: 4126 2161
SfcI C'TryA_G
Cuts at: 33 140 892 1594 1606 2052 2491 3149 3330
Size: 107 752 702 12 446 439 658 181
Cuts at: 3330 4015 5008 5199 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 752 702 685 658 446 439
191 181 107 12
SfoI GGC'GCC
Cuts at: 3719 3967 3719
Size: 248 6039
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
SmaI CCC'GGG
Cuts at: 3309 3309
Size: 6287
SmlI C'TyrA_G
Cuts at: 209 308 366 1552 1750 1961 2703 3442 4357
Size: 99 58 1186 198 211 742 739 915
Cuts at: 4357 4849 5111 209
Size: 492 262 1385
Fragments arranged by size:
1385 1186 915 742 739 492 262 211
198 99 58
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6287
SphI G_CATG'C
Cuts at: 3340 3840 3340
Size: 500 5787
SspI AAT'ATT
Cuts at: 1305 1305
Size: 6287
StuI AGG'CCT
Cuts at: 513 1296 2140 513
Size: 783 844 4660
Fragments arranged by size:
4660 844 783
StyI C'CwwG_G
Cuts at: 467 1379 1784 1925 2497 2813 3132 3421 3775
Size: 912 405 141 572 316 319 289 354
Cuts at: 3775 467
Size: 2979
Fragments arranged by size:
2979 912 572 405 354 319 316 289
141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1102 3870 4003 4462 4671 1102
Size: 2768 133 459 209 2718
Fragments arranged by size:
2768 2718 459 209 133
TatI w'GTAC_w
Cuts at: 485 521 1187 1676 2027 5802 485
Size: 36 666 489 351 3775 970
Fragments arranged by size:
3775 970 666 489 351 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 739 763 771 795 5804 5836 739
Size: 24 8 24 5009 32 1190
Fragments arranged by size:
5009 1190 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 125 637 3048 4084 125
Size: 512 2411 1036 2328
Fragments arranged by size:
2411 2328 1036 512
XbaI T'CTAG_A
Cuts at: 3318 3318
Size: 6287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3782 3782
Size: 6287
XhoI C'TCGA_G
Cuts at: 2703 2703
Size: 6287
XmaI C'CCGG_G
Cuts at: 3307 3307
Size: 6287
XmnI GAAnn'nnTTC
Cuts at: 2468 3064 2468
Size: 596 5691
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmBI BstEII BstZ17I
Bsu36I EcoNI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PsiI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI