Restriction map for
pF1KB4000
(Circular) (Six-base) MAPSORT of: pF1KB4000.seq Check: 358 from: 1 to: 3755
pF1KB4000 3755 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1580 2012 2759 3027 1580
Size: 432 747 268 2308
Fragments arranged by size:
2308 747 432 268
AflIII A'CryG_T
Cuts at: 1107 2211 3188 1107
Size: 1104 977 1674
Fragments arranged by size:
1674 1104 977
AgeI A'CCGG_T
Cuts at: 2143 2143
Size: 3755
AleI CACnn'nnGTG
Cuts at: 388 388
Size: 3755
AlwNI CAG_nnn'CTG
Cuts at: 137 289 737 1060 2627 137
Size: 152 448 323 1567 1265
Fragments arranged by size:
1567 1265 448 323 152
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 759 3108 3364 759
Size: 2349 256 1150
Fragments arranged by size:
2349 1150 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 457 775 457
Size: 318 3437
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 196 771 1185 1433 1468 196
Size: 575 414 248 35 2483
Fragments arranged by size:
2483 575 414 248 35
BanII G_rGCy'C
Cuts at: 464 769 1799 464
Size: 305 1030 2420
Fragments arranged by size:
2420 1030 305
BbeI G_GCGC'C
Cuts at: 1189 1437 1189
Size: 248 3507
BbvCI CC'TCA_GC
Cuts at: 361 361
Size: 3755
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 867 867
Size: 3755
Bme1580I G_kGCm'C
Cuts at: 263 1380 1473 2529 263
Size: 1117 93 1056 1489
Fragments arranged by size:
1489 1117 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 967 1048 1383 3527 967
Size: 81 335 2144 1195
Fragments arranged by size:
2144 1195 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
Bpu10I CC'TnA_GC
Cuts at: 361 361
Size: 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 931 1810 2302 2600 448
Size: 483 879 492 298 1603
Fragments arranged by size:
1603 879 492 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 658 3459 658
Size: 2801 954
BsaAI yAC'GTr
Cuts at: 258 483 1108 258
Size: 225 625 2905
Fragments arranged by size:
2905 625 225
BsaBI GATnn'nnATC
Cuts at: 6 1269 3306 6
Size: 1263 2037 455
Fragments arranged by size:
2037 1263 455
BsaHI Gr'CG_yC
Cuts at: 239 1186 1434 2136 3632 239
Size: 947 248 702 1496 362
Fragments arranged by size:
1496 947 702 362 248
BsaWI w'CCGG_w
Cuts at: 944 1465 2143 2417 2564 2975 944
Size: 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1180 1938 2515 3011 1180
Size: 758 577 496 1924
Fragments arranged by size:
1924 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 608 1297 1343 2551 104
Size: 504 689 46 1208 1308
Fragments arranged by size:
1308 1208 689 504 46
BsiHKAI G_wGCw'C
Cuts at: 464 615 769 1547 1737 2529 464
Size: 151 154 778 190 792 1690
Fragments arranged by size:
1690 792 778 190 154 151
Bsp1286I G_dGCh'C
Cuts at: 263 464 615 769 1380 1473 1547 1737 1799
Size: 201 151 154 611 93 74 190 62
Cuts at: 1799 2529 263
Size: 730 1489
Fragments arranged by size:
1489 730 611 201 190 154 151 93
74 62
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 805 1019 1321 1702 220
Size: 585 214 302 381 2273
Fragments arranged by size:
2273 585 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 1667
Size: 3755
BsrFI r'CCGG_y
Cuts at: 1753 1934 2143 1753
Size: 181 209 3365
Fragments arranged by size:
3365 209 181
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 315 1132 315
Size: 817 2938
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstYI r'GATC_y
Cuts at: 1 780 1247 1270 1605 1851 2863 2949 2961
Size: 779 467 23 335 246 1012 86 12
Cuts at: 2961 3307 1
Size: 346 449
Fragments arranged by size:
1012 779 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 200 335 200
Size: 135 3620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 998 998
Size: 3755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 252 287 252
Size: 35 3720
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2319 1461
Size: 858 2897
EaeI y'GGCC_r
Cuts at: 1340 1514 1905 1932 3277 1340
Size: 174 391 27 1345 1818
Fragments arranged by size:
1818 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1963 2273 2419 3565 1963
Size: 310 146 1146 2153
Fragments arranged by size:
2153 1146 310 146
EcoICRI GAG'CTC
Cuts at: 462 767 462
Size: 305 3450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1580 2012 2759 3027 1580
Size: 432 747 268 2308
Fragments arranged by size:
2308 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 453 503 894 453
Size: 50 391 3314
Fragments arranged by size:
3314 391 50
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 330 1774 330
Size: 1444 2311
FspI TGC'GCA
Cuts at: 1536 1536
Size: 3755
HaeII r_GCGC'y
Cuts at: 1189 1437 2459 3438 1189
Size: 248 1022 979 1506
Fragments arranged by size:
1506 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1597 1627 1629 1659 1597
Size: 30 2 30 3693
Fragments arranged by size:
3693 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 1185 1433 1185
Size: 248 3507
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 1516 1516
Size: 3755
MslI CAynn'nnrTG
Cuts at: 388 1744 1871 388
Size: 1356 127 2272
Fragments arranged by size:
2272 1356 127
MspA1I CmG'CkG
Cuts at: 137 337 360 734 866 1180 1540 2553 2798
Size: 200 23 374 132 314 360 1013 245
Cuts at: 2798 137
Size: 1094
Fragments arranged by size:
1094 1013 374 360 314 245 200 132
23
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 1186 1434 1186
Size: 248 3507
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NspI r_CATG'y
Cuts at: 477 808 1308 2215 477
Size: 331 500 907 2017
Fragments arranged by size:
2017 907 500 331
PasI CC'CwG_GG
Cuts at: 247 247
Size: 3755
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PfoI T'CCnGG_A
Cuts at: 440 440
Size: 3755
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PmlI CAC'GTG
Cuts at: 483 483
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PpuMI rG'GwC_Cy
Cuts at: 453 503 453
Size: 50 3705
PstI C_TGCA'G
Cuts at: 148 802 1487 148
Size: 654 685 2416
Fragments arranged by size:
2416 685 654
PvuI CG_AT'CG
Cuts at: 104 608 104
Size: 504 3251
PvuII CAG'CTG
Cuts at: 137 360 734 1180 1540 137
Size: 223 374 446 360 2352
Fragments arranged by size:
2352 446 374 360 223
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 464 769 464
Size: 305 3450
SacII CC_GC'GG
Cuts at: 338 338
Size: 3755
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SanDI GG'GwC_CC
Cuts at: 453 503 453
Size: 50 3705
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SfcI C'TryA_G
Cuts at: 33 144 798 1483 2476 2667 33
Size: 111 654 685 993 191 1121
Fragments arranged by size:
1121 993 685 654 191 111
SfoI GGC'GCC
Cuts at: 1187 1435 1187
Size: 248 3507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 777 777
Size: 3755
SmlI C'TyrA_G
Cuts at: 463 910 1825 2317 2579 463
Size: 447 915 492 262 1639
Fragments arranged by size:
1639 915 492 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 477 808 1308 477
Size: 331 500 2924
Fragments arranged by size:
2924 500 331
StyI C'CwwG_G
Cuts at: 536 889 1243 536
Size: 353 354 3048
Fragments arranged by size:
3048 354 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 3270 3270
Size: 3755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 1552 1552
Size: 3755
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1250 1250
Size: 3755
XmaI C'CCGG_G
Cuts at: 775 775
Size: 3755
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI