Restriction map for
pF1KB4009
(Circular) (Six-base) MAPSORT of: pF1KB4009.seq Check: 585 from: 1 to: 5504
pF1KB4009 5504 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 417 1607 417
Size: 1190 4314
AatII G_ACGT'C
Cuts at: 1439 1439
Size: 5504
AccI GT'mk_AC
Cuts at: 2171 2542 2171
Size: 371 5133
Acc65I G'GTAC_C
Cuts at: 2520 2520
Size: 5504
AclI AA'CG_TT
Cuts at: 5344 5344
Size: 5504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 454 1277 3329 3761 4114 4776 289
Size: 165 823 2052 432 353 662 1017
Fragments arranged by size:
2052 1017 823 662 432 353 165
AfeI AGC'GCT
Cuts at: 1530 1530
Size: 5504
AflII C'TTAA_G
Cuts at: 363 363
Size: 5504
AflIII A'CryG_T
Cuts at: 1310 2856 4656 4937 1310
Size: 1546 1800 281 1877
Fragments arranged by size:
1877 1800 1546 281
AgeI A'CCGG_T
Cuts at: 2412 2412
Size: 5504
AleI CACnn'nnGTG
Cuts at: 1420 1420
Size: 5504
AlwNI CAG_nnn'CTG
Cuts at: 1593 2809 4247 1593
Size: 1216 1438 2850
Fragments arranged by size:
2850 1438 1216
ApaI G_GGCC'C
Cuts at: 2299 2299
Size: 5504
ApaLI G'TGCA_C
Cuts at: 4342 4342
Size: 5504
ApoI r'AATT_y
Cuts at: 829 2395 2508 4857 5113 829
Size: 1566 113 2349 256 1220
Fragments arranged by size:
2349 1566 1220 256 113
AseI AT'TA_AT
Cuts at: 20 812 989 20
Size: 792 177 4535
Fragments arranged by size:
4535 792 177
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5504
AvaI C'yCGr_G
Cuts at: 299 682 1838 1893 1942 2524 299
Size: 383 1156 55 49 582 3279
Fragments arranged by size:
3279 1156 582 383 55 49
BamHI G'GATC_C
Cuts at: 2529 2529
Size: 5504
BanI G'GyrC_C
Cuts at: 1549 1702 1833 2136 2286 2329 2520 2934 3182
Size: 153 131 303 150 43 191 414 248
Cuts at: 3182 3217 1549
Size: 35 3836
Fragments arranged by size:
3836 414 303 248 191 153 150 131
43 35
BanII G_rGCy'C
Cuts at: 190 300 392 722 731 1943 2299 2518 3548
Size: 110 92 330 9 1212 356 219 1030
Cuts at: 3548 190
Size: 2146
Fragments arranged by size:
2146 1212 1030 356 330 219 110 92
9
BbeI G_GCGC'C
Cuts at: 1706 2938 3186 1706
Size: 1232 248 4024
Fragments arranged by size:
4024 1232 248
BbsI GAAGACnn'nnnn_
Cuts at: 1274 2192 1274
Size: 918 4586
BciVI GTATCCnnnnn_n'
Cuts at: 1225 1382 3402 4458 5504 1225
Size: 157 2020 1056 1046 1225
Fragments arranged by size:
2020 1225 1056 1046 157
BclI T'GATC_A
Cuts at: 489 3024 489
Size: 2535 2969
BglI GCCn_nnn'nGGC
Cuts at: 1681 1681
Size: 5504
BglII A'GATC_T
Cuts at: 3019 5056 3019
Size: 2037 3467
BlpI GC'TnA_GC
Cuts at: 1996 2616 1996
Size: 620 4884
Bme1580I G_kGCm'C
Cuts at: 1838 2299 3129 3222 4346 1838
Size: 461 830 93 1124 2996
Fragments arranged by size:
2996 1124 830 461 93
BmgBI CAC'GTC
Cuts at: 2322 2322
Size: 5504
BmrI ACTGGGnnnn_n'
Cuts at: 359 2716 2797 3132 5276 359
Size: 2357 81 335 2144 587
Fragments arranged by size:
2357 2144 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1068 3871 1068
Size: 2803 2701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 1022 219
Size: 803 4701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1912 2680 3559 4273 4571 1912
Size: 768 879 714 298 2845
Fragments arranged by size:
2845 879 768 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 312 1186 1320 1434 1623 5208 312
Size: 874 134 114 189 3585 608
Fragments arranged by size:
3585 874 608 189 134 114
BsaAI yAC'GTr
Cuts at: 1116 2857 1116
Size: 1741 3763
BsaBI GATnn'nnATC
Cuts at: 6 3018 5055 6
Size: 3012 2037 455
Fragments arranged by size:
3012 2037 455
BsaHI Gr'CG_yC
Cuts at: 1436 1703 1766 2935 3183 3885 5381 1436
Size: 267 63 1169 248 702 1496 1559
Fragments arranged by size:
1559 1496 1169 702 267 248 63
BsaWI w'CCGG_w
Cuts at: 1263 2412 2693 3214 3892 4303 4450 4724 1263
Size: 1149 281 521 678 411 147 274 2043
Fragments arranged by size:
2043 1149 678 521 411 281 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 506 536 1702 1732 2089 2119 506
Size: 30 1166 30 357 30 3891
Fragments arranged by size:
3891 1166 357 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 2268 400
Size: 1868 3636
BseYI C'CCAG_C
Cuts at: 2108 2929 3687 4352 4760 2108
Size: 821 758 665 408 2852
Fragments arranged by size:
2852 821 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 1579 1806 2268 1495
Size: 84 227 462 4731
Fragments arranged by size:
4731 462 227 84
BsiEI CG_ry'CG
Cuts at: 104 1847 3046 3092 4322 104
Size: 1743 1199 46 1230 1286
Fragments arranged by size:
1743 1286 1230 1199 46
BsiHKAI G_wGCw'C
Cuts at: 392 722 731 1023 1348 1412 2090 2225 2518
Size: 330 9 292 325 64 678 135 293
Cuts at: 2518 3296 3486 4346 392
Size: 778 190 860 1550
Fragments arranged by size:
1550 860 778 678 330 325 293 292
190 135 64 9
Bsp1286I G_dGCh'C
Cuts at: 190 300 392 722 731 1023 1348 1412 1838
Size: 110 92 330 9 292 325 64 426
Cuts at: 1838 1943 2090 2225 2299 2518 3129 3222 3296
Size: 105 147 135 74 219 611 93 74
Cuts at: 3296 3486 3548 4346 190
Size: 190 62 798 1348
Fragments arranged by size:
1348 798 611 426 330 325 292 219
190 147 135 110 105 93 92 74
74 64 62 9
BspEI T'CCGG_A
Cuts at: 1263 1263
Size: 5504
BspHI T'CATG_A
Cuts at: 3936 3936
Size: 5504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 417 1601 1607 2126 2554 2768 3070 3451 417
Size: 1184 6 519 428 214 302 381 2470
Fragments arranged by size:
2470 1184 519 428 381 302 214 6
BsrBI CCG'CTC
Cuts at: 50 230 2124 2327 3796 3850 5334 5499 50
Size: 180 1894 203 1469 54 1484 165 55
Fragments arranged by size:
1894 1484 1469 203 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3416 3416
Size: 5504
BsrFI r'CCGG_y
Cuts at: 2139 2412 3502 3683 2139
Size: 273 1090 181 3960
Fragments arranged by size:
3960 1090 273 181
BssSI C'ACGA_G
Cuts at: 3775 4483 3775
Size: 708 4796
BstAPI GCAn_nnn'nTGC
Cuts at: 150 2881 150
Size: 2731 2773
BstBI TT'CG_AA
Cuts at: 2475 3865 2475
Size: 1390 4114
BstEII G'GTnAC_C
Cuts at: 1863 1863
Size: 5504
BstXI CCAn_nnnn'nTGG
Cuts at: 1875 1875
Size: 5504
BstYI r'GATC_y
Cuts at: 1 2529 2996 3019 3354 3600 3906 3918 4004
Size: 2528 467 23 335 246 306 12 86
Cuts at: 4004 5056 1
Size: 1052 449
Fragments arranged by size:
2528 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1654 2129 2291 1654
Size: 475 162 4867
Fragments arranged by size:
4867 475 162
BtgI C'CryG_G
Cuts at: 107 1196 1713 1934 107
Size: 1089 517 221 3677
Fragments arranged by size:
3677 1089 517 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3454 3635 3798 3454
Size: 181 163 5160
Fragments arranged by size:
5160 181 163
BtsI GCAGTG_nn'
Cuts at: 1527 2747 1527
Size: 1220 4284
DraI TTT'AAA
Cuts at: 2503 2503
Size: 5504
DrdI GACnn_nn'nnGTC
Cuts at: 3210 4554 3210
Size: 1344 4160
EaeI y'GGCC_r
Cuts at: 1638 1844 2012 2066 3089 3263 3654 3681 5026
Size: 206 168 54 1023 174 391 27 1345
Cuts at: 5026 1638
Size: 2116
Fragments arranged by size:
2116 1345 1023 391 206 174 168 54
27
EagI C'GGCC_G
Cuts at: 1844 3089 1844
Size: 1245 4259
EarI CTCTTCn'nnn_
Cuts at: 710 2212 2218 3527 3737 710
Size: 1502 6 1309 210 2477
Fragments arranged by size:
2477 1502 1309 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3712 4454 4600 5314 3712
Size: 742 146 714 3902
Fragments arranged by size:
3902 742 714 146
EcoICRI GAG'CTC
Cuts at: 390 720 729 2516 390
Size: 330 9 1787 3378
Fragments arranged by size:
3378 1787 330 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 289 454 1022 1277 3329 3761 4114 4776
Size: 70 165 568 255 2052 432 353 662
Cuts at: 4776 219
Size: 947
Fragments arranged by size:
2052 947 662 568 432 353 255 165
70
EcoNI CCTnn'n_nnAGG
Cuts at: 789 789
Size: 5504
EcoO109I rG'GnC_Cy
Cuts at: 258 761 1590 1674 2295 2296 2336 2643 258
Size: 503 829 84 621 1 40 307 3119
Fragments arranged by size:
3119 829 621 503 307 84 40 1
EcoRI G'AATT_C
Cuts at: 2395 2508 2395
Size: 113 5391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 169 2316 3523 169
Size: 2147 1207 2150
Fragments arranged by size:
2150 2147 1207
FspI TGC'GCA
Cuts at: 1981 3285 1981
Size: 1304 4200
FspAI rTGC'GCAy
Cuts at: 1981 1981
Size: 5504
HaeII r_GCGC'y
Cuts at: 706 1532 1706 1744 2938 3186 4416 5187 706
Size: 826 174 38 1194 248 1230 771 1023
Fragments arranged by size:
1230 1194 1023 826 771 248 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 745 777 901 933 1213 1245 1528
Size: 32 599 32 124 32 280 32 283
Cuts at: 1528 1560 1702 1734 1876 1908 2089 2121 3346
Size: 32 142 32 142 32 181 32 1225
Cuts at: 3346 3376 3378 3408 114
Size: 30 2 30 2210
Fragments arranged by size:
2210 1225 599 283 280 181 142 142
124 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1566 2543 1566
Size: 977 4527
KasI G'GCGC_C
Cuts at: 1702 2934 3182 1702
Size: 1232 248 4024
Fragments arranged by size:
4024 1232 248
KpnI G_GTAC'C
Cuts at: 2524 2524
Size: 5504
MluI A'CGCG_T
Cuts at: 4937 4937
Size: 5504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1295 4263 4447 1295
Size: 2968 184 2352
Fragments arranged by size:
2968 2352 184
MscI TGG'CCA
Cuts at: 3265 3265
Size: 5504
MslI CAynn'nnrTG
Cuts at: 649 1420 3493 3620 649
Size: 771 2073 127 2533
Fragments arranged by size:
2533 2073 771 127
MspA1I CmG'CkG
Cuts at: 336 1715 1800 1923 2615 2929 3289 4073 4318
Size: 1379 85 123 692 314 360 784 245
Cuts at: 4318 336
Size: 1522
Fragments arranged by size:
1522 1379 784 692 360 314 245 123
85
NaeI GCC'GGC
Cuts at: 3685 3685
Size: 5504
NarI GG'CG_CC
Cuts at: 1703 2935 3183 1703
Size: 1232 248 4024
Fragments arranged by size:
4024 1232 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5504
NgoMIV G'CCGG_C
Cuts at: 3683 3683
Size: 5504
NspI r_CATG'y
Cuts at: 2557 3057 4660 2557
Size: 500 1603 3401
Fragments arranged by size:
3401 1603 500
PciI A'CATG_T
Cuts at: 4656 4656
Size: 5504
PflMI CCAn_nnn'nTGG
Cuts at: 1314 1314
Size: 5504
PfoI T'CCnGG_A
Cuts at: 722 722
Size: 5504
PmeI GTTT'AAAC
Cuts at: 2503 2503
Size: 5504
PmlI CAC'GTG
Cuts at: 1116 1116
Size: 5504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 3950
Size: 32 5472
PpuMI rG'GwC_Cy
Cuts at: 258 761 1590 2336 258
Size: 503 829 746 3426
Fragments arranged by size:
3426 829 746 503
PspOMI G'GGCC_C
Cuts at: 2295 2295
Size: 5504
PstI C_TGCA'G
Cuts at: 1682 1805 2551 3236 1682
Size: 123 746 685 3950
Fragments arranged by size:
3950 746 685 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5504
PvuII CAG'CTG
Cuts at: 336 1923 2929 3289 336
Size: 1587 1006 360 2551
Fragments arranged by size:
2551 1587 1006 360
RsrII CG'GwC_CG
Cuts at: 3699 3699
Size: 5504
SacI G_AGCT'C
Cuts at: 392 722 731 2518 392
Size: 330 9 1787 3378
Fragments arranged by size:
3378 1787 330 9
SacII CC_GC'GG
Cuts at: 1716 1716
Size: 5504
SalI G'TCGA_C
Cuts at: 2541 2541
Size: 5504
SanDI GG'GwC_CC
Cuts at: 761 2336 761
Size: 1575 3929
SapI GCTCTTCn'nnn_
Cuts at: 710 2212 3527 3737 710
Size: 1502 1315 210 2477
Fragments arranged by size:
2477 1502 1315 210
SbfI CC_TGCA'GG
Cuts at: 1682 2551 1682
Size: 869 4635
ScaI AGT'ACT
Cuts at: 5021 5021
Size: 5504
SexAI A'CCwGG_T
Cuts at: 1859 1859
Size: 5504
SfcI C'TryA_G
Cuts at: 33 1100 1532 1678 1801 2547 3232 4200 4391
Size: 1067 432 146 123 746 685 968 191
Cuts at: 4391 33
Size: 1146
Fragments arranged by size:
1146 1067 968 746 685 432 191 146
123
SfoI GGC'GCC
Cuts at: 1704 2936 3184 1704
Size: 1232 248 4024
Fragments arranged by size:
4024 1232 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5504
SmaI CCC'GGG
Cuts at: 1840 1944 2526 1840
Size: 104 582 4818
Fragments arranged by size:
4818 582 104
SmlI C'TyrA_G
Cuts at: 363 1927 2659 3574 4288 4550 363
Size: 1564 732 915 714 262 1317
Fragments arranged by size:
1564 1317 915 732 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5504
SphI G_CATG'C
Cuts at: 2557 3057 2557
Size: 500 5004
SrfI GCCC'GGGC
Cuts at: 1944 1944
Size: 5504
StyI C'CwwG_G
Cuts at: 107 117 785 876 1868 2638 2992 107
Size: 10 668 91 992 770 354 2619
Fragments arranged by size:
2619 992 770 668 354 91 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1477 3087 3220 3679 3888 1477
Size: 1610 133 459 209 3093
Fragments arranged by size:
3093 1610 459 209 133
TatI w'GTAC_w
Cuts at: 1355 5019 1355
Size: 3664 1840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 5021 5053 2439
Size: 32 2550 32 2890
Fragments arranged by size:
2890 2550 32 32
Tth111I GACn'n_nGTC
Cuts at: 3301 3301
Size: 5504
XbaI T'CTAG_A
Cuts at: 2535 2535
Size: 5504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 2999 2022
Size: 977 4527
XmaI C'CCGG_G
Cuts at: 1838 1942 2524 1838
Size: 104 582 4818
Fragments arranged by size:
4818 582 104
XmnI GAAnn'nnTTC
Cuts at: 968 968
Size: 5504
ZraI GAC'GTC
Cuts at: 1437 1437
Size: 5504
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AhdI AlfI AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI