Restriction map for
pF1KB4018
(Circular) (Six-base) MAPSORT of: pF1KB4018.seq Check: 8767 from: 1 to: 5795
pF1KB4018 5795 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 197 197
Size: 5795
AccI GT'mk_AC
Cuts at: 197 2833 197
Size: 2636 3159
Acc65I G'GTAC_C
Cuts at: 2811 2811
Size: 5795
AclI AA'CG_TT
Cuts at: 5635 5635
Size: 5795
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 2167 3620 4052 4405 5067 721
Size: 1446 1453 432 353 662 1449
Fragments arranged by size:
1453 1449 1446 662 432 353
AflII C'TTAA_G
Cuts at: 1131 1131
Size: 5795
AflIII A'CryG_T
Cuts at: 1329 3147 4947 5228 1329
Size: 1818 1800 281 1896
Fragments arranged by size:
1896 1818 1800 281
AleI CACnn'nnGTG
Cuts at: 1259 1259
Size: 5795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1757 1757
Size: 5795
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1889 1921 1889
Size: 32 5763
AlwNI CAG_nnn'CTG
Cuts at: 3100 4538 3100
Size: 1438 4357
ApaLI G'TGCA_C
Cuts at: 4633 4633
Size: 5795
ApoI r'AATT_y
Cuts at: 1049 1117 1397 1781 2083 2227 2385 2660 2678
Size: 68 280 384 302 144 158 275 18
Cuts at: 2678 2799 5148 5404 1049
Size: 121 2349 256 1440
Fragments arranged by size:
2349 1440 384 302 280 275 256 158
144 121 68 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5795
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5795
AvaI C'yCGr_G
Cuts at: 2815 2815
Size: 5795
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2293 2326 2293
Size: 33 5762
BamHI G'GATC_C
Cuts at: 2820 2820
Size: 5795
BanI G'GyrC_C
Cuts at: 181 1840 2120 2811 3225 3473 3508 181
Size: 1659 280 691 414 248 35 2468
Fragments arranged by size:
2468 1659 691 414 280 248 35
BanII G_rGCy'C
Cuts at: 1510 1597 2809 3839 1510
Size: 87 1212 1030 3466
Fragments arranged by size:
3466 1212 1030 87
BbeI G_GCGC'C
Cuts at: 3229 3477 3229
Size: 248 5547
BbsI GAAGACnn'nnnn_
Cuts at: 272 1282 1705 272
Size: 1010 423 4362
Fragments arranged by size:
4362 1010 423
BciVI GTATCCnnnnn_n'
Cuts at: 3693 4749 5795 3693
Size: 1056 1046 3693
Fragments arranged by size:
3693 1056 1046
BclI T'GATC_A
Cuts at: 3315 3315
Size: 5795
BfrBI ATG'CAT
Cuts at: 1452 1452
Size: 5795
BglII A'GATC_T
Cuts at: 1216 2638 3310 5347 1216
Size: 1422 672 2037 1664
Fragments arranged by size:
2037 1664 1422 672
BlpI GC'TnA_GC
Cuts at: 2907 2907
Size: 5795
Bme1580I G_kGCm'C
Cuts at: 3420 3513 4637 3420
Size: 93 1124 4578
Fragments arranged by size:
4578 1124 93
BmgBI CAC'GTC
Cuts at: 1332 1332
Size: 5795
BmrI ACTGGGnnnn_n'
Cuts at: 3007 3088 3423 5567 3007
Size: 81 335 2144 3235
Fragments arranged by size:
3235 2144 335 81
BmtI G_CTAG'C
Cuts at: 2602 2602
Size: 5795
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4162 4162
Size: 5795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 2473 629
Size: 1844 3951
Bpu10I CC'TnA_GC
Cuts at: 1022 1022
Size: 5795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2971 3850 4564 4862 1279
Size: 1692 879 714 298 2212
Fragments arranged by size:
2212 1692 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5499 5499
Size: 5795
BsaAI yAC'GTr
Cuts at: 417 3148 417
Size: 2731 3064
BsaBI GATnn'nnATC
Cuts at: 6 410 1016 1469 2520 3309 5346 6
Size: 404 606 453 1051 789 2037 455
Fragments arranged by size:
2037 1051 789 606 455 453 404
BsaHI Gr'CG_yC
Cuts at: 194 3226 3474 4176 5672 194
Size: 3032 248 702 1496 317
Fragments arranged by size:
3032 1496 702 317 248
BsaWI w'CCGG_w
Cuts at: 2984 3505 4183 4594 4741 5015 2984
Size: 521 678 411 147 274 3764
Fragments arranged by size:
3764 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2042 2072 2042
Size: 30 5765
BseYI C'CCAG_C
Cuts at: 1509 3220 3978 4643 5051 1509
Size: 1711 758 665 408 2253
Fragments arranged by size:
2253 1711 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 566 301
Size: 265 5530
BsiEI CG_ry'CG
Cuts at: 104 3337 3383 4613 104
Size: 3233 46 1230 1286
Fragments arranged by size:
3233 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1597 2181 2809 3587 3777 4637 1597
Size: 584 628 778 190 860 2755
Fragments arranged by size:
2755 860 778 628 584 190
BsmI GAATG_Cn'
Cuts at: 1452 2624 1452
Size: 1172 4623
BsmBI CGTCTCn'nnnn_
Cuts at: 2365 2365
Size: 5795
Bsp1286I G_dGCh'C
Cuts at: 1510 1597 2181 2809 3420 3513 3587 3777 3839
Size: 87 584 628 611 93 74 190 62
Cuts at: 3839 4637 1510
Size: 798 2668
Fragments arranged by size:
2668 798 628 611 584 190 93 87
74 62
BspHI T'CATG_A
Cuts at: 296 1155 1881 2775 4227 296
Size: 859 726 894 1452 1864
Fragments arranged by size:
1864 1452 894 859 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2845 3059 3361 3742 2845
Size: 214 302 381 4898
Fragments arranged by size:
4898 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4087 4141 5625 5790 50
Size: 4037 54 1484 165 55
Fragments arranged by size:
4037 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3707 3707
Size: 5795
BsrFI r'CCGG_y
Cuts at: 3793 3974 3793
Size: 181 5614
BsrGI T'GTAC_A
Cuts at: 1918 2759 1918
Size: 841 4954
BssSI C'ACGA_G
Cuts at: 4066 4774 4066
Size: 708 5087
BstAPI GCAn_nnn'nTGC
Cuts at: 3172 3172
Size: 5795
BstBI TT'CG_AA
Cuts at: 4156 4156
Size: 5795
BstXI CCAn_nnnn'nTGG
Cuts at: 1609 1609
Size: 5795
BstYI r'GATC_y
Cuts at: 1 1216 1227 2638 2695 2820 3287 3310 3645
Size: 1215 11 1411 57 125 467 23 335
Cuts at: 3645 3891 4197 4209 4295 5347 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1411 1215 1052 467 449 335 306 246
125 86 57 23 12 11
Bsu36I CC'TnA_GG
Cuts at: 1578 1578
Size: 5795
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3745 3926 4089 3745
Size: 181 163 5451
Fragments arranged by size:
5451 181 163
BtsI GCAGTG_nn'
Cuts at: 466 710 713 1208 3038 466
Size: 244 3 495 1830 3223
Fragments arranged by size:
3223 1830 495 244 3
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1391 1426 1391
Size: 35 5760
DraI TTT'AAA
Cuts at: 2794 2794
Size: 5795
DraIII CAC_nnn'GTG
Cuts at: 855 855
Size: 5795
DrdI GACnn_nn'nnGTC
Cuts at: 3501 4845 3501
Size: 1344 4451
EaeI y'GGCC_r
Cuts at: 1107 3380 3554 3945 3972 5317 1107
Size: 2273 174 391 27 1345 1585
Fragments arranged by size:
2273 1585 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3380 3380
Size: 5795
EarI CTCTTCn'nnn_
Cuts at: 2400 3818 4028 2400
Size: 1418 210 4167
Fragments arranged by size:
4167 1418 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4003 4745 4891 5605 4003
Size: 742 146 714 4193
Fragments arranged by size:
4193 742 714 146
EcoICRI GAG'CTC
Cuts at: 1595 2807 1595
Size: 1212 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 721 2167 2473 3620 4052 4405 5067 629
Size: 92 1446 306 1147 432 353 662 1357
Fragments arranged by size:
1446 1357 1147 662 432 353 306 92
EcoO109I rG'GnC_Cy
Cuts at: 2934 2934
Size: 5795
EcoRI G'AATT_C
Cuts at: 1049 2799 1049
Size: 1750 4045
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1449 2165 3814 1449
Size: 716 1649 3430
Fragments arranged by size:
3430 1649 716
FspI TGC'GCA
Cuts at: 3576 3576
Size: 5795
HaeII r_GCGC'y
Cuts at: 3229 3477 4707 5478 3229
Size: 248 1230 771 3546
Fragments arranged by size:
3546 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1316 1348 1354 1386 2364 2396 2450 2482 3637
Size: 32 6 32 978 32 54 32 1155
Cuts at: 3637 3667 3669 3699 1316
Size: 30 2 30 3412
Fragments arranged by size:
3412 1155 978 54 32 32 32 32
30 30 6 2
HincII GTy'rAC
Cuts at: 216 1636 2834 216
Size: 1420 1198 3177
Fragments arranged by size:
3177 1420 1198
HindIII A'AGCT_T
Cuts at: 361 1967 361
Size: 1606 4189
HpaI GTT'AAC
Cuts at: 216 216
Size: 5795
KasI G'GCGC_C
Cuts at: 3225 3473 3225
Size: 248 5547
KpnI G_GTAC'C
Cuts at: 2815 2815
Size: 5795
MfeI C'AATT_G
Cuts at: 599 1941 599
Size: 1342 4453
MluI A'CGCG_T
Cuts at: 5228 5228
Size: 5795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 944 1744 4554 4738 944
Size: 800 2810 184 2001
Fragments arranged by size:
2810 2001 800 184
MscI TGG'CCA
Cuts at: 1109 3556 1109
Size: 2447 3348
MslI CAynn'nnrTG
Cuts at: 1259 1739 1797 3784 3911 1259
Size: 480 58 1987 127 3143
Fragments arranged by size:
3143 1987 480 127 58
MspA1I CmG'CkG
Cuts at: 179 658 2906 3220 3580 4364 4609 179
Size: 479 2248 314 360 784 245 1365
Fragments arranged by size:
2248 1365 784 479 360 314 245
NaeI GCC'GGC
Cuts at: 3976 3976
Size: 5795
NarI GG'CG_CC
Cuts at: 3226 3474 3226
Size: 248 5547
NdeI CA'TA_TG
Cuts at: 450 1736 450
Size: 1286 4509
NgoMIV G'CCGG_C
Cuts at: 3974 3974
Size: 5795
NheI G'CTAG_C
Cuts at: 2598 2598
Size: 5795
NsiI A_TGCA'T
Cuts at: 1454 1454
Size: 5795
NspI r_CATG'y
Cuts at: 1748 2848 3348 4951 1748
Size: 1100 500 1603 2592
Fragments arranged by size:
2592 1603 1100 500
PacI TTA_AT'TAA
Cuts at: 2240 2240
Size: 5795
PciI A'CATG_T
Cuts at: 4947 4947
Size: 5795
PmeI GTTT'AAAC
Cuts at: 2794 2794
Size: 5795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 4241
Size: 32 5763
PshAI GACnn'nnGTC
Cuts at: 429 429
Size: 5795
PstI C_TGCA'G
Cuts at: 473 2842 3527 473
Size: 2369 685 2741
Fragments arranged by size:
2741 2369 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5795
PvuII CAG'CTG
Cuts at: 658 3220 3580 658
Size: 2562 360 2873
Fragments arranged by size:
2873 2562 360
RsrII CG'GwC_CG
Cuts at: 3990 3990
Size: 5795
SacI G_AGCT'C
Cuts at: 1597 2809 1597
Size: 1212 4583
SalI G'TCGA_C
Cuts at: 2832 2832
Size: 5795
SapI GCTCTTCn'nnn_
Cuts at: 3818 4028 3818
Size: 210 5585
SbfI CC_TGCA'GG
Cuts at: 2842 2842
Size: 5795
ScaI AGT'ACT
Cuts at: 1828 5312 1828
Size: 3484 2311
SfcI C'TryA_G
Cuts at: 33 469 2838 3523 4491 4682 33
Size: 436 2369 685 968 191 1146
Fragments arranged by size:
2369 1146 968 685 436 191
SfoI GGC'GCC
Cuts at: 3227 3475 3227
Size: 248 5547
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5795
SmaI CCC'GGG
Cuts at: 2817 2817
Size: 5795
SmlI C'TyrA_G
Cuts at: 1131 1294 2950 3865 4579 4841 1131
Size: 163 1656 915 714 262 2085
Fragments arranged by size:
2085 1656 915 714 262 163
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5795
SphI G_CATG'C
Cuts at: 2848 3348 2848
Size: 500 5295
SspI AAT'ATT
Cuts at: 324 324
Size: 5795
StyI C'CwwG_G
Cuts at: 1257 2077 2508 2929 3283 1257
Size: 820 431 421 354 3769
Fragments arranged by size:
3769 820 431 421 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 332 776 1729 1757 3378 3511 3970 4179 332
Size: 444 953 28 1621 133 459 209 1948
Fragments arranged by size:
1948 1621 953 459 444 209 133 28
TatI w'GTAC_w
Cuts at: 1826 1918 2759 5310 1826
Size: 92 841 2551 2311
Fragments arranged by size:
2551 2311 841 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 5312 5344 2042
Size: 32 3238 32 2493
Fragments arranged by size:
3238 2493 32 32
Tth111I GACn'n_nGTC
Cuts at: 942 2704 3592 942
Size: 1762 888 3145
Fragments arranged by size:
3145 1762 888
XbaI T'CTAG_A
Cuts at: 2826 2826
Size: 5795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3290 3290
Size: 5795
XmaI C'CCGG_G
Cuts at: 2815 2815
Size: 5795
XmnI GAAnn'nnTTC
Cuts at: 616 616
Size: 5795
ZraI GAC'GTC
Cuts at: 195 195
Size: 5795
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NsiI NspI
PacI PciI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI ApaI AscI AvrII BbvCI
BcgI BglI BseRI BsiWI BspEI BssHII BstEII BstZ17I
BtgI ClaI EcoNI EcoRV FseI FspAI NcoI NotI
NruI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI