Restriction map for
pF1KB4019
(Circular) (Six-base) MAPSORT of: pF1KB4019.seq Check: 1714 from: 1 to: 4403
pF1KB4019 4403 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1441 1441
Size: 4403
Acc65I G'GTAC_C
Cuts at: 1419 1419
Size: 4403
AclI AA'CG_TT
Cuts at: 1397 4243 1397
Size: 2846 1557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2660 3013 3675 2228
Size: 432 353 662 2956
Fragments arranged by size:
2956 662 432 353
AflIII A'CryG_T
Cuts at: 1124 1755 3555 3836 1124
Size: 631 1800 281 1691
Fragments arranged by size:
1800 1691 631 281
AleI CACnn'nnGTG
Cuts at: 640 640
Size: 4403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 155 155
Size: 4403
AlwNI CAG_nnn'CTG
Cuts at: 1708 3146 1708
Size: 1438 2965
ApaI G_GGCC'C
Cuts at: 1191 1191
Size: 4403
ApaLI G'TGCA_C
Cuts at: 3241 3241
Size: 4403
ApoI r'AATT_y
Cuts at: 822 997 1407 3756 4012 822
Size: 175 410 2349 256 1213
Fragments arranged by size:
2349 1213 410 256 175
AseI AT'TA_AT
Cuts at: 20 20
Size: 4403
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
AvaI C'yCGr_G
Cuts at: 1423 1423
Size: 4403
BamHI G'GATC_C
Cuts at: 1428 1428
Size: 4403
BanI G'GyrC_C
Cuts at: 1419 1833 2081 2116 1419
Size: 414 248 35 3706
Fragments arranged by size:
3706 414 248 35
BanII G_rGCy'C
Cuts at: 393 1191 1417 2447 393
Size: 798 226 1030 2349
Fragments arranged by size:
2349 1030 798 226
BbeI G_GCGC'C
Cuts at: 1837 2085 1837
Size: 248 4155
BciVI GTATCCnnnnn_n'
Cuts at: 391 1215 1249 2301 3357 4403 391
Size: 824 34 1052 1056 1046 391
Fragments arranged by size:
1056 1052 1046 824 391 34
BclI T'GATC_A
Cuts at: 1923 1923
Size: 4403
BfrBI ATG'CAT
Cuts at: 132 132
Size: 4403
BglII A'GATC_T
Cuts at: 1918 3955 1918
Size: 2037 2366
BlpI GC'TnA_GC
Cuts at: 1515 1515
Size: 4403
Bme1580I G_kGCm'C
Cuts at: 1191 2028 2121 3245 1191
Size: 837 93 1124 2349
Fragments arranged by size:
2349 1124 837 93
BmrI ACTGGGnnnn_n'
Cuts at: 884 1615 1696 2031 4175 884
Size: 731 81 335 2144 1112
Fragments arranged by size:
2144 1112 731 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 583 2770 583
Size: 2187 2216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 622 1157 536
Size: 86 535 3782
Fragments arranged by size:
3782 535 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1579 2458 3172 3470 736
Size: 843 879 714 298 1669
Fragments arranged by size:
1669 879 843 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4107 4107
Size: 4403
BsaAI yAC'GTr
Cuts at: 642 764 1756 642
Size: 122 992 3289
Fragments arranged by size:
3289 992 122
BsaBI GATnn'nnATC
Cuts at: 6 1917 3954 6
Size: 1911 2037 455
Fragments arranged by size:
2037 1911 455
BsaHI Gr'CG_yC
Cuts at: 1834 2082 2784 4280 1834
Size: 248 702 1496 1957
Fragments arranged by size:
1957 1496 702 248
BsaWI w'CCGG_w
Cuts at: 974 1592 2113 2791 3202 3349 3623 974
Size: 618 521 678 411 147 274 1754
Fragments arranged by size:
1754 678 618 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1190 1220 1190
Size: 30 4373
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 401 412 922 401
Size: 11 510 3882
Fragments arranged by size:
3882 510 11
BseYI C'CCAG_C
Cuts at: 142 176 416 1828 2586 3251 3659 142
Size: 34 240 1412 758 665 408 886
Fragments arranged by size:
1412 886 758 665 408 240 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 1177
Size: 4403
BsiEI CG_ry'CG
Cuts at: 104 1945 1991 3221 104
Size: 1841 46 1230 1286
Fragments arranged by size:
1841 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 763 1417 2195 2385 3245 763
Size: 654 778 190 860 1921
Fragments arranged by size:
1921 860 778 654 190
BsmI GAATG_Cn'
Cuts at: 132 546 132
Size: 414 3989
BsmBI CGTCTCn'nnnn_
Cuts at: 810 810
Size: 4403
Bsp1286I G_dGCh'C
Cuts at: 393 763 1191 1417 2028 2121 2195 2385 2447
Size: 370 428 226 611 93 74 190 62
Cuts at: 2447 3245 393
Size: 798 1551
Fragments arranged by size:
1551 798 611 428 370 226 190 93
74 62
BspHI T'CATG_A
Cuts at: 1148 2835 1148
Size: 1687 2716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 514 1453 1667 1969 2350 514
Size: 939 214 302 381 2567
Fragments arranged by size:
2567 939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 702 2695 2749 4233 4398 50
Size: 652 1993 54 1484 165 55
Fragments arranged by size:
1993 1484 652 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2315 2315
Size: 4403
BsrFI r'CCGG_y
Cuts at: 2401 2582 2401
Size: 181 4222
BsrGI T'GTAC_A
Cuts at: 1286 1286
Size: 4403
BssSI C'ACGA_G
Cuts at: 2674 3382 2674
Size: 708 3695
BstAPI GCAn_nnn'nTGC
Cuts at: 1780 1780
Size: 4403
BstBI TT'CG_AA
Cuts at: 2764 2764
Size: 4403
BstYI r'GATC_y
Cuts at: 1 1428 1895 1918 2253 2499 2805 2817 2903
Size: 1427 467 23 335 246 306 12 86
Cuts at: 2903 3955 1
Size: 1052 449
Fragments arranged by size:
1427 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1062 107
Size: 955 3448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2353 2534 2697 2353
Size: 181 163 4059
Fragments arranged by size:
4059 181 163
BtsI GCAGTG_nn'
Cuts at: 1040 1380 1646 1040
Size: 340 266 3797
Fragments arranged by size:
3797 340 266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 906 941 906
Size: 35 4368
DraI TTT'AAA
Cuts at: 311 1402 311
Size: 1091 3312
DrdI GACnn_nn'nnGTC
Cuts at: 2109 3453 2109
Size: 1344 3059
EaeI y'GGCC_r
Cuts at: 155 1988 2162 2553 2580 3925 155
Size: 1833 174 391 27 1345 633
Fragments arranged by size:
1833 1345 633 391 174 27
EagI C'GGCC_G
Cuts at: 1988 1988
Size: 4403
EarI CTCTTCn'nnn_
Cuts at: 808 2426 2636 808
Size: 1618 210 2575
Fragments arranged by size:
2575 1618 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 491 825 2611 3353 3499 4213 491
Size: 334 1786 742 146 714 681
Fragments arranged by size:
1786 742 714 681 334 146
EcoICRI GAG'CTC
Cuts at: 1415 1415
Size: 4403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 622 1157 2228 2660 3013 3675 536
Size: 86 535 1071 432 353 662 1264
Fragments arranged by size:
1264 1071 662 535 432 353 86
EcoNI CCTnn'n_nnAGG
Cuts at: 1361 1361
Size: 4403
EcoO109I rG'GnC_Cy
Cuts at: 463 1187 1366 1542 463
Size: 724 179 176 3324
Fragments arranged by size:
3324 724 179 176
EcoRI G'AATT_C
Cuts at: 1407 1407
Size: 4403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 351 2422 351
Size: 2071 2332
FspI TGC'GCA
Cuts at: 2184 2184
Size: 4403
HaeII r_GCGC'y
Cuts at: 1837 2085 3315 4086 1837
Size: 248 1230 771 2154
Fragments arranged by size:
2154 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1275 1307 2245 2275 2277 2307 1275
Size: 32 938 30 2 30 3371
Fragments arranged by size:
3371 938 32 30 30 2
HincII GTy'rAC
Cuts at: 1442 1442
Size: 4403
KasI G'GCGC_C
Cuts at: 1833 2081 1833
Size: 248 4155
KpnI G_GTAC'C
Cuts at: 1423 1423
Size: 4403
MluI A'CGCG_T
Cuts at: 3836 3836
Size: 4403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1176 1418 3162 3346 1176
Size: 242 1744 184 2233
Fragments arranged by size:
2233 1744 242 184
MscI TGG'CCA
Cuts at: 2164 2164
Size: 4403
MslI CAynn'nnrTG
Cuts at: 137 640 742 1153 2392 2519 137
Size: 503 102 411 1239 127 2021
Fragments arranged by size:
2021 1239 503 411 127 102
MspA1I CmG'CkG
Cuts at: 420 493 502 1036 1514 1828 2188 2972 3217
Size: 73 9 534 478 314 360 784 245
Cuts at: 3217 420
Size: 1606
Fragments arranged by size:
1606 784 534 478 360 314 245 73
9
NaeI GCC'GGC
Cuts at: 2584 2584
Size: 4403
NarI GG'CG_CC
Cuts at: 1834 2082 1834
Size: 248 4155
NcoI C'CATG_G
Cuts at: 107 1062 107
Size: 955 3448
NgoMIV G'CCGG_C
Cuts at: 2582 2582
Size: 4403
NsiI A_TGCA'T
Cuts at: 134 134
Size: 4403
NspI r_CATG'y
Cuts at: 307 441 1128 1456 1956 3559 307
Size: 134 687 328 500 1603 1151
Fragments arranged by size:
1603 1151 687 500 328 134
PciI A'CATG_T
Cuts at: 1124 3555 1124
Size: 2431 1972
PflMI CCAn_nnn'nTGG
Cuts at: 279 279
Size: 4403
PmeI GTTT'AAAC
Cuts at: 1402 1402
Size: 4403
PmlI CAC'GTG
Cuts at: 642 764 642
Size: 122 4281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 4371
PspOMI G'GGCC_C
Cuts at: 1187 1187
Size: 4403
PstI C_TGCA'G
Cuts at: 1450 2135 1450
Size: 685 3718
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4403
PvuII CAG'CTG
Cuts at: 420 1036 1828 2188 420
Size: 616 792 360 2635
Fragments arranged by size:
2635 792 616 360
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 4403
SacI G_AGCT'C
Cuts at: 1417 1417
Size: 4403
SalI G'TCGA_C
Cuts at: 1440 1440
Size: 4403
SapI GCTCTTCn'nnn_
Cuts at: 2426 2636 2426
Size: 210 4193
SbfI CC_TGCA'GG
Cuts at: 1450 1450
Size: 4403
ScaI AGT'ACT
Cuts at: 3920 3920
Size: 4403
SfcI C'TryA_G
Cuts at: 33 1358 1446 2131 3099 3290 33
Size: 1325 88 685 968 191 1146
Fragments arranged by size:
1325 1146 968 685 191 88
SfoI GGC'GCC
Cuts at: 1835 2083 1835
Size: 248 4155
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
SmaI CCC'GGG
Cuts at: 1425 1425
Size: 4403
SmlI C'TyrA_G
Cuts at: 751 1558 2473 3187 3449 751
Size: 807 915 714 262 1705
Fragments arranged by size:
1705 915 807 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4403
SphI G_CATG'C
Cuts at: 1456 1956 1456
Size: 500 3903
SspI AAT'ATT
Cuts at: 859 1017 859
Size: 158 4245
StuI AGG'CCT
Cuts at: 619 619
Size: 4403
StyI C'CwwG_G
Cuts at: 107 1062 1182 1537 1891 107
Size: 955 120 355 354 2619
Fragments arranged by size:
2619 955 355 354 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 652 1986 2119 2578 2787 220
Size: 432 1334 133 459 209 1836
Fragments arranged by size:
1836 1334 459 432 209 133
TatI w'GTAC_w
Cuts at: 1286 3918 1286
Size: 2632 1771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1190 1222 3920 3952 1190
Size: 32 2698 32 1641
Fragments arranged by size:
2698 1641 32 32
Tth111I GACn'n_nGTC
Cuts at: 2200 2200
Size: 4403
XbaI T'CTAG_A
Cuts at: 1434 1434
Size: 4403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1898 1898
Size: 4403
XmaI C'CCGG_G
Cuts at: 1423 1423
Size: 4403
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsiWI BspEI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI