Restriction map for
pF1KB4020
(Circular) (Six-base) MAPSORT of: pF1KB4020.seq Check: 8423 from: 1 to: 5105
pF1KB4020 5105 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1575 1575
Size: 5105
AccI GT'mk_AC
Cuts at: 239 2143 239
Size: 1904 3201
Acc65I G'GTAC_C
Cuts at: 2121 2121
Size: 5105
AclI AA'CG_TT
Cuts at: 4945 4945
Size: 5105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 529 640 1594 2930 3362 3715 4377 326
Size: 203 111 954 1336 432 353 662 1054
Fragments arranged by size:
1336 1054 954 662 432 353 203 111
AflIII A'CryG_T
Cuts at: 2457 4257 4538 2457
Size: 1800 281 3024
Fragments arranged by size:
3024 1800 281
AgeI A'CCGG_T
Cuts at: 1550 1550
Size: 5105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1753 1753
Size: 5105
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 707 739 707
Size: 32 5073
AlwNI CAG_nnn'CTG
Cuts at: 849 1173 1612 1750 2410 3848 849
Size: 324 439 138 660 1438 2106
Fragments arranged by size:
2106 1438 660 439 324 138
ApaI G_GGCC'C
Cuts at: 858 858
Size: 5105
ApaLI G'TGCA_C
Cuts at: 3943 3943
Size: 5105
ApoI r'AATT_y
Cuts at: 2109 4458 4714 2109
Size: 2349 256 2500
Fragments arranged by size:
2500 2349 256
AscI GG'CGCG_CC
Cuts at: 129 129
Size: 5105
AseI AT'TA_AT
Cuts at: 20 20
Size: 5105
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
AvaI C'yCGr_G
Cuts at: 361 1824 2125 361
Size: 1463 301 3341
Fragments arranged by size:
3341 1463 301
AvrII C'CTAG_G
Cuts at: 633 633
Size: 5105
BamHI G'GATC_C
Cuts at: 2130 2130
Size: 5105
BanI G'GyrC_C
Cuts at: 2121 2535 2783 2818 2121
Size: 414 248 35 4408
Fragments arranged by size:
4408 414 248 35
BanII G_rGCy'C
Cuts at: 221 362 858 1923 2080 2119 3149 221
Size: 141 496 1065 157 39 1030 2177
Fragments arranged by size:
2177 1065 1030 496 157 141 39
BbeI G_GCGC'C
Cuts at: 2539 2787 2539
Size: 248 4857
BbsI GAAGACnn'nnnn_
Cuts at: 907 907
Size: 5105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 984 1018 984
Size: 34 5071
BciVI GTATCCnnnnn_n'
Cuts at: 3003 4059 5105 3003
Size: 1056 1046 3003
Fragments arranged by size:
3003 1056 1046
BclI T'GATC_A
Cuts at: 2625 2625
Size: 5105
BglI GCCn_nnn'nGGC
Cuts at: 352 1057 1897 352
Size: 705 840 3560
Fragments arranged by size:
3560 840 705
BglII A'GATC_T
Cuts at: 2620 4657 2620
Size: 2037 3068
BlpI GC'TnA_GC
Cuts at: 424 1024 2217 424
Size: 600 1193 3312
Fragments arranged by size:
3312 1193 600
Bme1580I G_kGCm'C
Cuts at: 858 2730 2823 3947 858
Size: 1872 93 1124 2016
Fragments arranged by size:
2016 1872 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1718 2317 2398 2733 4877 1718
Size: 599 81 335 2144 1946
Fragments arranged by size:
2144 1946 599 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 575 3472 575
Size: 2897 2208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 538 601 916 1018 1096 1390 1944 2043
Size: 99 63 315 102 78 294 554 99
Cuts at: 2043 439
Size: 3501
Fragments arranged by size:
3501 554 315 294 102 99 99 78
63
Bpu10I CC'TnA_GC
Cuts at: 497 1914 497
Size: 1417 3688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 2281 3160 3874 4172 643
Size: 1638 879 714 298 1576
Fragments arranged by size:
1638 1576 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 106 751 769 1064 4809 106
Size: 645 18 295 3745 402
Fragments arranged by size:
3745 645 402 295 18
BsaAI yAC'GTr
Cuts at: 2458 2458
Size: 5105
BsaBI GATnn'nnATC
Cuts at: 6 2619 4656 6
Size: 2613 2037 455
Fragments arranged by size:
2613 2037 455
BsaHI Gr'CG_yC
Cuts at: 2536 2784 3486 4982 2536
Size: 248 702 1496 2659
Fragments arranged by size:
2659 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1550 2294 2815 3493 3904 4051 4325 1550
Size: 744 521 678 411 147 274 2330
Fragments arranged by size:
2330 744 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 1441 1471 1792 1822 1910 1940 103
Size: 30 1308 30 321 30 88 30 3268
Fragments arranged by size:
3268 1308 321 88 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 760 991 1270 1273 1450 1462 1932 454
Size: 306 231 279 3 177 12 470 3627
Fragments arranged by size:
3627 470 306 279 231 177 12 3
BseYI C'CCAG_C
Cuts at: 122 857 1019 1279 1579 1612 2530 3288 3953
Size: 735 162 260 300 33 918 758 665
Cuts at: 3953 4361 122
Size: 408 866
Fragments arranged by size:
918 866 758 735 665 408 300 260
162 33
BsiEI CG_ry'CG
Cuts at: 104 249 2647 2693 3923 104
Size: 145 2398 46 1230 1286
Fragments arranged by size:
2398 1286 1230 145 46
BsiHKAI G_wGCw'C
Cuts at: 221 362 556 2119 2897 3087 3947 221
Size: 141 194 1563 778 190 860 1379
Fragments arranged by size:
1563 1379 860 778 194 190 141
BsiWI C'GTAC_G
Cuts at: 1687 1687
Size: 5105
Bsp1286I G_dGCh'C
Cuts at: 221 362 556 858 1923 2080 2119 2730 2823
Size: 141 194 302 1065 157 39 611 93
Cuts at: 2823 2897 3087 3149 3947 221
Size: 74 190 62 798 1379
Fragments arranged by size:
1379 1065 798 611 302 194 190 157
141 93 74 62 39
BspHI T'CATG_A
Cuts at: 771 3537 771
Size: 2766 2339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 1575 1796 1856 1885 2155 2369 2671 3052
Size: 1357 221 60 29 270 214 302 381
Cuts at: 3052 218
Size: 2271
Fragments arranged by size:
2271 1357 381 302 270 221 214 60
29
BsrBI CCG'CTC
Cuts at: 50 1098 1134 2039 3397 3451 4935 5100 50
Size: 1048 36 905 1358 54 1484 165 55
Fragments arranged by size:
1484 1358 1048 905 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 3017 3017
Size: 5105
BsrFI r'CCGG_y
Cuts at: 824 1109 1550 3103 3284 824
Size: 285 441 1553 181 2645
Fragments arranged by size:
2645 1553 441 285 181
BssHII G'CGCG_C
Cuts at: 129 402 460 129
Size: 273 58 4774
Fragments arranged by size:
4774 273 58
BssSI C'ACGA_G
Cuts at: 973 3376 4084 973
Size: 2403 708 1994
Fragments arranged by size:
2403 1994 708
BstAPI GCAn_nnn'nTGC
Cuts at: 600 849 2482 600
Size: 249 1633 3223
Fragments arranged by size:
3223 1633 249
BstBI TT'CG_AA
Cuts at: 3466 3466
Size: 5105
BstEII G'GTnAC_C
Cuts at: 481 2031 481
Size: 1550 3555
BstYI r'GATC_y
Cuts at: 1 1223 2130 2597 2620 2955 3201 3507 3519
Size: 1222 907 467 23 335 246 306 12
Cuts at: 3519 3605 4657 1
Size: 86 1052 449
Fragments arranged by size:
1222 1052 907 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1302 1430 1496 1803 107
Size: 1195 128 66 307 3409
Fragments arranged by size:
3409 1195 307 128 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3055 3236 3399 3055
Size: 181 163 4761
Fragments arranged by size:
4761 181 163
BtsI GCAGTG_nn'
Cuts at: 554 1812 1995 2007 2348 554
Size: 1258 183 12 341 3311
Fragments arranged by size:
3311 1258 341 183 12
DraI TTT'AAA
Cuts at: 2104 2104
Size: 5105
DraIII CAC_nnn'GTG
Cuts at: 1339 1339
Size: 5105
DrdI GACnn_nn'nnGTC
Cuts at: 2029 2811 4155 2029
Size: 782 1344 2979
Fragments arranged by size:
2979 1344 782
EaeI y'GGCC_r
Cuts at: 614 1142 1305 1427 1721 2690 2864 3255 3282
Size: 528 163 122 294 969 174 391 27
Cuts at: 3282 4627 614
Size: 1345 1092
Fragments arranged by size:
1345 1092 969 528 391 294 174 163
122 27
EagI C'GGCC_G
Cuts at: 2690 2690
Size: 5105
EarI CTCTTCn'nnn_
Cuts at: 302 1956 3128 3338 302
Size: 1654 1172 210 2069
Fragments arranged by size:
2069 1654 1172 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 847 1138 1946 3313 4055 4201 4915 847
Size: 291 808 1367 742 146 714 1037
Fragments arranged by size:
1367 1037 808 742 714 291 146
EcoICRI GAG'CTC
Cuts at: 219 360 2117 219
Size: 141 1757 3207
Fragments arranged by size:
3207 1757 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 439 529 538 601 640 916 1018 1096
Size: 113 90 9 63 39 276 102 78
Cuts at: 1096 1390 1594 1944 2043 2930 3362 3715 4377
Size: 294 204 350 99 887 432 353 662
Cuts at: 4377 326
Size: 1054
Fragments arranged by size:
1054 887 662 432 353 350 294 276
204 113 102 99 90 78 63 39
9
EcoNI CCTnn'n_nnAGG
Cuts at: 637 637
Size: 5105
EcoO109I rG'GnC_Cy
Cuts at: 207 513 1830 1874 2244 207
Size: 306 1317 44 370 3068
Fragments arranged by size:
3068 1317 370 306 44
EcoRI G'AATT_C
Cuts at: 2109 2109
Size: 5105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 5105
FspI TGC'GCA
Cuts at: 1471 1729 2886 1471
Size: 258 1157 3690
Fragments arranged by size:
3690 1157 258
HaeII r_GCGC'y
Cuts at: 768 1521 2539 2787 4017 4788 768
Size: 753 1018 248 1230 771 1085
Fragments arranged by size:
1230 1085 1018 771 753 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 480 512 997 1029 1477 1509 1885
Size: 32 345 32 485 32 448 32 376
Cuts at: 1885 1917 2947 2977 2979 3009 103
Size: 32 1030 30 2 30 2199
Fragments arranged by size:
2199 1030 485 448 376 345 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2144 2144
Size: 5105
HindIII A'AGCT_T
Cuts at: 619 1063 619
Size: 444 4661
KasI G'GCGC_C
Cuts at: 2535 2783 2535
Size: 248 4857
KpnI G_GTAC'C
Cuts at: 2125 2125
Size: 5105
MfeI C'AATT_G
Cuts at: 1503 1503
Size: 5105
MluI A'CGCG_T
Cuts at: 4538 4538
Size: 5105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3864 4048 3864
Size: 184 4921
MscI TGG'CCA
Cuts at: 616 1723 2866 616
Size: 1107 1143 2855
Fragments arranged by size:
2855 1143 1107
MslI CAynn'nnrTG
Cuts at: 3094 3221 3094
Size: 127 4978
MspA1I CmG'CkG
Cuts at: 126 143 168 417 1044 1173 1837 1910 2216
Size: 17 25 249 627 129 664 73 306
Cuts at: 2216 2530 2890 3674 3919 126
Size: 314 360 784 245 1312
Fragments arranged by size:
1312 784 664 627 360 314 306 249
245 129 73 25 17
NaeI GCC'GGC
Cuts at: 826 1111 3286 826
Size: 285 2175 2645
Fragments arranged by size:
2645 2175 285
NarI GG'CG_CC
Cuts at: 2536 2784 2536
Size: 248 4857
NcoI C'CATG_G
Cuts at: 107 1496 107
Size: 1389 3716
NgoMIV G'CCGG_C
Cuts at: 824 1109 3284 824
Size: 285 2175 2645
Fragments arranged by size:
2645 2175 285
NspI r_CATG'y
Cuts at: 2158 2658 4261 2158
Size: 500 1603 3002
Fragments arranged by size:
3002 1603 500
PciI A'CATG_T
Cuts at: 4257 4257
Size: 5105
PflMI CCAn_nnn'nTGG
Cuts at: 1497 1497
Size: 5105
PmeI GTTT'AAAC
Cuts at: 2104 2104
Size: 5105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 5073
PpuMI rG'GwC_Cy
Cuts at: 207 513 1830 1874 207
Size: 306 1317 44 3438
Fragments arranged by size:
3438 1317 306 44
PspOMI G'GGCC_C
Cuts at: 854 854
Size: 5105
PstI C_TGCA'G
Cuts at: 197 215 416 701 845 986 1608 1819 2092
Size: 18 201 285 144 141 622 211 273
Cuts at: 2092 2152 2837 197
Size: 60 685 2465
Fragments arranged by size:
2465 685 622 285 273 211 201 144
141 60 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5105
PvuII CAG'CTG
Cuts at: 417 1044 1173 2530 2890 417
Size: 627 129 1357 360 2632
Fragments arranged by size:
2632 1357 627 360 129
RsrII CG'GwC_CG
Cuts at: 3300 3300
Size: 5105
SacI G_AGCT'C
Cuts at: 221 362 2119 221
Size: 141 1757 3207
Fragments arranged by size:
3207 1757 141
SalI G'TCGA_C
Cuts at: 2142 2142
Size: 5105
SanDI GG'GwC_CC
Cuts at: 1830 1830
Size: 5105
SapI GCTCTTCn'nnn_
Cuts at: 1956 3128 3338 1956
Size: 1172 210 3723
Fragments arranged by size:
3723 1172 210
SbfI CC_TGCA'GG
Cuts at: 215 2152 215
Size: 1937 3168
ScaI AGT'ACT
Cuts at: 4622 4622
Size: 5105
SexAI A'CCwGG_T
Cuts at: 869 956 1640 869
Size: 87 684 4334
Fragments arranged by size:
4334 684 87
SfcI C'TryA_G
Cuts at: 33 193 211 412 697 738 841 982 1604
Size: 160 18 201 285 41 103 141 622
Cuts at: 1604 1815 2088 2148 2833 3801 3992 33
Size: 211 273 60 685 968 191 1146
Fragments arranged by size:
1146 968 685 622 285 273 211 201
191 160 141 103 60 41 18
SfoI GGC'GCC
Cuts at: 2537 2785 2537
Size: 248 4857
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
SmaI CCC'GGG
Cuts at: 2127 2127
Size: 5105
SmlI C'TyrA_G
Cuts at: 622 2260 3175 3889 4151 622
Size: 1638 915 714 262 1576
Fragments arranged by size:
1638 1576 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5105
SphI G_CATG'C
Cuts at: 2158 2658 2158
Size: 500 4605
StyI C'CwwG_G
Cuts at: 107 395 536 633 1052 1496 2239 2593 107
Size: 288 141 97 419 444 743 354 2619
Fragments arranged by size:
2619 743 444 419 354 288 141 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1636 2688 2821 3280 3489 1636
Size: 1052 133 459 209 3252
Fragments arranged by size:
3252 1052 459 209 133
TatI w'GTAC_w
Cuts at: 1841 4620 1841
Size: 2779 2326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 795 827 1792 1824 1910 1942 4622 4654 795
Size: 32 965 32 86 32 2680 32 1246
Fragments arranged by size:
2680 1246 965 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 117 2902 117
Size: 2785 2320
XbaI T'CTAG_A
Cuts at: 2136 2136
Size: 5105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1336 1731 2600 1336
Size: 395 869 3841
Fragments arranged by size:
3841 869 395
XmaI C'CCGG_G
Cuts at: 2125 2125
Size: 5105
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI BaeI BbvCI BfrBI
BmgBI BmtI BsgI BsmI BsmBI BspEI BsrGI BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI