Restriction map for
pF1KB4032
(Circular) (Six-base) MAPSORT of: pF1KB4032.seq Check: 3886 from: 1 to: 6515
pF1KB4032 6515 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1866 1866
Size: 6515
AccI GT'mk_AC
Cuts at: 1186 1514 1963 3553 1186
Size: 328 449 1590 4148
Fragments arranged by size:
4148 1590 449 328
Acc65I G'GTAC_C
Cuts at: 3531 3531
Size: 6515
AclI AA'CG_TT
Cuts at: 6355 6355
Size: 6515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 502 1250 1673 1902 2156 2783 4340 4772
Size: 218 748 423 229 254 627 1557 432
Cuts at: 4772 5519 5787 284
Size: 747 268 1012
Fragments arranged by size:
1557 1012 748 747 627 432 423 268
254 229 218
AfeI AGC'GCT
Cuts at: 589 589
Size: 6515
AflIII A'CryG_T
Cuts at: 2502 2694 3867 4971 5948 2502
Size: 192 1173 1104 977 3069
Fragments arranged by size:
3069 1173 1104 977 192
AgeI A'CCGG_T
Cuts at: 4903 4903
Size: 6515
AhdI GACnn_n'nnGTC
Cuts at: 894 894
Size: 6515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 650 3227 3237 650
Size: 2577 10 3928
Fragments arranged by size:
3928 2577 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2101 2133 2101
Size: 32 6483
AlwNI CAG_nnn'CTG
Cuts at: 907 1293 2152 2164 2452 3820 5387 907
Size: 386 859 12 288 1368 1567 2035
Fragments arranged by size:
2035 1567 1368 859 386 288 12
ApaI G_GGCC'C
Cuts at: 2771 2771
Size: 6515
ApaLI G'TGCA_C
Cuts at: 5285 5285
Size: 6515
ApoI r'AATT_y
Cuts at: 2201 2893 2938 3519 5868 6124 2201
Size: 692 45 581 2349 256 2592
Fragments arranged by size:
2592 2349 692 581 256 45
AscI GG'CGCG_CC
Cuts at: 3424 3424
Size: 6515
AseI AT'TA_AT
Cuts at: 20 1652 20
Size: 1632 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6515
AvaI C'yCGr_G
Cuts at: 3535 3535
Size: 6515
AvrII C'CTAG_G
Cuts at: 490 490
Size: 6515
BamHI G'GATC_C
Cuts at: 417 3540 417
Size: 3123 3392
BanI G'GyrC_C
Cuts at: 1977 3531 3945 4193 4228 1977
Size: 1554 414 248 35 4264
Fragments arranged by size:
4264 1554 414 248 35
BanII G_rGCy'C
Cuts at: 809 2513 2771 3529 4559 809
Size: 1704 258 758 1030 2765
Fragments arranged by size:
2765 1704 1030 758 258
BbeI G_GCGC'C
Cuts at: 1981 3949 4197 1981
Size: 1968 248 4299
Fragments arranged by size:
4299 1968 248
BbsI GAAGACnn'nnnn_
Cuts at: 2783 3401 2783
Size: 618 5897
BbvCI CC'TCA_GC
Cuts at: 1048 1465 1048
Size: 417 6098
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1767 1801 90
Size: 34 1643 34 4804
Fragments arranged by size:
4804 1643 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4413 5174 6515 4413
Size: 761 1341 4413
Fragments arranged by size:
4413 1341 761
BclI T'GATC_A
Cuts at: 4035 4035
Size: 6515
BglI GCCn_nnn'nGGC
Cuts at: 383 1209 2766 383
Size: 826 1557 4132
Fragments arranged by size:
4132 1557 826
BglII A'GATC_T
Cuts at: 409 1306 1380 4030 6067 409
Size: 897 74 2650 2037 857
Fragments arranged by size:
2650 2037 897 857 74
BlpI GC'TnA_GC
Cuts at: 638 689 908 3627 638
Size: 51 219 2719 3526
Fragments arranged by size:
3526 2719 219 51
Bme1580I G_kGCm'C
Cuts at: 2771 4140 4233 5289 2771
Size: 1369 93 1056 3997
Fragments arranged by size:
3997 1369 1056 93
BmgBI CAC'GTC
Cuts at: 216 216
Size: 6515
BmrI ACTGGGnnnn_n'
Cuts at: 1433 2470 3727 3808 4143 6287 1433
Size: 1037 1257 81 335 2144 1661
Fragments arranged by size:
2144 1661 1257 1037 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2436 2544 4882 2436
Size: 108 2338 4069
Fragments arranged by size:
4069 2338 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1817 2591 2641 2734 179
Size: 1638 774 50 93 3960
Fragments arranged by size:
3960 1638 774 93 50
Bpu10I CC'TnA_GC
Cuts at: 1048 1465 1589 1048
Size: 417 124 5974
Fragments arranged by size:
5974 417 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1191 3691 4570 5062 5360 844
Size: 347 2500 879 492 298 1999
Fragments arranged by size:
2500 1999 879 492 347 298
BsaI GGTCTCn'nnnn_
Cuts at: 2407 2532 2823 6219 2407
Size: 125 291 3396 2703
Fragments arranged by size:
3396 2703 291 125
BsaAI yAC'GTr
Cuts at: 723 3457 3868 723
Size: 2734 411 3370
Fragments arranged by size:
3370 2734 411
BsaBI GATnn'nnATC
Cuts at: 6 1118 4029 6066 6
Size: 1112 2911 2037 455
Fragments arranged by size:
2911 2037 1112 455
BsaHI Gr'CG_yC
Cuts at: 1978 3946 4194 4896 6392 1978
Size: 1968 248 702 1496 2101
Fragments arranged by size:
2101 1968 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3704 4225 4903 5177 5324 5735 3704
Size: 521 678 274 147 411 4484
Fragments arranged by size:
4484 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1815 1845 3161 3191 1815
Size: 30 1316 30 5139
Fragments arranged by size:
5139 1316 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2731 2731
Size: 6515
BseYI C'CCAG_C
Cuts at: 557 1127 1795 1886 3465 3940 4698 5275 5771
Size: 570 668 91 1579 475 758 577 496
Cuts at: 5771 557
Size: 1301
Fragments arranged by size:
1579 1301 758 668 577 570 496 475
91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2026 3050 2026
Size: 1024 5491
BsiEI CG_ry'CG
Cuts at: 104 4057 4103 5311 104
Size: 3953 46 1208 1308
Fragments arranged by size:
3953 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 711 809 3256 3529 4307 4497 5289 711
Size: 98 2447 273 778 190 792 1937
Fragments arranged by size:
2447 1937 792 778 273 190 98
BsmI GAATG_Cn'
Cuts at: 972 1434 2574 972
Size: 462 1140 4913
Fragments arranged by size:
4913 1140 462
BsmBI CGTCTCn'nnnn_
Cuts at: 206 206
Size: 6515
Bsp1286I G_dGCh'C
Cuts at: 711 809 2513 2771 3256 3529 4140 4233 4307
Size: 98 1704 258 485 273 611 93 74
Cuts at: 4307 4497 4559 5289 711
Size: 190 62 730 1937
Fragments arranged by size:
1937 1704 730 611 485 273 258 190
98 93 74 62
BspEI T'CCGG_A
Cuts at: 5735 5735
Size: 6515
BspHI T'CATG_A
Cuts at: 369 5691 369
Size: 5322 1193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1866 2805 3565 3779 4081 4462 1866
Size: 939 760 214 302 381 3919
Fragments arranged by size:
3919 939 760 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4807 4861 6345 6510 50
Size: 4757 54 1484 165 55
Fragments arranged by size:
4757 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 750 4427 750
Size: 3677 2838
BsrFI r'CCGG_y
Cuts at: 1055 4513 4694 4903 1055
Size: 3458 181 209 2667
Fragments arranged by size:
3458 2667 209 181
BsrGI T'GTAC_A
Cuts at: 2949 2949
Size: 6515
BssHII G'CGCG_C
Cuts at: 3424 3424
Size: 6515
BssSI C'ACGA_G
Cuts at: 4786 5144 4786
Size: 358 6157
BstAPI GCAn_nnn'nTGC
Cuts at: 567 2004 3892 567
Size: 1437 1888 3190
Fragments arranged by size:
3190 1888 1437
BstBI TT'CG_AA
Cuts at: 4876 4876
Size: 6515
BstXI CCAn_nnnn'nTGG
Cuts at: 1351 1351
Size: 6515
BstYI r'GATC_y
Cuts at: 1 222 409 417 498 1306 1380 3540 4007
Size: 221 187 8 81 808 74 2160 467
Cuts at: 4007 4030 4365 4611 5623 5709 5721 6067 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2160 1012 808 467 449 346 335 246
221 187 86 81 74 23 12 8
BstZ17I GTA'TAC
Cuts at: 1964 1964
Size: 6515
Bsu36I CC'TnA_GG
Cuts at: 2627 2627
Size: 6515
BtgI C'CryG_G
Cuts at: 1742 1934 2408 1742
Size: 192 474 5849
Fragments arranged by size:
5849 474 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4465 4646 4809 4465
Size: 181 163 6171
Fragments arranged by size:
6171 181 163
BtsI GCAGTG_nn'
Cuts at: 908 1291 1572 3758 908
Size: 383 281 2186 3665
Fragments arranged by size:
3665 2186 383 281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1136 1171 3443 3478 1136
Size: 35 2272 35 4173
Fragments arranged by size:
4173 2272 35 35
DraI TTT'AAA
Cuts at: 428 3514 428
Size: 3086 3429
DraIII CAC_nnn'GTG
Cuts at: 3251 3251
Size: 6515
DrdI GACnn_nn'nnGTC
Cuts at: 4221 5079 4221
Size: 858 5657
EaeI y'GGCC_r
Cuts at: 1812 2209 2405 3506 4100 4274 4665 4692 6037
Size: 397 196 1101 594 174 391 27 1345
Cuts at: 6037 1812
Size: 2290
Fragments arranged by size:
2290 1345 1101 594 397 391 196 174
27
EagI C'GGCC_G
Cuts at: 4100 4100
Size: 6515
EarI CTCTTCn'nnn_
Cuts at: 727 3017 4538 4748 727
Size: 2290 1521 210 2494
Fragments arranged by size:
2494 2290 1521 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4723 5033 5179 6325 4723
Size: 310 146 1146 4913
Fragments arranged by size:
4913 1146 310 146
EcoICRI GAG'CTC
Cuts at: 807 3527 807
Size: 2720 3795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 284 502 1250 1673 1817 1902 2156 2591
Size: 105 218 748 423 144 85 254 435
Cuts at: 2591 2641 2734 2783 4340 4772 5519 5787 179
Size: 50 93 49 1557 432 747 268 907
Fragments arranged by size:
1557 907 748 747 435 432 423 268
254 218 144 105 93 85 50 49
EcoNI CCTnn'n_nnAGG
Cuts at: 925 2606 925
Size: 1681 4834
EcoO109I rG'GnC_Cy
Cuts at: 1168 2353 2767 3654 1168
Size: 1185 414 887 4029
Fragments arranged by size:
4029 1185 887 414
EcoRI G'AATT_C
Cuts at: 2201 3519 2201
Size: 1318 5197
EcoRV GAT'ATC
Cuts at: 1575 1575
Size: 6515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4534 4534
Size: 6515
FspI TGC'GCA
Cuts at: 4296 4296
Size: 6515
HaeII r_GCGC'y
Cuts at: 591 1981 3949 4197 5219 6198 591
Size: 1390 1968 248 1022 979 908
Fragments arranged by size:
1968 1390 1022 979 908 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 880 912 1815 1847 2010 2042 4357 4387 4389
Size: 32 903 32 163 32 2315 30 2
Cuts at: 4389 4419 880
Size: 30 2976
Fragments arranged by size:
2976 2315 903 163 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1727 2018 3554 1727
Size: 291 1536 4688
Fragments arranged by size:
4688 1536 291
HindIII A'AGCT_T
Cuts at: 2377 2377
Size: 6515
KasI G'GCGC_C
Cuts at: 1977 3945 4193 1977
Size: 1968 248 4299
Fragments arranged by size:
4299 1968 248
KpnI G_GTAC'C
Cuts at: 3535 3535
Size: 6515
MfeI C'AATT_G
Cuts at: 565 565
Size: 6515
MluI A'CGCG_T
Cuts at: 5948 5948
Size: 6515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5186 5370 5186
Size: 184 6331
MscI TGG'CCA
Cuts at: 1814 4276 1814
Size: 2462 4053
MslI CAynn'nnrTG
Cuts at: 4504 4631 4504
Size: 127 6388
MspA1I CmG'CkG
Cuts at: 1464 3175 3626 3940 4300 5313 5558 1464
Size: 1711 451 314 360 1013 245 2421
Fragments arranged by size:
2421 1711 1013 451 360 314 245
NaeI GCC'GGC
Cuts at: 4696 4696
Size: 6515
NarI GG'CG_CC
Cuts at: 1978 3946 4194 1978
Size: 1968 248 4299
Fragments arranged by size:
4299 1968 248
NcoI C'CATG_G
Cuts at: 1742 2408 1742
Size: 666 5849
NgoMIV G'CCGG_C
Cuts at: 4694 4694
Size: 6515
NspI r_CATG'y
Cuts at: 2506 2595 2698 2793 3204 3568 4068 4975 2506
Size: 89 103 95 411 364 500 907 4046
Fragments arranged by size:
4046 907 500 411 364 103 95 89
PciI A'CATG_T
Cuts at: 2502 2694 4971 2502
Size: 192 2277 4046
Fragments arranged by size:
4046 2277 192
PflMI CCAn_nnn'nTGG
Cuts at: 1935 1935
Size: 6515
PmeI GTTT'AAAC
Cuts at: 3514 3514
Size: 6515
PmlI CAC'GTG
Cuts at: 723 723
Size: 6515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5654 5686 5654
Size: 32 6483
PpuMI rG'GwC_Cy
Cuts at: 1168 2353 1168
Size: 1185 5330
PspOMI G'GGCC_C
Cuts at: 2767 2767
Size: 6515
PstI C_TGCA'G
Cuts at: 903 938 1164 3216 3562 4247 903
Size: 35 226 2052 346 685 3171
Fragments arranged by size:
3171 2052 685 346 226 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6515
PvuII CAG'CTG
Cuts at: 3940 4300 3940
Size: 360 6155
RsrII CG'GwC_CG
Cuts at: 4710 4710
Size: 6515
SacI G_AGCT'C
Cuts at: 809 3529 809
Size: 2720 3795
SalI G'TCGA_C
Cuts at: 3552 3552
Size: 6515
SapI GCTCTTCn'nnn_
Cuts at: 3017 4538 4748 3017
Size: 1521 210 4784
Fragments arranged by size:
4784 1521 210
SbfI CC_TGCA'GG
Cuts at: 3562 3562
Size: 6515
ScaI AGT'ACT
Cuts at: 6032 6032
Size: 6515
SfcI C'TryA_G
Cuts at: 33 899 934 1081 1160 3192 3212 3558 4243
Size: 866 35 147 79 2032 20 346 685
Cuts at: 4243 5236 5427 33
Size: 993 191 1121
Fragments arranged by size:
2032 1121 993 866 685 346 191 147
79 35 20
SfoI GGC'GCC
Cuts at: 1979 3947 4195 1979
Size: 1968 248 4299
Fragments arranged by size:
4299 1968 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6515
SmaI CCC'GGG
Cuts at: 3537 3537
Size: 6515
SmlI C'TyrA_G
Cuts at: 859 1206 3670 4585 5077 5339 859
Size: 347 2464 915 492 262 2035
Fragments arranged by size:
2464 2035 915 492 347 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6515
SphI G_CATG'C
Cuts at: 2793 3568 4068 2793
Size: 775 500 5240
Fragments arranged by size:
5240 775 500
SspI AAT'ATT
Cuts at: 2370 2370
Size: 6515
StuI AGG'CCT
Cuts at: 1212 2316 2612 1212
Size: 1104 296 5115
Fragments arranged by size:
5115 1104 296
StyI C'CwwG_G
Cuts at: 490 1250 1395 1742 2311 2408 3649 4003 490
Size: 760 145 347 569 97 1241 354 3002
Fragments arranged by size:
3002 1241 760 569 354 347 145 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2117 4098 4231 4690 4899 2117
Size: 1981 133 459 209 3733
Fragments arranged by size:
3733 1981 459 209 133
TatI w'GTAC_w
Cuts at: 2575 2949 6030 2575
Size: 374 3081 3060
Fragments arranged by size:
3081 3060 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 168 200 1045 1077 1893 1925 2873 2905 3161
Size: 32 845 32 816 32 948 32 256
Cuts at: 3161 3193 6032 6064 168
Size: 32 2839 32 619
Fragments arranged by size:
2839 948 845 816 619 256 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4312 4312
Size: 6515
XbaI T'CTAG_A
Cuts at: 3546 3546
Size: 6515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2406 3265 4010 2134
Size: 272 859 745 4639
Fragments arranged by size:
4639 859 745 272
XmaI C'CCGG_G
Cuts at: 3535 3535
Size: 6515
XmnI GAAnn'nnTTC
Cuts at: 2201 2201
Size: 6515
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI BaeI BfrBI BmtI BsiWI BstEII
ClaI FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI