Restriction map for
pF1KB4033
(Circular) (Six-base) MAPSORT of: pF1KB4033.seq Check: 7727 from: 1 to: 6899
pF1KB4033 6899 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 2051 2851 2051
Size: 800 6099
AccI GT'mk_AC
Cuts at: 505 2752 3937 505
Size: 2247 1185 3467
Fragments arranged by size:
3467 2247 1185
Acc65I G'GTAC_C
Cuts at: 3915 3915
Size: 6899
AclI AA'CG_TT
Cuts at: 2105 6739 2105
Size: 4634 2265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 704 1018 1154 1691 2842 3179 3469 4724
Size: 537 314 136 537 1151 337 290 1255
Cuts at: 4724 5156 5509 6171 167
Size: 432 353 662 895
Fragments arranged by size:
1255 1151 895 662 537 537 432 353
337 314 290 136
AflII C'TTAA_G
Cuts at: 2053 2053
Size: 6899
AflIII A'CryG_T
Cuts at: 4251 6051 6332 4251
Size: 1800 281 4818
Fragments arranged by size:
4818 1800 281
AgeI A'CCGG_T
Cuts at: 2186 2186
Size: 6899
AleI CACnn'nnGTG
Cuts at: 329 3660 329
Size: 3331 3568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3706 3706
Size: 6899
AlwNI CAG_nnn'CTG
Cuts at: 1493 3642 4204 5642 1493
Size: 2149 562 1438 2750
Fragments arranged by size:
2750 2149 1438 562
ApaLI G'TGCA_C
Cuts at: 5737 5737
Size: 6899
ApoI r'AATT_y
Cuts at: 135 178 226 2137 3903 6252 6508 135
Size: 43 48 1911 1766 2349 256 526
Fragments arranged by size:
2349 1911 1766 526 256 48 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 6899
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6899
AvaI C'yCGr_G
Cuts at: 3068 3919 3068
Size: 851 6048
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1218 1251 1218
Size: 33 6866
BamHI G'GATC_C
Cuts at: 1651 2192 3924 1651
Size: 541 1732 4626
Fragments arranged by size:
4626 1732 541
BanI G'GyrC_C
Cuts at: 949 1498 2265 3915 4329 4577 4612 949
Size: 549 767 1650 414 248 35 3236
Fragments arranged by size:
3236 1650 767 549 414 248 35
BanII G_rGCy'C
Cuts at: 3913 4943 3913
Size: 1030 5869
BbeI G_GCGC'C
Cuts at: 4333 4581 4333
Size: 248 6651
BbsI GAAGACnn'nnnn_
Cuts at: 585 2899 2942 3492 3727 3830 3844 3859 585
Size: 2314 43 550 235 103 14 15 3625
Fragments arranged by size:
3625 2314 550 235 103 43 15 14
BbvCI CC'TCA_GC
Cuts at: 1097 1097
Size: 6899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6865
BciVI GTATCCnnnnn_n'
Cuts at: 572 2651 4797 5853 6899 572
Size: 2079 2146 1056 1046 572
Fragments arranged by size:
2146 2079 1056 1046 572
BclI T'GATC_A
Cuts at: 2609 2961 3593 4419 2609
Size: 352 632 826 5089
Fragments arranged by size:
5089 826 632 352
BfrBI ATG'CAT
Cuts at: 1547 1547
Size: 6899
BglI GCCn_nnn'nGGC
Cuts at: 2836 2836
Size: 6899
BglII A'GATC_T
Cuts at: 1589 1596 4414 6451 1589
Size: 7 2818 2037 2037
Fragments arranged by size:
2818 2037 2037 7
BlpI GC'TnA_GC
Cuts at: 4011 4011
Size: 6899
Bme1580I G_kGCm'C
Cuts at: 1346 1501 3685 4524 4617 5741 1346
Size: 155 2184 839 93 1124 2504
Fragments arranged by size:
2504 2184 1124 839 155 93
BmrI ACTGGGnnnn_n'
Cuts at: 415 1419 1503 2906 4111 4192 4527 6671 415
Size: 1004 84 1403 1205 81 335 2144 643
Fragments arranged by size:
2144 1403 1205 1004 643 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3418 5266 3418
Size: 1848 5051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1329 2101 2899 1329
Size: 772 798 5329
Fragments arranged by size:
5329 798 772
Bpu10I CC'TnA_GC
Cuts at: 1097 1715 2782 3008 1097
Size: 618 1067 226 4988
Fragments arranged by size:
4988 1067 618 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4954 5668 5966 4075
Size: 879 714 298 5008
Fragments arranged by size:
5008 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2609 3793 6603 2609
Size: 1184 2810 2905
Fragments arranged by size:
2905 2810 1184
BsaAI yAC'GTr
Cuts at: 1465 4252 1465
Size: 2787 4112
BsaBI GATnn'nnATC
Cuts at: 6 4413 6450 6
Size: 4407 2037 455
Fragments arranged by size:
4407 2037 455
BsaHI Gr'CG_yC
Cuts at: 2048 2848 4330 4578 5280 6776 2048
Size: 800 1482 248 702 1496 2171
Fragments arranged by size:
2171 1496 1482 800 702 248
BsaWI w'CCGG_w
Cuts at: 2186 4088 4609 5287 5698 5845 6119 2186
Size: 1902 521 678 411 147 274 2966
Fragments arranged by size:
2966 1902 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 768 798 768
Size: 30 6869
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1313 1549 2887 1313
Size: 236 1338 5325
Fragments arranged by size:
5325 1338 236
BseYI C'CCAG_C
Cuts at: 1345 4324 5082 5747 6155 1345
Size: 2979 758 665 408 2089
Fragments arranged by size:
2979 2089 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2588 2588
Size: 6899
BsiEI CG_ry'CG
Cuts at: 104 4441 4487 5717 104
Size: 4337 46 1230 1286
Fragments arranged by size:
4337 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3913 4691 4881 5741 3913
Size: 778 190 860 5071
Fragments arranged by size:
5071 860 778 190
BsmI GAATG_Cn'
Cuts at: 202 2507 2774 3651 202
Size: 2305 267 877 3450
Fragments arranged by size:
3450 2305 877 267
Bsp1286I G_dGCh'C
Cuts at: 1346 1501 3685 3913 4524 4617 4691 4881 4943
Size: 155 2184 228 611 93 74 190 62
Cuts at: 4943 5741 1346
Size: 798 2504
Fragments arranged by size:
2504 2184 798 611 228 190 155 93
74 62
BspHI T'CATG_A
Cuts at: 5331 5331
Size: 6899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3949 4163 4465 4846 3949
Size: 214 302 381 6002
Fragments arranged by size:
6002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5191 5245 6729 6894 50
Size: 5141 54 1484 165 55
Fragments arranged by size:
5141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1775 4811 1775
Size: 3036 3863
BsrFI r'CCGG_y
Cuts at: 2186 4897 5078 2186
Size: 2711 181 4007
Fragments arranged by size:
4007 2711 181
BsrGI T'GTAC_A
Cuts at: 2951 2951
Size: 6899
BssSI C'ACGA_G
Cuts at: 5170 5878 5170
Size: 708 6191
BstAPI GCAn_nnn'nTGC
Cuts at: 3107 4276 3107
Size: 1169 5730
BstBI TT'CG_AA
Cuts at: 2736 5260 2736
Size: 2524 4375
BstXI CCAn_nnnn'nTGG
Cuts at: 1960 2896 2910 1960
Size: 936 14 5949
Fragments arranged by size:
5949 936 14
BstYI r'GATC_y
Cuts at: 1 714 929 1589 1596 1651 1935 2192 2673
Size: 713 215 660 7 55 284 257 481
Cuts at: 2673 3292 3322 3924 4391 4414 4749 4995 5301
Size: 619 30 602 467 23 335 246 306
Cuts at: 5301 5313 5399 6451 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 713 660 619 602 481 467 449
335 306 284 257 246 215 86 55
30 23 12 7
Bsu36I CC'TnA_GG
Cuts at: 1387 1387
Size: 6899
BtgI C'CryG_G
Cuts at: 369 1832 2071 369
Size: 1463 239 5197
Fragments arranged by size:
5197 1463 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1538 4849 5030 5193 1538
Size: 3311 181 163 3244
Fragments arranged by size:
3311 3244 181 163
BtsI GCAGTG_nn'
Cuts at: 1346 2846 2874 3387 4142 1346
Size: 1500 28 513 755 4103
Fragments arranged by size:
4103 1500 755 513 28
DraI TTT'AAA
Cuts at: 2028 3443 3898 2028
Size: 1415 455 5029
Fragments arranged by size:
5029 1415 455
DraIII CAC_nnn'GTG
Cuts at: 1642 3565 1642
Size: 1923 4976
DrdI GACnn_nn'nnGTC
Cuts at: 1108 4605 5949 1108
Size: 3497 1344 2058
Fragments arranged by size:
3497 2058 1344
EaeI y'GGCC_r
Cuts at: 431 522 1829 1899 4484 4658 5049 5076 6421
Size: 91 1307 70 2585 174 391 27 1345
Cuts at: 6421 431
Size: 909
Fragments arranged by size:
2585 1345 1307 909 391 174 91 70
27
EagI C'GGCC_G
Cuts at: 4484 4484
Size: 6899
EarI CTCTTCn'nnn_
Cuts at: 146 2027 2598 3445 3617 4922 5132 146
Size: 1881 571 847 172 1305 210 1913
Fragments arranged by size:
1913 1881 1305 847 571 210 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1576 2932 5107 5849 5995 6709 1576
Size: 1356 2175 742 146 714 1766
Fragments arranged by size:
2175 1766 1356 742 714 146
EcoICRI GAG'CTC
Cuts at: 3911 3911
Size: 6899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 704 1018 1154 1329 1691 2101 2842 2899
Size: 537 314 136 175 362 410 741 57
Cuts at: 2899 3179 3469 4724 5156 5509 6171 167
Size: 280 290 1255 432 353 662 895
Fragments arranged by size:
1255 895 741 662 537 432 410 362
353 314 290 280 175 136 57
EcoO109I rG'GnC_Cy
Cuts at: 977 1413 4038 977
Size: 436 2625 3838
Fragments arranged by size:
3838 2625 436
EcoRI G'AATT_C
Cuts at: 3903 3903
Size: 6899
EcoRV GAT'ATC
Cuts at: 2694 2694
Size: 6899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1282 1671 2847 3243 4918 1282
Size: 389 1176 396 1675 3263
Fragments arranged by size:
3263 1675 1176 396 389
FspI TGC'GCA
Cuts at: 2984 4680 2984
Size: 1696 5203
HaeII r_GCGC'y
Cuts at: 1019 4333 4581 5811 6582 1019
Size: 3314 248 1230 771 1336
Fragments arranged by size:
3314 1336 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 768 800 857 889 1574 1581 1606
Size: 32 249 32 57 32 685 7 25
Cuts at: 1606 1613 2683 2715 4741 4771 4773 4803 487
Size: 7 1070 32 2026 30 2 30 2583
Fragments arranged by size:
2583 2026 1070 685 249 57 32 32
32 32 30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 345 1035 1617 3938 345
Size: 690 582 2321 3306
Fragments arranged by size:
3306 2321 690 582
KasI G'GCGC_C
Cuts at: 4329 4577 4329
Size: 248 6651
KpnI G_GTAC'C
Cuts at: 3919 3919
Size: 6899
MfeI C'AATT_G
Cuts at: 473 2301 473
Size: 1828 5071
MluI A'CGCG_T
Cuts at: 6332 6332
Size: 6899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 3145 3413 5658 5842 701
Size: 2444 268 2245 184 1758
Fragments arranged by size:
2444 2245 1758 268 184
MscI TGG'CCA
Cuts at: 433 1831 1901 4660 433
Size: 1398 70 2759 2672
Fragments arranged by size:
2759 2672 1398 70
MslI CAynn'nnrTG
Cuts at: 329 880 2908 3002 3660 3832 4888 5015 329
Size: 551 2028 94 658 172 1056 127 2213
Fragments arranged by size:
2213 2028 1056 658 551 172 127 94
MspA1I CmG'CkG
Cuts at: 1349 4010 4324 4684 5468 5713 1349
Size: 2661 314 360 784 245 2535
Fragments arranged by size:
2661 2535 784 360 314 245
NaeI GCC'GGC
Cuts at: 5080 5080
Size: 6899
NarI GG'CG_CC
Cuts at: 4330 4578 4330
Size: 248 6651
NcoI C'CATG_G
Cuts at: 369 2071 369
Size: 1702 5197
NdeI CA'TA_TG
Cuts at: 3351 3351
Size: 6899
NgoMIV G'CCGG_C
Cuts at: 5078 5078
Size: 6899
NsiI A_TGCA'T
Cuts at: 1549 1549
Size: 6899
NspI r_CATG'y
Cuts at: 662 1707 3952 4452 6055 662
Size: 1045 2245 500 1603 1506
Fragments arranged by size:
2245 1603 1506 1045 500
PciI A'CATG_T
Cuts at: 6051 6051
Size: 6899
PflMI CCAn_nnn'nTGG
Cuts at: 788 1805 3048 788
Size: 1017 1243 4639
Fragments arranged by size:
4639 1243 1017
PfoI T'CCnGG_A
Cuts at: 3404 3404
Size: 6899
PmeI GTTT'AAAC
Cuts at: 3898 3898
Size: 6899
PmlI CAC'GTG
Cuts at: 1465 1465
Size: 6899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5345 5377 5345
Size: 32 6867
PpuMI rG'GwC_Cy
Cuts at: 1413 1413
Size: 6899
PsiI TTA'TAA
Cuts at: 701 701
Size: 6899
PstI C_TGCA'G
Cuts at: 1770 3576 3946 4631 1770
Size: 1806 370 685 4038
Fragments arranged by size:
4038 1806 685 370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6899
PvuII CAG'CTG
Cuts at: 1349 4324 4684 1349
Size: 2975 360 3564
Fragments arranged by size:
3564 2975 360
RsrII CG'GwC_CG
Cuts at: 5094 5094
Size: 6899
SacI G_AGCT'C
Cuts at: 3913 3913
Size: 6899
SalI G'TCGA_C
Cuts at: 3936 3936
Size: 6899
SanDI GG'GwC_CC
Cuts at: 1413 1413
Size: 6899
SapI GCTCTTCn'nnn_
Cuts at: 4922 5132 4922
Size: 210 6689
SbfI CC_TGCA'GG
Cuts at: 3946 3946
Size: 6899
ScaI AGT'ACT
Cuts at: 3609 6416 3609
Size: 2807 4092
SfcI C'TryA_G
Cuts at: 33 1766 2753 3572 3634 3942 4627 5595 5786
Size: 1733 987 819 62 308 685 968 191
Cuts at: 5786 33
Size: 1146
Fragments arranged by size:
1733 1146 987 968 819 685 308 191
62
SfoI GGC'GCC
Cuts at: 4331 4579 4331
Size: 248 6651
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6899
SmaI CCC'GGG
Cuts at: 3921 3921
Size: 6899
SmlI C'TyrA_G
Cuts at: 2053 4054 4969 5683 5945 2053
Size: 2001 915 714 262 3007
Fragments arranged by size:
3007 2001 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6899
SphI G_CATG'C
Cuts at: 1707 3952 4452 1707
Size: 2245 500 4154
Fragments arranged by size:
4154 2245 500
StuI AGG'CCT
Cuts at: 617 617
Size: 6899
StyI C'CwwG_G
Cuts at: 369 2071 3729 3752 4033 4387 369
Size: 1702 1658 23 281 354 2881
Fragments arranged by size:
2881 1702 1658 354 281 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2633 3739 4482 4615 5074 5283 2633
Size: 1106 743 133 459 209 4249
Fragments arranged by size:
4249 1106 743 459 209 133
TatI w'GTAC_w
Cuts at: 971 2061 2951 3140 3607 6414 971
Size: 1090 890 189 467 2807 1456
Fragments arranged by size:
2807 1456 1090 890 467 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1199 1231 6416 6448 1199
Size: 32 5185 32 1650
Fragments arranged by size:
5185 1650 32 32
Tth111I GACn'n_nGTC
Cuts at: 4696 4696
Size: 6899
XbaI T'CTAG_A
Cuts at: 3930 3930
Size: 6899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4394 4394
Size: 6899
XmaI C'CCGG_G
Cuts at: 3919 3919
Size: 6899
XmnI GAAnn'nnTTC
Cuts at: 941 941
Size: 6899
ZraI GAC'GTC
Cuts at: 2049 2849 2049
Size: 800 6099
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AloI ApaI AscI AvrII BmgBI
BmtI BsiWI BsmBI BspEI BssHII BstEII BstZ17I ClaI
CspCI EcoNI FseI FspAI HindIII HpaI NheI NotI
NruI PacI PasI PshAI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI