Restriction map for
pF1KB4035
(Circular) (Six-base) MAPSORT of: pF1KB4035.seq Check: 3372 from: 1 to: 4532
pF1KB4035 4532 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 929 1570 929
Size: 641 3891
Acc65I G'GTAC_C
Cuts at: 1066 1548 1066
Size: 482 4050
AclI AA'CG_TT
Cuts at: 4372 4372
Size: 4532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 2357 2789 3536 3804 1532
Size: 825 432 747 268 2260
Fragments arranged by size:
2260 825 747 432 268
AfeI AGC'GCT
Cuts at: 1128 1128
Size: 4532
AflIII A'CryG_T
Cuts at: 1884 2988 3965 1884
Size: 1104 977 2451
Fragments arranged by size:
2451 1104 977
AgeI A'CCGG_T
Cuts at: 2920 2920
Size: 4532
AhdI GACnn_n'nnGTC
Cuts at: 720 720
Size: 4532
AlwNI CAG_nnn'CTG
Cuts at: 919 1150 1288 1837 3404 919
Size: 231 138 549 1567 2047
Fragments arranged by size:
2047 1567 549 231 138
ApaLI G'TGCA_C
Cuts at: 3302 3302
Size: 4532
ApoI r'AATT_y
Cuts at: 181 840 1432 1536 3885 4141 181
Size: 659 592 104 2349 256 572
Fragments arranged by size:
2349 659 592 572 256 104
AseI AT'TA_AT
Cuts at: 20 20
Size: 4532
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
AvaI C'yCGr_G
Cuts at: 1552 1552
Size: 4532
BamHI G'GATC_C
Cuts at: 1557 1557
Size: 4532
BanI G'GyrC_C
Cuts at: 733 1066 1548 1962 2210 2245 733
Size: 333 482 414 248 35 3020
Fragments arranged by size:
3020 482 414 333 248 35
BanII G_rGCy'C
Cuts at: 1546 2576 1546
Size: 1030 3502
BbeI G_GCGC'C
Cuts at: 1966 2214 1966
Size: 248 4284
BciVI GTATCCnnnnn_n'
Cuts at: 2430 3191 4532 2430
Size: 761 1341 2430
Fragments arranged by size:
2430 1341 761
BclI T'GATC_A
Cuts at: 242 488 2052 242
Size: 246 1564 2722
Fragments arranged by size:
2722 1564 246
BglI GCCn_nnn'nGGC
Cuts at: 389 389
Size: 4532
BglII A'GATC_T
Cuts at: 867 1360 2047 4084 867
Size: 493 687 2037 1315
Fragments arranged by size:
2037 1315 687 493
BlpI GC'TnA_GC
Cuts at: 211 1644 211
Size: 1433 3099
Bme1580I G_kGCm'C
Cuts at: 2157 2250 3306 2157
Size: 93 1056 3383
Fragments arranged by size:
3383 1056 93
BmgBI CAC'GTC
Cuts at: 722 722
Size: 4532
BmrI ACTGGGnnnn_n'
Cuts at: 544 607 957 982 1744 1825 2160 4304 544
Size: 63 350 25 762 81 335 2144 772
Fragments arranged by size:
2144 772 762 350 335 81 63 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 4532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1398 1708 2587 3079 3377 292
Size: 1106 310 879 492 298 1447
Fragments arranged by size:
1447 1106 879 492 310 298
BsaI GGTCTCn'nnnn_
Cuts at: 707 792 1298 4236 707
Size: 85 506 2938 1003
Fragments arranged by size:
2938 1003 506 85
BsaAI yAC'GTr
Cuts at: 1885 1885
Size: 4532
BsaBI GATnn'nnATC
Cuts at: 6 479 1041 2046 4083 6
Size: 473 562 1005 2037 455
Fragments arranged by size:
2037 1005 562 473 455
BsaHI Gr'CG_yC
Cuts at: 1963 2211 2913 4409 1963
Size: 248 702 1496 2086
Fragments arranged by size:
2086 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1424 1721 2242 2920 3194 3341 3752 1424
Size: 297 521 678 274 147 411 2204
Fragments arranged by size:
2204 678 521 411 297 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 854 884 854
Size: 30 4502
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 818 1286 818
Size: 468 4064
BseYI C'CCAG_C
Cuts at: 1957 2715 3292 3788 1957
Size: 758 577 496 2701
Fragments arranged by size:
2701 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 749 761 1109 704
Size: 45 12 348 4127
Fragments arranged by size:
4127 348 45 12
BsiEI CG_ry'CG
Cuts at: 104 1413 2074 2120 3328 104
Size: 1309 661 46 1208 1308
Fragments arranged by size:
1309 1308 1208 661 46
BsiHKAI G_wGCw'C
Cuts at: 1546 2324 2514 3306 1546
Size: 778 190 792 2772
Fragments arranged by size:
2772 792 778 190
BsiWI C'GTAC_G
Cuts at: 1397 1397
Size: 4532
BsmBI CGTCTCn'nnnn_
Cuts at: 358 358
Size: 4532
Bsp1286I G_dGCh'C
Cuts at: 1546 2157 2250 2324 2514 2576 3306 1546
Size: 611 93 74 190 62 730 2772
Fragments arranged by size:
2772 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3752 3752
Size: 4532
BspHI T'CATG_A
Cuts at: 3708 3708
Size: 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 676 941 1582 1796 2098 2479 676
Size: 265 641 214 302 381 2729
Fragments arranged by size:
2729 641 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 2824 2878 4362 4527 50
Size: 2774 54 1484 165 55
Fragments arranged by size:
2774 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2444 2444
Size: 4532
BsrFI r'CCGG_y
Cuts at: 2530 2711 2920 2530
Size: 181 209 4142
Fragments arranged by size:
4142 209 181
BsrGI T'GTAC_A
Cuts at: 575 575
Size: 4532
BssSI C'ACGA_G
Cuts at: 1348 2803 3161 1348
Size: 1455 358 2719
Fragments arranged by size:
2719 1455 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1909 1909
Size: 4532
BstBI TT'CG_AA
Cuts at: 2893 2893
Size: 4532
BstYI r'GATC_y
Cuts at: 1 867 1360 1557 2024 2047 2382 2628 3640
Size: 866 493 197 467 23 335 246 1012
Cuts at: 3640 3726 3738 4084 1
Size: 86 12 346 449
Fragments arranged by size:
1012 866 493 467 449 346 335 246
197 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4532
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2482 2663 2826 2482
Size: 181 163 4188
Fragments arranged by size:
4188 181 163
BtsI GCAGTG_nn'
Cuts at: 1775 1775
Size: 4532
DraI TTT'AAA
Cuts at: 1531 1531
Size: 4532
DrdI GACnn_nn'nnGTC
Cuts at: 2238 3096 2238
Size: 858 3674
EaeI y'GGCC_r
Cuts at: 2117 2291 2682 2709 4054 2117
Size: 174 391 27 1345 2595
Fragments arranged by size:
2595 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2117 2117
Size: 4532
EarI CTCTTCn'nnn_
Cuts at: 1136 2555 2765 1136
Size: 1419 210 2903
Fragments arranged by size:
2903 1419 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2740 3050 3196 4342 2740
Size: 310 146 1146 2930
Fragments arranged by size:
2930 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1544 1544
Size: 4532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 2357 2789 3536 3804 1532
Size: 825 432 747 268 2260
Fragments arranged by size:
2260 825 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 381 1671 381
Size: 1290 3242
EcoRI G'AATT_C
Cuts at: 1432 1536 1432
Size: 104 4428
EcoRV GAT'ATC
Cuts at: 1039 1039
Size: 4532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4532
FspI TGC'GCA
Cuts at: 2313 2313
Size: 4532
HaeII r_GCGC'y
Cuts at: 1130 1966 2214 3236 4215 1130
Size: 836 248 1022 979 1447
Fragments arranged by size:
1447 1022 979 836 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 738 1084 1116 1227 1259 2374 2404 2406
Size: 32 346 32 111 32 1115 30 2
Cuts at: 2406 2436 706
Size: 30 2802
Fragments arranged by size:
2802 1115 346 111 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 930 1371 1571 930
Size: 441 200 3891
Fragments arranged by size:
3891 441 200
KasI G'GCGC_C
Cuts at: 1962 2210 1962
Size: 248 4284
KpnI G_GTAC'C
Cuts at: 1070 1552 1070
Size: 482 4050
MluI A'CGCG_T
Cuts at: 3965 3965
Size: 4532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3203 3387 3203
Size: 184 4348
MscI TGG'CCA
Cuts at: 2293 2293
Size: 4532
MslI CAynn'nnrTG
Cuts at: 289 550 2521 2648 289
Size: 261 1971 127 2173
Fragments arranged by size:
2173 1971 261 127
MspA1I CmG'CkG
Cuts at: 1385 1643 1957 2317 3330 3575 1385
Size: 258 314 360 1013 245 2342
Fragments arranged by size:
2342 1013 360 314 258 245
NaeI GCC'GGC
Cuts at: 2713 2713
Size: 4532
NarI GG'CG_CC
Cuts at: 1963 2211 1963
Size: 248 4284
NcoI C'CATG_G
Cuts at: 107 107
Size: 4532
NdeI CA'TA_TG
Cuts at: 1062 1062
Size: 4532
NgoMIV G'CCGG_C
Cuts at: 2711 2711
Size: 4532
NspI r_CATG'y
Cuts at: 1182 1503 1585 2085 2992 1182
Size: 321 82 500 907 2722
Fragments arranged by size:
2722 907 500 321 82
PciI A'CATG_T
Cuts at: 2988 2988
Size: 4532
PmeI GTTT'AAAC
Cuts at: 1531 1531
Size: 4532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4500
PstI C_TGCA'G
Cuts at: 641 1579 2264 641
Size: 938 685 2909
Fragments arranged by size:
2909 938 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4532
PvuII CAG'CTG
Cuts at: 1385 1957 2317 1385
Size: 572 360 3600
Fragments arranged by size:
3600 572 360
RsrII CG'GwC_CG
Cuts at: 2727 2727
Size: 4532
SacI G_AGCT'C
Cuts at: 1546 1546
Size: 4532
SalI G'TCGA_C
Cuts at: 928 1569 928
Size: 641 3891
SapI GCTCTTCn'nnn_
Cuts at: 1136 2555 2765 1136
Size: 1419 210 2903
Fragments arranged by size:
2903 1419 210
SbfI CC_TGCA'GG
Cuts at: 1579 1579
Size: 4532
ScaI AGT'ACT
Cuts at: 1075 4049 1075
Size: 2974 1558
SexAI A'CCwGG_T
Cuts at: 774 792 774
Size: 18 4514
SfcI C'TryA_G
Cuts at: 33 637 1386 1575 2260 3253 3444 33
Size: 604 749 189 685 993 191 1121
Fragments arranged by size:
1121 993 749 685 604 191 189
SfoI GGC'GCC
Cuts at: 1964 2212 1964
Size: 248 4284
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
SmaI CCC'GGG
Cuts at: 1554 1554
Size: 4532
SmlI C'TyrA_G
Cuts at: 307 1377 1687 2602 3094 3356 307
Size: 1070 310 915 492 262 1483
Fragments arranged by size:
1483 1070 915 492 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4532
SphI G_CATG'C
Cuts at: 1503 1585 2085 1503
Size: 82 500 3950
Fragments arranged by size:
3950 500 82
StyI C'CwwG_G
Cuts at: 107 1137 1666 2020 107
Size: 1030 529 354 2619
Fragments arranged by size:
2619 1030 529 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 697 1427 2115 2248 2707 2916 697
Size: 730 688 133 459 209 2313
Fragments arranged by size:
2313 730 688 459 209 133
TatI w'GTAC_w
Cuts at: 311 320 455 575 1073 4047 311
Size: 9 135 120 498 2974 796
Fragments arranged by size:
2974 796 498 135 120 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4049 4081 4049
Size: 32 4500
Tth111I GACn'n_nGTC
Cuts at: 2329 2329
Size: 4532
XbaI T'CTAG_A
Cuts at: 1563 1563
Size: 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 288 1444 2027 288
Size: 1156 583 2793
Fragments arranged by size:
2793 1156 583
XmaI C'CCGG_G
Cuts at: 1552 1552
Size: 4532
XmnI GAAnn'nnTTC
Cuts at: 1432 1432
Size: 4532
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI