(Circular) (Six-base) MAPSORT of: pF1KB4038.seq Check: 94 from: 1 to: 4202 pF1KB4038 4202 bp With 182 enzymes: SgfI * April 24, 2009 13:02 .. AccI GT'mk_AC Cuts at: 737 1240 737 Size: 503 3699 Acc65I G'GTAC_C Cuts at: 1218 1218 Size: 4202 AclI AA'CG_TT Cuts at: 4042 4042 Size: 4202 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 2459 3206 3474 2027 Size: 432 747 268 2755 Fragments arranged by size: 2755 747 432 268 AflIII A'CryG_T Cuts at: 1554 2658 3635 1554 Size: 1104 977 2121 Fragments arranged by size: 2121 1104 977 AgeI A'CCGG_T Cuts at: 2590 2590 Size: 4202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 138 138 Size: 4202 AlwNI CAG_nnn'CTG Cuts at: 990 1507 3074 990 Size: 517 1567 2118 Fragments arranged by size: 2118 1567 517 ApaI G_GGCC'C Cuts at: 353 353 Size: 4202 ApaLI G'TGCA_C Cuts at: 2972 2972 Size: 4202 ApoI r'AATT_y Cuts at: 312 1206 3555 3811 312 Size: 894 2349 256 703 Fragments arranged by size: 2349 894 703 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4202 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4202 AvaI C'yCGr_G Cuts at: 1222 1222 Size: 4202 BamHI G'GATC_C Cuts at: 1227 1227 Size: 4202 BanI G'GyrC_C Cuts at: 1000 1218 1632 1880 1915 1000 Size: 218 414 248 35 3287 Fragments arranged by size: 3287 414 248 218 35 BanII G_rGCy'C Cuts at: 264 353 410 1216 2246 264 Size: 89 57 806 1030 2220 Fragments arranged by size: 2220 1030 806 89 57 BbeI G_GCGC'C Cuts at: 1636 1884 1636 Size: 248 3954 BbsI GAAGACnn'nnnn_ Cuts at: 1013 1179 1013 Size: 166 4036 BbvCI CC'TCA_GC Cuts at: 767 767 Size: 4202 BciVI GTATCCnnnnn_n' Cuts at: 2100 2861 4202 2100 Size: 761 1341 2100 Fragments arranged by size: 2100 1341 761 BclI T'GATC_A Cuts at: 1722 1722 Size: 4202 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 4202 BglII A'GATC_T Cuts at: 887 1058 1717 3754 887 Size: 171 659 2037 1335 Fragments arranged by size: 2037 1335 659 171 BlpI GC'TnA_GC Cuts at: 1314 1314 Size: 4202 Bme1580I G_kGCm'C Cuts at: 353 1827 1920 2976 353 Size: 1474 93 1056 1579 Fragments arranged by size: 1579 1474 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 774 1414 1495 1830 3974 774 Size: 640 81 335 2144 1002 Fragments arranged by size: 2144 1002 640 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 424 2569 424 Size: 2145 2057 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 591 763 868 967 1114 591 Size: 172 105 99 147 3679 Fragments arranged by size: 3679 172 147 105 99 Bpu10I CC'TnA_GC Cuts at: 575 767 575 Size: 192 4010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 453 1378 2257 2749 3047 453 Size: 925 879 492 298 1608 Fragments arranged by size: 1608 925 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 769 3906 769 Size: 3137 1065 BsaAI yAC'GTr Cuts at: 1555 1555 Size: 4202 BsaBI GATnn'nnATC Cuts at: 6 1716 3753 6 Size: 1710 2037 455 Fragments arranged by size: 2037 1710 455 BsaHI Gr'CG_yC Cuts at: 1633 1881 2583 4079 1633 Size: 248 702 1496 1756 Fragments arranged by size: 1756 1496 702 248 BsaWI w'CCGG_w Cuts at: 1190 1391 1912 2590 2864 3011 3422 1190 Size: 201 521 678 274 147 411 1970 Fragments arranged by size: 1970 678 521 411 274 201 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 620 650 1036 1066 620 Size: 30 386 30 3756 Fragments arranged by size: 3756 386 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 169 418 1057 169 Size: 249 639 3314 Fragments arranged by size: 3314 639 249 BseYI C'CCAG_C Cuts at: 596 672 1627 2385 2962 3458 596 Size: 76 955 758 577 496 1340 Fragments arranged by size: 1340 955 758 577 496 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 635 635 Size: 4202 BsiEI CG_ry'CG Cuts at: 104 1744 1790 2998 104 Size: 1640 46 1208 1308 Fragments arranged by size: 1640 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 410 500 1216 1994 2184 2976 410 Size: 90 716 778 190 792 1636 Fragments arranged by size: 1636 792 778 716 190 90 Bsp1286I G_dGCh'C Cuts at: 264 353 410 500 1216 1827 1920 1994 2184 Size: 89 57 90 716 611 93 74 190 Cuts at: 2184 2246 2976 264 Size: 62 730 1490 Fragments arranged by size: 1490 730 716 611 190 93 90 89 74 62 57 BspEI T'CCGG_A Cuts at: 3422 3422 Size: 4202 BspHI T'CATG_A Cuts at: 410 3378 410 Size: 2968 1234 BspMI ACCTGCnnnn'nnnn_ Cuts at: 127 301 771 1252 1466 1768 2149 127 Size: 174 470 481 214 302 381 2180 Fragments arranged by size: 2180 481 470 381 302 214 174 BsrBI CCG'CTC Cuts at: 50 2494 2548 4032 4197 50 Size: 2444 54 1484 165 55 Fragments arranged by size: 2444 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2114 2114 Size: 4202 BsrFI r'CCGG_y Cuts at: 1157 2200 2381 2590 1157 Size: 1043 181 209 2769 Fragments arranged by size: 2769 1043 209 181 BssSI C'ACGA_G Cuts at: 545 2473 2831 545 Size: 1928 358 1916 Fragments arranged by size: 1928 1916 358 BstAPI GCAn_nnn'nTGC Cuts at: 1579 1579 Size: 4202 BstBI TT'CG_AA Cuts at: 2563 2563 Size: 4202 BstEII G'GTnAC_C Cuts at: 1110 1110 Size: 4202 BstYI r'GATC_y Cuts at: 1 887 1058 1227 1694 1717 2052 2298 3310 Size: 886 171 169 467 23 335 246 1012 Cuts at: 3310 3396 3408 3754 1 Size: 86 12 346 449 Fragments arranged by size: 1012 886 467 449 346 335 246 171 169 86 23 12 Bsu36I CC'TnA_GG Cuts at: 152 152 Size: 4202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2152 2333 2496 2152 Size: 181 163 3858 Fragments arranged by size: 3858 181 163 BtsI GCAGTG_nn' Cuts at: 1445 1445 Size: 4202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 237 272 237 Size: 35 4167 DraI TTT'AAA Cuts at: 1201 1201 Size: 4202 DrdI GACnn_nn'nnGTC Cuts at: 805 1908 2766 805 Size: 1103 858 2241 Fragments arranged by size: 2241 1103 858 EaeI y'GGCC_r Cuts at: 278 510 705 752 1101 1787 1961 2352 2379 Size: 232 195 47 349 686 174 391 27 Cuts at: 2379 3724 278 Size: 1345 756 Fragments arranged by size: 1345 756 686 391 349 232 195 174 47 27 EagI C'GGCC_G Cuts at: 1787 1787 Size: 4202 EarI CTCTTCn'nnn_ Cuts at: 482 648 2225 2435 482 Size: 166 1577 210 2249 Fragments arranged by size: 2249 1577 210 166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2410 2720 2866 4012 2410 Size: 310 146 1146 2600 Fragments arranged by size: 2600 1146 310 146 EcoICRI GAG'CTC Cuts at: 408 1214 408 Size: 806 3396 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 591 763 868 967 1114 2027 2459 3206 3474 Size: 172 105 99 147 913 432 747 268 Cuts at: 3474 591 Size: 1319 Fragments arranged by size: 1319 913 747 432 268 172 147 105 99 EcoO109I rG'GnC_Cy Cuts at: 159 350 821 1341 159 Size: 191 471 520 3020 Fragments arranged by size: 3020 520 471 191 EcoRI G'AATT_C Cuts at: 1206 1206 Size: 4202 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 4202 FspI TGC'GCA Cuts at: 1983 1983 Size: 4202 HaeII r_GCGC'y Cuts at: 1636 1884 2906 3885 1636 Size: 248 1022 979 1953 Fragments arranged by size: 1953 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 426 458 585 617 766 798 879 911 2044 Size: 32 127 32 149 32 81 32 1133 Cuts at: 2044 2074 2076 2106 426 Size: 30 2 30 2522 Fragments arranged by size: 2522 1133 149 127 81 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 909 1241 909 Size: 332 3870 KasI G'GCGC_C Cuts at: 1632 1880 1632 Size: 248 3954 KpnI G_GTAC'C Cuts at: 1222 1222 Size: 4202 MluI A'CGCG_T Cuts at: 3635 3635 Size: 4202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 221 2873 3057 221 Size: 2652 184 1366 Fragments arranged by size: 2652 1366 184 MscI TGG'CCA Cuts at: 512 707 754 1103 1963 512 Size: 195 47 349 860 2751 Fragments arranged by size: 2751 860 349 195 47 MslI CAynn'nnrTG Cuts at: 415 2191 2318 415 Size: 1776 127 2299 Fragments arranged by size: 2299 1776 127 MspA1I CmG'CkG Cuts at: 879 1313 1627 1987 3000 3245 879 Size: 434 314 360 1013 245 1836 Fragments arranged by size: 1836 1013 434 360 314 245 NaeI GCC'GGC Cuts at: 1159 2383 1159 Size: 1224 2978 NarI GG'CG_CC Cuts at: 1633 1881 1633 Size: 248 3954 NgoMIV G'CCGG_C Cuts at: 1157 2381 1157 Size: 1224 2978 NspI r_CATG'y Cuts at: 1186 1255 1755 2662 1186 Size: 69 500 907 2726 Fragments arranged by size: 2726 907 500 69 PasI CC'CwG_GG Cuts at: 171 202 171 Size: 31 4171 PciI A'CATG_T Cuts at: 2658 2658 Size: 4202 PflMI CCAn_nnn'nTGG Cuts at: 990 990 Size: 4202 PmeI GTTT'AAAC Cuts at: 1201 1201 Size: 4202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3341 3373 3341 Size: 32 4170 PpuMI rG'GwC_Cy Cuts at: 159 159 Size: 4202 PspOMI G'GGCC_C Cuts at: 349 349 Size: 4202 PstI C_TGCA'G Cuts at: 1249 1934 1249 Size: 685 3517 PvuI CG_AT'CG Cuts at: 104 104 Size: 4202 PvuII CAG'CTG Cuts at: 879 1627 1987 879 Size: 748 360 3094 Fragments arranged by size: 3094 748 360 RsrII CG'GwC_CG Cuts at: 1193 2397 1193 Size: 1204 2998 SacI G_AGCT'C Cuts at: 410 1216 410 Size: 806 3396 SalI G'TCGA_C Cuts at: 1239 1239 Size: 4202 SapI GCTCTTCn'nnn_ Cuts at: 482 2225 2435 482 Size: 1743 210 2249 Fragments arranged by size: 2249 1743 210 SbfI CC_TGCA'GG Cuts at: 1249 1249 Size: 4202 ScaI AGT'ACT Cuts at: 3719 3719 Size: 4202 SexAI A'CCwGG_T Cuts at: 989 989 Size: 4202 SfcI C'TryA_G Cuts at: 33 386 1245 1930 2923 3114 33 Size: 353 859 685 993 191 1121 Fragments arranged by size: 1121 993 859 685 353 191 SfoI GGC'GCC Cuts at: 1634 1882 1634 Size: 248 3954 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4202 SmaI CCC'GGG Cuts at: 1224 1224 Size: 4202 SmlI C'TyrA_G Cuts at: 432 1357 2272 2764 3026 432 Size: 925 915 492 262 1608 Fragments arranged by size: 1608 925 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4202 SphI G_CATG'C Cuts at: 1255 1755 1255 Size: 500 3702 StuI AGG'CCT Cuts at: 190 586 863 190 Size: 396 277 3529 Fragments arranged by size: 3529 396 277 StyI C'CwwG_G Cuts at: 507 1336 1690 507 Size: 829 354 3019 Fragments arranged by size: 3019 829 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 645 1089 1785 1918 2377 2586 645 Size: 444 696 133 459 209 2261 Fragments arranged by size: 2261 696 459 444 209 133 TatI w'GTAC_w Cuts at: 3717 3717 Size: 4202 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 620 651 652 683 1036 1068 3719 3751 620 Size: 31 1 31 353 32 2651 32 1071 Fragments arranged by size: 2651 1071 353 32 32 31 31 1 Tth111I GACn'n_nGTC Cuts at: 780 1999 780 Size: 1219 2983 XbaI T'CTAG_A Cuts at: 1233 1233 Size: 4202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 343 1697 343 Size: 1354 2848 XmaI C'CCGG_G Cuts at: 1222 1222 Size: 4202 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI