Restriction map for
pF1KB4041
(Circular) (Six-base) MAPSORT of: pF1KB4041.seq Check: 8136 from: 1 to: 4502
pF1KB4041 4502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1540 1540
Size: 4502
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2759 3112 3774 2327
Size: 432 353 662 3055
Fragments arranged by size:
3055 662 432 353
AfeI AGC'GCT
Cuts at: 388 388
Size: 4502
AflIII A'CryG_T
Cuts at: 1854 3654 3935 1854
Size: 1800 281 2421
Fragments arranged by size:
2421 1800 281
AgeI A'CCGG_T
Cuts at: 1072 1072
Size: 4502
AleI CACnn'nnGTG
Cuts at: 989 1073 1493 989
Size: 84 420 3998
Fragments arranged by size:
3998 420 84
AlwNI CAG_nnn'CTG
Cuts at: 1807 3245 1807
Size: 1438 3064
ApaLI G'TGCA_C
Cuts at: 1176 3340 1176
Size: 2164 2338
ApoI r'AATT_y
Cuts at: 318 492 644 896 980 1316 1506 3855 4111
Size: 174 152 252 84 336 190 2349 256
Cuts at: 4111 318
Size: 709
Fragments arranged by size:
2349 709 336 256 252 190 174 152
84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 1034 1522 1034
Size: 488 4014
BamHI G'GATC_C
Cuts at: 1484 1527 1484
Size: 43 4459
BanI G'GyrC_C
Cuts at: 454 1518 1932 2180 2215 454
Size: 1064 414 248 35 2741
Fragments arranged by size:
2741 1064 414 248 35
BanII G_rGCy'C
Cuts at: 706 797 1516 2546 706
Size: 91 719 1030 2662
Fragments arranged by size:
2662 1030 719 91
BbeI G_GCGC'C
Cuts at: 1936 2184 1936
Size: 248 4254
BbsI GAAGACnn'nnnn_
Cuts at: 862 862
Size: 4502
BciVI GTATCCnnnnn_n'
Cuts at: 1142 2400 3456 4502 1142
Size: 1258 1056 1046 1142
Fragments arranged by size:
1258 1142 1056 1046
BclI T'GATC_A
Cuts at: 416 2022 416
Size: 1606 2896
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 1180 2127 2220 3344 1180
Size: 947 93 1124 2338
Fragments arranged by size:
2338 1124 947 93
BmgBI CAC'GTC
Cuts at: 207 207
Size: 4502
BmrI ACTGGGnnnn_n'
Cuts at: 210 662 830 1714 1795 2130 4274 210
Size: 452 168 884 81 335 2144 438
Fragments arranged by size:
2144 884 452 438 335 168 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 375 235
Size: 140 4362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 1678 2557 3271 3569 690
Size: 988 879 714 298 1623
Fragments arranged by size:
1623 988 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 240 4206 240
Size: 3966 536
BsaAI yAC'GTr
Cuts at: 1855 1855
Size: 4502
BsaBI GATnn'nnATC
Cuts at: 6 418 2016 4053 6
Size: 412 1598 2037 455
Fragments arranged by size:
2037 1598 455 412
BsaHI Gr'CG_yC
Cuts at: 1933 2181 2883 4379 1933
Size: 248 702 1496 2056
Fragments arranged by size:
2056 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1072 1691 2212 2890 3301 3448 3722 1072
Size: 619 521 678 411 147 274 1852
Fragments arranged by size:
1852 678 619 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4502
BseYI C'CCAG_C
Cuts at: 1118 1927 2685 3350 3758 1118
Size: 809 758 665 408 1862
Fragments arranged by size:
1862 809 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2044 2090 3320 104
Size: 1940 46 1230 1286
Fragments arranged by size:
1940 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 797 1180 1516 2294 2484 3344 797
Size: 383 336 778 190 860 1955
Fragments arranged by size:
1955 860 778 383 336 190
BsmI GAATG_Cn'
Cuts at: 844 844
Size: 4502
Bsp1286I G_dGCh'C
Cuts at: 706 797 1180 1516 2127 2220 2294 2484 2546
Size: 91 383 336 611 93 74 190 62
Cuts at: 2546 3344 706
Size: 798 1864
Fragments arranged by size:
1864 798 611 383 336 190 93 91
74 62
BspHI T'CATG_A
Cuts at: 294 2934 294
Size: 2640 1862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 447 1552 1766 2068 2449 447
Size: 1105 214 302 381 2500
Fragments arranged by size:
2500 1105 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2794 2848 4332 4497 50
Size: 2744 54 1484 165 55
Fragments arranged by size:
2744 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2414 2414
Size: 4502
BsrFI r'CCGG_y
Cuts at: 1072 2500 2681 1072
Size: 1428 181 2893
Fragments arranged by size:
2893 1428 181
BsrGI T'GTAC_A
Cuts at: 1345 1345
Size: 4502
BssSI C'ACGA_G
Cuts at: 1141 1225 1300 2773 3481 1141
Size: 84 75 1473 708 2162
Fragments arranged by size:
2162 1473 708 84 75
BstAPI GCAn_nnn'nTGC
Cuts at: 1879 1879
Size: 4502
BstBI TT'CG_AA
Cuts at: 2863 2863
Size: 4502
BstYI r'GATC_y
Cuts at: 1 1064 1484 1527 1994 2017 2352 2598 2904
Size: 1063 420 43 467 23 335 246 306
Cuts at: 2904 2916 3002 4054 1
Size: 12 86 1052 449
Fragments arranged by size:
1063 1052 467 449 420 335 306 246
86 43 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2452 2633 2796 2452
Size: 181 163 4158
Fragments arranged by size:
4158 181 163
BtsI GCAGTG_nn'
Cuts at: 1745 1745
Size: 4502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 831 866 831
Size: 35 4467
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3552 2208
Size: 1344 3158
EaeI y'GGCC_r
Cuts at: 345 1374 2087 2261 2652 2679 4024 345
Size: 1029 713 174 391 27 1345 823
Fragments arranged by size:
1345 1029 823 713 391 174 27
EagI C'GGCC_G
Cuts at: 2087 2087
Size: 4502
EarI CTCTTCn'nnn_
Cuts at: 1354 2525 2735 1354
Size: 1171 210 3121
Fragments arranged by size:
3121 1171 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2710 3452 3598 4312 2710
Size: 742 146 714 2900
Fragments arranged by size:
2900 742 714 146
EcoICRI GAG'CTC
Cuts at: 795 1514 795
Size: 719 3783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 375 2327 2759 3112 3774 235
Size: 140 1952 432 353 662 963
Fragments arranged by size:
1952 963 662 432 353 140
EcoO109I rG'GnC_Cy
Cuts at: 192 1641 192
Size: 1449 3053
EcoRI G'AATT_C
Cuts at: 318 492 896 980 1316 1506 318
Size: 174 404 84 336 190 3314
Fragments arranged by size:
3314 404 336 190 174 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 388 624 2521 388
Size: 236 1897 2369
Fragments arranged by size:
2369 1897 236
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 390 1936 2184 3414 4185 390
Size: 1546 248 1230 771 707
Fragments arranged by size:
1546 1230 771 707 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 1283 1315 1353 1385 2344 2374 2376
Size: 32 1016 32 38 32 959 30 2
Cuts at: 2376 2406 235
Size: 30 2331
Fragments arranged by size:
2331 1016 959 38 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 550 1541 550
Size: 991 3511
KasI G'GCGC_C
Cuts at: 1932 2180 1932
Size: 248 4254
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MfeI C'AATT_G
Cuts at: 1200 1200
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3261 3445 3261
Size: 184 4318
MscI TGG'CCA
Cuts at: 2263 2263
Size: 4502
MslI CAynn'nnrTG
Cuts at: 569 737 989 1073 1493 2491 2618 569
Size: 168 252 84 420 998 127 2453
Fragments arranged by size:
2453 998 420 252 168 127 84
MspA1I CmG'CkG
Cuts at: 1613 1927 2287 3071 3316 1613
Size: 314 360 784 245 2799
Fragments arranged by size:
2799 784 360 314 245
NaeI GCC'GGC
Cuts at: 2683 2683
Size: 4502
NarI GG'CG_CC
Cuts at: 1933 2181 1933
Size: 248 4254
NcoI C'CATG_G
Cuts at: 107 107
Size: 4502
NdeI CA'TA_TG
Cuts at: 258 258
Size: 4502
NgoMIV G'CCGG_C
Cuts at: 2681 2681
Size: 4502
NspI r_CATG'y
Cuts at: 1451 1555 2055 3658 1451
Size: 104 500 1603 2295
Fragments arranged by size:
2295 1603 500 104
PasI CC'CwG_GG
Cuts at: 114 114
Size: 4502
PciI A'CATG_T
Cuts at: 3654 3654
Size: 4502
PflMI CCAn_nnn'nTGG
Cuts at: 120 120
Size: 4502
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 4470
PpuMI rG'GwC_Cy
Cuts at: 192 192
Size: 4502
PspXI vC'TCGA_Gb
Cuts at: 1034 1034
Size: 4502
PstI C_TGCA'G
Cuts at: 1549 2234 1549
Size: 685 3817
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 1927 2287 1927
Size: 360 4142
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 797 1516 797
Size: 719 3783
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 1354 2525 2735 1354
Size: 1171 210 3121
Fragments arranged by size:
3121 1171 210
SbfI CC_TGCA'GG
Cuts at: 1549 1549
Size: 4502
ScaI AGT'ACT
Cuts at: 4019 4019
Size: 4502
SfcI C'TryA_G
Cuts at: 33 222 278 1545 2230 3198 3389 33
Size: 189 56 1267 685 968 191 1146
Fragments arranged by size:
1267 1146 968 685 191 189 56
SfoI GGC'GCC
Cuts at: 1934 2182 1934
Size: 248 4254
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SgrAI Cr'CCGG_yG
Cuts at: 1072 1072
Size: 4502
SmaI CCC'GGG
Cuts at: 1524 1524
Size: 4502
SmlI C'TyrA_G
Cuts at: 705 1034 1657 2572 3286 3548 705
Size: 329 623 915 714 262 1659
Fragments arranged by size:
1659 915 714 623 329 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
StuI AGG'CCT
Cuts at: 523 607 775 1027 1111 1195 523
Size: 84 168 252 84 84 3830
Fragments arranged by size:
3830 252 168 84 84 84
StyI C'CwwG_G
Cuts at: 107 342 1636 1990 107
Size: 235 1294 354 2619
Fragments arranged by size:
2619 1294 354 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1331 2085 2218 2677 2886 1331
Size: 754 133 459 209 2947
Fragments arranged by size:
2947 754 459 209 133
TatI w'GTAC_w
Cuts at: 197 1345 4017 197
Size: 1148 2672 682
Fragments arranged by size:
2672 1148 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4470
Tth111I GACn'n_nGTC
Cuts at: 2299 2299
Size: 4502
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1997 1997
Size: 4502
XhoI C'TCGA_G
Cuts at: 1034 1034
Size: 4502
XmaI C'CCGG_G
Cuts at: 1522 1522
Size: 4502
XmnI GAAnn'nnTTC
Cuts at: 607 1195 1279 607
Size: 588 84 3830
Fragments arranged by size:
3830 588 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BspEI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI SwaI ZraI