Restriction map for
pF1KB4044
(Circular) (Six-base) MAPSORT of: pF1KB4044.seq Check: 6154 from: 1 to: 4661
pF1KB4044 4661 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 701 701
Size: 4661
AccI GT'mk_AC
Cuts at: 1699 1699
Size: 4661
Acc65I G'GTAC_C
Cuts at: 1677 1677
Size: 4661
AclI AA'CG_TT
Cuts at: 4501 4501
Size: 4661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 2918 3665 3933 2486
Size: 432 747 268 3214
Fragments arranged by size:
3214 747 432 268
AflIII A'CryG_T
Cuts at: 390 536 1480 2013 3117 4094 390
Size: 146 944 533 1104 977 957
Fragments arranged by size:
1104 977 957 944 533 146
AgeI A'CCGG_T
Cuts at: 3049 3049
Size: 4661
AlwNI CAG_nnn'CTG
Cuts at: 693 1624 1966 3533 693
Size: 931 342 1567 1821
Fragments arranged by size:
1821 1567 931 342
ApaLI G'TGCA_C
Cuts at: 1548 3431 1548
Size: 1883 2778
ApoI r'AATT_y
Cuts at: 416 964 1099 1665 4014 4270 416
Size: 548 135 566 2349 256 807
Fragments arranged by size:
2349 807 566 548 256 135
AseI AT'TA_AT
Cuts at: 20 200 20
Size: 180 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
AvaI C'yCGr_G
Cuts at: 1681 1681
Size: 4661
AvrII C'CTAG_G
Cuts at: 1573 1573
Size: 4661
BamHI G'GATC_C
Cuts at: 648 1686 648
Size: 1038 3623
BanI G'GyrC_C
Cuts at: 1677 2091 2339 2374 1677
Size: 414 248 35 3964
Fragments arranged by size:
3964 414 248 35
BanII G_rGCy'C
Cuts at: 855 1380 1612 1675 2705 855
Size: 525 232 63 1030 2811
Fragments arranged by size:
2811 1030 525 232 63
BbeI G_GCGC'C
Cuts at: 2095 2343 2095
Size: 248 4413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4627
BciVI GTATCCnnnnn_n'
Cuts at: 2559 3320 4661 2559
Size: 761 1341 2559
Fragments arranged by size:
2559 1341 761
BclI T'GATC_A
Cuts at: 2181 2181
Size: 4661
BglII A'GATC_T
Cuts at: 923 2176 4213 923
Size: 1253 2037 1371
Fragments arranged by size:
2037 1371 1253
BlpI GC'TnA_GC
Cuts at: 1080 1773 1080
Size: 693 3968
Bme1580I G_kGCm'C
Cuts at: 902 1552 2286 2379 3435 902
Size: 650 734 93 1056 2128
Fragments arranged by size:
2128 1056 734 650 93
BmgBI CAC'GTC
Cuts at: 537 537
Size: 4661
BmrI ACTGGGnnnn_n'
Cuts at: 1407 1873 1954 2289 4433 1407
Size: 466 81 335 2144 1635
Fragments arranged by size:
2144 1635 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 4661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 664 166
Size: 498 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 1837 2716 3208 3506 1231
Size: 606 879 492 298 2386
Fragments arranged by size:
2386 879 606 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4365 4365
Size: 4661
BsaAI yAC'GTr
Cuts at: 2014 2014
Size: 4661
BsaBI GATnn'nnATC
Cuts at: 6 2175 4212 6
Size: 2169 2037 455
Fragments arranged by size:
2169 2037 455
BsaHI Gr'CG_yC
Cuts at: 2092 2340 3042 4538 2092
Size: 248 702 1496 2215
Fragments arranged by size:
2215 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1850 2371 3049 3323 3470 3881 1850
Size: 521 678 274 147 411 2630
Fragments arranged by size:
2630 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 863 863
Size: 4661
BseYI C'CCAG_C
Cuts at: 205 1382 2086 2844 3421 3917 205
Size: 1177 704 758 577 496 949
Fragments arranged by size:
1177 949 758 704 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 679
Size: 4661
BsiEI CG_ry'CG
Cuts at: 104 2203 2249 3457 104
Size: 2099 46 1208 1308
Fragments arranged by size:
2099 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 469 620 855 1552 1675 2453 2643 3435 469
Size: 151 235 697 123 778 190 792 1695
Fragments arranged by size:
1695 792 778 697 235 190 151 123
BsmI GAATG_Cn'
Cuts at: 1546 1546
Size: 4661
Bsp1286I G_dGCh'C
Cuts at: 469 620 855 902 1380 1552 1612 1675 2286
Size: 151 235 47 478 172 60 63 611
Cuts at: 2286 2379 2453 2643 2705 3435 469
Size: 93 74 190 62 730 1695
Fragments arranged by size:
1695 730 611 478 235 190 172 151
93 74 63 62 60 47
BspEI T'CCGG_A
Cuts at: 3881 3881
Size: 4661
BspHI T'CATG_A
Cuts at: 3837 3837
Size: 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 701 1444 1711 1925 2227 2608 701
Size: 743 267 214 302 381 2754
Fragments arranged by size:
2754 743 381 302 267 214
BsrBI CCG'CTC
Cuts at: 50 1194 2953 3007 4491 4656 50
Size: 1144 1759 54 1484 165 55
Fragments arranged by size:
1759 1484 1144 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2573 2573
Size: 4661
BsrFI r'CCGG_y
Cuts at: 2659 2840 3049 2659
Size: 181 209 4271
Fragments arranged by size:
4271 209 181
BssSI C'ACGA_G
Cuts at: 2932 3290 2932
Size: 358 4303
BstAPI GCAn_nnn'nTGC
Cuts at: 1593 2038 1593
Size: 445 4216
BstBI TT'CG_AA
Cuts at: 714 3022 714
Size: 2308 2353
BstYI r'GATC_y
Cuts at: 1 648 659 923 1686 2153 2176 2511 2757
Size: 647 11 264 763 467 23 335 246
Cuts at: 2757 3769 3855 3867 4213 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 763 647 467 449 346 335 264
246 86 23 12 11
BtgI C'CryG_G
Cuts at: 902 902
Size: 4661
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2611 2792 2955 2611
Size: 181 163 4317
Fragments arranged by size:
4317 181 163
BtsI GCAGTG_nn'
Cuts at: 606 1904 606
Size: 1298 3363
DraI TTT'AAA
Cuts at: 182 443 509 1533 1660 182
Size: 261 66 1024 127 3183
Fragments arranged by size:
3183 1024 261 127 66
DrdI GACnn_nn'nnGTC
Cuts at: 2367 3225 2367
Size: 858 3803
EaeI y'GGCC_r
Cuts at: 591 1561 2246 2420 2811 2838 4183 591
Size: 970 685 174 391 27 1345 1069
Fragments arranged by size:
1345 1069 970 685 391 174 27
EagI C'GGCC_G
Cuts at: 2246 2246
Size: 4661
EarI CTCTTCn'nnn_
Cuts at: 1147 1268 2684 2894 1147
Size: 121 1416 210 2914
Fragments arranged by size:
2914 1416 210 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2869 3179 3325 4471 2869
Size: 310 146 1146 3059
Fragments arranged by size:
3059 1146 310 146
EcoICRI GAG'CTC
Cuts at: 853 1673 853
Size: 820 3841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 664 2486 2918 3665 3933 166
Size: 498 1822 432 747 268 894
Fragments arranged by size:
1822 894 747 498 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1513 1621 1800 1513
Size: 108 179 4374
Fragments arranged by size:
4374 179 108
EcoRI G'AATT_C
Cuts at: 1665 1665
Size: 4661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 535 805 2680 535
Size: 270 1875 2516
Fragments arranged by size:
2516 1875 270
FspI TGC'GCA
Cuts at: 2442 2442
Size: 4661
HaeII r_GCGC'y
Cuts at: 2095 2343 3365 4344 2095
Size: 248 1022 979 2412
Fragments arranged by size:
2412 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1339 1371 2503 2533 2535 2565 1339
Size: 32 1132 30 2 30 3435
Fragments arranged by size:
3435 1132 32 30 30 2
HincII GTy'rAC
Cuts at: 1125 1700 1125
Size: 575 4086
KasI G'GCGC_C
Cuts at: 2091 2339 2091
Size: 248 4413
KpnI G_GTAC'C
Cuts at: 1681 1681
Size: 4661
MluI A'CGCG_T
Cuts at: 4094 4094
Size: 4661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 331 3332 3516 331
Size: 3001 184 1476
Fragments arranged by size:
3001 1476 184
MscI TGG'CCA
Cuts at: 593 2422 593
Size: 1829 2832
MslI CAynn'nnrTG
Cuts at: 389 410 530 2650 2777 389
Size: 21 120 2120 127 2273
Fragments arranged by size:
2273 2120 127 120 21
MspA1I CmG'CkG
Cuts at: 1187 1382 1772 2086 2446 3459 3704 1187
Size: 195 390 314 360 1013 245 2144
Fragments arranged by size:
2144 1013 390 360 314 245 195
NaeI GCC'GGC
Cuts at: 2842 2842
Size: 4661
NarI GG'CG_CC
Cuts at: 2092 2340 2092
Size: 248 4413
NcoI C'CATG_G
Cuts at: 902 902
Size: 4661
NgoMIV G'CCGG_C
Cuts at: 2840 2840
Size: 4661
NspI r_CATG'y
Cuts at: 394 409 1484 1594 1714 2214 3121 394
Size: 15 1075 110 120 500 907 1934
Fragments arranged by size:
1934 1075 907 500 120 110 15
PciI A'CATG_T
Cuts at: 390 1480 3117 390
Size: 1090 1637 1934
Fragments arranged by size:
1934 1637 1090
PflMI CCAn_nnn'nTGG
Cuts at: 642 642
Size: 4661
PmeI GTTT'AAAC
Cuts at: 1660 1660
Size: 4661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4629
PstI C_TGCA'G
Cuts at: 492 726 1708 2393 492
Size: 234 982 685 2760
Fragments arranged by size:
2760 982 685 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4661
PvuII CAG'CTG
Cuts at: 2086 2446 2086
Size: 360 4301
RsrII CG'GwC_CG
Cuts at: 2856 2856
Size: 4661
SacI G_AGCT'C
Cuts at: 855 1675 855
Size: 820 3841
SalI G'TCGA_C
Cuts at: 1698 1698
Size: 4661
SapI GCTCTTCn'nnn_
Cuts at: 2684 2894 2684
Size: 210 4451
SbfI CC_TGCA'GG
Cuts at: 1708 1708
Size: 4661
ScaI AGT'ACT
Cuts at: 4178 4178
Size: 4661
SfcI C'TryA_G
Cuts at: 33 488 722 1704 2389 3382 3573 33
Size: 455 234 982 685 993 191 1121
Fragments arranged by size:
1121 993 982 685 455 234 191
SfoI GGC'GCC
Cuts at: 2093 2341 2093
Size: 248 4413
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
SmaI CCC'GGG
Cuts at: 1683 1683
Size: 4661
SmlI C'TyrA_G
Cuts at: 1246 1816 2731 3223 3485 1246
Size: 570 915 492 262 2422
Fragments arranged by size:
2422 915 570 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4661
SphI G_CATG'C
Cuts at: 1714 2214 1714
Size: 500 4161
StuI AGG'CCT
Cuts at: 274 274
Size: 4661
StyI C'CwwG_G
Cuts at: 132 902 1071 1217 1573 1795 2149 132
Size: 770 169 146 356 222 354 2644
Fragments arranged by size:
2644 770 356 354 222 169 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2244 2377 2836 3045 2244
Size: 133 459 209 3860
Fragments arranged by size:
3860 459 209 133
TatI w'GTAC_w
Cuts at: 238 1166 4176 238
Size: 928 3010 723
Fragments arranged by size:
3010 928 723
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 732 764 802 834 1531 1563 4178 4210 732
Size: 32 38 32 697 32 2615 32 1183
Fragments arranged by size:
2615 1183 697 38 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2458 2458
Size: 4661
XbaI T'CTAG_A
Cuts at: 1134 1692 1134
Size: 558 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 774 1249 2156 774
Size: 475 907 3279
Fragments arranged by size:
3279 907 475
XmaI C'CCGG_G
Cuts at: 1681 1681
Size: 4661
XmnI GAAnn'nnTTC
Cuts at: 1277 1277
Size: 4661
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI